LOCUS       pAAV.Syn.NES.jRCaMP1a.WPRE.SV40(p3855)        5657 bp    DNA     circular      7-JUL-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|737029950|
COMMENT     VNTDBDATE|738841046|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV.Syn.NES.jRCaMP1a.WPRE.SV40(p3855)|
COMMENT     VNTAUTHORNAME|Andres Fernandez|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAAV.Syn.NES.jRCaMP1a.WPRE.SV40(p3855)" 0 5657 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "AatI" "AatII" "Acc65I" "AccI" "AclI" "Acu I" "AfeI" 
COMMENT         "AflII" "AflIII" "AgeI" "AhdI" "AleI" "AlwNI" "ApaI" "ApaLI" "AscI" 
COMMENT         "AseI" "AsiSI" "AvaI" "AvrII" "BaeI" "BamHI" "BanI" "BanII" "BbsI" 
COMMENT         "BbvCI" "BcgI" "BciVI" "BclI" "BfrBI" "BfuAI" "BglI" "BglII" "BlpI" 
COMMENT         "BmgBI" "BmrI" "BpmI" "Bpu10I" "BpuEI" "BsaAI" "BsaBI" "BsaHI" "BsaI" 
COMMENT         "BsaXI" "BseRI" "BseYI" "BsgI" "BsiEI" "BsiHKAI" "BsiWI" "BsmBI" "BsmI" 
COMMENT         "Bsp1286I" "BspDI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrFI" 
COMMENT         "BsrGI" "BssHII" "BssSI" "BstAPI" "BstBI" "BstEII" "BstXI" "BstYI" 
COMMENT         "BstZ17I" "Bsu36I" "BtgI" "BtgZ I" "BtsCI" "BtsI" "ClaI (m)" 
COMMENT         "ClaI (nm)" "DraI" "DraIII" "DrdI" "EaeI" "EagI" "EarI" "EciI" "EcoNI" 
COMMENT         "EcoO109I" "EcoRI" "EcoRV" "FseI" "FspI" "HaeII" "HincII" "HindIII" 
COMMENT         "HpaI" "I-SceI" "KasI" "KpnI" "MfeI" "MluI" "MscI" "MslI" "MspA1I" 
COMMENT         "NaeI" "NarI" "NcoI" "NdeI" "NgoMIV" "NheI" "NotI" "NruI" "NsiI" "NspI" 
COMMENT         "PacI" "PciI" "PflFI" "PflMI" "PI-SceI" "PmeI" "PmlI" "PpuMI" "PshAI" 
COMMENT         "PsiI" "PspOMI" "PstI" "PvuI" "PvuII" "RsrII" "SacI" "SacII" "SalI" 
COMMENT         "SapI" "SbfI" "ScaI" "SexAI" "SfcI" "SfiI" "SfoI" "SgrAI" "SmaI" "SmlI" 
COMMENT         "SnaBI" "SpeI" "SphI" "SrfI" "SspI" "StuI" "StyI" "SwaI" "Tth111I" 
COMMENT         "XbaI" "XcmI" "XhoI" "XmaI" "XmnI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 1 0 0 0 0 1 5657 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "AatI" "aggcct" 4 0 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AatII" "gacgtc" 6 0 3 459 0 1387 0 2548 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 1 1192 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "AccI" "gtmkac" 3 0 2 860 0 2694 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "AclI" "aacgtt" 3 0 2 3987 0 4360 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "Acu I" "ctgaag" 23 21 5 364 1 463 0 1670 0 
COMMENT            3923 0 4935 1 0 "") 
COMMENT        #6=(CRSite (CStringList) "AfeI" "agcgct" 4 0 1 604 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "AflII" "cttaag" 2 0 1 718 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "AflIII" "acrygt" 2 0 3 1479 0 1526 0 5477 0 0 
COMMENT            "") #9=(CRSite (CStringList) "AgeI" "accggt" 2 0 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "AhdI" "gacnnnnngtc" 7 0 3 59 0 3086 0 4589 0 
COMMENT             0 "") #11=(CRSite (CStringList) "AleI" "cacnnnngtg" 6 0 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AlwNI" "cagnnnctg" 7 0 1 5068 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 202 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 3 2304 0 3917 0 5163 0 0 
COMMENT             "") #15=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "AseI" "attaat" 3 0 2 4414 0 5649 0 0 "") 
COMMENT        #17=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 6 29 0 40 0 1584 0 2704 0 
COMMENT             3100 0 3111 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "BaeI" "acnnnngtayc" 24 19 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 681 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 6 252 0 588 0 1192 0 1315 
COMMENT             0 3405 0 4636 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "BanII" "grgcyc" 6 0 4 202 0 925 0 2448 0 3375 
COMMENT             0 0 "") 
COMMENT        #24=(CRSite (CStringList) "BbsI" "gaagac" 9 13 2 1502 0 2623 1 0 "") 
COMMENT        #25=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 1 435 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "BcgI" "cgannnnnntgc" 25 23 3 380 0 796 1 4051 
COMMENT             1 0 "") 
COMMENT        #27=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 3 2164 1 3752 0 5279 0 
COMMENT             0 "") 
