LOCUS       pAAV.Syn.GCaMP6s.WPRE.SV40\(p2824)\Q        5932 bp    DNA     circular     18-OCT-2012
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|640264376|
COMMENT     VNTDBDATE|640264376|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV.Syn.GCaMP6s.WPRE.SV40 (p2824) Q|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAAV.Syn.GCaMP6s.WPRE.SV40 (p2824) Q" 0 5932 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "AatI" "AatII" "Acc65I" "AccI" "AclI" "Acu I" "AfeI" 
COMMENT         "AflII" "AflIII" "AgeI" "AhdI" "AleI" "AlwNI" "ApaI" "ApaLI" "AscI" 
COMMENT         "AseI" "AsiSI" "AvaI" "AvrII" "BaeI" "BamHI" "BanI" "BanII" "BbsI" 
COMMENT         "BbvCI" "BcgI" "BciVI" "BclI" "BfrBI" "BfuAI" "BglI" "BglII" "BlpI" 
COMMENT         "BmgBI" "BmrI" "BpmI" "Bpu10I" "BpuEI" "BsaAI" "BsaBI" "BsaHI" "BsaI" 
COMMENT         "BsaXI" "BseRI" "BseYI" "BsgI" "BsiEI" "BsiHKAI" "BsiWI" "BsmBI" "BsmI" 
COMMENT         "Bsp1286I" "BspDI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrFI" 
COMMENT         "BsrGI" "BssHII" "BssSI" "BstAPI" "BstBI" "BstEII" "BstXI" "BstYI" 
COMMENT         "BstZ17I" "Bsu36I" "BtgI" "BtgZ I" "BtsCI" "BtsI" "ClaI (m)" 
COMMENT         "ClaI (nm)" "DraI" "DraIII" "DrdI" "EaeI" "EagI" "EarI" "EciI" "EcoNI" 
COMMENT         "EcoO109I" "EcoRI" "EcoRV" "FseI" "FspI" "HaeII" "HincII" "HindIII" 
COMMENT         "HpaI" "I-SceI" "KasI" "KpnI" "MfeI" "MluI" "MscI" "MslI" "MspA1I" 
COMMENT         "NaeI" "NarI" "NcoI" "NdeI" "NgoMIV" "NheI" "NotI" "NruI" "NsiI" "NspI" 
COMMENT         "PacI" "PciI" "PflFI" "PflMI" "PI-SceI" "PmeI" "PmlI" "PpuMI" "PshAI" 
COMMENT         "PsiI" "PspOMI" "PstI" "PvuI" "PvuII" "RsrII" "SacI" "SacII" "SalI" 
COMMENT         "SapI" "SbfI" "ScaI" "SexAI" "SfcI" "SfiI" "SfoI" "SgrAI" "SmaI" "SmlI" 
COMMENT         "SnaBI" "SpeI" "SphI" "SrfI" "SspI" "StuI" "StyI" "SwaI" "Tth111I" 
COMMENT         "XbaI" "XcmI" "XhoI" "XmaI" "XmnI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 5932 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "AatI" "aggcct" 4 0 1 5921 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AatII" "gacgtc" 6 0 2 459 0 2515 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 1 1150 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "AccI" "gtmkac" 3 0 2 803 0 2661 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "AclI" "aacgtt" 3 0 2 4059 0 4432 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "Acu I" "ctgaag" 23 21 8 364 1 463 0 1318 0 
COMMENT            1362 1 1561 0 1637 0 3995 0 5007 1 0 "") 
COMMENT        #6=(CRSite (CStringList) "AfeI" "agcgct" 4 0 1 604 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "AflII" "cttaag" 2 0 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "AflIII" "acrygt" 2 0 1 5549 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "AgeI" "accggt" 2 0 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "AhdI" "gacnnnnngtc" 7 0 3 59 0 3053 0 4661 0 
COMMENT             0 "") 
COMMENT        #11=(CRSite (CStringList) "AleI" "cacnnnngtg" 6 0 3 1195 0 1345 0 1522 0 
COMMENT             0 "") 
COMMENT        #12=(CRSite (CStringList) "AlwNI" "cagnnnctg" 7 0 1 5140 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 202 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 3 2271 0 3989 0 5235 0 0 
COMMENT             "") #15=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "AseI" "attaat" 3 0 3 4486 0 5721 0 5780 0 0 
COMMENT             "") #17=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 6 29 0 40 0 871 0 2671 0 
COMMENT             3067 0 3078 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "BaeI" "acnnnngtayc" 24 19 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 681 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 7 252 0 588 0 1150 0 1200 
COMMENT             0 3477 0 4708 0 5805 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "BanII" "grgcyc" 6 0 4 202 0 868 0 2415 0 3447 
COMMENT             0 0 "") 
COMMENT        #24=(CRSite (CStringList) "BbsI" "gaagac" 9 13 1 2590 1 0 "") 
COMMENT        #25=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 1 435 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "BcgI" "cgannnnnntgc" 25 23 3 380 0 739 1 4123 
