LOCUS       pAAV.Syn.Flex.NES-jRGECO1b.WPRE.SV40(p3845)        5999 bp    DNA     circular     18-JUN-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|737029171|
COMMENT     VNTDBDATE|737034418|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV.Syn.Flex.NES-jRGECO1b.WPRE.SV40(p3845)|
COMMENT     VNTAUTHORNAME|Erich Kurtz|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAAV.Syn.Flex.NES-jRGECO1b.WPRE.SV40(p3845)" 0 5999 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "AatI" "AatII" "Acc65I" "AccI" "AclI" "Acu I" "AfeI" 
COMMENT         "AflII" "AflIII" "AgeI" "AhdI" "AleI" "AlwNI" "ApaI" "ApaLI" "AscI" 
COMMENT         "AseI" "AsiSI" "AvaI" "AvrII" "BaeI" "BamHI" "BanI" "BanII" "BbsI" 
COMMENT         "BbvCI" "BcgI" "BciVI" "BclI" "BfrBI" "BfuAI" "BglI" "BglII" "BlpI" 
COMMENT         "BmgBI" "BmrI" "BpmI" "Bpu10I" "BpuEI" "BsaAI" "BsaBI" "BsaHI" "BsaI" 
COMMENT         "BsaXI" "BseRI" "BseYI" "BsgI" "BsiEI" "BsiHKAI" "BsiWI" "BsmBI" "BsmI" 
COMMENT         "Bsp1286I" "BspDI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrFI" 
COMMENT         "BsrGI" "BssHII" "BssSI" "BstAPI" "BstBI" "BstEII" "BstXI" "BstYI" 
COMMENT         "BstZ17I" "Bsu36I" "BtgI" "BtgZ I" "BtsCI" "BtsI" "ClaI (m)" 
COMMENT         "ClaI (nm)" "DraI" "DraIII" "DrdI" "EaeI" "EagI" "EarI" "EciI" "EcoNI" 
COMMENT         "EcoO109I" "EcoRI" "EcoRV" "FseI" "FspI" "HaeII" "HincII" "HindIII" 
COMMENT         "HpaI" "I-SceI" "KasI" "KpnI" "MfeI" "MluI" "MscI" "MslI" "MspA1I" 
COMMENT         "NaeI" "NarI" "NcoI" "NdeI" "NgoMIV" "NheI" "NotI" "NruI" "NsiI" "NspI" 
COMMENT         "PacI" "PciI" "PflFI" "PflMI" "PI-SceI" "PmeI" "PmlI" "PpuMI" "PshAI" 
COMMENT         "PsiI" "PspOMI" "PstI" "PvuI" "PvuII" "RsrII" "SacI" "SacII" "SalI" 
COMMENT         "SapI" "SbfI" "ScaI" "SexAI" "SfcI" "SfiI" "SfoI" "SgrAI" "SmaI" "SmlI" 
COMMENT         "SnaBI" "SpeI" "SphI" "SrfI" "SspI" "StuI" "StyI" "SwaI" "Tth111I" 
COMMENT         "XbaI" "XcmI" "XhoI" "XmaI" "XmnI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 5999 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "AatI" "aggcct" 4 0 2 1502 0 1595 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AatII" "gacgtc" 6 0 2 459 0 2890 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "AccI" "gtmkac" 3 0 2 2075 0 3036 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "AclI" "aacgtt" 3 0 2 4329 0 4702 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "Acu I" "ctgaag" 23 21 8 364 1 463 0 1242 1 
COMMENT            1460 0 1558 1 1921 1 4265 0 5277 1 0 "") 
COMMENT        #6=(CRSite (CStringList) "AfeI" "agcgct" 4 0 1 604 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "AflII" "cttaag" 2 0 2 1966 0 2215 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "AflIII" "acrygt" 2 0 2 1273 0 5819 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "AgeI" "accggt" 2 0 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "AhdI" "gacnnnnngtc" 7 0 4 59 0 1534 0 3428 0 
COMMENT             4931 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "AleI" "cacnnnngtg" 6 0 1 1811 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AlwNI" "cagnnnctg" 7 0 1 5410 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 202 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 4 1663 0 2646 0 4259 0 
COMMENT             5505 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "AseI" "attaat" 3 0 2 4756 0 5991 0 0 "") 
COMMENT        #17=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 1 1165 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 7 29 0 40 0 1447 0 1981 0 
COMMENT             3046 0 3442 0 3453 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "BaeI" "acnnnngtayc" 24 19 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 681 0 2248 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 7 252 0 588 0 1333 0 1864 
COMMENT             0 1972 0 3747 0 4978 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "BanII" "grgcyc" 6 0 5 202 0 1656 0 2016 0 
COMMENT             2790 0 3717 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "BbsI" "gaagac" 9 13 3 740 0 1288 1 2965 1 0 
COMMENT             "") 
COMMENT        #25=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 2 435 0 1943 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "BcgI" "cgannnnnntgc" 25 23 5 380 0 758 1 2143 
COMMENT             0 2309 1 4393 1 0 "") 