COMMENT        #28=(CRSite (CStringList) "BclI" "tgatca" 2 0 3 1217 0 1787 0 2006 0 0 
COMMENT             "") #29=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BfuAI" "acctgc" 11 15 1 1554 1 0 "") 
COMMENT        #31=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 4 31 0 3116 0 3204 0 
COMMENT             4471 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BglII" "agatct" 2 0 1 185 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 2 919 0 924 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 2 871 0 1529 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "BmrI" "actggg" 12 11 1 4549 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "BpmI" "ctggag" 23 21 5 770 0 1123 0 1132 0 
COMMENT             1555 0 4520 0 0 "") 
COMMENT        #37=(CRSite (CStringList) "Bpu10I" "cctnagc" 3 6 1 435 0 0 "") 
COMMENT        #38=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 4 3985 0 4853 0 5094 1 
COMMENT             5392 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "BsaAI" "yacgtr" 4 0 5 167 0 1560 0 1956 0 
COMMENT             2977 0 3446 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "BsaBI" "gatnnnnatc" 6 0 2 842 0 2954 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 7 45 0 456 0 589 0 1384 0 
COMMENT             2545 0 3097 0 4049 0 0 "") 
COMMENT        #42=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 2 1751 0 4523 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "BsaXI" "acnnnnnctcc" 22 19 3 622 1 3485 1 
COMMENT             5639 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "BseRI" "gaggag" 17 15 5 646 0 767 0 1129 0 
COMMENT             2220 1 2281 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "BseYI" "cccagc" 2 6 4 524 0 951 0 1728 1 5173 
COMMENT             0 0 "") 
COMMENT        #46=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 2 1582 0 1958 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "BsiEI" "cgrycg" 5 0 7 268 0 2713 0 3175 0 
COMMENT             4071 0 4220 0 5143 0 5567 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "BsiHKAI" "gwgcwc" 6 0 5 925 0 2308 0 3921 0 
COMMENT             4006 0 5167 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 1 1535 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 2 2774 0 2867 1 0 "") 
COMMENT        #52=(CRSite (CStringList) "Bsp1286I" "gdgchc" 6 0 11 202 0 565 0 925 0 
COMMENT             1318 0 2308 0 2448 0 2461 0 3375 0 3921 0 4006 0 5167 0 0 "") 
COMMENT        #53=(CRSite (CStringList) "BspDI" "atcgat" 3 0 3 2090 0 2685 0 2948 0 0 
COMMENT             "") #54=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #55=(CRSite (CStringList) "BspHI" "tcatga" 2 0 2 3749 0 4757 0 0 "") 
COMMENT        #56=(CRSite (CStringList) "BspMI" "acctgc" 11 15 1 1554 1 0 "") 
COMMENT        #57=(CRSite (CStringList) "BsrBI" "gagcgg" 4 4 4 1043 1 3302 1 3747 1 
COMMENT             5548 1 0 "") 
COMMENT        #58=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 5 1596 0 1929 0 2342 1 
COMMENT             4355 0 4529 1 0 "") 
COMMENT        #59=(CRSite (CStringList) "BsrFI" "rccggy" 2 0 6 465 0 519 0 591 0 2607 
COMMENT             0 3341 0 4504 0 0 "") 
COMMENT        #60=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 1 2058 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 7 3 0 88 0 418 0 420 0 
COMMENT             448 0 3052 0 3137 0 0 "") 
COMMENT        #62=(CRSite (CStringList) "BssSI" "cacgag" 2 6 2 3920 0 5304 0 0 "") 
COMMENT        #63=(CRSite (CStringList) "BstAPI" "gcannnnntgc" 8 0 0 0 "") 
COMMENT        #64=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 1 1009 0 0 "") 
COMMENT        #66=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 2 1170 0 1728 0 0 
COMMENT             "") 
COMMENT        #67=(CRSite (CStringList) "BstYI" "rgatcy" 2 0 12 185 0 681 0 819 0 843 
COMMENT             0 2657 0 2955 0 3942 0 3959 0 4727 0 4739 0 4825 0 4836 0 0 "") 
COMMENT        #68=(CRSite (CStringList) "BstZ17I" "gtatac" 4 0 0 0 "") 
COMMENT        #69=(CRSite (CStringList) "Bsu36I" "cctnagg" 3 0 0 0 "") 
COMMENT        #70=(CRSite (CStringList) "BtgI" "ccrygg" 2 0 4 387 0 2399 0 2471 0 2595 
COMMENT             0 0 "") 
COMMENT        #71=(CRSite (CStringList) "BtgZ I" "gcgatg" 17 21 3 2039 0 2397 1 3441 1 
COMMENT             0 "") 
COMMENT        #72=(CRSite (CStringList) "BtsCI" "ggatgnn" 8 6 13 241 0 317 1 355 0 963 
COMMENT             0 1373 1 1430 1 1545 0 1710 0 1923 0 2037 0 4143 0 4430 0 4611 0 0 
