LOCUS       pAAV.Syn.NES-jRGECO1b.WPRE.SV40(p3846)        5684 bp    DNA     circular     18-JUN-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|737034320|
COMMENT     VNTDBDATE|737034320|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV.Syn.NES-jRGECO1b.WPRE.SV40(p3846)|
COMMENT     VNTAUTHORNAME|Erich Kurtz|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAAV.Syn.NES-jRGECO1b.WPRE.SV40(p3846)" 0 5684 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "AatI" "AatII" "Acc65I" "AccI" "AclI" "Acu I" "AfeI" 
COMMENT         "AflII" "AflIII" "AgeI" "AhdI" "AleI" "AlwNI" "ApaI" "ApaLI" "AscI" 
COMMENT         "AseI" "AsiSI" "AvaI" "AvrII" "BaeI" "BamHI" "BanI" "BanII" "BbsI" 
COMMENT         "BbvCI" "BcgI" "BciVI" "BclI" "BfrBI" "BfuAI" "BglI" "BglII" "BlpI" 
COMMENT         "BmgBI" "BmrI" "BpmI" "Bpu10I" "BpuEI" "BsaAI" "BsaBI" "BsaHI" "BsaI" 
COMMENT         "BsaXI" "BseRI" "BseYI" "BsgI" "BsiEI" "BsiHKAI" "BsiWI" "BsmBI" "BsmI" 
COMMENT         "Bsp1286I" "BspDI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrFI" 
COMMENT         "BsrGI" "BssHII" "BssSI" "BstAPI" "BstBI" "BstEII" "BstXI" "BstYI" 
COMMENT         "BstZ17I" "Bsu36I" "BtgI" "BtgZ I" "BtsCI" "BtsI" "ClaI (m)" 
COMMENT         "ClaI (nm)" "DraI" "DraIII" "DrdI" "EaeI" "EagI" "EarI" "EciI" "EcoNI" 
COMMENT         "EcoO109I" "EcoRI" "EcoRV" "FseI" "FspI" "HaeII" "HincII" "HindIII" 
COMMENT         "HpaI" "I-SceI" "KasI" "KpnI" "MfeI" "MluI" "MscI" "MslI" "MspA1I" 
COMMENT         "NaeI" "NarI" "NcoI" "NdeI" "NgoMIV" "NheI" "NotI" "NruI" "NsiI" "NspI" 
COMMENT         "PacI" "PciI" "PflFI" "PflMI" "PI-SceI" "PmeI" "PmlI" "PpuMI" "PshAI" 
COMMENT         "PsiI" "PspOMI" "PstI" "PvuI" "PvuII" "RsrII" "SacI" "SacII" "SalI" 
COMMENT         "SapI" "SbfI" "ScaI" "SexAI" "SfcI" "SfiI" "SfoI" "SgrAI" "SmaI" "SmlI" 
COMMENT         "SnaBI" "SpeI" "SphI" "SrfI" "SspI" "StuI" "StyI" "SwaI" "Tth111I" 
COMMENT         "XbaI" "XcmI" "XhoI" "XmaI" "XmnI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 5684 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "AatI" "aggcct" 4 0 2 1342 0 1435 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AatII" "gacgtc" 6 0 2 459 0 2575 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "AccI" "gtmkac" 3 0 2 860 0 2721 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "AclI" "aacgtt" 3 0 2 4014 0 4387 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "Acu I" "ctgaag" 23 21 8 364 1 463 0 1018 0 
COMMENT            1381 0 1479 1 1697 0 3950 0 4962 1 0 "") 
COMMENT        #6=(CRSite (CStringList) "AfeI" "agcgct" 4 0 1 604 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "AflII" "cttaag" 2 0 2 718 0 967 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "AflIII" "acrygt" 2 0 2 1660 0 5504 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "AgeI" "accggt" 2 0 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "AhdI" "gacnnnnngtc" 7 0 4 59 0 1404 0 3113 0 
COMMENT             4616 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "AleI" "cacnnnngtg" 6 0 1 1126 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AlwNI" "cagnnnctg" 7 0 1 5095 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 202 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 4 1270 0 2331 0 3944 0 
COMMENT             5190 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "AseI" "attaat" 3 0 2 4441 0 5676 0 0 "") 
COMMENT        #17=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 1 1774 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 7 29 0 40 0 952 0 1486 0 
COMMENT             2731 0 3127 0 3138 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "BaeI" "acnnnngtayc" 24 19 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 681 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 7 252 0 588 0 961 0 1069 0 
COMMENT             1600 0 3432 0 4663 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "BanII" "grgcyc" 6 0 5 202 0 925 0 1285 0 2475 
COMMENT             0 3402 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "BbsI" "gaagac" 9 13 2 1645 0 2650 1 0 "") 
COMMENT        #25=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 2 435 0 991 1 0 "") 