COMMENT        #27=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 5 715 0 2265 1 2506 1 
COMMENT             4094 0 5621 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "BclI" "tgatca" 2 0 2 900 0 1119 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BfuAI" "acctgc" 11 15 1 2053 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 4 31 0 3458 0 3546 0 
COMMENT             4813 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BglII" "agatct" 2 0 2 185 0 2344 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 2 1579 0 2015 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 1 2066 1 0 "") 
COMMENT        #35=(CRSite (CStringList) "BmrI" "actggg" 12 11 1 4891 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "BpmI" "ctggag" 23 21 2 2169 1 4862 0 0 "") 
COMMENT        #37=(CRSite (CStringList) "Bpu10I" "cctnagc" 3 6 2 435 0 1943 0 0 "") 
COMMENT        #38=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 4 4327 0 5195 0 5436 1 
COMMENT             5734 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "BsaAI" "yacgtr" 4 0 5 167 0 954 0 1395 0 3319 
COMMENT             0 3788 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "BsaBI" "gatnnnnatc" 6 0 2 2095 0 3296 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 8 45 0 456 0 589 0 1865 0 
COMMENT             1973 0 2887 0 3439 0 4391 0 0 "") 
COMMENT        #42=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 1 4865 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "BsaXI" "acnnnnnctcc" 22 19 5 622 1 1373 1 
COMMENT             1823 1 3827 1 5981 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "BseRI" "gaggag" 17 15 7 646 0 907 1 1394 0 
COMMENT             1699 1 2172 1 2562 1 2623 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "BseYI" "cccagc" 2 6 5 524 0 1157 0 1284 0 
COMMENT             1484 1 5515 0 0 "") 
COMMENT        #46=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 1 1861 1 0 "") 
COMMENT        #47=(CRSite (CStringList) "BsiEI" "cgrycg" 5 0 8 268 0 1165 0 3055 0 
COMMENT             3517 0 4413 0 4562 0 5485 0 5909 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "BsiHKAI" "gwgcwc" 6 0 6 1667 0 2016 0 2650 0 
COMMENT             4263 0 4348 0 5509 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 1 1826 1 0 "") 
COMMENT        #51=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 2 3116 0 3209 1 0 "") 
COMMENT        #52=(CRSite (CStringList) "Bsp1286I" "gdgchc" 6 0 12 202 0 565 0 1656 0 
COMMENT             1667 0 2016 0 2650 0 2790 0 2803 0 3717 0 4263 0 4348 0 5509 0 0 "") 
COMMENT        #53=(CRSite (CStringList) "BspDI" "atcgat" 3 0 4 884 0 2432 0 3027 0 
COMMENT             3290 0 0 "") 
COMMENT        #54=(CRSite (CStringList) "BspEI" "tccgga" 2 0 1 1319 0 0 "") 
COMMENT        #55=(CRSite (CStringList) "BspHI" "tcatga" 2 0 2 4091 0 5099 0 0 "") 
COMMENT        #56=(CRSite (CStringList) "BspMI" "acctgc" 11 15 1 2053 0 0 "") 
COMMENT        #57=(CRSite (CStringList) "BsrBI" "gagcgg" 4 4 4 1995 1 3644 1 4089 1 
COMMENT             5890 1 0 "") 
COMMENT        #58=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 4 983 1 2684 1 4697 0 
COMMENT             4871 1 0 "") 
COMMENT        #59=(CRSite (CStringList) "BsrFI" "rccggy" 2 0 7 465 0 519 0 591 0 1768 
COMMENT             0 2949 0 3683 0 4846 0 0 "") 
COMMENT        #60=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 2 848 0 1749 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 7 3 0 88 0 418 0 420 0 
COMMENT             448 0 3394 0 3479 0 0 "") 
COMMENT        #62=(CRSite (CStringList) "BssSI" "cacgag" 2 6 3 1636 1 4262 0 5646 0 0 
COMMENT             "") #63=(CRSite (CStringList) "BstAPI" "gcannnnntgc" 8 0 0 0 "") 
COMMENT        #64=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 1 2411 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 1 1567 0 0 "") 
COMMENT        #66=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 0 0 "") 
COMMENT        #67=(CRSite (CStringList) "BstYI" "rgatcy" 2 0 14 185 0 681 0 2090 0 
COMMENT             2114 0 2248 0 2344 0 2999 0 3297 0 4284 0 4301 0 5069 0 5081 0 5167 
COMMENT             0 5178 0 0 "") 
COMMENT        #68=(CRSite (CStringList) "BstZ17I" "gtatac" 4 0 0 0 "") 
COMMENT        #69=(CRSite (CStringList) "Bsu36I" "cctnagg" 3 0 0 0 "") 
COMMENT        #70=(CRSite (CStringList) "BtgI" "ccrygg" 2 0 6 387 0 1290 0 2004 0 2741 
COMMENT             0 2813 0 2937 0 0 "") 