COMMENT             "") 
COMMENT        #73=(CRSite (CStringList) "BtsI" "gcagtg" 9 7 4 369 1 2779 0 4170 0 4190 
COMMENT             1 0 "") #74=(CRSite (CStringList) "ClaI (m)" "gatcgat" 4 6 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "ClaI (nm)" "hatcgatd" 4 0 3 2090 0 2685 0 
COMMENT             2948 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "DraI" "tttaaa" 4 0 5 1671 0 2914 0 4011 0 
COMMENT             4703 0 4722 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "DraIII" "cacnnngtg" 7 0 1 3449 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "DrdI" "gacnnnnnngtc" 8 0 2 3493 0 5375 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "EaeI" "yggccr" 2 0 7 104 0 491 0 1266 0 2710 
COMMENT             0 3036 0 4196 0 5638 0 0 "") 
COMMENT        #80=(CRSite (CStringList) "EagI" "cggccg" 2 0 1 2710 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "EarI" "ctcttc" 8 11 7 1646 1 1985 1 2045 1 
COMMENT             2627 0 3153 1 3790 1 5594 1 0 "") 
COMMENT        #82=(CRSite (CStringList) "EciI" "ggcgga" 18 16 4 2419 0 4447 0 5275 0 
COMMENT             5421 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "EcoNI" "cctnnnnnagg" 6 0 1 1219 0 0 "") 
COMMENT        #84=(CRSite (CStringList) "EcoO109I" "rggnccy" 3 0 2 198 0 199 0 0 "") 
COMMENT        #85=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 662 0 0 "") 
COMMENT        #86=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "FseI" "ggccggcc" 7 0 0 0 "") 
COMMENT        #88=(CRSite (CStringList) "FspI" "tgcgca" 4 0 4 316 0 368 0 3194 0 4366 
COMMENT             0 0 "") 
COMMENT        #89=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 8 592 0 606 0 991 0 1110 
COMMENT             0 3291 0 3299 0 5237 0 5607 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "HincII" "gtyrac" 4 0 4 861 0 1982 0 2695 0 
COMMENT             2853 0 0 "") 
COMMENT        #91=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2083 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "HpaI" "gttaac" 4 0 2 1982 0 2853 0 0 "") 
COMMENT        #93=(CRSite (CStringList) "I-SceI" "twgggataacagggtaat" 10 6 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "KasI" "ggcgcc" 2 0 1 588 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 1196 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "MfeI" "caattg" 2 0 1 2862 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "MluI" "acgcgt" 2 0 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "MscI" "tggcca" 4 0 2 106 0 3038 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "MslI" "caynnnnrtg" 6 0 7 776 0 982 0 1069 0 
COMMENT             1234 0 3818 0 4177 0 4336 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "MspA1I" "cmgckg" 4 0 12 389 0 628 0 955 0 
COMMENT              1356 0 2432 0 2581 0 2597 0 3144 0 3953 0 4894 0 5139 0 5657 0 0 
COMMENT              "") 
COMMENT        #101=(CRSite (CStringList) "NaeI" "gccggc" 4 0 4 521 0 593 0 2609 0 3343 
COMMENT              0 0 "") 
COMMENT        #102=(CRSite (CStringList) "NarI" "ggcgcc" 3 0 1 589 0 0 "") 
COMMENT        #103=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 0 0 "") 
COMMENT        #104=(CRSite (CStringList) "NdeI" "catatg" 3 0 0 0 "") 
COMMENT        #105=(CRSite (CStringList) "NgoMIV" "gccggc" 2 0 4 519 0 591 0 2607 0 
COMMENT              3341 0 0 "") 
COMMENT        #106=(CRSite (CStringList) "NheI" "gctagc" 2 0 2 673 0 787 0 0 "") 
COMMENT        #107=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 2710 0 0 "") 
COMMENT        #108=(CRSite (CStringList) "NruI" "tcgcga" 4 0 0 0 "") 
COMMENT        #109=(CRSite (CStringList) "NsiI" "atgcat" 6 0 0 0 "") 
COMMENT        #110=(CRSite (CStringList) "NspI" "rcatgy" 6 0 2 1233 0 5481 0 0 "") 
COMMENT        #111=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 0 0 "") 
COMMENT        #112=(CRSite (CStringList) "PciI" "acatgt" 2 0 1 5477 0 0 "") 
COMMENT        #113=(CRSite (CStringList) "PflFI" "gacnnngtc" 5 0 0 0 "") 
COMMENT        #114=(CRSite (CStringList) "PflMI" "ccannnnntgg" 8 0 4 958 0 1101 0 1622 
COMMENT              0 2336 0 0 "") 
COMMENT        #115=(CRSite (CStringList) "PI-SceI" 
COMMENT              "atctatgtcgggtgcggagaaagaggtaatgaaatggca" 16 12 0 0 "") 
COMMENT        #116=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 0 0 "") 