COMMENT        #26=(CRSite (CStringList) "BcgI" "cgannnnnntgc" 25 23 3 380 0 796 1 4078 
COMMENT             1 0 "") 
COMMENT        #27=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 3 2191 1 3779 0 5306 0 
COMMENT             0 "") 
COMMENT        #28=(CRSite (CStringList) "BclI" "tgatca" 2 0 2 1814 0 2033 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BfuAI" "acctgc" 11 15 1 880 1 0 "") 
COMMENT        #31=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 4 31 0 3143 0 3231 0 
COMMENT             4498 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BglII" "agatct" 2 0 1 185 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 2 919 0 1355 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 1 871 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "BmrI" "actggg" 12 11 1 4576 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "BpmI" "ctggag" 23 21 2 770 0 4547 0 0 "") 
COMMENT        #37=(CRSite (CStringList) "Bpu10I" "cctnagc" 3 6 2 435 0 991 1 0 "") 
COMMENT        #38=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 4 4012 0 4880 0 5121 1 
COMMENT             5419 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "BsaAI" "yacgtr" 4 0 5 167 0 1542 0 1983 0 
COMMENT             3004 0 3473 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "BsaBI" "gatnnnnatc" 6 0 2 842 0 2981 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 8 45 0 456 0 589 0 962 0 
COMMENT             1070 0 2572 0 3124 0 4076 0 0 "") 
COMMENT        #42=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 1 4550 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "BsaXI" "acnnnnnctcc" 22 19 5 622 1 1117 0 
COMMENT             1567 0 3512 1 5666 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "BseRI" "gaggag" 17 15 7 646 0 767 0 1240 0 
COMMENT             1545 1 2032 0 2247 1 2308 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "BseYI" "cccagc" 2 6 5 524 0 1449 0 1649 1 
COMMENT             1776 1 5200 0 0 "") 
COMMENT        #46=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 1 1078 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "BsiEI" "cgrycg" 5 0 8 268 0 1774 0 2740 0 
COMMENT             3202 0 4098 0 4247 0 5170 0 5594 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "BsiHKAI" "gwgcwc" 6 0 6 925 0 1274 0 2335 0 
COMMENT             3948 0 4033 0 5194 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 1 1107 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 2 2801 0 2894 1 0 "") 
COMMENT        #52=(CRSite (CStringList) "Bsp1286I" "gdgchc" 6 0 12 202 0 565 0 925 0 
COMMENT             1274 0 1285 0 2335 0 2475 0 2488 0 3402 0 3948 0 4033 0 5194 0 0 "") 
COMMENT        #53=(CRSite (CStringList) "BspDI" "atcgat" 3 0 4 2051 0 2117 0 2712 0 
COMMENT             2975 0 0 "") 
COMMENT        #54=(CRSite (CStringList) "BspEI" "tccgga" 2 0 1 1614 0 0 "") 
COMMENT        #55=(CRSite (CStringList) "BspHI" "tcatga" 2 0 2 3776 0 4784 0 0 "") 
COMMENT        #56=(CRSite (CStringList) "BspMI" "acctgc" 11 15 1 880 1 0 "") 
COMMENT        #57=(CRSite (CStringList) "BsrBI" "gagcgg" 4 4 4 942 0 3329 1 3774 1 
COMMENT             5575 1 0 "") 
COMMENT        #58=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 4 1956 0 2369 1 4382 0 
COMMENT             4556 1 0 "") 
COMMENT        #59=(CRSite (CStringList) "BsrFI" "rccggy" 2 0 7 465 0 519 0 591 0 1165 
COMMENT             0 2634 0 3368 0 4531 0 0 "") 
COMMENT        #60=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 2 1184 0 2085 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 7 3 0 88 0 418 0 420 0 
COMMENT             448 0 3079 0 3164 0 0 "") 
COMMENT        #62=(CRSite (CStringList) "BssSI" "cacgag" 2 6 3 1297 0 3947 0 5331 0 0 
COMMENT             "") #63=(CRSite (CStringList) "BstAPI" "gcannnnntgc" 8 0 0 0 "") 
COMMENT        #64=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 1 1365 0 0 "") 
COMMENT        #66=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 0 0 "") 
COMMENT        #67=(CRSite (CStringList) "BstYI" "rgatcy" 2 0 12 185 0 681 0 819 0 843 
COMMENT             0 2684 0 2982 0 3969 0 3986 0 4754 0 4766 0 4852 0 4863 0 0 "") 