COMMENT             1 0 "") 
COMMENT        #27=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 3 2131 1 3824 0 5351 0 
COMMENT             0 "") 
COMMENT        #28=(CRSite (CStringList) "BclI" "tgatca" 2 0 2 1754 0 1973 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BfuAI" "acctgc" 11 15 1 1607 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 4 31 0 3083 0 3276 0 
COMMENT             4543 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BglII" "agatct" 2 0 1 185 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 1 862 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 1 814 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "BmrI" "actggg" 12 11 2 3166 0 4621 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "BpmI" "ctggag" 23 21 3 1114 0 1609 0 4592 0 0 
COMMENT             "") 
COMMENT        #37=(CRSite (CStringList) "Bpu10I" "cctnagc" 3 6 2 435 0 1033 0 0 "") 
COMMENT        #38=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 4 4057 0 4925 0 5166 1 
COMMENT             5464 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "BsaAI" "yacgtr" 4 0 4 167 0 1923 0 2944 0 
COMMENT             3518 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "BsaBI" "gatnnnnatc" 6 0 2 785 0 2921 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 6 45 0 456 0 589 0 2512 0 
COMMENT             3064 0 4121 0 0 "") 
COMMENT        #42=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 2 1718 0 4595 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "BsaXI" "acnnnnnctcc" 22 19 3 622 1 3557 1 
COMMENT             5711 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "BseRI" "gaggag" 17 15 4 646 0 1195 0 2187 1 
COMMENT             2248 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "BseYI" "cccagc" 2 6 3 524 0 1695 1 5245 0 0 
COMMENT             "") 
COMMENT        #46=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 5 979 0 1060 0 1293 1 
COMMENT             1390 0 1925 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "BsiEI" "cgrycg" 5 0 7 268 0 2680 0 3247 0 
COMMENT             4143 0 4292 0 5215 0 5639 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "BsiHKAI" "gwgcwc" 6 0 5 868 0 2275 0 3993 0 
COMMENT             4078 0 5239 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 2 2741 0 2834 1 0 "") 
COMMENT        #52=(CRSite (CStringList) "Bsp1286I" "gdgchc" 6 0 13 202 0 565 0 868 0 
COMMENT             1205 0 1334 0 1583 0 2275 0 2415 0 2428 0 3447 0 3993 0 4078 0 5239 
COMMENT             0 0 "") 
COMMENT        #53=(CRSite (CStringList) "BspDI" "atcgat" 3 0 4 1991 0 2057 0 2652 0 
COMMENT             2915 0 0 "") #54=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #55=(CRSite (CStringList) "BspHI" "tcatga" 2 0 2 3821 0 4829 0 0 "") 
COMMENT        #56=(CRSite (CStringList) "BspMI" "acctgc" 11 15 1 1607 0 0 "") 
COMMENT        #57=(CRSite (CStringList) "BsrBI" "gagcgg" 4 4 4 3374 1 3819 1 5620 1 
COMMENT             5861 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 4 1896 0 2309 1 4427 0 
COMMENT             4601 1 0 "") 
COMMENT        #59=(CRSite (CStringList) "BsrFI" "rccggy" 2 0 7 465 0 519 0 591 0 1315 
COMMENT             0 2574 0 3413 0 4576 0 0 "") 
COMMENT        #60=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 2 1139 0 2025 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 7 3 0 88 0 418 0 420 0 
COMMENT             448 0 3019 0 3104 0 0 "") 
COMMENT        #62=(CRSite (CStringList) "BssSI" "cacgag" 2 6 3 1345 1 3992 0 5376 0 0 
COMMENT             "") #63=(CRSite (CStringList) "BstAPI" "gcannnnntgc" 8 0 0 0 "") 
COMMENT        #64=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 1 1054 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 0 0 "") 
COMMENT        #66=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 1 1695 0 0 "") 
COMMENT        #67=(CRSite (CStringList) "BstYI" "rgatcy" 2 0 12 185 0 681 0 762 0 786 
COMMENT             0 2624 0 2922 0 4014 0 4031 0 4799 0 4811 0 4897 0 4908 0 0 "") 
COMMENT        #68=(CRSite (CStringList) "BstZ17I" "gtatac" 4 0 0 0 "") 
COMMENT        #69=(CRSite (CStringList) "Bsu36I" "cctnagg" 3 0 0 0 "") 