COMMENT        #117=(CRSite (CStringList) "PmlI" "cacgtg" 4 0 2 1560 0 1956 0 0 "") 
COMMENT        #118=(CRSite (CStringList) "PpuMI" "rggwccy" 3 0 0 0 "") 
COMMENT        #119=(CRSite (CStringList) "PshAI" "gacnnnngtc" 6 0 0 0 "") 
COMMENT        #120=(CRSite (CStringList) "PsiI" "ttataa" 4 0 2 2833 0 3574 0 0 "") 
COMMENT        #121=(CRSite (CStringList) "PspOMI" "gggccc" 2 0 1 198 0 0 "") 
COMMENT        #122=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 195 0 704 0 1781 0 0 "") 
COMMENT        #123=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 3175 0 4220 0 0 "") 
COMMENT        #124=(CRSite (CStringList) "PvuII" "cagctg" 4 0 2 3144 0 5657 0 0 "") 
COMMENT        #125=(CRSite (CStringList) "RsrII" "cggwccg" 3 0 1 531 0 0 "") 
COMMENT        #126=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 925 0 0 "") 
COMMENT        #127=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 2 390 0 2598 0 0 "") 
COMMENT        #128=(CRSite (CStringList) "SalI" "gtcgac" 2 0 2 859 0 2693 0 0 "") 
COMMENT        #129=(CRSite (CStringList) "SapI" "gctcttc" 9 12 2 1646 1 5594 1 0 "") 
COMMENT        #130=(CRSite (CStringList) "SbfI" "cctgcagg" 7 0 0 0 "") 
COMMENT        #131=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 4108 0 0 "") 
COMMENT        #132=(CRSite (CStringList) "SexAI" "accwggt" 2 0 0 0 "") 
COMMENT        #133=(CRSite (CStringList) "SfcI" "ctryag" 2 0 8 191 0 700 0 908 0 1777 
COMMENT              0 3223 0 4343 0 5021 0 5212 0 0 "") 
COMMENT        #134=(CRSite (CStringList) "SfiI" "ggccnnnnnggcc" 9 0 0 0 "") 
COMMENT        #135=(CRSite (CStringList) "SfoI" "ggcgcc" 4 0 1 590 0 0 "") 
COMMENT        #136=(CRSite (CStringList) "SgrAI" "crccggyg" 3 0 1 591 0 0 "") 
COMMENT        #137=(CRSite (CStringList) "SmaI" "cccggg" 4 0 5 31 0 42 0 2706 0 3102 0 
COMMENT              3113 0 0 "") 
COMMENT        #138=(CRSite (CStringList) "SmlI" "ctyrag" 2 0 5 718 0 3964 0 4832 0 
COMMENT              5109 0 5371 0 0 "") 
COMMENT        #139=(CRSite (CStringList) "SnaBI" "tacgta" 4 0 2 167 0 2977 0 0 "") 
COMMENT        #140=(CRSite (CStringList) "SpeI" "actagt" 2 0 0 0 "") 
COMMENT        #141=(CRSite (CStringList) "SphI" "gcatgc" 6 0 0 0 "") 
COMMENT        #142=(CRSite (CStringList) "SrfI" "gcccgggc" 5 0 4 31 0 42 0 3102 0 3113 
COMMENT              0 0 "") 
COMMENT        #143=(CRSite (CStringList) "SspI" "aatatt" 4 0 2 3654 0 3784 0 0 "") 
COMMENT        #144=(CRSite (CStringList) "StuI" "aggcct" 4 0 0 0 "") 
COMMENT        #145=(CRSite (CStringList) "StyI" "ccwwgg" 2 0 4 497 0 1721 0 1968 0 
COMMENT              2503 0 0 "") 
COMMENT        #146=(CRSite (CStringList) "SwaI" "atttaaat" 5 0 0 0 "") 
COMMENT        #147=(CRSite (CStringList) "Tth111I" "gacnnngtc" 5 0 0 0 "") 
COMMENT        #148=(CRSite (CStringList) "XbaI" "tctaga" 2 0 0 0 "") 
COMMENT        #149=(CRSite (CStringList) "XcmI" "ccannnnnnnnntgg" 9 0 0 0 "") 
COMMENT        #150=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 0 0 "") 
COMMENT        #151=(CRSite (CStringList) "XmaI" "cccggg" 2 0 5 29 0 40 0 2704 0 3100 0 
COMMENT              3111 0 0 "") 
COMMENT        #152=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 1 3989 0 0 "")) 
COMMENT       (CObList 
COMMENT        #153=(CFSignal (CObList) "Amp" 21 0 0 3801 4658 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "3857..4714") 
COMMENT        #154=(CFSignal (CObList) "5' ITR" 21 0 0 1 167 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "57..223") 
COMMENT        #155=(CFSignal (CObList) "3' ITR" 21 0 0 2976 3142 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "3032..3198") 
COMMENT        #156=(CFSignal (CObList) "NES-jRCaMP1a" 21 0 0 696 2081 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "<752..2137") 
COMMENT        #157=(CFSignal (CObList) "SV40 polyadenylation signal" 25 0 0 2716 2947 
COMMENT              0 (CStringList) (CStringList) 1 1 1 1 "2772..3003") 
COMMENT        #158=(CFSignal (CObList) "synapsin promoter" 29 0 0 190 661 0 
COMMENT              (CStringList) (CStringList) 1 1 1 1 "246..717") 
COMMENT        #159=(CFSignal (CObList) "WPRE" 21 0 0 2094 2682 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "2150..2738")) (CObList) (CObList) (CObList) 
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COMMENT                             "DNA 'pAAV.Syn.NES.jRCaMP1a.WPRE.SV40(p3855)'" 1) 