COMMENT        #68=(CRSite (CStringList) "BstZ17I" "gtatac" 4 0 0 0 "") 
COMMENT        #69=(CRSite (CStringList) "Bsu36I" "cctnagg" 3 0 0 0 "") 
COMMENT        #70=(CRSite (CStringList) "BtgI" "ccrygg" 2 0 6 387 0 929 0 1643 0 2426 
COMMENT             0 2498 0 2622 0 0 "") 
COMMENT        #71=(CRSite (CStringList) "BtgZ I" "gcgatg" 17 21 2 2424 1 3468 1 0 "") 
COMMENT        #72=(CRSite (CStringList) "BtsCI" "ggatgnn" 8 6 11 241 0 317 1 355 0 950 
COMMENT             0 1400 1 1457 1 1737 0 2064 0 4170 0 4457 0 4638 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "BtsI" "gcagtg" 9 7 4 369 1 2806 0 4197 0 4217 
COMMENT             1 0 "") #74=(CRSite (CStringList) "ClaI (m)" "gatcgat" 4 6 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "ClaI (nm)" "hatcgatd" 4 0 4 2051 0 2117 0 
COMMENT             2712 0 2975 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "DraI" "tttaaa" 4 0 5 1698 0 2941 0 4038 0 
COMMENT             4730 0 4749 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "DraIII" "cacnnngtg" 7 0 1 3476 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "DrdI" "gacnnnnnngtc" 8 0 2 3520 0 5402 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "EaeI" "yggccr" 2 0 9 104 0 491 0 1008 0 1238 
COMMENT             0 1293 0 2737 0 3063 0 4223 0 5665 0 0 "") 
COMMENT        #80=(CRSite (CStringList) "EagI" "cggccg" 2 0 1 2737 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "EarI" "ctcttc" 8 11 7 1673 1 1901 1 2072 1 
COMMENT             2654 0 3180 1 3817 1 5621 1 0 "") 
COMMENT        #82=(CRSite (CStringList) "EciI" "ggcgga" 18 16 4 2446 0 4474 0 5302 0 
COMMENT             5448 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "EcoNI" "cctnnnnnagg" 6 0 0 0 "") 
COMMENT        #84=(CRSite (CStringList) "EcoO109I" "rggnccy" 3 0 2 198 0 199 0 0 "") 
COMMENT        #85=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 662 0 0 "") 
COMMENT        #86=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "FseI" "ggccggcc" 7 0 0 0 "") 
COMMENT        #88=(CRSite (CStringList) "FspI" "tgcgca" 4 0 4 316 0 368 0 3221 0 4393 
COMMENT             0 0 "") 
COMMENT        #89=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 8 592 0 606 0 965 0 1073 
COMMENT             0 3318 0 3326 0 5264 0 5634 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "HincII" "gtyrac" 4 0 4 861 0 2009 0 2722 0 
COMMENT             2880 0 0 "") 
COMMENT        #91=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2110 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "HpaI" "gttaac" 4 0 2 2009 0 2880 0 0 "") 
COMMENT        #93=(CRSite (CStringList) "I-SceI" "twgggataacagggtaat" 10 6 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "KasI" "ggcgcc" 2 0 3 588 0 961 0 1069 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "MfeI" "caattg" 2 0 1 2889 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 1660 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "MscI" "tggcca" 4 0 3 106 0 1240 0 3065 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "MslI" "caynnnnrtg" 6 0 5 776 0 1126 0 3845 0 
COMMENT             4204 0 4363 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "MspA1I" "cmgckg" 4 0 11 389 0 628 0 1062 0 
COMMENT              2459 0 2608 0 2624 0 3171 0 3980 0 4921 0 5166 0 5684 0 0 "") 
COMMENT        #101=(CRSite (CStringList) "NaeI" "gccggc" 4 0 4 521 0 593 0 2636 0 3370 
COMMENT              0 0 "") 
COMMENT        #102=(CRSite (CStringList) "NarI" "ggcgcc" 3 0 3 589 0 962 0 1070 0 0 "") 
COMMENT        #103=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 1643 0 0 "") 
COMMENT        #104=(CRSite (CStringList) "NdeI" "catatg" 3 0 0 0 "") 
COMMENT        #105=(CRSite (CStringList) "NgoMIV" "gccggc" 2 0 4 519 0 591 0 2634 0 
COMMENT              3368 0 0 "") 
COMMENT        #106=(CRSite (CStringList) "NheI" "gctagc" 2 0 2 673 0 787 0 0 "") 
COMMENT        #107=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 2737 0 0 "") 
COMMENT        #108=(CRSite (CStringList) "NruI" "tcgcga" 4 0 1 1919 0 0 "") 
COMMENT        #109=(CRSite (CStringList) "NsiI" "atgcat" 6 0 0 0 "") 