COMMENT        #71=(CRSite (CStringList) "BtgZ I" "gcgatg" 17 21 2 2739 1 3783 1 0 "") 
COMMENT        #72=(CRSite (CStringList) "BtsCI" "ggatgnn" 8 6 11 241 0 317 1 355 0 875 
COMMENT             1 1202 1 1482 0 1539 0 1989 1 4485 0 4772 0 4953 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "BtsI" "gcagtg" 9 7 4 369 1 3121 0 4512 0 4532 
COMMENT             1 0 "") #74=(CRSite (CStringList) "ClaI (m)" "gatcgat" 4 6 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "ClaI (nm)" "hatcgatd" 4 0 4 884 0 2432 0 3027 
COMMENT             0 3290 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "DraI" "tttaaa" 4 0 5 1239 0 3256 0 4353 0 
COMMENT             5045 0 5064 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "DraIII" "cacnnngtg" 7 0 1 3791 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "DrdI" "gacnnnnnngtc" 8 0 3 2337 0 3835 0 5717 
COMMENT             0 0 "") 
COMMENT        #79=(CRSite (CStringList) "EaeI" "yggccr" 2 0 9 104 0 491 0 1640 0 1695 
COMMENT             0 1925 0 3052 0 3378 0 4538 0 5980 0 0 "") 
COMMENT        #80=(CRSite (CStringList) "EagI" "cggccg" 2 0 1 3052 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "EarI" "ctcttc" 8 11 8 862 0 1033 0 1261 0 
COMMENT             2398 1 2969 0 3495 1 4132 1 5936 1 0 "") 
COMMENT        #82=(CRSite (CStringList) "EciI" "ggcgga" 18 16 5 2261 0 2761 0 4789 0 
COMMENT             5617 0 5763 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "EcoNI" "cctnnnnnagg" 6 0 1 2403 0 0 "") 
COMMENT        #84=(CRSite (CStringList) "EcoO109I" "rggnccy" 3 0 2 198 0 199 0 0 "") 
COMMENT        #85=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 662 0 2413 0 0 "") 
COMMENT        #86=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 2421 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "FseI" "ggccggcc" 7 0 0 0 "") 
COMMENT        #88=(CRSite (CStringList) "FspI" "tgcgca" 4 0 4 316 0 368 0 3536 0 4708 
COMMENT             0 0 "") 
COMMENT        #89=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 8 592 0 606 0 1868 0 1976 
COMMENT             0 3633 0 3641 0 5579 0 5949 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "HincII" "gtyrac" 4 0 4 928 0 2076 0 3037 0 
COMMENT             3195 0 0 "") 
COMMENT        #91=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2425 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "HpaI" "gttaac" 4 0 2 928 0 3195 0 0 "") 
COMMENT        #93=(CRSite (CStringList) "I-SceI" "twgggataacagggtaat" 10 6 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "KasI" "ggcgcc" 2 0 3 588 0 1864 0 1972 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "MfeI" "caattg" 2 0 1 3204 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 1273 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "MscI" "tggcca" 4 0 3 106 0 1697 0 3380 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "MslI" "caynnnnrtg" 6 0 5 1811 0 2161 0 4160 0 
COMMENT             4519 0 4678 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "MspA1I" "cmgckg" 4 0 11 389 0 628 0 1875 0 
COMMENT              2774 0 2923 0 2939 0 3486 0 4295 0 5236 0 5481 0 5999 0 0 "") 
COMMENT        #101=(CRSite (CStringList) "NaeI" "gccggc" 4 0 4 521 0 593 0 2951 0 3685 
COMMENT              0 0 "") 
COMMENT        #102=(CRSite (CStringList) "NarI" "ggcgcc" 3 0 3 589 0 1865 0 1973 0 0 
COMMENT              "") #103=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 1290 0 0 "") 
COMMENT        #104=(CRSite (CStringList) "NdeI" "catatg" 3 0 0 0 "") 
COMMENT        #105=(CRSite (CStringList) "NgoMIV" "gccggc" 2 0 4 519 0 591 0 2949 0 
COMMENT              3683 0 0 "") 
COMMENT        #106=(CRSite (CStringList) "NheI" "gctagc" 2 0 2 673 0 2146 0 0 "") 
COMMENT        #107=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 3052 0 0 "") 
COMMENT        #108=(CRSite (CStringList) "NruI" "tcgcga" 4 0 1 1018 0 0 "") 
COMMENT        #109=(CRSite (CStringList) "NsiI" "atgcat" 6 0 0 0 "") 
COMMENT        #110=(CRSite (CStringList) "NspI" "rcatgy" 6 0 1 5823 0 0 "") 
COMMENT        #111=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 0 0 "") 
COMMENT        #112=(CRSite (CStringList) "PciI" "acatgt" 2 0 1 5819 0 0 "") 
COMMENT        #113=(CRSite (CStringList) "PflFI" "gacnnngtc" 5 0 1 1837 0 0 "") 
COMMENT        #114=(CRSite (CStringList) "PflMI" "ccannnnntgg" 8 0 2 1291 0 2678 0 0 
COMMENT              "") 