COMMENT                       #164=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Foreign object. Author: Andres Fernandez. Original author: Andres Fernandez" 
COMMENT                             1) 
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COMMENT                             "GAAGACNNNNNNN" "CTTCTGNNNNNNN") 
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COMMENT                             "CGANNNNNNTGCNNNNNNNNNNNNN" 
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COMMENT                              "BciVI: 3 sites") 12 11 "GTATCCNNNNNNN" 
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COMMENT                              "BclI: 3 sites") 1 5 "TGATCA" "ACTAGT") 
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COMMENT                             "ACCTGCNNNNNNNNN" "TGGACGNNNNNNNNN") 
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COMMENT                              "BglI: 4 sites") 7 4 "GCCNNNNNGGC" "CGGNNNNNCCG") 
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COMMENT                              "BglII: 1 site") 1 5 "AGATCT" "TCTAGA") 
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COMMENT                              "BlpI: 2 sites") 2 5 "GCTNAGC" "CGANTCG") 
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COMMENT                              "BmgBI: 2 sites") 3 3 "CACGTC" "GTGCAG") 
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COMMENT                              "BmrI: 1 site") 11 10 "ACTGGGNNNNNN" 
COMMENT                             "TGACCCNNNNNN") 
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COMMENT                              "BpmI: 5 sites") 22 20 "CTGGAGNNNNNNNNNNNNNNNNN" 
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COMMENT                              "Bpu10I: 1 site") 2 5 "CCTNAGC" "GGANTCG") 
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COMMENT                              "BpuEI: 4 sites") 22 20 "CTTGAGNNNNNNNNNNNNNNNNN" 
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COMMENT                                       #371=(CLinePar 
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COMMENT                              "BsaAI: 5 sites") 3 3 "YACGTR" "RTGCAY") 
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COMMENT                              "BsaBI: 2 sites") 5 5 "GATNNNNATC" "CTANNNNTAG") 
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COMMENT                                       #382=(CLinePar 
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COMMENT                                       #383=(CLinePar 
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COMMENT                              "BsaHI: 7 sites") 2 4 "GRCGYC" "CYGCRG") 
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COMMENT                              "BsaI: 2 sites") 7 11 "GGTCTCNNNNNN" 
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COMMENT                              "BsaXI: 3 sites") 21 18 "ACNNNNNCTCCNNNNNNNNNNN" 
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COMMENT                                       #401=(CLinePar 
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COMMENT                              "BseRI: 5 sites") 16 14 "GAGGAGNNNNNNNNNNN" 
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COMMENT                                       #407=(CLinePar 
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COMMENT                                             " N3: 1728 (on complementary strand)" 
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COMMENT                              "BseYI: 4 sites") 1 5 "CCCAGC" "GGGTCG") 
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COMMENT                              "BsgI: 2 sites") 22 20 "GTGCAGNNNNNNNNNNNNNNNNN" 
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COMMENT                                       #416=(CLinePar 
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COMMENT                                       #420=(CLinePar 
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COMMENT                                       #421=(CLinePar 
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COMMENT                              "BsiEI: 7 sites") 4 2 "CGRYCG" "GCYRGC") 
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COMMENT                              "BsiHKAI: 5 sites") 5 1 "GWGCWC" "CWCGWG") 