COMMENT        #110=(CRSite (CStringList) "NspI" "rcatgy" 6 0 1 5508 0 0 "") 
COMMENT        #111=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 0 0 "") 
COMMENT        #112=(CRSite (CStringList) "PciI" "acatgt" 2 0 1 5504 0 0 "") 
COMMENT        #113=(CRSite (CStringList) "PflFI" "gacnnngtc" 5 0 1 1099 0 0 "") 
COMMENT        #114=(CRSite (CStringList) "PflMI" "ccannnnntgg" 8 0 2 1649 0 2363 0 0 
COMMENT              "") 
COMMENT        #115=(CRSite (CStringList) "PI-SceI" 
COMMENT              "atctatgtcgggtgcggagaaagaggtaatgaaatggca" 16 12 0 0 "") 
COMMENT        #116=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 0 0 "") 
COMMENT        #117=(CRSite (CStringList) "PmlI" "cacgtg" 4 0 2 1542 0 1983 0 0 "") 
COMMENT        #118=(CRSite (CStringList) "PpuMI" "rggwccy" 3 0 0 0 "") 
COMMENT        #119=(CRSite (CStringList) "PshAI" "gacnnnngtc" 6 0 0 0 "") 
COMMENT        #120=(CRSite (CStringList) "PsiI" "ttataa" 4 0 2 2860 0 3601 0 0 "") 
COMMENT        #121=(CRSite (CStringList) "PspOMI" "gggccc" 2 0 1 198 0 0 "") 
COMMENT        #122=(CRSite (CStringList) "PstI" "ctgcag" 6 0 4 195 0 704 0 1565 0 1808 
COMMENT              0 0 "") 
COMMENT        #123=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 3 1774 0 3202 0 4247 0 0 
COMMENT              "") 
COMMENT        #124=(CRSite (CStringList) "PvuII" "cagctg" 4 0 3 1062 0 3171 0 5684 0 0 
COMMENT              "") #125=(CRSite (CStringList) "RsrII" "cggwccg" 3 0 1 531 0 0 "") 
COMMENT        #126=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 925 0 0 "") 
COMMENT        #127=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 2 390 0 2625 0 0 "") 
COMMENT        #128=(CRSite (CStringList) "SalI" "gtcgac" 2 0 2 859 0 2720 0 0 "") 
COMMENT        #129=(CRSite (CStringList) "SapI" "gctcttc" 9 12 2 1673 1 5621 1 0 "") 
COMMENT        #130=(CRSite (CStringList) "SbfI" "cctgcagg" 7 0 1 1565 0 0 "") 
COMMENT        #131=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 4135 0 0 "") 
COMMENT        #132=(CRSite (CStringList) "SexAI" "accwggt" 2 0 0 0 "") 
COMMENT        #133=(CRSite (CStringList) "SfcI" "ctryag" 2 0 9 191 0 700 0 908 0 1561 
COMMENT              0 1804 0 3250 0 4370 0 5048 0 5239 0 0 "") 
COMMENT        #134=(CRSite (CStringList) "SfiI" "ggccnnnnnggcc" 9 0 0 0 "") 
COMMENT        #135=(CRSite (CStringList) "SfoI" "ggcgcc" 4 0 3 590 0 963 0 1071 0 0 "") 
COMMENT        #136=(CRSite (CStringList) "SgrAI" "crccggyg" 3 0 2 591 0 1165 0 0 "") 
COMMENT        #137=(CRSite (CStringList) "SmaI" "cccggg" 4 0 5 31 0 42 0 2733 0 3129 0 
COMMENT              3140 0 0 "") 
COMMENT        #138=(CRSite (CStringList) "SmlI" "ctyrag" 2 0 6 718 0 967 0 3991 0 4859 
COMMENT              0 5136 0 5398 0 0 "") 
COMMENT        #139=(CRSite (CStringList) "SnaBI" "tacgta" 4 0 2 167 0 3004 0 0 "") 
COMMENT        #140=(CRSite (CStringList) "SpeI" "actagt" 2 0 0 0 "") 
COMMENT        #141=(CRSite (CStringList) "SphI" "gcatgc" 6 0 0 0 "") 
COMMENT        #142=(CRSite (CStringList) "SrfI" "gcccgggc" 5 0 4 31 0 42 0 3129 0 3140 
COMMENT              0 0 "") 
COMMENT        #143=(CRSite (CStringList) "SspI" "aatatt" 4 0 2 3681 0 3811 0 0 "") 
COMMENT        #144=(CRSite (CStringList) "StuI" "aggcct" 4 0 2 1342 0 1435 0 0 "") 
COMMENT        #145=(CRSite (CStringList) "StyI" "ccwwgg" 2 0 7 497 0 1370 0 1430 0 
COMMENT              1643 0 1748 0 1995 0 2530 0 0 "") 
COMMENT        #146=(CRSite (CStringList) "SwaI" "atttaaat" 5 0 0 0 "") 
COMMENT        #147=(CRSite (CStringList) "Tth111I" "gacnnngtc" 5 0 1 1099 0 0 "") 
COMMENT        #148=(CRSite (CStringList) "XbaI" "tctaga" 2 0 0 0 "") 
COMMENT        #149=(CRSite (CStringList) "XcmI" "ccannnnnnnnntgg" 9 0 1 1170 0 0 "") 
COMMENT        #150=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 0 0 "") 
COMMENT        #151=(CRSite (CStringList) "XmaI" "cccggg" 2 0 5 29 0 40 0 2731 0 3127 0 
COMMENT              3138 0 0 "") 
COMMENT        #152=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 1 4016 0 0 "")) 
COMMENT       (CObList 
