LOCUS       pAAV.CAG.Flex.NES-jRGECO1b.WPRE.SV40(p3844)        6439 bp    DNA     circular     18-JUN-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|737028593|
COMMENT     VNTDBDATE|737034515|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV.CAG.Flex.NES-jRGECO1b.WPRE.SV40(p3844)|
COMMENT     VNTAUTHORNAME|Erich Kurtz|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAAV.CAG.Flex.NES-jRGECO1b.WPRE.SV40(p3844)" 0 6439 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "AatI" "AatII" "Acc65I" "AccI" "AclI" "Acu I" "AfeI" 
COMMENT         "AflII" "AflIII" "AgeI" "AhdI" "AleI" "AlwNI" "ApaI" "ApaLI" "AscI" 
COMMENT         "AseI" "AsiSI" "AvaI" "AvrII" "BaeI" "BamHI" "BanI" "BanII" "BbsI" 
COMMENT         "BbvCI" "BcgI" "BciVI" "BclI" "BfrBI" "BfuAI" "BglI" "BglII" "BlpI" 
COMMENT         "BmgBI" "BmrI" "BpmI" "Bpu10I" "BpuEI" "BsaAI" "BsaBI" "BsaHI" "BsaI" 
COMMENT         "BsaXI" "BseRI" "BseYI" "BsgI" "BsiEI" "BsiHKAI" "BsiWI" "BsmBI" "BsmI" 
COMMENT         "Bsp1286I" "BspDI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrFI" 
COMMENT         "BsrGI" "BssHII" "BssSI" "BstAPI" "BstBI" "BstEII" "BstXI" "BstYI" 
COMMENT         "BstZ17I" "Bsu36I" "BtgI" "BtgZ I" "BtsCI" "BtsI" "ClaI (m)" 
COMMENT         "ClaI (nm)" "DraI" "DraIII" "DrdI" "EaeI" "EagI" "EarI" "EciI" "EcoNI" 
COMMENT         "EcoO109I" "EcoRI" "EcoRV" "FseI" "FspI" "HaeII" "HincII" "HindIII" 
COMMENT         "HpaI" "I-SceI" "KasI" "KpnI" "MfeI" "MluI" "MscI" "MslI" "MspA1I" 
COMMENT         "NaeI" "NarI" "NcoI" "NdeI" "NgoMIV" "NheI" "NotI" "NruI" "NsiI" "NspI" 
COMMENT         "PacI" "PciI" "PflFI" "PflMI" "PI-SceI" "PmeI" "PmlI" "PpuMI" "PshAI" 
COMMENT         "PsiI" "PspOMI" "PstI" "PvuI" "PvuII" "RsrII" "SacI" "SacII" "SalI" 
COMMENT         "SapI" "SbfI" "ScaI" "SexAI" "SfcI" "SfiI" "SfoI" "SgrAI" "SmaI" "SmlI" 
COMMENT         "SnaBI" "SpeI" "SphI" "SrfI" "SspI" "StuI" "StyI" "SwaI" "Tth111I" 
COMMENT         "XbaI" "XcmI" "XhoI" "XmaI" "XmnI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 6439 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "AatI" "aggcct" 4 0 2 1949 0 2042 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AatII" "gacgtc" 6 0 4 201 0 254 0 337 0 3337 0 
COMMENT            0 "") #2=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "AccI" "gtmkac" 3 0 2 2522 0 3483 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "AclI" "aacgtt" 3 0 2 5222 0 5595 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "Acu I" "ctgaag" 23 21 6 1689 1 1907 0 2005 1 
COMMENT            2368 1 4651 0 5663 1 0 "") 
COMMENT        #6=(CRSite (CStringList) "AfeI" "agcgct" 4 0 1 855 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "AflII" "cttaag" 2 0 2 2413 0 2662 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "AflIII" "acrygt" 2 0 2 1720 0 4103 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "AgeI" "accggt" 2 0 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "AhdI" "gacnnnnngtc" 7 0 4 60 0 1981 0 3869 0 
COMMENT             4996 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "AleI" "cacnnnngtg" 6 0 1 2258 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AlwNI" "cagnnnctg" 7 0 2 1077 0 4519 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 924 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 4 2110 0 3093 0 4417 0 
COMMENT             5663 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "AseI" "attaat" 3 0 2 3933 0 5168 0 0 "") 
COMMENT        #17=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 1 1612 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 8 30 0 41 0 944 0 1894 0 
COMMENT             2428 0 3487 0 3883 0 3894 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "BaeI" "acnnnngtayc" 24 19 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 1128 0 2695 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 5 1780 0 2311 0 2419 0 
COMMENT             4944 0 6175 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "BanII" "grgcyc" 6 0 7 453 0 913 0 924 0 2103 
COMMENT             0 2463 0 3237 0 6213 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "BbsI" "gaagac" 9 13 3 1187 0 1735 1 3412 1 0 
COMMENT             "") #25=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 1 2390 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "BcgI" "cgannnnnntgc" 25 23 4 1205 1 2590 0 
COMMENT             2756 1 5535 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 5 1162 0 2712 1 2953 1 
COMMENT             4306 1 5833 1 0 "") 
COMMENT        #28=(CRSite (CStringList) "BclI" "tgatca" 2 0 2 1347 0 1566 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BfuAI" "acctgc" 11 15 1 2500 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 6 32 0 288 0 359 0 
COMMENT             3899 0 5116 0 6383 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BglII" "agatct" 2 0 1 2791 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 2 2026 0 2462 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 1 2513 1 0 "") 
COMMENT        #35=(CRSite (CStringList) "BmrI" "actggg" 12 11 2 365 1 5036 1 0 "") 
COMMENT        #36=(CRSite (CStringList) "BpmI" "ctggag" 23 21 2 2616 1 5066 1 0 "") 
COMMENT        #37=(CRSite (CStringList) "Bpu10I" "cctnagc" 3 6 1 2390 0 0 "") 
COMMENT        #38=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 5 924 0 4194 1 4492 0 
COMMENT             4733 1 5601 1 0 "") 
COMMENT        #39=(CRSite (CStringList) "BsaAI" "yacgtr" 4 0 6 168 0 416 0 1401 0 1842 
COMMENT             0 3760 0 6138 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "BsaBI" "gatnnnnatc" 6 0 2 2542 0 3737 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 9 46 0 198 0 251 0 334 0 
COMMENT             2312 0 2420 0 3334 0 3880 0 5533 0 0 "") 
COMMENT        #42=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 1 5057 1 0 "") 
COMMENT        #43=(CRSite (CStringList) "BsaXI" "acnnnnnctcc" 22 19 4 1820 1 2270 1 
COMMENT             3948 1 6102 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "BseRI" "gaggag" 17 15 7 826 1 1354 1 1841 0 
COMMENT             2146 1 2619 1 3009 1 3070 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "BseYI" "cccagc" 2 6 4 1604 0 1731 0 1931 1 
COMMENT             4407 1 0 "") 
COMMENT        #46=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 2 553 0 2308 1 0 "") 
COMMENT        #47=(CRSite (CStringList) "BsiEI" "cgrycg" 5 0 7 1612 0 3496 0 4019 0 
COMMENT             4443 0 5366 0 5515 0 6411 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "BsiHKAI" "gwgcwc" 6 0 6 2114 0 2463 0 3097 0 
COMMENT             4421 0 5582 0 5667 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 1 2273 1 0 "") 
COMMENT        #51=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 2 3557 0 3650 1 0 "") 
COMMENT        #52=(CRSite (CStringList) "Bsp1286I" "gdgchc" 6 0 14 453 0 751 0 913 0 
COMMENT             924 0 2103 0 2114 0 2463 0 3097 0 3237 0 3250 0 4421 0 5582 0 5667 
COMMENT             0 6213 0 0 "") 
COMMENT        #53=(CRSite (CStringList) "BspDI" "atcgat" 3 0 4 1331 0 2879 0 3474 0 
COMMENT             3731 0 0 "") 
COMMENT        #54=(CRSite (CStringList) "BspEI" "tccgga" 2 0 1 1766 0 0 "") 
COMMENT        #55=(CRSite (CStringList) "BspHI" "tcatga" 2 0 2 4823 0 5831 0 0 "") 
COMMENT        #56=(CRSite (CStringList) "BspMI" "acctgc" 11 15 1 2500 0 0 "") 
COMMENT        #57=(CRSite (CStringList) "BsrBI" "gagcgg" 4 4 8 640 0 754 1 817 0 940 0 
COMMENT             2442 1 4036 0 5837 0 6282 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 4 1430 1 3131 1 5057 0 
COMMENT             5231 1 0 "") 
COMMENT        #59=(CRSite (CStringList) "BsrFI" "rccggy" 2 0 5 1018 0 2215 0 3396 0 
COMMENT             5076 0 6239 0 0 "") 
COMMENT        #60=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 2 1295 0 2196 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 9 4 0 89 0 563 0 565 0 
COMMENT             643 0 706 0 729 0 3835 0 3920 0 0 "") 
COMMENT        #62=(CRSite (CStringList) "BssSI" "cacgag" 2 6 3 2083 1 4276 1 5660 1 0 
COMMENT             "") #63=(CRSite (CStringList) "BstAPI" "gcannnnntgc" 8 0 0 0 "") 
COMMENT        #64=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 1 2858 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 1 2014 0 0 "") 
COMMENT        #66=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 0 0 "") 
COMMENT        #67=(CRSite (CStringList) "BstYI" "rgatcy" 2 0 13 1128 0 2537 0 2561 0 
COMMENT             2695 0 2791 0 3446 0 3738 0 4744 0 4755 0 4841 0 4853 0 5621 0 5638 
COMMENT             0 0 "") #68=(CRSite (CStringList) "BstZ17I" "gtatac" 4 0 0 0 "") 
COMMENT        #69=(CRSite (CStringList) "Bsu36I" "cctnagg" 3 0 0 0 "") 
COMMENT        #70=(CRSite (CStringList) "BtgI" "ccrygg" 2 0 7 436 0 955 0 1737 0 2451 
COMMENT             0 3188 0 3260 0 3384 0 0 "") 
COMMENT        #71=(CRSite (CStringList) "BtgZ I" "gcgatg" 17 21 4 410 1 552 0 3186 1 
COMMENT             6139 0 0 "") 
COMMENT        #72=(CRSite (CStringList) "BtsCI" "ggatgnn" 8 6 8 1322 1 1649 1 1929 0 
COMMENT             1986 0 2436 1 4975 1 5156 1 5443 1 0 "") 
COMMENT        #73=(CRSite (CStringList) "BtsI" "gcagtg" 9 7 3 3562 0 5396 0 5416 1 0 
COMMENT             "") #74=(CRSite (CStringList) "ClaI (m)" "gatcgat" 4 6 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "ClaI (nm)" "hatcgatd" 4 0 4 1331 0 2879 0 
COMMENT             3474 0 3731 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "DraI" "tttaaa" 4 0 5 1686 0 3697 0 4862 0 
COMMENT             4881 0 5573 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "DraIII" "cacnnngtg" 7 0 1 6138 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "DrdI" "gacnnnnnngtc" 8 0 3 2784 0 4211 0 6093 
COMMENT             0 0 "") 
COMMENT        #79=(CRSite (CStringList) "EaeI" "yggccr" 2 0 8 105 0 2087 0 2142 0 2372 
COMMENT             0 3493 0 3819 0 3942 0 5384 0 0 "") 
COMMENT        #80=(CRSite (CStringList) "EagI" "cggccg" 2 0 1 3493 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "EarI" "ctcttc" 8 11 9 179 1 1309 0 1480 0 
COMMENT             1708 0 2845 1 3416 0 3987 0 5791 0 6428 0 0 "") 
COMMENT        #82=(CRSite (CStringList) "EciI" "ggcgga" 18 16 7 626 0 746 1 2708 0 
COMMENT             3208 0 4165 1 4311 1 5139 1 0 "") 
COMMENT        #83=(CRSite (CStringList) "EcoNI" "cctnnnnnagg" 6 0 1 2850 0 0 "") 
COMMENT        #84=(CRSite (CStringList) "EcoO109I" "rggnccy" 3 0 2 920 0 921 0 0 "") 
COMMENT        #85=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2860 0 0 "") 