COMMENT        #70=(CRSite (CStringList) "BtgI" "ccrygg" 2 0 6 387 0 695 0 797 0 2366 0 
COMMENT             2438 0 2562 0 0 "") 
COMMENT        #71=(CRSite (CStringList) "BtgZ I" "gcgatg" 17 21 3 1286 0 2364 1 3513 1 
COMMENT             0 "") 
COMMENT        #72=(CRSite (CStringList) "BtsCI" "ggatgnn" 8 6 14 241 0 317 1 355 0 929 
COMMENT             1 1205 1 1442 1 1571 1 1677 0 1890 0 2004 0 3199 1 4215 0 4502 0 
COMMENT             4683 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "BtsI" "gcagtg" 9 7 6 369 1 1378 0 2746 0 4242 
COMMENT             0 4262 1 5769 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "ClaI (m)" "gatcgat" 4 6 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "ClaI (nm)" "hatcgatd" 4 0 4 1991 0 2057 0 
COMMENT             2652 0 2915 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "DraI" "tttaaa" 4 0 5 1638 0 2881 0 4083 0 
COMMENT             4775 0 4794 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "DraIII" "cacnnngtg" 7 0 1 3521 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "DrdI" "gacnnnnnngtc" 8 0 2 3565 0 5447 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "EaeI" "yggccr" 2 0 8 104 0 491 0 1236 0 2677 
COMMENT             0 3003 0 3132 0 4268 0 5710 0 0 "") 
COMMENT        #80=(CRSite (CStringList) "EagI" "cggccg" 2 0 1 2677 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "EarI" "ctcttc" 8 11 7 1613 1 1952 1 2012 1 
COMMENT             2594 0 3225 1 3862 1 5666 1 0 "") 
COMMENT        #82=(CRSite (CStringList) "EciI" "ggcgga" 18 16 6 922 1 1413 1 2386 0 
COMMENT             4519 0 5347 0 5493 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "EcoNI" "cctnnnnnagg" 6 0 1 5926 0 0 "") 
COMMENT        #84=(CRSite (CStringList) "EcoO109I" "rggnccy" 3 0 2 198 0 199 0 0 "") 
COMMENT        #85=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 662 0 0 "") 
COMMENT        #86=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "FseI" "ggccggcc" 7 0 0 0 "") 
COMMENT        #88=(CRSite (CStringList) "FspI" "tgcgca" 4 0 4 316 0 368 0 3266 0 4438 
COMMENT             0 0 "") 
COMMENT        #89=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 6 592 0 606 0 3363 0 3371 
COMMENT             0 5309 0 5679 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "HincII" "gtyrac" 4 0 4 804 0 1949 0 2662 0 
COMMENT             2820 0 0 "") 
COMMENT        #91=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2050 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "HpaI" "gttaac" 4 0 2 1949 0 2820 0 0 "") 
COMMENT        #93=(CRSite (CStringList) "I-SceI" "twgggataacagggtaat" 10 6 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "KasI" "ggcgcc" 2 0 1 588 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 1154 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "MfeI" "caattg" 2 0 1 2829 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "MluI" "acgcgt" 2 0 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "MscI" "tggcca" 4 0 2 106 0 3005 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "MslI" "caynnnnrtg" 6 0 7 719 0 1195 0 1345 0 
COMMENT             1522 0 3890 0 4249 0 4408 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "MspA1I" "cmgckg" 4 0 10 389 0 628 0 2399 0 
COMMENT              2548 0 2564 0 3216 0 4025 0 4966 0 5211 0 5729 0 0 "") 
COMMENT        #101=(CRSite (CStringList) "NaeI" "gccggc" 4 0 4 521 0 593 0 2576 0 3415 
COMMENT              0 0 "") 
COMMENT        #102=(CRSite (CStringList) "NarI" "ggcgcc" 3 0 1 589 0 0 "") 
COMMENT        #103=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 695 0 797 0 0 "") 
COMMENT        #104=(CRSite (CStringList) "NdeI" "catatg" 3 0 0 0 "") 
COMMENT        #105=(CRSite (CStringList) "NgoMIV" "gccggc" 2 0 4 519 0 591 0 2574 0 
COMMENT              3413 0 0 "") 
COMMENT        #106=(CRSite (CStringList) "NheI" "gctagc" 2 0 2 673 0 730 0 0 "") 
COMMENT        #107=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 2677 0 0 "") 
COMMENT        #108=(CRSite (CStringList) "NruI" "tcgcga" 4 0 0 0 "") 
COMMENT        #109=(CRSite (CStringList) "NsiI" "atgcat" 6 0 0 0 "") 
COMMENT        #110=(CRSite (CStringList) "NspI" "rcatgy" 6 0 1 5553 0 0 "") 