COMMENT        #153=(CFSignal (CObList) "AmpR" 21 0 0 3828 4685 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #154=(CFSignal (CObList) "5' ITR" 21 0 0 1 167 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #155=(CFSignal (CObList) "3' ITR" 21 0 0 3003 3169 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #156=(CFSignal (CObList) "NES-jRGECO1b" 21 0 0 696 2108 0 (CStringList) 
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COMMENT        #157=(CFSignal (CObList) "SV40 polyadenylation signal" 25 0 0 2743 2974 
COMMENT              0 (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #158=(CFSignal (CObList) "synapsin promoter" 29 0 0 190 661 0 
COMMENT              (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #159=(CFSignal (CObList) "WPRE" 21 0 0 2121 2709 0 (CStringList) 
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COMMENT                             "CCTNAGC" "GGANTCG") 
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COMMENT                              "BsaAI: 5 sites") 3 3 "YACGTR" "RTGCAY") 
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COMMENT                              "BsaHI: 8 sites") 2 4 "GRCGYC" "CYGCRG") 
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COMMENT                              "BsaI: 1 site") 7 11 "GGTCTCNNNNNN" "CCAGAGNNNNNN") 
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COMMENT                              "BsaXI: 5 sites") 21 18 "ACNNNNNCTCCNNNNNNNNNNN" 
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COMMENT                              "BseRI: 7 sites") 16 14 "GAGGAGNNNNNNNNNNN" 
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COMMENT                              "BseYI: 5 sites") 1 5 "CCCAGC" "GGGTCG") 
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COMMENT                              "BsmBI: 1 site") 7 11 "CGTCTCNNNNNN" 
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COMMENT                                             " N7: 5621 (on complementary strand)" 
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COMMENT                             "CTCTTCNNNNN" "GAGAAGNNNNN") 
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COMMENT                              "EciI: 4 sites") 17 15 "GGCGGANNNNNNNNNNNN" 
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COMMENT                              "EcoO109I: 2 sites") 2 5 "RGGNCCY" "YCCNGGR") 
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COMMENT                              "FspI: 4 sites") 3 3 "TGCGCA" "ACGCGT") 
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COMMENT                              "HincII: 4 sites") 3 3 "GTYRAC" "CARYTG") 
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COMMENT                              "HindIII: 1 site") 1 5 "AAGCTT" "TTCGAA") 
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COMMENT                              "HpaI: 2 sites") 3 3 "GTTAAC" "CAATTG") 
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COMMENT                              "KasI: 3 sites") 1 5 "GGCGCC" "CCGCGG") 
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COMMENT                              "MfeI: 1 site") 1 5 "CAATTG" "GTTAAC") 
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COMMENT                              "MluI: 1 site") 1 5 "ACGCGT" "TGCGCA") 
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COMMENT                              "MscI: 3 sites") 3 3 "TGGCCA" "ACCGGT") 
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COMMENT                              "MslI: 5 sites") 5 5 "CAYNNNNRTG" "GTRNNNNYAC") 
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COMMENT                              "MspA1I: 11 sites") 3 3 "CMGCKG" "GKCGMC") 
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COMMENT                              "NaeI: 4 sites") 3 3 "GCCGGC" "CGGCCG") 
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COMMENT                              "NarI: 3 sites") 2 4 "GGCGCC" "CCGCGG") 
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COMMENT                              "NcoI: 1 site") 1 5 "CCATGG" "GGTACC") 
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COMMENT                              "NheI: 2 sites") 1 5 "GCTAGC" "CGATCG") 