COMMENT        #86=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 2868 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "FseI" "ggccggcc" 7 0 0 0 "") 
COMMENT        #88=(CRSite (CStringList) "FspI" "tgcgca" 4 0 2 5218 0 6390 0 0 "") 
COMMENT        #89=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 7 857 0 2315 0 2423 0 
COMMENT             3981 0 4351 0 6289 0 6297 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "HincII" "gtyrac" 4 0 4 1375 0 2523 0 3484 0 
COMMENT             3636 0 0 "") 
COMMENT        #91=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2872 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "HpaI" "gttaac" 4 0 2 1375 0 3636 0 0 "") 
COMMENT        #93=(CRSite (CStringList) "I-SceI" "twgggataacagggtaat" 10 6 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "KasI" "ggcgcc" 2 0 2 2311 0 2419 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "MfeI" "caattg" 2 0 1 3645 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 1720 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "MscI" "tggcca" 4 0 3 107 0 2144 0 3821 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "MslI" "caynnnnrtg" 6 0 6 1049 0 2258 0 2608 0 
COMMENT             5248 0 5407 0 5766 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "MspA1I" "cmgckg" 4 0 10 1 0 957 0 2322 0 
COMMENT              3221 0 3370 0 3386 0 3927 0 4445 0 4690 0 5631 0 0 "") 
COMMENT        #101=(CRSite (CStringList) "NaeI" "gccggc" 4 0 2 3398 0 6241 0 0 "") 
COMMENT        #102=(CRSite (CStringList) "NarI" "ggcgcc" 3 0 2 2312 0 2420 0 0 "") 
COMMENT        #103=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 436 0 1737 0 0 "") 
COMMENT        #104=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 310 0 0 "") 
COMMENT        #105=(CRSite (CStringList) "NgoMIV" "gccggc" 2 0 2 3396 0 6239 0 0 "") 
COMMENT        #106=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 2593 0 0 "") 
COMMENT        #107=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 3493 0 0 "") 
COMMENT        #108=(CRSite (CStringList) "NruI" "tcgcga" 4 0 1 1465 0 0 "") 
COMMENT        #109=(CRSite (CStringList) "NsiI" "atgcat" 6 0 0 0 "") 
COMMENT        #110=(CRSite (CStringList) "NspI" "rcatgy" 6 0 1 4107 0 0 "") 
COMMENT        #111=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 0 0 "") 
COMMENT        #112=(CRSite (CStringList) "PciI" "acatgt" 2 0 1 4103 0 0 "") 
COMMENT        #113=(CRSite (CStringList) "PflFI" "gacnnngtc" 5 0 1 2284 0 0 "") 
COMMENT        #114=(CRSite (CStringList) "PflMI" "ccannnnntgg" 8 0 2 1738 0 3125 0 0 
COMMENT              "") 
COMMENT        #115=(CRSite (CStringList) "PI-SceI" 
COMMENT              "atctatgtcgggtgcggagaaagaggtaatgaaatggca" 16 12 0 0 "") 
COMMENT        #116=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 0 0 "") 
COMMENT        #117=(CRSite (CStringList) "PmlI" "cacgtg" 4 0 2 1401 0 1842 0 0 "") 
COMMENT        #118=(CRSite (CStringList) "PpuMI" "rggwccy" 3 0 0 0 "") 
COMMENT        #119=(CRSite (CStringList) "PshAI" "gacnnnngtc" 6 0 0 0 "") 
COMMENT        #120=(CRSite (CStringList) "PsiI" "ttataa" 4 0 2 3616 0 6010 0 0 "") 
COMMENT        #121=(CRSite (CStringList) "PspOMI" "gggccc" 2 0 1 920 0 0 "") 
COMMENT        #122=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 1580 0 1823 0 2684 0 0 
COMMENT              "") 
COMMENT        #123=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 3 1612 0 5366 0 6411 0 0 
COMMENT              "") 
COMMENT        #124=(CRSite (CStringList) "PvuII" "cagctg" 4 0 3 1 0 2322 0 3927 0 0 "") 
COMMENT        #125=(CRSite (CStringList) "RsrII" "cggwccg" 3 0 0 0 "") 
COMMENT        #126=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 2463 0 0 "") 
COMMENT        #127=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 2 958 0 3387 0 0 "") 
COMMENT        #128=(CRSite (CStringList) "SalI" "gtcgac" 2 0 2 2521 0 3482 0 0 "") 
COMMENT        #129=(CRSite (CStringList) "SapI" "gctcttc" 9 12 2 1708 0 3987 0 0 "") 
COMMENT        #130=(CRSite (CStringList) "SbfI" "cctgcagg" 7 0 1 1823 0 0 "") 
COMMENT        #131=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 5476 0 0 "") 
COMMENT        #132=(CRSite (CStringList) "SexAI" "accwggt" 2 0 0 0 "") 
COMMENT        #133=(CRSite (CStringList) "SfcI" "ctryag" 2 0 9 1069 0 1576 0 1819 0 
COMMENT              2472 0 2680 0 4368 0 4559 0 5237 0 6357 0 0 "") 
COMMENT        #134=(CRSite (CStringList) "SfiI" "ggccnnnnnggcc" 9 0 0 0 "") 
COMMENT        #135=(CRSite (CStringList) "SfoI" "ggcgcc" 4 0 2 2313 0 2421 0 0 "") 
COMMENT        #136=(CRSite (CStringList) "SgrAI" "crccggyg" 3 0 1 2215 0 0 "") 
COMMENT        #137=(CRSite (CStringList) "SmaI" "cccggg" 4 0 5 32 0 43 0 3489 0 3885 0 
COMMENT              3896 0 0 "") 
COMMENT        #138=(CRSite (CStringList) "SmlI" "ctyrag" 2 0 7 903 0 2413 0 2662 0 
COMMENT              4209 0 4471 0 4748 0 5616 0 0 "") 
COMMENT        #139=(CRSite (CStringList) "SnaBI" "tacgta" 4 0 3 168 0 416 0 3760 0 0 
COMMENT              "") #140=(CRSite (CStringList) "SpeI" "actagt" 2 0 0 0 "") 
COMMENT        #141=(CRSite (CStringList) "SphI" "gcatgc" 6 0 0 0 "") 
COMMENT        #142=(CRSite (CStringList) "SrfI" "gcccgggc" 5 0 4 32 0 43 0 3885 0 3896 
COMMENT              0 0 "") 
COMMENT        #143=(CRSite (CStringList) "SspI" "aatatt" 4 0 2 5800 0 5930 0 0 "") 
COMMENT        #144=(CRSite (CStringList) "StuI" "aggcct" 4 0 2 1949 0 2042 0 0 "") 
COMMENT        #145=(CRSite (CStringList) "StyI" "ccwwgg" 2 0 7 436 0 1385 0 1632 0 
COMMENT              1737 0 1950 0 2010 0 3292 0 0 "") 
COMMENT        #146=(CRSite (CStringList) "SwaI" "atttaaat" 5 0 0 0 "") 
COMMENT        #147=(CRSite (CStringList) "Tth111I" "gacnnngtc" 5 0 1 2284 0 0 "") 
COMMENT        #148=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 1027 0 0 "") 
COMMENT        #149=(CRSite (CStringList) "XcmI" "ccannnnnnnnntgg" 9 0 1 2215 0 0 "") 
COMMENT        #150=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 0 0 "") 
COMMENT        #151=(CRSite (CStringList) "XmaI" "cccggg" 2 0 5 30 0 41 0 3487 0 3883 0 
COMMENT              3894 0 0 "") 
COMMENT        #152=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 2 2854 0 5595 0 0 "")) 
COMMENT       (CObList 
COMMENT        #153=(CFSignal (CObList) "5' ITR" 21 0 0 1 168 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "1..168") 
COMMENT        #154=(CFSignal (CObList) "3' ITR" 21 0 0 3759 3925 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "3760..3926") 
COMMENT        #155=(CFSignal (CObList) "CAG promoter" 29 0 0 193 1126 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "193..1127") 
COMMENT        #156=(CFSignal (CObList) "AmpR" 21 0 1 4925 5782 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "complement(4926..5783)") 
COMMENT        #157=(CFSignal (CObList) "loxP" 21 0 0 1231 1264 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "1232..1265") 
COMMENT        #158=(CFSignal (CObList) "loxP" 21 0 0 2808 2841 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "2809..2842") 
COMMENT        #159=(CFSignal (CObList) "loxP2272" 21 0 1 1135 1168 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "complement(1136..1169)") 
COMMENT        #160=(CFSignal (CObList) "loxP2272" 21 0 0 2704 2737 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "2705..2738") 
COMMENT        #161=(CFSignal (CObList) "NES-jRGECO1b" 21 0 1 1275 2687 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "complement(1276..2688)") 
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COMMENT                              "AseI: 2 sites") 2 4 "ATTAAT" "TAATTA") 
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COMMENT                              "AvaI: 8 sites") 1 5 "CYCGRG" "GRGCYC") 
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COMMENT                             "ACNNNNGTAYCNNNNNNNNNNNNN" 
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COMMENT                              "BamHI: 2 sites") 1 5 "GGATCC" "CCTAGG") 
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COMMENT                              "BanI: 5 sites") 1 5 "GGYRCC" "CCRYGG") 
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COMMENT                                       #343=(CLinePar 
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COMMENT                                       #344=(CLinePar 
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COMMENT                              "BanII: 7 sites") 5 1 "GRGCYC" "CYCGRG") 
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COMMENT                                       #350=(CLinePar 
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COMMENT                                             " N2: 1735 (on complementary strand)" 
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COMMENT                                             " N3: 3412 (on complementary strand)" 
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COMMENT                             "GAAGACNNNNNNN" "CTTCTGNNNNNNN") 
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COMMENT                              "BbvCI: 1 site") 2 5 "CCTCAGC" "GGAGTCG") 
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COMMENT                                             " N1: 1205 (on complementary strand)" 
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COMMENT                                       #359=(CLinePar 
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COMMENT                                             " N3: 2756 (on complementary strand)" 
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COMMENT                              "BcgI: 4 sites") 24 22 "CGANNNNNNTGCNNNNNNNNNNNNN" 
COMMENT                             "GCTNNNNNNACGNNNNNNNNNNNNN") 
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COMMENT                                       #364=(CLinePar 
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COMMENT                                             " N2: 2712 (on complementary strand)" 
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COMMENT                                       #365=(CLinePar 
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COMMENT                                             " N3: 2953 (on complementary strand)" 
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COMMENT                                       #366=(CLinePar 
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COMMENT                                             " N4: 4306 (on complementary strand)" 