COMMENT        #115=(CRSite (CStringList) "PI-SceI" 
COMMENT              "atctatgtcgggtgcggagaaagaggtaatgaaatggca" 16 12 0 0 "") 
COMMENT        #116=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 0 0 "") 
COMMENT        #117=(CRSite (CStringList) "PmlI" "cacgtg" 4 0 2 954 0 1395 0 0 "") 
COMMENT        #118=(CRSite (CStringList) "PpuMI" "rggwccy" 3 0 0 0 "") 
COMMENT        #119=(CRSite (CStringList) "PshAI" "gacnnnngtc" 6 0 0 0 "") 
COMMENT        #120=(CRSite (CStringList) "PsiI" "ttataa" 4 0 2 3175 0 3916 0 0 "") 
COMMENT        #121=(CRSite (CStringList) "PspOMI" "gggccc" 2 0 1 198 0 0 "") 
COMMENT        #122=(CRSite (CStringList) "PstI" "ctgcag" 6 0 4 195 0 1133 0 1376 0 
COMMENT              2237 0 0 "") 
COMMENT        #123=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 3 1165 0 3517 0 4562 0 0 
COMMENT              "") 
COMMENT        #124=(CRSite (CStringList) "PvuII" "cagctg" 4 0 3 1875 0 3486 0 5999 0 0 
COMMENT              "") #125=(CRSite (CStringList) "RsrII" "cggwccg" 3 0 1 531 0 0 "") 
COMMENT        #126=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 2016 0 0 "") 
COMMENT        #127=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 2 390 0 2940 0 0 "") 
COMMENT        #128=(CRSite (CStringList) "SalI" "gtcgac" 2 0 2 2074 0 3035 0 0 "") 
COMMENT        #129=(CRSite (CStringList) "SapI" "gctcttc" 9 12 2 1261 0 5936 1 0 "") 
COMMENT        #130=(CRSite (CStringList) "SbfI" "cctgcagg" 7 0 1 1376 0 0 "") 
COMMENT        #131=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 4450 0 0 "") 
COMMENT        #132=(CRSite (CStringList) "SexAI" "accwggt" 2 0 0 0 "") 
COMMENT        #133=(CRSite (CStringList) "SfcI" "ctryag" 2 0 9 191 0 1129 0 1372 0 
COMMENT              2025 0 2233 0 3565 0 4685 0 5363 0 5554 0 0 "") 
COMMENT        #134=(CRSite (CStringList) "SfiI" "ggccnnnnnggcc" 9 0 0 0 "") 
COMMENT        #135=(CRSite (CStringList) "SfoI" "ggcgcc" 4 0 3 590 0 1866 0 1974 0 0 
COMMENT              "") 
COMMENT        #136=(CRSite (CStringList) "SgrAI" "crccggyg" 3 0 2 591 0 1768 0 0 "") 
COMMENT        #137=(CRSite (CStringList) "SmaI" "cccggg" 4 0 5 31 0 42 0 3048 0 3444 0 
COMMENT              3455 0 0 "") 
COMMENT        #138=(CRSite (CStringList) "SmlI" "ctyrag" 2 0 6 1966 0 2215 0 4306 0 
COMMENT              5174 0 5451 0 5713 0 0 "") 
COMMENT        #139=(CRSite (CStringList) "SnaBI" "tacgta" 4 0 2 167 0 3319 0 0 "") 
COMMENT        #140=(CRSite (CStringList) "SpeI" "actagt" 2 0 0 0 "") 
COMMENT        #141=(CRSite (CStringList) "SphI" "gcatgc" 6 0 0 0 "") 
COMMENT        #142=(CRSite (CStringList) "SrfI" "gcccgggc" 5 0 4 31 0 42 0 3444 0 3455 
COMMENT              0 0 "") 
COMMENT        #143=(CRSite (CStringList) "SspI" "aatatt" 4 0 2 3996 0 4126 0 0 "") 
COMMENT        #144=(CRSite (CStringList) "StuI" "aggcct" 4 0 2 1502 0 1595 0 0 "") 
COMMENT        #145=(CRSite (CStringList) "StyI" "ccwwgg" 2 0 7 497 0 938 0 1185 0 1290 
COMMENT              0 1503 0 1563 0 2845 0 0 "") 
COMMENT        #146=(CRSite (CStringList) "SwaI" "atttaaat" 5 0 0 0 "") 
COMMENT        #147=(CRSite (CStringList) "Tth111I" "gacnnngtc" 5 0 1 1837 0 0 "") 
COMMENT        #148=(CRSite (CStringList) "XbaI" "tctaga" 2 0 0 0 "") 
COMMENT        #149=(CRSite (CStringList) "XcmI" "ccannnnnnnnntgg" 9 0 1 1768 0 0 "") 
COMMENT        #150=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 0 0 "") 
COMMENT        #151=(CRSite (CStringList) "XmaI" "cccggg" 2 0 5 29 0 40 0 3046 0 3442 0 
COMMENT              3453 0 0 "") 
COMMENT        #152=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 2 2407 0 4331 0 0 "")) 
COMMENT       (CObList 
COMMENT        #153=(CFSignal (CObList) "AmpR" 21 0 0 4143 5000 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "4199..5056") 
COMMENT        #154=(CFSignal (CObList) "5' ITR" 21 0 0 1 167 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "57..223") 
COMMENT        #155=(CFSignal (CObList) "3' ITR" 21 0 0 3318 3484 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "3374..3540") 
COMMENT        #156=(CFSignal (CObList) "loxP" 21 0 0 784 817 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "840..873") 
COMMENT        #157=(CFSignal (CObList) "loxP" 21 0 0 2361 2394 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "2417..2450") 
COMMENT        #158=(CFSignal (CObList) "loxP2272" 21 0 0 688 721 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "744..777") 
