LOCUS       pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40(p3850)        6412 bp    DNA     circular      2-JUL-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|738431629|
COMMENT     LSOWNER|
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FEATURES             Location/Qualifiers
     misc_feature    complement(4898..5755)
                     /vntifkey="21"
                     /label=Amp
                     /note="Amp"
     promoter        193..1126
                     /vntifkey="29"
                     /label=CAG\promoter
                     /note="CAG promoter"
     misc_feature    1..168
                     /vntifkey="21"
                     /label=5'ITR
                     /note="5'ITR"
     misc_feature    3759..3925
                     /vntifkey="21"
                     /label=3'ITR
                     /note="3'ITR"
     misc_feature    1231..1264
                     /vntifkey="21"
                     /label=loxP
                     /note="loxP"
     misc_feature    2808..2841
                     /vntifkey="21"
                     /label=loxP
                     /note="loxP"
     misc_feature    complement(1135..1168)
                     /vntifkey="21"
                     /label=loxP2272
                     /note="loxP2272"
     misc_feature    2704..2737
                     /vntifkey="21"
                     /label=loxP2272
                     /note="loxP2272"
     misc_feature    complement(1275..2687)
                     /vntifkey="21"
                     /label=NES-jRCaMP1b
                     /note="NES-jRCaMP1b"
     polyA_signal    3499..3731
                     /vntifkey="25"
                     /label=SV40\polyadenylation\signal
                     /note="SV40 polyadenylation signal"
     misc_feature    2883..3471
                     /vntifkey="21"
                     /label=WPRE
                     /note="WPRE"
     polyA_signal    3472..3704
                     /vntifkey="25"
                     /label=SV40\polyadenylation\signal
                     /note="SV40 polyadenylation signal"
     misc_feature    2856..3444
                     /vntifkey="21"
                     /label=WPRE
                     /note="WPRE"
     misc_feature    731..731
                     /vntifkey="21"
                     /label=Deletion\-1bp\(A)
BASE COUNT     1438 a      1725 c      1608 g      1641 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagagta ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 
      241 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 
      301 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 
      361 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt 
      421 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc 
      481 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat 
      541 gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg 
      601 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc 
      661 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg 
      721 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc 
      781 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 
      841 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag 
      901 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggc tgtccgcggg 
      961 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg 
     1021 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg 
     1081 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattggat ccccataact 
     1141 tcgtataaag tatcctatac gaagttatat caaaatagga agaccaatgc ttcaccatcg 
     1201 acccgaattg ccaagcatca ccatcgaccc ataacttcgt ataatgtatg ctatacgaag 
     1261 ttatactaga gctttcactt cgctgtcatc atttgtacaa actcttcgta gtttacctga 
     1321 ccatccccat cgctgtctgc ttccctgatc atttcatcaa cctcttcatc tgttaacttc 
     1381 tctccaaggt ttgtcatcac gtggcgaagc tctgctgcac tgatgtagcc attgccatcc 
     1441 ttatcaaaca cgccgaacgc ttctctaatt tcttcttcac tgtctgtgta tttcattttg 
     1501 cctgccatca taatcaggaa ctcagggaag tcgattgtgc cgtcaccgtc ggcatctact 
     1561 tcattgatca tgtcctgcag ctctgcttct gtggggttct gccccagaga ccgcatcacc 
     1621 gtccccatct ccttggttgt tattgtccca tccccgtcct tgtcaaatag ggagaaagcc 
     1681 tctttaaatt ctgcgatctg ctcttcagtc agttgggagt ccgtaggctc ccaacctttg 
     1741 gttttctttt gcattacagc accattgctc gggaagttca caccgcgaac ctgcacgtga 
     1801 tacaccaggc agccatcctc cagagacgtg tcttgcataa cggtaattac gccgccgtct 
     1861 tcgtaacggg tcacacgttc ccaggtaaaa ccttccggga aggactgctt aaagaaatcc 
     1921 gggatgcctt tcggatattt gatgaaggta cgggagccat acataaagga cgtcgccagg 
     1981 atgtcgaacg caaacggcag cggaccaccc tcgatcactt tgatacgcat agtctgggtg 
     2041 cccatatacg ggttgccttc gccttcacca gtgcatttga actggtggcc gtttacggag 
     2101 ccttccagaa ccactttcat acgcatgttc tccttgatca gggagttcat gctccctccg 
     2161 gtaccgccgc caccacccag gcccacaaac ttggcaactg cgtgttcacg ctgtacgaca 
     2221 aacatttcgt tatcgctctc ctccaggcgc tccagacggt ggctgacgta atgaatggca 
     2281 ggcatcttaa tgttgccgac agtctttttg gagcggtagg tggtcacgaa agaacaggac 
     2341 aggtgaccgc cgccatcaac tttcagcgcc atgtgagtgt aaccacgcag accaccatcc 
     2401 gctgggtaca tcatttcggt gttgttcgct gagctcagcc gacctatagc tctgactgcg 
     2461 tgaccccact tattccactt acgtcgcgat gagtcgacca ttgttgcgag atccttatcg 
     2521 tcatcgtcgt acagatcccg acccatttgc tgtccaccag tcatgctagc cataccatga 
     2581 tgatgatgat gatgagaacc tcctccagtc ttgttaatat caagtccagc caacttaaga 
     2641 gcaagctcgt tctgcagcat ggtggcggat cctagtataa cttcgtatag gatactttat 
     2701 acgaagttat cattgggatt cttcctattt tgatccaagc atcaccatcg accctctagt 
     2761 ccagatctca ccatcgaccc ataacttcgt atagcataca ttatacgaag ttatgtccct 
     2821 cgaagaggtt cgaattcgat atcaagctta tcgataatca acctctggat tacaaaattt 
     2881 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg 
     2941 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt 
     3001 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 
     3061 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 
     3121 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg 
     3181 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt 
     3241 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc 
     3301 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg 
     3361 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga 
     3421 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc cgggcggccg cttcgagcag 
     3481 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat 
     3541 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata 
     3601 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg 
     3661 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatcttccta 
     3721 gagcatggct acgtagataa gtagcatggc gggttaatca ttaactacaa ggaaccccta 
     3781 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca 
     3841 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc 
     3901 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 
     3961 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 
     4021 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 
     4081 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 
     4141 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 
     4201 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 
     4261 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 
     4321 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 
     4381 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 
     4441 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 
     4501 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 
     4561 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 
     4621 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 
     4681 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 
     4741 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 
     4801 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 
     4861 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 
     4921 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 
     4981 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 
     5041 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 
     5101 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 
     5161 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 
     5221 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 
     5281 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 
     5341 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 
     5401 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 
     5461 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 
     5521 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 
     5581 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 
     5641 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 
     5701 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 
     5761 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 
     5821 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 
     5881 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 
     5941 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 
     6001 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 
     6061 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 
     6121 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 
     6181 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 
     6241 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 
     6301 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct 
     6361 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc ag 
//