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COMMENT                                             " N5: 5833 (on complementary strand)" 
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COMMENT                             "GTATCCNNNNNNN" "CATAGGNNNNNNN") 
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COMMENT                                       #371=(CLinePar 
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COMMENT                              "BclI: 2 sites") 1 5 "TGATCA" "ACTAGT") 
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COMMENT                              "BfuAI: 1 site") 10 14 "ACCTGCNNNNNNNNN" 
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COMMENT                                       #379=(CLinePar 
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COMMENT                                       #380=(CLinePar 
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COMMENT                                       #381=(CLinePar 
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COMMENT                                       #382=(CLinePar 
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COMMENT                                       #383=(CLinePar 
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COMMENT                              "BglI: 6 sites") 7 4 "GCCNNNNNGGC" "CGGNNNNNCCG") 
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COMMENT                              "BglII: 1 site") 1 5 "AGATCT" "TCTAGA") 
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COMMENT                                       #390=(CLinePar 
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COMMENT                              "BlpI: 2 sites") 2 5 "GCTNAGC" "CGANTCG") 
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COMMENT                                      (CObjectList 
COMMENT                                       #393=(CLinePar 
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COMMENT                                             " N1: 2513 (on complementary strand)" 
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COMMENT                             "CACGTC" "GTGCAG") 
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COMMENT                               (CObjectList 
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COMMENT                                      (CObjectList 
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COMMENT                                             " N1: 365 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #397=(CLinePar 
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COMMENT                                              191) 
COMMENT                                             " N2: 5036 (on complementary strand)" 
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COMMENT                             "ACTGGGNNNNNN" "TGACCCNNNNNN") 
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COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 59269984 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
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COMMENT                                      (CObjectList 
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COMMENT                                             " N1: 2616 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #401=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #36# 2) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N2: 5066 (on complementary strand)" 
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COMMENT                             "CTGGAGNNNNNNNNNNNNNNNNN" "GACCTCNNNNNNNNNNNNNNNNN") 
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COMMENT                              (CGroupPar (CParagraph 59270016 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
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COMMENT                                      (CParagraph 0 (10 #37# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #404=(CLinePar 
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COMMENT                              "Bpu10I: 1 site") 2 5 "CCTNAGC" "GGANTCG") 
COMMENT                       #405=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 59270048 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #406=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #38# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #407=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #38# 1) 1 2 2 0 
COMMENT                                              191) " N1: 924 " 1) 
COMMENT                                       #408=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #38# 2) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N2: 4194 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #409=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #38# 3) 1 2 2 0 
COMMENT                                              191) " N3: 4492 " 1) 
COMMENT                                       #410=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #38# 4) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N4: 4733 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #411=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #38# 5) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N5: 5601 (on complementary strand)" 
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COMMENT                             "CTTGAGNNNNNNNNNNNNNNNNN" "GAACTCNNNNNNNNNNNNNNNNN") 
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COMMENT                              (CGroupPar (CParagraph 59268672 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #413=(CGroupPar 
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COMMENT                                      (CObjectList 
COMMENT                                       #414=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #39# 1) 1 2 2 0 
COMMENT                                              191) " N1: 168 " 1) 
COMMENT                                       #415=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #39# 2) 1 2 2 0 
COMMENT                                              191) " N2: 416 " 1) 
COMMENT                                       #416=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #39# 3) 1 2 2 0 
COMMENT                                              191) " N3: 1401 " 1) 
COMMENT                                       #417=(CLinePar 
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COMMENT                                              191) " N4: 1842 " 1) 
COMMENT                                       #418=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #39# 5) 1 2 2 0 
COMMENT                                              191) " N5: 3760 " 1) 
COMMENT                                       #419=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #39# 6) 1 2 2 0 
COMMENT                                              191) " N6: 6138 " 1))))) 
COMMENT                              "BsaAI: 6 sites") 3 3 "YACGTR" "RTGCAY") 
COMMENT                       #420=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 59268704 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #421=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #40# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #422=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #40# 1) 1 2 2 0 
COMMENT                                              191) " N1: 2542 " 1) 
COMMENT                                       #423=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #40# 2) 1 2 2 0 
COMMENT                                              191) " N2: 3737 " 1))))) 
COMMENT                              "BsaBI: 2 sites") 5 5 "GATNNNNATC" "CTANNNNTAG") 
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COMMENT                              (CGroupPar (CParagraph 59268736 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #425=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #41# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #426=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #41# 1) 1 2 2 0 
COMMENT                                              191) " N1: 46 " 1) 
COMMENT                                       #427=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #41# 2) 1 2 2 0 
COMMENT                                              191) " N2: 198 " 1) 
COMMENT                                       #428=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #41# 3) 1 2 2 0 
COMMENT                                              191) " N3: 251 " 1) 
COMMENT                                       #429=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #41# 4) 1 2 2 0 
COMMENT                                              191) " N4: 334 " 1) 
COMMENT                                       #430=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #41# 5) 1 2 2 0 
COMMENT                                              191) " N5: 2312 " 1) 
COMMENT                                       #431=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #41# 6) 1 2 2 0 
COMMENT                                              191) " N6: 2420 " 1) 
COMMENT                                       #432=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #41# 7) 1 2 2 0 
COMMENT                                              191) " N7: 3334 " 1) 
COMMENT                                       #433=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #41# 8) 1 2 2 0 
COMMENT                                              191) " N8: 3880 " 1) 
COMMENT                                       #434=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #41# 9) 1 2 2 0 
COMMENT                                              191) " N9: 5533 " 1))))) 
COMMENT                              "BsaHI: 9 sites") 2 4 "GRCGYC" "CYGCRG") 
COMMENT                       #435=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 59268768 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #436=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #42# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #437=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #42# 1) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N1: 5057 (on complementary strand)" 
COMMENT                                             1))))) "BsaI: 1 site") 7 11 
COMMENT                             "GGTCTCNNNNNN" "CCAGAGNNNNNN") 