COMMENT        #159=(CFSignal (CObList) "loxP2272" 21 0 0 2257 2290 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "2313..2346") 
COMMENT        #160=(CFSignal (CObList) "NES-jRGECO1b" 21 0 1 828 2240 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "complement(884..2296)") 
COMMENT        #161=(CFSignal (CObList) "SV40 polyadenylation signal" 25 0 0 3058 3289 
COMMENT              0 (CStringList) (CStringList) 1 1 1 1 "3114..3345") 
COMMENT        #162=(CFSignal (CObList) "synapsin promoter" 29 0 0 190 662 0 
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COMMENT                             "ACNNNNGTAYCNNNNNNNNNNNNN" 
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COMMENT                             "GAAGACNNNNNNN" "CTTCTGNNNNNNN") 
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COMMENT                             "CGANNNNNNTGCNNNNNNNNNNNNN" 
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COMMENT                              "BciVI: 5 sites") 12 11 "GTATCCNNNNNNN" 
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COMMENT                              "BclI: 2 sites") 1 5 "TGATCA" "ACTAGT") 
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COMMENT                              "BfuAI: 1 site") 10 14 "ACCTGCNNNNNNNNN" 
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COMMENT                              "BglI: 4 sites") 7 4 "GCCNNNNNGGC" "CGGNNNNNCCG") 
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COMMENT                              "BglII: 2 sites") 1 5 "AGATCT" "TCTAGA") 
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COMMENT                              "BlpI: 2 sites") 2 5 "GCTNAGC" "CGANTCG") 
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COMMENT                             "CACGTC" "GTGCAG") 
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COMMENT                              "BmrI: 1 site") 11 10 "ACTGGGNNNNNN" 
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COMMENT                              "BpmI: 2 sites") 22 20 "CTGGAGNNNNNNNNNNNNNNNNN" 
COMMENT                             "GACCTCNNNNNNNNNNNNNNNNN") 
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COMMENT                              "Bpu10I: 2 sites") 2 5 "CCTNAGC" "GGANTCG") 
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COMMENT                                             " N3: 5436 (on complementary strand)" 
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COMMENT                              "BpuEI: 4 sites") 22 20 "CTTGAGNNNNNNNNNNNNNNNNN" 
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COMMENT                              "BsaAI: 5 sites") 3 3 "YACGTR" "RTGCAY") 
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COMMENT                              "BsaBI: 2 sites") 5 5 "GATNNNNATC" "CTANNNNTAG") 
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COMMENT                              "BsaHI: 8 sites") 2 4 "GRCGYC" "CYGCRG") 
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COMMENT                              "BsaI: 1 site") 7 11 "GGTCTCNNNNNN" "CCAGAGNNNNNN") 
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COMMENT                                             " N1: 622 (on complementary strand)" 
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COMMENT                                       #430=(CLinePar 
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COMMENT                                             " N2: 1373 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #431=(CLinePar 
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COMMENT                                             " N3: 1823 (on complementary strand)" 
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COMMENT                                             " N4: 3827 (on complementary strand)" 
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COMMENT                              "BsaXI: 5 sites") 21 18 "ACNNNNNCTCCNNNNNNNNNNN" 
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COMMENT                                       #437=(CLinePar 
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COMMENT                                             " N2: 907 (on complementary strand)" 
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COMMENT                                       #438=(CLinePar 