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COMMENT                              "NruI: 1 site") 3 3 "TCGCGA" "AGCGCT") 
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COMMENT                                      "Georgia") 1 0.555556 0 
COMMENT                                     "{\\i Bst}YI (2982)" "@N (@S)" 5 0 0 0 -10 
COMMENT                                     -1 -1 #1826#) 
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FEATURES             Location/Qualifiers
     misc_feature    3828..4685
                     /vntifkey="21"
                     /label=AmpR
                     /note="AmpR"
     misc_feature    1..167
                     /vntifkey="21"
                     /label=5'\ITR
                     /note="5' ITR"
     misc_feature    3003..3169
                     /vntifkey="21"
                     /label=3'\ITR
                     /note="3' ITR"
     misc_feature    696..2108
                     /vntifkey="21"
                     /label=NES-jRGECO1b
                     /note="NES-jRGECO1b"
     polyA_signal    2743..2974
                     /vntifkey="25"
                     /label=SV40\polyadenylation\signal
                     /note="SV40 polyadenylation signal"
     promoter        190..661
                     /vntifkey="29"
                     /label=synapsin\promoter
                     /note="synapsin promoter"
     misc_feature    2121..2709
                     /vntifkey="21"
                     /label=WPRE
                     /note="WPRE"
BASE COUNT     1313 a      1514 c      1553 g      1304 t 
ORIGIN
        1 tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc cgggcgtcgg gcgacctttg 
       61 gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta 
      121 ggggttcctt gtagttaatg attaacccgc catgctactt atctacgtag ccatgctcta 
      181 ggaagatctc tgcagagggc cctgcgtatg agtgcaagtg ggttttagga ccaggatgag 
      241 gcggggtggg ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc 
      301 cattccccaa attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg 
      361 cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc 
      421 gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac 
      481 tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca 
      541 cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc 
      601 agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc 
      661 gaattcaagc tgctagcaag gatccacccg ccaccatgct gcagaacgag cttgctctta 
      721 agttggctgg acttgatatt aacaagactg gaggaggttc tcatcatcat catcatcatg 
      781 gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata 
      841 aggatctcgc aacaatggtc gactcatcac gtcgtaagtg gaataaggca ggtcacgcag 
      901 tcagagctat aggtcggctg agctcacccg tggtttccga gcggatgtac cccgaggacg 
      961 gcgcccttaa gagcgagatc aagaaggggc tgaggctgaa ggacggcggc cactacgccg 
     1021 ccgaggtcaa gactacctac aaggccaaga agcccgtgca gctgcccggc gcctacaagg 
     1081 tggacatcaa gttggacatc gtctcccaca acgaggacta caccatcgtg gaacagtgcg 
     1141 aatgtgccga gggccgccac tccaccggcg gcatggacga gctgtacaag ggaggtacag 
     1201 gcgggagtct ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc 
     1261 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg 
     1321 gcgagggccg cccctacgag gcctttcaga ccgctaagct gaaggtgacc aagggtggcc 
     1381 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctaca 
     1441 ttaagcaccc agccgacatc cccgactact tcaagctgtc cttccccgag ggcttcaggt 
     1501 gggagcgcgt gatgaacttc gaggacggcg gcattattca cgtgaaccag gactcctccc 
     1561 tgcaggacgg cgtattcatc tacaaggtga agctgcgcgg caccaacttc cctccggacg 
     1621 gccccgtaat gcagaagaag accatgggct gggaggctac gcgtgaccaa ctgactgaag 
     1681 agcagatcgc agaatttaaa gaggctttct cctgctttga caaggacggg gatgggacga 
     1741 taacaaccaa ggagcttggg acggtgatgc gatcgctggg gcagaacccc acagaagcag 
     1801 agctgcagga catgatcaat gaagtagatg ccgacggtga cggcacattc gacttccctg 
     1861 agttcctgac gatgatggca agaaaaatga atgacacaga cagtgaagag gaaattcgcg 
     1921 aagcgttccg cgtgtttgat aaggacggca atggctacat cggcgcagca gagcttcgcc 