COMMENT                       #438=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 59268800 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #439=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #43# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #440=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #43# 1) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N1: 1820 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #441=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #43# 2) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N2: 2270 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #442=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #43# 3) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N3: 3948 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #443=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #43# 4) 1 2 2 0 
COMMENT                                              191) " N4: 6102 " 1))))) 
COMMENT                              "BsaXI: 4 sites") 21 18 "ACNNNNNCTCCNNNNNNNNNNN" 
COMMENT                             "TGNNNNNGAGGNNNNNNNNNNN") 
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COMMENT                                             " N1: 826 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #447=(CLinePar 
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COMMENT                                             " N2: 1354 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #448=(CLinePar 
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COMMENT                                       #449=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #44# 4) 1 2 2 0 
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COMMENT                                             " N4: 2146 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #450=(CLinePar 
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COMMENT                                             " N5: 2619 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #451=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #44# 6) 1 2 2 0 
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COMMENT                                             " N6: 3009 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #452=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #44# 7) 1 2 2 0 
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COMMENT                              "BseRI: 7 sites") 16 14 "GAGGAGNNNNNNNNNNN" 
COMMENT                             "CTCCTCNNNNNNNNNNN") 
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COMMENT                                       #456=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #45# 2) 1 2 2 0 
COMMENT                                              191) " N2: 1731 " 1) 
COMMENT                                       #457=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #45# 3) 1 2 2 0 
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COMMENT                                             " N3: 1931 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #458=(CLinePar 
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COMMENT                                             " N4: 4407 (on complementary strand)" 
COMMENT                                             1))))) "BseYI: 4 sites") 1 5 
COMMENT                             "CCCAGC" "GGGTCG") 
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COMMENT                              (CGroupPar (CParagraph 59268576 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
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COMMENT                                      (CObjectList 
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COMMENT                                             (CParagraph 0 (1 #46# 1) 1 2 2 0 
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COMMENT                                       #462=(CLinePar 
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COMMENT                                             " N2: 2308 (on complementary strand)" 
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COMMENT                             "GTGCAGNNNNNNNNNNNNNNNNN" "CACGTCNNNNNNNNNNNNNNNNN") 
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COMMENT                                             (CParagraph 0 (1 #47# 1) 1 2 2 0 
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COMMENT                                       #466=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #47# 2) 1 2 2 0 
COMMENT                                              191) " N2: 3496 " 1) 
COMMENT                                       #467=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #47# 3) 1 2 2 0 
COMMENT                                              191) " N3: 4019 " 1) 
COMMENT                                       #468=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #47# 4) 1 2 2 0 
COMMENT                                              191) " N4: 4443 " 1) 
COMMENT                                       #469=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #47# 5) 1 2 2 0 
COMMENT                                              191) " N5: 5366 " 1) 
COMMENT                                       #470=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #47# 6) 1 2 2 0 
COMMENT                                              191) " N6: 5515 " 1) 
COMMENT                                       #471=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #47# 7) 1 2 2 0 
COMMENT                                              191) " N7: 6411 " 1))))) 
COMMENT                              "BsiEI: 7 sites") 4 2 "CGRYCG" "GCYRGC") 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103646416 (8 0) 1 1 1 0 178) 
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COMMENT                                      (CParagraph 0 (10 #48# 0) 1 2 2 0 180) 
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COMMENT                                             (CParagraph 0 (1 #48# 1) 1 2 2 0 
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COMMENT                                       #475=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #48# 2) 1 2 2 0 
COMMENT                                              191) " N2: 2463 " 1) 
COMMENT                                       #476=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #48# 3) 1 2 2 0 
COMMENT                                              191) " N3: 3097 " 1) 
COMMENT                                       #477=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #48# 4) 1 2 2 0 
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COMMENT                                       #478=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #48# 5) 1 2 2 0 
COMMENT                                              191) " N5: 5582 " 1) 
COMMENT                                       #479=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #48# 6) 1 2 2 0 
COMMENT                                              191) " N6: 5667 " 1))))) 
COMMENT                              "BsiHKAI: 6 sites") 5 1 "GWGCWC" "CWCGWG") 
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COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103646576 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsiWI: 0 sites") 1 5 "CGTACG" 
COMMENT                             "GCATGC") 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103646608 (8 0) 1 1 1 0 178) 
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COMMENT                                #482=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #50# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #483=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #50# 1) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N1: 2273 (on complementary strand)" 
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COMMENT                             "CGTCTCNNNNNN" "GCAGAGNNNNNN") 
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COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103646640 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
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COMMENT                                      (CParagraph 0 (10 #51# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #486=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #51# 1) 1 2 2 0 
COMMENT                                              191) " N1: 3557 " 1) 
COMMENT                                       #487=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #51# 2) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N2: 3650 (on complementary strand)" 
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COMMENT                             "GAATGCNN" "CTTACGNN") 
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COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 58144744 (8 0) 1 1 1 0 178) 
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COMMENT                                      (CParagraph 0 (10 #52# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
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COMMENT                                             (CParagraph 0 (1 #52# 1) 1 2 2 0 
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COMMENT                                       #491=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #52# 2) 1 2 2 0 
COMMENT                                              191) " N2: 751 " 1) 
COMMENT                                       #492=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #52# 3) 1 2 2 0 
COMMENT                                              191) " N3: 913 " 1) 
COMMENT                                       #493=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #52# 4) 1 2 2 0 
COMMENT                                              191) " N4: 924 " 1) 
COMMENT                                       #494=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #52# 5) 1 2 2 0 
COMMENT                                              191) " N5: 2103 " 1) 
COMMENT                                       #495=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #52# 6) 1 2 2 0 
COMMENT                                              191) " N6: 2114 " 1) 
COMMENT                                       #496=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #52# 7) 1 2 2 0 
COMMENT                                              191) " N7: 2463 " 1) 
COMMENT                                       #497=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #52# 8) 1 2 2 0 
COMMENT                                              191) " N8: 3097 " 1) 
COMMENT                                       #498=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #52# 9) 1 2 2 0 