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COMMENT                              "BsmBI: 1 site") 7 11 "CGTCTCNNNNNN" 
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COMMENT                             "GAATGCNN" "CTTACGNN") 
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COMMENT                                       #443=(CLinePar 
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COMMENT                             "GCGATGNNNNNNNNNNNNNNN" "CGCTACNNNNNNNNNNNNNNN") 
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COMMENT                                       #556=(CLinePar 
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COMMENT                              "ClaI (nm): 3 sites") 3 5 "HATCGATD" "DTAGCTAH") 
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COMMENT                              "DraIII: 1 site") 6 3 "CACNNNGTG" "GTGNNNCAC") 
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COMMENT                              "DrdI: 2 sites") 7 5 "GACNNNNNNGTC" "CTGNNNNNNCAG") 
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FEATURES             Location/Qualifiers
     misc_feature    3801..4658
                     /vntifkey="21"
                     /label=Amp
                     /note="Amp"
     misc_feature    1..167
                     /vntifkey="21"
                     /label=5'\ITR
                     /note="5' ITR"
     misc_feature    2976..3142
                     /vntifkey="21"
                     /label=3'\ITR
                     /note="5' ITR"
     misc_feature    696..2081
                     /vntifkey="21"
                     /label=NES-jRCaMP1a
                     /note="NES-jRCaMP1a"
     polyA_signal    2716..2947
                     /vntifkey="25"
                     /label=SV40\polyadenylation\signal
                     /note="SV40 polyadenylation signal"
     promoter        190..661
                     /vntifkey="29"
                     /label=synapsin\promoter
                     /note="synapsin promoter"
     misc_feature    2094..2682
                     /vntifkey="21"
                     /label=WPRE
                     /note="WPRE"
BASE COUNT     1324 a      1464 c      1521 g      1348 t 
ORIGIN
        1 tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc cgggcgtcgg gcgacctttg 
       61 gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta 
      121 ggggttcctt gtagttaatg attaacccgc catgctactt atctacgtag ccatgctcta 
      181 ggaagatctc tgcagagggc cctgcgtatg agtgcaagtg ggttttagga ccaggatgag 
      241 gcggggtggg ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc 
      301 cattccccaa attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg 
      361 cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc 
      421 gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac 
      481 tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca 
      541 cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc 
      601 agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc 
      661 gaattcaagc tgctagcaag gatccacccg ccaccatgct gcagaacgag cttgctctta 
      721 agttggctgg acttgatatt aacaagactg gaggaggttc tcatcatcat catcatcatg 
      781 gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata 
      841 aggatctcgc aacaatggtc gactcatcac gtcgtaagtg gaataagaca ggtcacgcag 
      901 tcagagctat aggtcggctg agctcagcga tcaactgcga aatgatgtac ccagcggatg 
      961 gtggtctgcg tggttacact cacatggcgc tgaaagttga tggcggcggt cacctgtcct 
     1021 gttctttcgt gaccacctac cgctccaaaa agactgtcgg caacattaag atgcctgcca 
     1081 ttcatagcgt cagccaccgt ctggagcgcc tggaggagag cgataacgaa atgtttgtcg 
     1141 tacagcgtga acacgcagtt gccaagtttg tgggcctggg tggtggcggc ggtaccggag 
     1201 ggagcatgaa ctccctgatc aaggagaaca tgcgtatgaa agtggttctg gaaggctccg 
     1261 taaacggcca ccagttcaaa tgcactggtg aaggcgaagg caacccgtat atgggcaccc 
     1321 agactatgcg tatcaaagtg atcgagggtg gtccgctgcc gtttgcgttc gacatcctgg 
     1381 cgacgtcctt tatgtatggc tcccgtacct tcatcaaata tccgaaaggc atcccggatt 
     1441 tctttaagca gtccttcccg gaaggtttta cctgggaacg tgtgacccgt tacgaagacg 
     1501 gcggcgtaat taccgttatg caagacacgt ctctggagga tggctgcctg gtgtatcacg 
     1561 tgcaggttcg cggtgtgaac ttcccgagca atggtgctgt aatgcaaaag aaaaccaaag 
     1621 gttgggagcc tacggactcc caaaccactg aagagcagat cgcagaattt aaagaggctt 
     1681 tctccctatt tgacaaggac ggggatggga caataacaac caaggagctg gggacggtga 
