LOCUS       pAAV.GfaABC1D.GluSnFr.SV40\(p4618)_NGS        6291 bp    DNA     circular      5-JUL-2017
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11837828|
COMMENT     VNTDBDATE|-11837828|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV.GfaABC1D.GluSnFr.SV40 (p4618)_NGS|
COMMENT     VNTAUTHORNAME|Matthew Chrzanowski|
COMMENT     VNTOAUTHORNAME|Bal Khakh|
COMMENT     VNTOAUTHORTEL|310-825-7185|
COMMENT     VNTOAUTHOREML|bkhakh@mednet.ucla.ediu|
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FEATURES             Location/Qualifiers
     LTR             1..168
                     /vntifkey="19"
                     /label=5'ITR
     promoter        191..871
                     /vntifkey="29"
                     /label=GfaABC1D
     intron          878..1187
                     /vntifkey="15"
                     /label=Promega\Chimeric\Intron
     LTR             complement(3303..3470)
                     /vntifkey="19"
                     /label=3'ITR
     polyA_signal    3043..3275
                     /vntifkey="25"
                     /label=SV40
     CDS             1211..3034
                     /vntifkey="4"
                     /label=GluSnFr
     CDS             4233..5090
                     /vntifkey="4"
                     /label=Amp-R
     mutation        2061..2061
                     /vntifkey="62"
                     /label=A\to\G;\Lys\to\Arg
     mutation        2422..2422
                     /vntifkey="62"
                     /label=C\to\T;\Silent\mutation\Gly
BASE COUNT     1559 a      1657 c      1641 g      1434 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagatct aacatatcct ggtgtggagt aggggacgct gctctgacag aggctcgggg 
      241 gcctgagctg gctctgtgag ctggggagga ggcagacagc caggccttgt ctgcaagcag 
      301 acctggcagc attgggctgg ccgcccccca gggcctcctc ttcatgccca gtgaatgact 
      361 caccttggca cagacacaat gttcggggtg ggcacagtgc ctgcttcccg ccgcacccca 
      421 gcccccctca aatgccttcc gagaagccca ttgagcaggg ggcttgcatt gcaccccagc 
      481 ctgacagcct ggcatcttgg gataaaagca gcacagcccc ctaggggctg cccttgctgt 
      541 gtggcgccac cggcggtgga gaacaaggct ctattcagcc tgtgcccagg aaaggggatc 
      601 aggggatgcc caggcatgga cagtgggtgg caggggggga gaggagggct gtctgcttcc 
      661 cagaagtcca aggacacaaa tgggtgaggg gagagctctc cccatagctg ggctgcggcc 
      721 caaccccacc ccctcaggct atgccagggg gtgttgccag gggcacccgg gcatcgccag 
      781 tctagcccac tccttcataa agccctcgca tcccaggagc gagcagagcc agagcaggtt 
      841 ggagaggaga cgcatcacct ccgctgctcg caagctttat tgcggtagtt tatcacagtt 
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt 
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat 
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc 
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct 
     1141 cttaaggcta gagtacttaa tacgactcac tataggctag cctcgagaat tcggcttggg 
     1201 gatatccacc atggagacag acacactcct gctatgggta ctgctgctct gggttccagg 
     1261 ttccactggt gacagatctg ccgcaggcag cacgctggac aaaatcgcca aaaacggtgt 
     1321 gattgtcgtc ggtcaccgtg aatcttcagt gcctttctct tattacgaca atcagcaaaa 
     1381 agtggtgggt tactcgcagg attactccaa cgccattgtt gaagcagtga aaaagaaact 
     1441 caacaaaccg gacttgcagg taaaactgat tccgattacc tcacaaaacc gtattccact 
     1501 gctgcaaaac ggcactttcg attttgaatg tggttctacc accaacaacg tcgaacgcca 
     1561 aaaacaggcg gctttctctg acactatttt cgtggtcggt acgcgcctgt tgaccaaaaa 
     1621 gggtggcgat atcaaagatt ttgccaacct gaaagacaaa gccgtagtcg tcacttccgg 
     1681 cactacctct gaagttttgc tcaacaaact gaatgaagag caaaaaatga atatgcgcat 
     1741 catcagcgcc aaagatcacg gtgactcttt ccgcaccctg gaaagcggtc gtgccgttgc 
     1801 ctttatgatg gatgacgtgc tgctggccgg tgaacgtgcg aaagcgaaga aaccagacaa 
     1861 ctgggaaatc gtcggcaagc cgcagtctca ggaggcctac ggttgtatgt tgcgtaaaga 
     1921 tgatccgcag ttcaaaaagc tgatggatga caccatcgct caggtgcaga cctccggtga 
     1981 agcggaaaaa tggtttgata agtggttcaa aaatccaatt ctggtgagcc acaacgtcta 
     2041 tatcatggcc gacaagcaga ggaacggcat caaggcgaac ttcaagatcc gccacaacat 
     2101 cgaggacggc ggcgtgcagc tcgcctacca ctaccagcag aacaccccca tcggcgacgg 
     2161 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaaacttt cgaaagaccc 
     2221 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct 
     2281 cggcatggac gagctgtaca agggcggtac cggagggagc atggtgagca agggcgagga 
     2341 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa 
     2401 gttcagcgtg tccggcgagg gtgagggcga tgccacctac ggcaagctga ccctgaagtt 
     2461 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta 
     2521 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc 
     2581 cgccatgccc gaaggctaca tccaggagcg caccatcttc ttcaaggacg acggcaacta 
     2641 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa 
     2701 gggcatcgac ttcaaggagg acggcaacat cctggggcat aagctggagt acaactttaa 
     2761 caaccccctg aacatgaatt tcgaactgtc agacgaaatg aaagcactgt tcaaagaacc 
     2821 gaatgacaag gcactgaaac tgcaggtcga cgaacaaaaa ctcatctcag aagaggatct 
     2881 gaatgctgtg ggccaggaca cgcaggaggt catcgtggtg ccacactcct tgccctttaa 
     2941 ggtggtggtg atctcagcca tcctggccct ggtggtgctc accatcatct cccttatcat 
     3001 cctcatcatg ctttggcaga agaagccacg ttaggcggcc gcttcgagca gacatgataa 
     3061 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt 
     3121 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta 
     3181 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt 
     3241 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatcttcct agagcatggc 
     3301 tacgtagata agtagcatgg cgggttaatc attaactaca aggaacccct agtgatggag 
     3361 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc 
     3421 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ccttaattaa 
     3481 cctaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 
     3541 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc 
     3601 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc 
     3661 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 
     3721 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 
     3781 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 
     3841 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 
     3901 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 
     3961 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg 
     4021 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac 
     4081 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta 
     4141 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 
     4201 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 
     4261 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 
     4321 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 
     4381 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 
     4441 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 
     4501 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 
     4561 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 
     4621 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 
     4681 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 
     4741 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 
     4801 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 
     4861 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 
     4921 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 
     4981 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 
     5041 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 
     5101 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 
     5161 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 
     5221 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 
     5281 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 
     5341 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 
     5401 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 
     5461 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 
     5521 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 
     5581 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 
     5641 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 
     5701 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 
     5761 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 
     5821 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 
     5881 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 
     5941 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 
     6001 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 
     6061 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 
     6121 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 
     6181 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag 
     6241 gaaacagcta tgaccatgat tacgccagat ttaattaagg ccttaattag g 
//