COMMENT                                              191) " N9: 3237 " 1) 
COMMENT                                       #499=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #52# 10) 1 2 2 0 
COMMENT                                              191) " N10: 3250 " 1) 
COMMENT                                       #500=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #52# 11) 1 2 2 0 
COMMENT                                              191) " N11: 4421 " 1) 
COMMENT                                       #501=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #52# 12) 1 2 2 0 
COMMENT                                              191) " N12: 5582 " 1) 
COMMENT                                       #502=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #52# 13) 1 2 2 0 
COMMENT                                              191) " N13: 5667 " 1) 
COMMENT                                       #503=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #52# 14) 1 2 2 0 
COMMENT                                              191) " N14: 6213 " 1))))) 
COMMENT                              "Bsp1286I: 14 sites") 5 1 "GDGCHC" "CHCGDG") 
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COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 58144704 (8 0) 1 1 1 0 178) 
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COMMENT                                #505=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #53# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
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COMMENT                                             (CParagraph 0 (1 #53# 1) 1 2 2 0 
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COMMENT                                       #507=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #53# 2) 1 2 2 0 
COMMENT                                              191) " N2: 2879 " 1) 
COMMENT                                       #508=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #53# 3) 1 2 2 0 
COMMENT                                              191) " N3: 3474 " 1) 
COMMENT                                       #509=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #53# 4) 1 2 2 0 
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COMMENT                              "BspDI: 4 sites") 2 4 "ATCGAT" "TAGCTA") 
COMMENT                       #510=(CRSFolderPar 
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COMMENT                              (CGroupPar 
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COMMENT                                      (CObjectList 
COMMENT                                       #512=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #54# 1) 1 2 2 0 
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COMMENT                              "BspEI: 1 site") 1 5 "TCCGGA" "AGGCCT") 
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COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103646832 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #514=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #55# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #515=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #55# 1) 1 2 2 0 
COMMENT                                              191) " N1: 4823 " 1) 
COMMENT                                       #516=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #55# 2) 1 2 2 0 
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COMMENT                              "BspHI: 2 sites") 1 5 "TCATGA" "AGTACT") 
COMMENT                       #517=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103646864 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
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COMMENT                                      (CParagraph 0 (10 #56# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #519=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #56# 1) 1 2 2 0 
COMMENT                                              191) " N1: 2500 " 1))))) 
COMMENT                              "BspMI: 1 site") 10 14 "ACCTGCNNNNNNNNN" 
COMMENT                             "TGGACGNNNNNNNNN") 
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COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103643664 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #521=(CGroupPar 
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COMMENT                                      (CObjectList 
COMMENT                                       #522=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #57# 1) 1 2 2 0 
COMMENT                                              191) " N1: 640 " 1) 
COMMENT                                       #523=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #57# 2) 1 2 2 0 
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COMMENT                                             " N2: 754 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #524=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #57# 3) 1 2 2 0 
COMMENT                                              191) " N3: 817 " 1) 
COMMENT                                       #525=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #57# 4) 1 2 2 0 
COMMENT                                              191) " N4: 940 " 1) 
COMMENT                                       #526=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #57# 5) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N5: 2442 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #527=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #57# 6) 1 2 2 0 
COMMENT                                              191) " N6: 4036 " 1) 
COMMENT                                       #528=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #57# 7) 1 2 2 0 
COMMENT                                              191) " N7: 5837 " 1) 
COMMENT                                       #529=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #57# 8) 1 2 2 0 
COMMENT                                              191) " N8: 6282 " 1))))) 
COMMENT                              "BsrBI: 8 sites") 3 3 "GAGCGG" "CTCGCC") 
COMMENT                       #530=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103643696 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #531=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #58# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #532=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #58# 1) 1 2 2 0 
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COMMENT                                             " N1: 1430 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #533=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #58# 2) 1 2 2 0 
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COMMENT                                             " N2: 3131 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #534=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #58# 3) 1 2 2 0 
COMMENT                                              191) " N3: 5057 " 1) 
COMMENT                                       #535=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #58# 4) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N4: 5231 (on complementary strand)" 
COMMENT                                             1))))) "BsrDI: 4 sites") 8 6 
COMMENT                             "GCAATGNNN" "CGTTACNNN") 
COMMENT                       #536=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103647504 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #537=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #59# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #538=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #59# 1) 1 2 2 0 
COMMENT                                              191) " N1: 1018 " 1) 
COMMENT                                       #539=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #59# 2) 1 2 2 0 
COMMENT                                              191) " N2: 2215 " 1) 
COMMENT                                       #540=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #59# 3) 1 2 2 0 
COMMENT                                              191) " N3: 3396 " 1) 
COMMENT                                       #541=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #59# 4) 1 2 2 0 
COMMENT                                              191) " N4: 5076 " 1) 
COMMENT                                       #542=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #59# 5) 1 2 2 0 
COMMENT                                              191) " N5: 6239 " 1))))) 
COMMENT                              "BsrFI: 5 sites") 1 5 "RCCGGY" "YGGCCR") 
COMMENT                       #543=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103647536 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #544=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #60# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #545=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #60# 1) 1 2 2 0 
COMMENT                                              191) " N1: 1295 " 1) 
COMMENT                                       #546=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #60# 2) 1 2 2 0 
COMMENT                                              191) " N2: 2196 " 1))))) 
COMMENT                              "BsrGI: 2 sites") 1 5 "TGTACA" "ACATGT") 
COMMENT                       #547=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103647056 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #548=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #61# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #549=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #61# 1) 1 2 2 0 
COMMENT                                              191) " N1: 4 " 1) 
COMMENT                                       #550=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #61# 2) 1 2 2 0 
COMMENT                                              191) " N2: 89 " 1) 
COMMENT                                       #551=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #61# 3) 1 2 2 0 
COMMENT                                              191) " N3: 563 " 1) 
COMMENT                                       #552=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #61# 4) 1 2 2 0 
COMMENT                                              191) " N4: 565 " 1) 
COMMENT                                       #553=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #61# 5) 1 2 2 0 
COMMENT                                              191) " N5: 643 " 1) 