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COMMENT                                       #439=(CLinePar 
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COMMENT                                             " N4: 1699 (on complementary strand)" 
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COMMENT                                       #440=(CLinePar 
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COMMENT                                             " N5: 2172 (on complementary strand)" 
COMMENT                                             1) 
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COMMENT                                              191) 
COMMENT                                             " N6: 2562 (on complementary strand)" 
COMMENT                                             1) 
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COMMENT                              "BseRI: 7 sites") 16 14 "GAGGAGNNNNNNNNNNN" 
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COMMENT                              "MscI: 3 sites") 3 3 "TGGCCA" "ACCGGT") 
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COMMENT                              "MslI: 5 sites") 5 5 "CAYNNNNRTG" "GTRNNNNYAC") 
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COMMENT                              "MspA1I: 11 sites") 3 3 "CMGCKG" "GKCGMC") 
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COMMENT                              "NaeI: 4 sites") 3 3 "GCCGGC" "CGGCCG") 
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COMMENT                              "NarI: 3 sites") 2 4 "GGCGCC" "CCGCGG") 
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COMMENT                              "NcoI: 1 site") 1 5 "CCATGG" "GGTACC") 
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COMMENT                              "NheI: 2 sites") 1 5 "GCTAGC" "CGATCG") 
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COMMENT                              "NruI: 1 site") 3 3 "TCGCGA" "AGCGCT") 
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COMMENT                              "NspI: 1 site") 5 1 "RCATGY" "YGTACR") 
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COMMENT                              "PciI: 1 site") 1 5 "ACATGT" "TGTACA") 
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COMMENT                              "PflMI: 2 sites") 7 4 "CCANNNNNTGG" "GGTNNNNNACC") 
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COMMENT                             "ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGGCA" 
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COMMENT                              "PmlI: 2 sites") 3 3 "CACGTG" "GTGCAC") 
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COMMENT                              "PsiI: 2 sites") 3 3 "TTATAA" "AATATT") 
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COMMENT                              "PspOMI: 1 site") 1 5 "GGGCCC" "CCCGGG") 
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COMMENT                              "PstI: 4 sites") 5 1 "CTGCAG" "GACGTC") 
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COMMENT                              "PvuI: 3 sites") 4 2 "CGATCG" "GCTAGC") 
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COMMENT                              "PvuII: 3 sites") 3 3 "CAGCTG" "GTCGAC") 
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COMMENT                              "RsrII: 1 site") 2 5 "CGGWCCG" "GCCWGGC") 
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COMMENT                              "SacI: 1 site") 5 1 "GAGCTC" "CTCGAG") 
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COMMENT                              "SacII: 2 sites") 4 2 "CCGCGG" "GGCGCC") 
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COMMENT                              "SalI: 2 sites") 1 5 "GTCGAC" "CAGCTG") 
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COMMENT                                             " N2: 5936 (on complementary strand)" 
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COMMENT                             "GCTCTTCNNNNN" "CGAGAAGNNNNN") 
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COMMENT                              "SbfI: 1 site") 6 2 "CCTGCAGG" "GGACGTCC") 
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COMMENT                              "SfcI: 9 sites") 1 5 "CTRYAG" "GAYRTC") 