COMMENT        #111=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 2 3118 0 5916 0 0 "") 
COMMENT        #112=(CRSite (CStringList) "PciI" "acatgt" 2 0 1 5549 0 0 "") 
COMMENT        #113=(CRSite (CStringList) "PflFI" "gacnnngtc" 5 0 0 0 "") 
COMMENT        #114=(CRSite (CStringList) "PflMI" "ccannnnntgg" 8 0 1 2303 0 0 "") 
COMMENT        #115=(CRSite (CStringList) "PI-SceI" 
COMMENT              "atctatgtcgggtgcggagaaagaggtaatgaaatggca" 16 12 0 0 "") 
COMMENT        #116=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 0 0 "") 
COMMENT        #117=(CRSite (CStringList) "PmlI" "cacgtg" 4 0 1 1923 0 0 "") 
COMMENT        #118=(CRSite (CStringList) "PpuMI" "rggwccy" 3 0 0 0 "") 
COMMENT        #119=(CRSite (CStringList) "PshAI" "gacnnnngtc" 6 0 0 0 "") 
COMMENT        #120=(CRSite (CStringList) "PsiI" "ttataa" 4 0 2 2800 0 3646 0 0 "") 
COMMENT        #121=(CRSite (CStringList) "PspOMI" "gggccc" 2 0 1 198 0 0 "") 
COMMENT        #122=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 195 0 1748 0 0 "") 
COMMENT        #123=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 3247 0 4292 0 0 "") 
COMMENT        #124=(CRSite (CStringList) "PvuII" "cagctg" 4 0 2 3216 0 5729 0 0 "") 
COMMENT        #125=(CRSite (CStringList) "RsrII" "cggwccg" 3 0 1 531 0 0 "") 
COMMENT        #126=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 868 0 0 "") 
COMMENT        #127=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 2 390 0 2565 0 0 "") 
COMMENT        #128=(CRSite (CStringList) "SalI" "gtcgac" 2 0 2 802 0 2660 0 0 "") 
COMMENT        #129=(CRSite (CStringList) "SapI" "gctcttc" 9 12 2 1613 1 5666 1 0 "") 
COMMENT        #130=(CRSite (CStringList) "SbfI" "cctgcagg" 7 0 0 0 "") 
COMMENT        #131=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 4180 0 0 "") 
COMMENT        #132=(CRSite (CStringList) "SexAI" "accwggt" 2 0 0 0 "") 
COMMENT        #133=(CRSite (CStringList) "SfcI" "ctryag" 2 0 7 191 0 851 0 1744 0 3295 
COMMENT              0 4415 0 5093 0 5284 0 0 "") 
COMMENT        #134=(CRSite (CStringList) "SfiI" "ggccnnnnnggcc" 9 0 0 0 "") 
COMMENT        #135=(CRSite (CStringList) "SfoI" "ggcgcc" 4 0 1 590 0 0 "") 
COMMENT        #136=(CRSite (CStringList) "SgrAI" "crccggyg" 3 0 1 591 0 0 "") 
COMMENT        #137=(CRSite (CStringList) "SmaI" "cccggg" 4 0 5 31 0 42 0 2673 0 3069 0 
COMMENT              3080 0 0 "") 
COMMENT        #138=(CRSite (CStringList) "SmlI" "ctyrag" 2 0 5 871 0 4036 0 4904 0 
COMMENT              5181 0 5443 0 0 "") 
COMMENT        #139=(CRSite (CStringList) "SnaBI" "tacgta" 4 0 2 167 0 2944 0 0 "") 
COMMENT        #140=(CRSite (CStringList) "SpeI" "actagt" 2 0 0 0 "") 
COMMENT        #141=(CRSite (CStringList) "SphI" "gcatgc" 6 0 0 0 "") 
COMMENT        #142=(CRSite (CStringList) "SrfI" "gcccgggc" 5 0 4 31 0 42 0 3069 0 3080 
COMMENT              0 0 "") 
COMMENT        #143=(CRSite (CStringList) "SspI" "aatatt" 4 0 2 3726 0 3856 0 0 "") 
COMMENT        #144=(CRSite (CStringList) "StuI" "aggcct" 4 0 1 5921 0 0 "") 
COMMENT        #145=(CRSite (CStringList) "StyI" "ccwwgg" 2 0 6 497 0 695 0 797 0 1688 
COMMENT              0 1935 0 2470 0 0 "") 
COMMENT        #146=(CRSite (CStringList) "SwaI" "atttaaat" 5 0 0 0 "") 
COMMENT        #147=(CRSite (CStringList) "Tth111I" "gacnnngtc" 5 0 0 0 "") 
COMMENT        #148=(CRSite (CStringList) "XbaI" "tctaga" 2 0 0 0 "") 
COMMENT        #149=(CRSite (CStringList) "XcmI" "ccannnnnnnnntgg" 9 0 0 0 "") 
COMMENT        #150=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 871 0 0 "") 
COMMENT        #151=(CRSite (CStringList) "XmaI" "cccggg" 2 0 5 29 0 40 0 2671 0 3067 0 
COMMENT              3078 0 0 "") 
COMMENT        #152=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 1 4061 0 0 "")) 
COMMENT       (CObList 
COMMENT        #153=(CFSignal (CObList) "Amp(r)" 22 0 0 3873 4730 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #154=(CFSignal (CObList) "GCaMP6s" 4 0 0 696 2045 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #155=(CFSignal (CObList) "5' ITR" 34 0 0 1 167 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #156=(CFSignal (CObList) "3' ITR" 34 0 1 2943 3109 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #157=(CFSignal (CObList) "SV40 polyA" 25 0 0 2683 2914 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #158=(CFSignal (CObList) "Synapsin promoter" 29 0 0 190 661 0 
