LOCUS       Exported                6082 bp ds-DNA     circular SYN 19-SEP-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 19, 2017 from SnapGene 4.0.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6082
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=pCR3.1 with native PRKAKA isoform 1"
     misc_feature    11..627
                     /label=CMV_immearly_promoter
                     /note="CMV_immearly_promoter"
     misc_feature    544..564
                     /label=CMV F primer
                     /note="CMV F primer"
     misc_feature    638..656
                     /label=T7 promoter
                     /note="T7 promoter"
     misc_feature    704..709
                     /label=Added BamHi site for beginning
                     /note="Added BamHi site for beginning"
     misc_feature    710..718
                     /label=Added for consensus kozak
                     /note="Added for consensus kozak"
     misc_feature    719..764
                     /label=Exon 1 from PRKACA protein_id=%22NP_002721.1%22
                     /note="Exon 1 from PRKACA protein_id=%22NP_002721.1%22"
     misc_feature    765..826
                     /note="PRKAKA Exon 2 from protein kinase, cAMP-dependent, 
                     catalytic, alpha, transcript variant 1 
                     transcript_id=%22NM_002730.3%22"
     misc_feature    827..955
                     /note="PRKAKA Exon 3 from protein kinase, cAMP-dependent, 
                     catalytic, alpha, transcript variant 1 
                     transcript_id=%22NM_002730.3%22"
     misc_feature    956..1054
                     /note="PRKAKA Exon 4 from protein kinase, cAMP-dependent, 
                     catalytic, alpha, transcript variant 1 
                     transcript_id=%22NM_002730.3%22"
     misc_feature    1055..1137
                     /note="PRKAKA Exon 5 from protein kinase, cAMP-dependent, 
                     catalytic, alpha, transcript variant 1 
                     transcript_id=%22NM_002730.3%22"
     misc_feature    1138..1264
                     /note="PRKAKA Exon 6 from protein kinase, cAMP-dependent, 
                     catalytic, alpha, transcript variant 1 
                     transcript_id=%22NM_002730.3%22"
     misc_feature    1265..1360
                     /note="PRKAKA Exon 7 from protein kinase, cAMP-dependent, 
                     catalytic, alpha, transcript variant 1 
                     transcript_id=%22NM_002730.3%22"
     misc_feature    1361..1483
                     /note="PRKAKA Exon 8 from protein kinase, cAMP-dependent, 
                     catalytic, alpha, transcript variant 1 
                     transcript_id=%22NM_002730.3%22"
     misc_feature    1484..1648
                     /note="PRKAKA Exon 9 from protein kinase, cAMP-dependent, 
                     catalytic, alpha, transcript variant 1 
                     transcript_id=%22NM_002730.3%22"
     misc_feature    1649..1774
                     /note="PRKAKA Exon 10 from protein kinase,
                     cAMP-dependent, catalytic, alpha, transcript variant 1 
                     transcript_id=%22NM_002730.3%22"
     misc_feature    1775..1784
                     /label=Added extra stop codons
                     /note="Added extra stop codons"
     misc_feature    1829..1846
                     /label=BGH reverse primer
                     /note="BGH reverse primer"
     misc_feature    1832..2059
                     /label=bGH Poly Adenylation sequence terminator
                     /note="bGH Poly Adenylation sequence terminator"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagctgg ctagcgttta aacttaagct tggtaccgag ctcggatccg ccgccaccat
      721 gggcaacgcc gccgccgcca agaagggcag cgagcaggag agcgtgaaag aattcttagc
      781 caaagccaaa gaagattttc ttaaaaaatg ggaaagtccc gctcagaaca cagcccactt
      841 ggatcagttt gaacgaatca agaccctcgg cacgggctcc ttcgggcggg tgatgctggt
      901 gaaacacaag gagaccggga accactatgc catgaagatc ctcgacaaac agaaggtggt
      961 gaaactgaaa cagatcgaac acaccctgaa tgaaaagcgc atcctgcaag ctgtcaactt
     1021 tccgttcctc gtcaaactcg agttctcctt caaggacaac tcaaacttat acatggtcat
     1081 ggagtacgtg cccggcgggg agatgttctc acacctacgg cggatcggaa ggttcagtga
     1141 gccccatgcc cgtttctacg cggcccagat cgtcctgacc tttgagtatc tgcactcgct
     1201 ggatctcatc tacagggacc tgaagccgga gaatctgctc attgaccagc agggctacat
     1261 tcaggtgaca gacttcggtt tcgccaagcg cgtgaagggc cgcacttgga ccttgtgcgg
     1321 cacccctgag tacctggccc ctgagattat cctgagcaaa ggctacaaca aggccgtgga
     1381 ctggtgggcc ctgggggttc ttatctatga aatggccgct ggctacccgc ccttcttcgc
     1441 agaccagccc atccagatct atgagaagat cgtctctggg aaggtgcgct tcccttccca
     1501 cttcagctct gacttgaagg acctgctgcg gaacctcctg caggtagatc tcaccaagcg
     1561 ctttgggaac ctcaagaatg gggtcaacga tatcaagaac cacaagtggt ttgccacaac
     1621 tgactggatt gccatctacc agaggaaggt ggaagctccc ttcataccaa agtttaaagg
     1681 ccctggggat acgagtaact ttgacgacta tgaggaagaa gaaatccggg tctccatcaa
     1741 tgagaagtgt ggcaaggagt tttctgagtt ttagtaatga ttaagcggcc gctcgagtct
     1801 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     1861 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     1921 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     1981 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     2041 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gtggcggtaa tacggttatc
     2101 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     2161 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2221 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2281 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2341 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2401 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2461 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2521 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2581 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2641 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2701 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2761 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2821 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     2881 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaacctga
     2941 ggctatggca gggcctgccg ccccgacgtt ggctgcgagc cctgggcctt cacccgaact
     3001 tggggggtgg ggtggggaaa aggaagaaac gcgggcgtat tggccccaat ggggtctcgg
     3061 tggggtatcg acagagtgcc agccctggga ccgaaccccg cgtttatgaa caaacgaccc
     3121 aacaccgtgc gttttattct gtctttttat tgccgtcata gcgcgggttc cttccggtat
     3181 tgtctccttc cgtgtttcag ttagcctccc cctagggtgg gcgaagaact ccagcatgag
     3241 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
     3301 ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc
     3361 ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg
     3421 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     3481 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc
     3541 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     3601 ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg
     3661 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     3721 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     3781 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     3841 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     3901 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     3961 cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg
     4021 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     4081 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     4141 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct
     4201 gtctcttgat cgatctttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     4261 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     4321 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     4381 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     4441 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
     4501 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt
     4561 ctttccgcct caggactctt cctttttcaa taaatcaatc taaagtatat atgagtaaac
     4621 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     4681 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     4741 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     4801 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     4861 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     4921 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     4981 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     5041 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     5101 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     5161 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     5221 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     5281 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     5341 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     5401 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     5461 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     5521 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     5581 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg
     5641 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     5701 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     5761 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     5821 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     5881 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5941 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     6001 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     6061 aatattaacg cttacaattt ac
//