     1981 acgtgatgac agaccttgga gagaagttaa cagatgagga ggttgatgaa atgatcaggg 
     2041 tagcagacat cgatggggat ggtcaggtaa actacgaaga gtttgtacaa atgatgacag 
     2101 cgaagtgaaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg 
     2161 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt 
     2221 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc 
     2281 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt 
     2341 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga 
     2401 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct 
     2461 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat 
     2521 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct 
     2581 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc 
     2641 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg 
     2701 cctccccgca tcgataccgt cgacctcgac ccgggcggcc gcttcgagca gacatgataa 
     2761 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt 
     2821 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta 
     2881 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt 
     2941 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatcttcct agagcatggc 
     3001 tacgtagata agtagcatgg cgggttaatc attaactaca aggaacccct agtgatggag 
     3061 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc 
     3121 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctggcgtaat 
     3181 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg 
     3241 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 
     3301 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 
     3361 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 
     3421 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg 
     3481 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 
     3541 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 
     3601 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 
     3661 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg 
     3721 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 
     3781 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 
     3841 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 
     3901 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 
     3961 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 
     4021 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 
     4081 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 
     4141 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 
     4201 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 
     4261 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 
     4321 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 
     4381 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 
     4441 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 
     4501 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 
     4561 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 
     4621 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 
     4681 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 
     4741 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 
     4801 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 
     4861 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 
     4921 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 
     4981 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca 
     5041 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 
     5101 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 
     5161 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 
     5221 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 
     5281 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 
     5341 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 
     5401 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 
     5461 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 
     5521 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 
     5581 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac 
     5641 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagc 
//