COMMENT              (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #159=(CFSignal (CObList) "WPRE" 22 0 0 2061 2649 0 (CStringList) 
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COMMENT                              "BciVI: 3 sites") 12 11 "GTATCCNNNNNNN" 
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COMMENT                              "BclI: 2 sites") 1 5 "TGATCA" "ACTAGT") 
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COMMENT                              "BfuAI: 1 site") 10 14 "ACCTGCNNNNNNNNN" 
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COMMENT                              "BglI: 4 sites") 7 4 "GCCNNNNNGGC" "CGGNNNNNCCG") 
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COMMENT                              "BglII: 1 site") 1 5 "AGATCT" "TCTAGA") 
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COMMENT                              "BlpI: 1 site") 2 5 "GCTNAGC" "CGANTCG") 
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COMMENT                              "BmgBI: 1 site") 3 3 "CACGTC" "GTGCAG") 
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COMMENT                              "BmrI: 2 sites") 11 10 "ACTGGGNNNNNN" 
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COMMENT                              "BpmI: 3 sites") 22 20 "CTGGAGNNNNNNNNNNNNNNNNN" 
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COMMENT                              "Bpu10I: 2 sites") 2 5 "CCTNAGC" "GGANTCG") 
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COMMENT                              "BpuEI: 4 sites") 22 20 "CTTGAGNNNNNNNNNNNNNNNNN" 
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COMMENT                              "BsaAI: 4 sites") 3 3 "YACGTR" "RTGCAY") 
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COMMENT                              "BsaBI: 2 sites") 5 5 "GATNNNNATC" "CTANNNNTAG") 
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COMMENT                              "BsaHI: 6 sites") 2 4 "GRCGYC" "CYGCRG") 
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COMMENT                              "BsaI: 2 sites") 7 11 "GGTCTCNNNNNN" 
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COMMENT                              "BsaXI: 3 sites") 21 18 "ACNNNNNCTCCNNNNNNNNNNN" 
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COMMENT                              "BseRI: 4 sites") 16 14 "GAGGAGNNNNNNNNNNN" 
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COMMENT                              "BseYI: 3 sites") 1 5 "CCCAGC" "GGGTCG") 
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COMMENT                              "BsgI: 5 sites") 22 20 "GTGCAGNNNNNNNNNNNNNNNNN" 
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COMMENT                              "BsiEI: 7 sites") 4 2 "CGRYCG" "GCYRGC") 
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COMMENT                              "BsiHKAI: 5 sites") 5 1 "GWGCWC" "CWCGWG") 
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COMMENT                              "Bsp1286I: 13 sites") 5 1 "GDGCHC" "CHCGDG") 
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COMMENT                              "BspDI: 4 sites") 2 4 "ATCGAT" "TAGCTA") 
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COMMENT                              "BspHI: 2 sites") 1 5 "TCATGA" "AGTACT") 
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COMMENT                                             " N2: 3819 (on complementary strand)" 
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COMMENT                             "GCAATGNNN" "CGTTACNNN") 
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COMMENT                              "BsrFI: 7 sites") 1 5 "RCCGGY" "YGGCCR") 
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COMMENT                             "GCGATGNNNNNNNNNNNNNNN" "CGCTACNNNNNNNNNNNNNNN") 