COMMENT                                       #554=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #61# 6) 1 2 2 0 
COMMENT                                              191) " N6: 706 " 1) 
COMMENT                                       #555=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #61# 7) 1 2 2 0 
COMMENT                                              191) " N7: 729 " 1) 
COMMENT                                       #556=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #61# 8) 1 2 2 0 
COMMENT                                              191) " N8: 3835 " 1) 
COMMENT                                       #557=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #61# 9) 1 2 2 0 
COMMENT                                              191) " N9: 3920 " 1))))) 
COMMENT                              "BssHII: 9 sites") 1 5 "GCGCGC" "CGCGCG") 
COMMENT                       #558=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103647088 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #559=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #62# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #560=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #62# 1) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N1: 2083 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #561=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #62# 2) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N2: 4276 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #562=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #62# 3) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N3: 5660 (on complementary strand)" 
COMMENT                                             1))))) "BssSI: 3 sites") 1 5 
COMMENT                             "CACGAG" "GTGCTC") 
COMMENT                       #563=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103647120 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstAPI: 0 sites") 7 4 
COMMENT                             "GCANNNNNTGC" "CGTNNNNNACG") 
COMMENT                       #564=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103647152 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #565=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #64# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #566=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #64# 1) 1 2 2 0 
COMMENT                                              191) " N1: 2858 " 1))))) 
COMMENT                              "BstBI: 1 site") 2 4 "TTCGAA" "AAGCTT") 
COMMENT                       #567=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103647184 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #568=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #65# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #569=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #65# 1) 1 2 2 0 
COMMENT                                              191) " N1: 2014 " 1))))) 
COMMENT                              "BstEII: 1 site") 1 6 "GGTNACC" "CCANTGG") 
COMMENT                       #570=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103647568 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstXI: 0 sites") 8 4 
COMMENT                             "CCANNNNNNTGG" "GGTNNNNNNACC") 
COMMENT                       #571=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103647728 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #572=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #67# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #573=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #67# 1) 1 2 2 0 
COMMENT                                              191) " N1: 1128 " 1) 
COMMENT                                       #574=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #67# 2) 1 2 2 0 
COMMENT                                              191) " N2: 2537 " 1) 
COMMENT                                       #575=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #67# 3) 1 2 2 0 
COMMENT                                              191) " N3: 2561 " 1) 
COMMENT                                       #576=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #67# 4) 1 2 2 0 
COMMENT                                              191) " N4: 2695 " 1) 
COMMENT                                       #577=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #67# 5) 1 2 2 0 
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COMMENT                              "HpaI: 2 sites") 3 3 "GTTAAC" "CAATTG") 
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COMMENT                             (CFolderPar 
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COMMENT                               (CParagraph 103646544 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "I-SceI: 0 sites") 9 5 
COMMENT                             "TWGGGATAACAGGGTAAT" "AWCCCTATTGTCCCATTA") 
COMMENT                       #714=(CRSFolderPar 
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COMMENT                                       #717=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #94# 2) 1 2 2 0 
COMMENT                                              191) " N2: 2419 " 1))))) 
COMMENT                              "KasI: 2 sites") 1 5 "GGCGCC" "CCGCGG") 
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COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103642384 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "KpnI: 0 sites") 5 1 "GGTACC" 
COMMENT                             "CCATGG") 
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COMMENT                              "MfeI: 1 site") 1 5 "CAATTG" "GTTAAC") 
COMMENT                       #722=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103642448 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
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COMMENT                                      (CParagraph 0 (10 #97# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
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COMMENT                                             (CParagraph 0 (1 #97# 1) 1 2 2 0 
COMMENT                                              191) " N1: 1720 " 1))))) 
COMMENT                              "MluI: 1 site") 1 5 "ACGCGT" "TGCGCA") 
COMMENT                       #725=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103642480 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
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COMMENT                                      (CParagraph 0 (10 #98# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
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COMMENT                                       #728=(CLinePar 
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COMMENT                                              191) " N2: 2144 " 1) 
COMMENT                                       #729=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #98# 3) 1 2 2 0 
COMMENT                                              191) " N3: 3821 " 1))))) 
COMMENT                              "MscI: 3 sites") 3 3 "TGGCCA" "ACCGGT") 
COMMENT                       #730=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103643280 (8 0) 1 1 1 0 178) 
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COMMENT                                       #733=(CLinePar 
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COMMENT                                              191) " N2: 2258 " 1) 
COMMENT                                       #734=(CLinePar 
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COMMENT                                              191) " N3: 2608 " 1) 
COMMENT                                       #735=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #99# 4) 1 2 2 0 
COMMENT                                              191) " N4: 5248 " 1) 
COMMENT                                       #736=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #99# 5) 1 2 2 0 
COMMENT                                              191) " N5: 5407 " 1) 
COMMENT                                       #737=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #99# 6) 1 2 2 0 
COMMENT                                              191) " N6: 5766 " 1))))) 
COMMENT                              "MslI: 6 sites") 5 5 "CAYNNNNRTG" "GTRNNNNYAC") 
COMMENT                       #738=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103643312 (8 0) 1 1 1 0 178) 
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COMMENT                                       #741=(CLinePar 
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COMMENT                                              191) " N2: 957 " 1) 
COMMENT                                       #742=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #100# 3) 1 2 2 0 
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COMMENT                                       #743=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #100# 4) 1 2 2 0 
COMMENT                                              191) " N4: 3221 " 1) 
COMMENT                                       #744=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #100# 5) 1 2 2 0 
COMMENT                                              191) " N5: 3370 " 1) 
COMMENT                                       #745=(CLinePar 
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COMMENT                                              191) " N6: 3386 " 1) 
COMMENT                                       #746=(CLinePar 
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COMMENT                                              191) " N7: 3927 " 1) 
COMMENT                                       #747=(CLinePar 
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COMMENT                                              191) " N8: 4445 " 1) 
COMMENT                                       #748=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #100# 9) 1 2 2 0 
COMMENT                                              191) " N9: 4690 " 1) 
COMMENT                                       #749=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #100# 10) 1 2 2 0 
COMMENT                                              191) " N10: 5631 " 1))))) 
COMMENT                              "MspA1I: 10 sites") 3 3 "CMGCKG" "GKCGMC") 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103644560 (8 0) 1 1 1 0 178) 