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FEATURES             Location/Qualifiers
     misc_feature    4143..5000
                     /vntifkey="21"
                     /label=AmpR
                     /note="AmpR"
     misc_feature    1..167
                     /vntifkey="21"
                     /label=5'\ITR
                     /note="5' ITR"
     misc_feature    3318..3484
                     /vntifkey="21"
                     /label=3'\ITR
                     /note="3' ITR"
     misc_feature    784..817
                     /vntifkey="21"
                     /label=loxP
                     /note="loxP"
     misc_feature    2361..2394
                     /vntifkey="21"
                     /label=loxP
                     /note="loxP"
     misc_feature    688..721
                     /vntifkey="21"
                     /label=loxP2272
                     /note="loxP2272"
     misc_feature    2257..2290
                     /vntifkey="21"
                     /label=loxP2272
                     /note="loxP2272"
     misc_feature    complement(828..2240)
                     /vntifkey="21"
                     /label=NES-jRGECO1b
                     /note="NES-jRGECO1b"
     polyA_signal    3058..3289
                     /vntifkey="25"
                     /label=SV40\polyadenylation\signal
                     /note="SV40 polyadenylation signal"
     promoter        190..662
                     /vntifkey="29"
                     /label=synapsin\promoter
                     /note="synapsin promoter"
     misc_feature    2436..3024
                     /vntifkey="21"
                     /label=WPRE
                     /note="WPRE"
BASE COUNT     1298 a      1669 c      1520 g      1512 t 
ORIGIN
        1 tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc cgggcgtcgg gcgacctttg 
       61 gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta 
      121 ggggttcctt gtagttaatg attaacccgc catgctactt atctacgtag ccatgctcta 
      181 ggaagatctc tgcagagggc cctgcgtatg agtgcaagtg ggttttagga ccaggatgag 
      241 gcggggtggg ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc 
      301 cattccccaa attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg 
      361 cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc 
      421 gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac 
      481 tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca 
      541 cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc 
      601 agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc 
      661 gaattcaagc tgctagcaag gatccccata acttcgtata aagtatccta tacgaagtta 
      721 tatcaaaata ggaagaccaa tgcttcacca tcgacccgaa ttgccaagca tcaccatcga 
      781 cccataactt cgtataatgt atgctatacg aagttatact agagctttca cttcgctgtc 
      841 atcatttgta caaactcttc gtagtttacc tgaccatccc catcgatgtc tgctaccctg 
      901 atcatttcat caacctcctc atctgttaac ttctctccaa ggtctgtcat cacgtggcga 
      961 agctctgctg cgccgatgta gccattgccg tccttatcaa acacgcggaa cgcttcgcga 
     1021 atttcctctt cactgtctgt gtcattcatt tttcttgcca tcatcgtcag gaactcaggg 
     1081 aagtcgaatg tgccgtcacc gtcggcatct acttcattga tcatgtcctg cagctctgct 
     1141 tctgtggggt tctgccccag cgatcgcatc accgtcccaa gctccttggt tgttatcgtc 
     1201 ccatccccgt ccttgtcaaa gcaggagaaa gcctctttaa attctgcgat ctgctcttca 
     1261 gtcagttggt cacgcgtagc ctcccagccc atggtcttct tctgcattac ggggccgtcc 
     1321 ggagggaagt tggtgccgcg cagcttcacc ttgtagatga atacgccgtc ctgcagggag 
     1381 gagtcctggt tcacgtgaat aatgccgccg tcctcgaagt tcatcacgcg ctcccacctg 
     1441 aagccctcgg ggaaggacag cttgaagtag tcggggatgt cggctgggtg cttaatgtag 
     1501 gccttggagc cgtacatgaa ctgaggggac aggatgtccc aggcgaaggg cagggggcca 
     1561 cccttggtca ccttcagctt agcggtctga aaggcctcgt aggggcggcc ctcgccctcg 
     1621 ccctcgatct cgaactcgtg gccgttcacg gagccctcca tgtgcacctt gaagcgcatg 
     1681 aactccttga tgatggccat gttatcctcc tcgcccttgc tcaccagact cccgcctgta 
     1741 cctcccttgt acagctcgtc catgccgccg gtggagtggc ggccctcggc acattcgcac 
     1801 tgttccacga tggtgtagtc ctcgttgtgg gagacgatgt ccaacttgat gtccaccttg 
     1861 taggcgccgg gcagctgcac gggcttcttg gccttgtagg tagtcttgac ctcggcggcg 
     1921 tagtggccgc cgtccttcag cctcagcccc ttcttgatct cgctcttaag ggcgccgtcc 
     1981 tcggggtaca tccgctcgga aaccacgggt gagctcagcc gacctatagc tctgactgcg 
     2041 tgacctgcct tattccactt acgacgtgat gagtcgacca ttgttgcgag atccttatcg 