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COMMENT                                             " N6: 1442 (on complementary strand)" 
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COMMENT                                             " N7: 1571 (on complementary strand)" 
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COMMENT                              "BtsCI: 14 sites") 7 5 "GGATGNNN" "CCTACNNN") 
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COMMENT                                       #538=(CLinePar 
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COMMENT                                       #539=(CLinePar 
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COMMENT                                       #540=(CLinePar 
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COMMENT                                             " N5: 4262 (on complementary strand)" 
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COMMENT                              "BtsI: 6 sites") 8 6 "GCAGTGNNN" "CGTCACNNN") 
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COMMENT                              "ClaI (nm): 4 sites") 3 5 "HATCGATD" "DTAGCTAH") 
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COMMENT                              "DraI: 5 sites") 3 3 "TTTAAA" "AAATTT") 
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COMMENT                              "DraIII: 1 site") 6 3 "CACNNNGTG" "GTGNNNCAC") 
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COMMENT                              "DrdI: 2 sites") 7 5 "GACNNNNNNGTC" "CTGNNNNNNCAG") 
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COMMENT                              "EaeI: 8 sites") 1 5 "YGGCCR" "RCCGGY") 
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COMMENT                              "EagI: 1 site") 1 5 "CGGCCG" "GCCGGC") 
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COMMENT                             "TWGGGATAACAGGGTAAT" "AWCCCTATTGTCCCATTA") 
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COMMENT                              "KpnI: 1 site") 5 1 "GGTACC" "CCATGG") 
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COMMENT                              "MfeI: 1 site") 1 5 "CAATTG" "GTTAAC") 
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COMMENT                              "MscI: 2 sites") 3 3 "TGGCCA" "ACCGGT") 
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COMMENT                              "MslI: 7 sites") 5 5 "CAYNNNNRTG" "GTRNNNNYAC") 
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COMMENT                                              191) " N8: 4966 " 1) 
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COMMENT                              "MspA1I: 10 sites") 3 3 "CMGCKG" "GKCGMC") 
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COMMENT                              "NaeI: 4 sites") 3 3 "GCCGGC" "CGGCCG") 
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COMMENT                              "NarI: 1 site") 2 4 "GGCGCC" "CCGCGG") 
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COMMENT                              "NcoI: 2 sites") 1 5 "CCATGG" "GGTACC") 
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COMMENT                              "NgoMIV: 4 sites") 1 5 "GCCGGC" "CGGCCG") 
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COMMENT                              "NheI: 2 sites") 1 5 "GCTAGC" "CGATCG") 
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COMMENT                              "NotI: 1 site") 2 6 "GCGGCCGC" "CGCCGGCG") 
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COMMENT                             "AGCGCT") 
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COMMENT                               (CObjectList)) "NsiI: 0 sites") 5 1 "ATGCAT" 
COMMENT                             "TACGTA") 
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COMMENT                              "NspI: 1 site") 5 1 "RCATGY" "YGTACR") 
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COMMENT                              "PacI: 2 sites") 5 3 "TTAATTAA" "AATTAATT") 