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COMMENT                                      (CParagraph 0 (10 #101# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
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COMMENT                                       #753=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #101# 2) 1 2 2 0 
COMMENT                                              191) " N2: 6241 " 1))))) 
COMMENT                              "NaeI: 2 sites") 3 3 "GCCGGC" "CGGCCG") 
COMMENT                       #754=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103644592 (8 0) 1 1 1 0 178) 
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COMMENT                                       #757=(CLinePar 
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COMMENT                                              191) " N2: 2420 " 1))))) 
COMMENT                              "NarI: 2 sites") 2 4 "GGCGCC" "CCGCGG") 
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COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103645264 (8 0) 1 1 1 0 178) 
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COMMENT                                      (CParagraph 0 (10 #103# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
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COMMENT                                              191) " N1: 436 " 1) 
COMMENT                                       #761=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #103# 2) 1 2 2 0 
COMMENT                                              191) " N2: 1737 " 1))))) 
COMMENT                              "NcoI: 2 sites") 1 5 "CCATGG" "GGTACC") 
COMMENT                       #762=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103645296 (8 0) 1 1 1 0 178) 
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COMMENT                                      (CParagraph 0 (10 #104# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
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COMMENT                              "NdeI: 1 site") 2 4 "CATATG" "GTATAC") 
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COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103645712 (8 0) 1 1 1 0 178) 
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COMMENT                                      (CParagraph 0 (10 #105# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
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COMMENT                                       #768=(CLinePar 
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COMMENT                              "NgoMIV: 2 sites") 1 5 "GCCGGC" "CGGCCG") 
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COMMENT                              "NheI: 1 site") 1 5 "GCTAGC" "CGATCG") 
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COMMENT                             (CFolderPar 
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COMMENT                               (CParagraph 103645776 (8 0) 1 1 1 0 178) 
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COMMENT                              "NotI: 1 site") 2 6 "GCGGCCGC" "CGCCGGCG") 
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COMMENT                             (CFolderPar 
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COMMENT                               (CParagraph 103645808 (8 0) 1 1 1 0 178) 
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COMMENT                              "NruI: 1 site") 3 3 "TCGCGA" "AGCGCT") 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103645840 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "NsiI: 0 sites") 5 1 "ATGCAT" 
COMMENT                             "TACGTA") 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103645872 (8 0) 1 1 1 0 178) 
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COMMENT                              "NspI: 1 site") 5 1 "RCATGY" "YGTACR") 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103645904 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "PacI: 0 sites") 5 3 "TTAATTAA" 
COMMENT                             "AATTAATT") 
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COMMENT                             (CFolderPar 
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COMMENT                               (CParagraph 103645936 (8 0) 1 1 1 0 178) 
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COMMENT                              "PciI: 1 site") 1 5 "ACATGT" "TGTACA") 
COMMENT                       #786=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103645968 (8 0) 1 1 1 0 178) 
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COMMENT                              "PflFI: 1 site") 4 5 "GACNNNGTC" "CTGNNNCAG") 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103646000 (8 0) 1 1 1 0 178) 
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COMMENT                                       #792=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #114# 2) 1 2 2 0 
COMMENT                                              191) " N2: 3125 " 1))))) 
COMMENT                              "PflMI: 2 sites") 7 4 "CCANNNNNTGG" "GGTNNNNNACC") 
COMMENT                       #793=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103646032 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "PI-SceI: 0 sites") 15 11 
COMMENT                             "ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGGCA" 
COMMENT                             "TAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGT") 
COMMENT                       #794=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103646064 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "PmeI: 0 sites") 4 4 "GTTTAAAC" 
COMMENT                             "CAAATTTG") 
COMMENT                       #795=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103646096 (8 0) 1 1 1 0 178) 
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COMMENT                                       #798=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #117# 2) 1 2 2 0 
COMMENT                                              191) " N2: 1842 " 1))))) 
COMMENT                              "PmlI: 2 sites") 3 3 "CACGTG" "GTGCAC") 
COMMENT                       #799=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103646128 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "PpuMI: 0 sites") 2 5 "RGGWCCY" 
COMMENT                             "YCCWGGR") 
COMMENT                       #800=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103645200 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "PshAI: 0 sites") 5 5 "GACNNNNGTC" 
COMMENT                             "CTGNNNNCAG") 
COMMENT                       #801=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103644176 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
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COMMENT                                      (CObjectList 
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COMMENT                                       #804=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #120# 2) 1 2 2 0 
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COMMENT                              "PsiI: 2 sites") 3 3 "TTATAA" "AATATT") 
COMMENT                       #805=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103644208 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
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COMMENT                                      (CObjectList 
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COMMENT                              "PspOMI: 1 site") 1 5 "GGGCCC" "CCCGGG") 
COMMENT                       #808=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103644240 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
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COMMENT                                      (CObjectList 
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COMMENT                                       #811=(CLinePar 
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COMMENT                                       #812=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #122# 3) 1 2 2 0 
COMMENT                                              191) " N3: 2684 " 1))))) 
COMMENT                              "PstI: 3 sites") 5 1 "CTGCAG" "GACGTC") 
COMMENT                       #813=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103644272 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
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COMMENT                                      (CObjectList 
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COMMENT                                       #816=(CLinePar 
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COMMENT                                       #817=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #123# 3) 1 2 2 0 
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COMMENT                              "PvuI: 3 sites") 4 2 "CGATCG" "GCTAGC") 
COMMENT                       #818=(CRSFolderPar 
COMMENT                             (CFolderPar 
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COMMENT                               (CParagraph 103644624 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
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COMMENT                                      (CObjectList 
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COMMENT                                       #821=(CLinePar 
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COMMENT                                       #822=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #124# 3) 1 2 2 0 
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COMMENT                              "PvuII: 3 sites") 3 3 "CAGCTG" "GTCGAC") 
COMMENT                       #823=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103644656 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "RsrII: 0 sites") 2 5 "CGGWCCG" 
COMMENT                             "GCCWGGC") 
COMMENT                       #824=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103645392 (8 0) 1 1 1 0 178) 
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COMMENT                                              191) " N1: 2463 " 1))))) 
COMMENT                              "SacI: 1 site") 5 1 "GAGCTC" "CTCGAG") 
COMMENT                       #827=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 103645424 (8 0) 1 1 1 0 178) 