     2101 tcatcgtcgt acagatcccg acccatttgc tgtccaccag tcatgctagc cataccatga 
     2161 tgatgatgat gatgagaacc tcctccagtc ttgttaatat caagtccagc caacttaaga 
     2221 gcaagctcgt tctgcagcat ggtggcggat cctagtataa cttcgtatag gatactttat 
     2281 acgaagttat cattgggatt cttcctattt tgatccaagc atcaccatcg accctctagt 
     2341 ccagatctca ccatcgaccc ataacttcgt atagcataca ttatacgaag ttatgtccct 
     2401 cgaagaggtt cgaattcgat atcaagctta tcgataatca acctctggat tacaaaattt 
     2461 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg 
     2521 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt 
     2581 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 
     2641 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 
     2701 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg 
     2761 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt 
     2821 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc 
     2881 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg 
     2941 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga 
     3001 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgacccggg cggccgcttc 
     3061 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa 
     3121 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct 
     3181 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga 
     3241 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc 
     3301 ttcctagagc atggctacgt agataagtag catggcgggt taatcattaa ctacaaggaa 
     3361 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg 
     3421 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg 
     3481 cgcagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 
     3541 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 
     3601 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 
     3661 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 
     3721 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 
     3781 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 
     3841 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 
     3901 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 
     3961 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca 
     4021 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 
     4081 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 
     4141 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 
     4201 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 
     4261 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 
     4321 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 
     4381 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 
     4441 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 
     4501 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 
     4561 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 
     4621 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 
     4681 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 
     4741 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 
     4801 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 
     4861 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 
     4921 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 
     4981 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 
     5041 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 
     5101 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 
     5161 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 
     5221 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 
     5281 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt 
     5341 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 
     5401 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 
     5461 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 
     5521 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 
     5581 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 
     5641 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 
     5701 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 
     5761 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 
     5821 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 
     5881 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 
     5941 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagc 
//