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COMMENT                              "PciI: 1 site") 1 5 "ACATGT" "TGTACA") 
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COMMENT                             "CTGNNNCAG") 
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COMMENT                              "PflMI: 1 site") 7 4 "CCANNNNNTGG" "GGTNNNNNACC") 
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COMMENT                             "ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGGCA" 
COMMENT                             "TAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGT") 
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FEATURES             Location/Qualifiers
     misc_marker     3873..4730
                     /vntifkey="22"
                     /label=Amp(r)
     CDS             696..2045
                     /vntifkey="4"
                     /label=GCaMP6s
     repeat_region   1..167
                     /vntifkey="34"
                     /label=5'\ITR
     repeat_region   complement(2943..3109)
                     /vntifkey="34"
                     /label=3'\ITR
     polyA_signal    2683..2914
                     /vntifkey="25"
                     /label=SV40\polyA
     promoter        190..661
                     /vntifkey="29"
                     /label=Synapsin\promoter
     misc_marker     2061..2649
                     /vntifkey="22"
                     /label=WPRE
BASE COUNT     1391 a      1601 c      1581 g      1359 t 
ORIGIN
        1 tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc cgggcgtcgg gcgacctttg 
       61 gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta 
      121 ggggttcctt gtagttaatg attaacccgc catgctactt atctacgtag ccatgctcta 
      181 ggaagatctc tgcagagggc cctgcgtatg agtgcaagtg ggttttagga ccaggatgag 
      241 gcggggtggg ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc 
      301 cattccccaa attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg 
      361 cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc 
      421 gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac 
      481 tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca 
      541 cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc 
      601 agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc 
      661 gaattcaagc tgctagcaag gatccacccg ccaccatggg ttctcatcat catcatcatc 
      721 atggtatggc tagcatgact ggtggacagc aaatgggtcg ggatctgtac gacgatgacg 
      781 ataaggatct cgccaccatg gtcgactcat cacgtcgtaa gtggaataag acaggtcacg 
      841 cagtcagagc tataggtcgg ctgagctcac tcgagaacgt ctatatcaag gccgacaagc 
      901 agaagaacgg catcaaggcg aacttccaca tccgccacaa catcgaggac ggcggcgtgc 
      961 agctcgccta ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg 
     1021 acaaccacta cctgagcgtg cagtccaaac tttcgaaaga ccccaacgag aagcgcgatc 
     1081 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt 
     1141 acaagggcgg taccggaggg agcatggtga gcaagggcga ggagctgttc accggggtgg 
     1201 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg 
     1261 agggtgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca 
     1321 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca 
     1381 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct 
     1441 acatccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg 
     1501 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg 
     1561 aggacggcaa catcctgggg cacaagctgg agtacaacct gccggaccaa ctgactgaag 
     1621 agcagatcgc agaatttaaa gaggctttct ccctatttga caaggacggg gatgggacaa 
     1681 taacaaccaa ggagctgggg acggtgatgc ggtctctggg gcagaacccc acagaagcag 
     1741 agctgcagga catgatcaat gaagtagatg ccgacggtga cggcacaatc gacttccctg 
     1801 agttcctgac aatgatggca agaaaaatga aatacaggga cacggaagaa gaaattagag 
     1861 aagcgttcgg tgtgtttgat aaggatggca atggctacat cagtgcagca gagcttcgcc 
     1921 acgtgatgac aaaccttgga gagaagttaa cagatgaaga ggttgatgaa atgatcaggg 
     1981 aagcagacat cgatggggat ggtcaggtaa actacgaaga gtttgtacaa atgatgacag 
     2041 cgaagtgaaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg 
     2101 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt 