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COMMENT                                       #830=(CLinePar 
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COMMENT                              "SacII: 2 sites") 4 2 "CCGCGG" "GGCGCC") 
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COMMENT                                       #834=(CLinePar 
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COMMENT                              "SalI: 2 sites") 1 5 "GTCGAC" "CAGCTG") 
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COMMENT                                       #838=(CLinePar 
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COMMENT                              "SapI: 2 sites") 8 11 "GCTCTTCNNNNN" 
COMMENT                             "CGAGAAGNNNNN") 
COMMENT                       #839=(CRSFolderPar 
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COMMENT                               (CParagraph 103642288 (8 0) 1 1 1 0 178) 
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COMMENT                              "SbfI: 1 site") 6 2 "CCTGCAGG" "GGACGTCC") 
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COMMENT                              "SfcI: 9 sites") 1 5 "CTRYAG" "GAYRTC") 
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COMMENT                              "XcmI: 1 site") 8 7 "CCANNNNNNNNNTGG" 
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FEATURES             Location/Qualifiers
     misc_feature    1..168
                     /vntifkey="21"
                     /label=5'\ITR
                     /note="3' ITR"
     misc_feature    3759..3925
                     /vntifkey="21"
                     /label=3'\ITR
                     /note="5' ITR"
     promoter        193..1126
                     /vntifkey="29"
                     /label=CAG\promoter
                     /note="CAG promoter"
     misc_feature    complement(4925..5782)
                     /vntifkey="21"
                     /label=AmpR
                     /note="AmpR"
     misc_feature    1231..1264
                     /vntifkey="21"
                     /label=loxP
                     /note="loxP"
     misc_feature    2808..2841
                     /vntifkey="21"
                     /label=loxP
                     /note="loxP"
     misc_feature    complement(1135..1168)
                     /vntifkey="21"
                     /label=loxP2272
                     /note="loxP2272"
     misc_feature    2704..2737
                     /vntifkey="21"
                     /label=loxP2272
                     /note="loxP2272"
     misc_feature    complement(1275..2687)
                     /vntifkey="21"
                     /label=NES-jRGECO1b
                     /note="NES-jRGECO1b"
     polyA_signal    3499..3731
                     /vntifkey="25"
                     /label=SV40\polyadenylation\signal
                     /note="SV40 polyadenylation signal"
     misc_feature    2883..3471
                     /vntifkey="21"
                     /label=WPRE
                     /note="WPRE"
     misc_feature    731..731
                     /vntifkey="21"
                     /label=Deletion\-1bp\(A)
BASE COUNT     1394 a      1754 c      1659 g      1632 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagagta ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 
      241 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 
      301 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 
      361 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt 
      421 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc 
      481 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat 
      541 gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg 
      601 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc 
      661 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg 
      721 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc 
      781 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 
      841 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag 
      901 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggc tgtccgcggg 
      961 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg 
     1021 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg 
     1081 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattggat ccccataact 
     1141 tcgtataaag tatcctatac gaagttatat caaaatagga agaccaatgc ttcaccatcg 
     1201 acccgaattg ccaagcatca ccatcgaccc ataacttcgt ataatgtatg ctatacgaag 
     1261 ttatactaga gctttcactt cgctgtcatc atttgtacaa actcttcgta gtttacctga 
     1321 ccatccccat cgatgtctgc taccctgatc atttcatcaa cctcctcatc tgttaacttc 
     1381 tctccaaggt ctgtcatcac gtggcgaagc tctgctgcgc cgatgtagcc attgccgtcc 
     1441 ttatcaaaca cgcggaacgc ttcgcgaatt tcctcttcac tgtctgtgtc attcattttt 
     1501 cttgccatca tcgtcaggaa ctcagggaag tcgaatgtgc cgtcaccgtc ggcatctact 
     1561 tcattgatca tgtcctgcag ctctgcttct gtggggttct gccccagcga tcgcatcacc 
     1621 gtcccaagct ccttggttgt tatcgtccca tccccgtcct tgtcaaagca ggagaaagcc 
     1681 tctttaaatt ctgcgatctg ctcttcagtc agttggtcac gcgtagcctc ccagcccatg 
     1741 gtcttcttct gcattacggg gccgtccgga gggaagttgg tgccgcgcag cttcaccttg 
     1801 tagatgaata cgccgtcctg cagggaggag tcctggttca cgtgaataat gccgccgtcc 
     1861 tcgaagttca tcacgcgctc ccacctgaag ccctcgggga aggacagctt gaagtagtcg 
     1921 gggatgtcgg ctgggtgctt aatgtaggcc ttggagccgt acatgaactg aggggacagg 
     1981 atgtcccagg cgaagggcag ggggccaccc ttggtcacct tcagcttagc ggtctgaaag 
     2041 gcctcgtagg ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcacggag 
     2101 ccctccatgt gcaccttgaa gcgcatgaac tccttgatga tggccatgtt atcctcctcg 
     2161 cccttgctca ccagactccc gcctgtacct cccttgtaca gctcgtccat gccgccggtg 
     2221 gagtggcggc cctcggcaca ttcgcactgt tccacgatgg tgtagtcctc gttgtgggag 
     2281 acgatgtcca acttgatgtc caccttgtag gcgccgggca gctgcacggg cttcttggcc 
     2341 ttgtaggtag tcttgacctc ggcggcgtag tggccgccgt ccttcagcct cagccccttc 
     2401 ttgatctcgc tcttaagggc gccgtcctcg gggtacatcc gctcggaaac cacgggtgag 
     2461 ctcagccgac ctatagctct gactgcgtga cctgccttat tccacttacg acgtgatgag 
     2521 tcgaccattg ttgcgagatc cttatcgtca tcgtcgtaca gatcccgacc catttgctgt 
     2581 ccaccagtca tgctagccat accatgatga tgatgatgat gagaacctcc tccagtcttg 
     2641 ttaatatcaa gtccagccaa cttaagagca agctcgttct gcagcatggt ggcggatcct 
     2701 agtataactt cgtataggat actttatacg aagttatcat tgggattctt cctattttga 
     2761 tccaagcatc accatcgacc ctctagtcca gatctcacca tcgacccata acttcgtata 
     2821 gcatacatta tacgaagtta tgtccctcga agaggttcga attcgatatc aagcttatcg 
     2881 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 
     2941 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 
     3001 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 
     3061 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 
     3121 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 
     3181 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 
     3241 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc 
     3301 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 
     3361 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 
     3421 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc 
     3481 gtcgacccgg gcggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa 
     3541 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt 
     3601 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta 
     3661 tgtttcaggt tcagggggag atgtgggagg ttttttaaag caagtaaaac ctctacaaat 
     3721 gtggtaaaat cgataaggat cttcctagag catggctacg tagataagta gcatggcggg 
     3781 ttaatcatta actacaagga acccctagtg atggagttgg ccactccctc tctgcgcgct 
     3841 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg 
     3901 gcctcagtga gcgagcgagc gcgcagctgc attaatgaat cggccaacgc gcggggagag 
     3961 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 
     4021 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 
     4081 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 
     4141 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 
     4201 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 
     4261 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 
     4321 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 
     4381 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 
     4441 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 
     4501 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 
     4561 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 
     4621 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 
     4681 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 
     4741 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 
     4801 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 
     4861 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 
     4921 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 
     4981 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 
     5041 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 
     5101 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 
     5161 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 
     5221 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 
     5281 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 
     5341 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 
     5401 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 
     5461 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 
     5521 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 
     5581 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 
     5641 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 
     5701 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 
     5761 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 
     5821 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 
     5881 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa 
     5941 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa 
     6001 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa 
     6061 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca 
     6121 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg 
     6181 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc 
     6241 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc 
     6301 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca 
     6361 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 
     6421 ctcttcgcta ttacgccag 
//