     1741 tgcggtctct ggggcagaac cccacagaag cagagctgca ggacatgatc aatgaagtag 
     1801 atgccgacgg tgacggcaca atcgacttcc ctgagttcct gattatgatg gcaagaaaaa 
     1861 tgaaatacac agacagtgaa gaagaaatta gagaagcgtt cggcgtgttt gataaggatg 
     1921 gcaatggcta catcagtgca gcagagcttc gccacgtgat gacaaacctt ggagagaagt 
     1981 taacagatga agaggttgat gaaatgatca gggaagcaga cagcgatggg gatggtcagg 
     2041 taaactacga agagtttgta caaatgatga cagcgaagtg aaagcttatc gataatcaac 
     2101 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 
     2161 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 
     2221 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 
     2281 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 
     2341 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 
     2401 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 
     2461 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg 
     2521 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 
     2581 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc 
     2641 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc 
     2701 gacccgggcg gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca 
     2761 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat 
     2821 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt 
     2881 ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg 
     2941 gtaaaatcga taaggatctt cctagagcat ggctacgtag ataagtagca tggcgggtta 
     3001 atcattaact acaaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc 
     3061 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc 
     3121 tcagtgagcg agcgagcgcg cagctggcgt aatagcgaag aggcccgcac cgatcgccct 
     3181 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc 
     3241 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 
     3301 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 
     3361 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 
     3421 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 
     3481 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 
     3541 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 
     3601 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg 
     3661 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 
     3721 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 
     3781 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 
     3841 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 
     3901 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 
     3961 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 
     4021 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 
     4081 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 
     4141 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 
     4201 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 
     4261 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 
     4321 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 
     4381 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 
     4441 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 
     4501 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 
     4561 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 
     4621 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 
     4681 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 
     4741 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 
     4801 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 
     4861 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 
     4921 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 
     4981 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 
     5041 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 
     5101 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 
     5161 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 
     5221 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 
     5281 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 
     5341 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 
     5401 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 
     5461 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 
     5521 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 
     5581 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 
     5641 cgattcatta atgcagc 
//