     2161 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc 
     2221 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt 
     2281 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga 
     2341 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct 
     2401 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat 
     2461 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct 
     2521 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc 
     2581 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg 
     2641 cctccccgca tcgataccgt cgacctcgac ccgggcggcc gcttcgagca gacatgataa 
     2701 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt 
     2761 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta 
     2821 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt 
     2881 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatcttcct agagcatggc 
     2941 tacgtagata agtagcatgg cgggttaatc attaactaca aggaacccct agtgatggag 
     3001 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc 
     3061 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ccttaattaa 
     3121 cctaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 
     3181 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc 
     3241 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc 
     3301 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 
     3361 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 
     3421 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 
     3481 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 
     3541 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 
     3601 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg 
     3661 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac 
     3721 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta 
     3781 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 
     3841 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 
     3901 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 
     3961 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 
     4021 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 
     4081 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 
     4141 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 
     4201 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 
     4261 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 
     4321 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 
     4381 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 
     4441 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 
     4501 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 
     4561 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 
     4621 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 
     4681 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 
     4741 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 
     4801 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 
     4861 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 
     4921 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 
     4981 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 
     5041 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 
     5101 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 
     5161 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 
     5221 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 
     5281 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 
     5341 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 
     5401 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 
     5461 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 
     5521 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 
     5581 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 
     5641 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 
     5701 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 
     5761 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 
     5821 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag 
     5881 gaaacagcta tgaccatgat tacgccagat ttaattaagg ccttaattag gc 
//
