LOCUS       Exported                8151 bp ds-DNA     circular SYN 07-SEP-2017
DEFINITION  Ligation of NoName* into  SGL40C-NL-SL.EFS.dTomato*.
ACCESSION   .
VERSION     .
KEYWORDS    SGL40C-NL-SL.EFS.E2Crimson
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 7, 2017 from SnapGene Viewer 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8151
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    430..811
                     /label=CMV IE enhancer
     misc_feature    826..1058
                     /label=SV40 enhancer
     misc_feature    1075..1487
                     /label=RSV/5'LTR
                     /note="LTR"
     misc_feature    1538..1675
                     /label=psi
     misc_feature    1642..1996
                     /label=gag'
     misc_feature    2911..3028
                     /label=cPPT
     misc_feature    3079..3327
                     /label=U6 Prom
     misc_feature    3355..3446
                     /label=sgRNA
     misc_feature    3359
                     /label=mod T-A
     misc_feature    3367..3371
                     /label=elong stem
     misc_feature    3376..3380
                     /label=elong stem comp
     misc_feature    3390
                     /label=mod A-T
     misc_feature    3469..3713
                     /label=EFS
     misc_feature    3726..4403
                     /label=E2Crimson
     misc_feature    4419..5007
                     /label=WPRE
                     /note="misc_RNA"
     misc_feature    5078..5313
                     /label=3'\LTR\SIN
                     /note="LTR"
     misc_feature    5390..5520
                     /label=SV40\polyA
                     /note="polyA_signal"
     misc_feature    5867..6297
                     /label=f1\Ori
                     /note="rep_origin"
     misc_feature    6668..7474
                     /label=AmpR
     misc_feature    complement(7602..64)
                     /label=pUC\Ori
                     /note="rep_origin"
ORIGIN
        1 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
       61 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      121 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      181 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      241 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      301 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      361 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cggtaccgtc
      421 gacctgcagg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      481 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      541 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      601 cgccaatagg gactttccat tgacgtcaat gggtggacta tttacggtaa actgcccact
      661 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      721 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      781 acatctacgt attagtcatc gctattacca tggcgcgcca tgcagggcct gaaataacct
      841 ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt
      901 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
      961 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     1021 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc ttaagaggcg cgccttaatg
     1081 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     1141 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     1201 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     1261 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     1321 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     1381 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     1441 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     1501 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     1561 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     1621 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     1681 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     1741 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     1801 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     1861 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     1921 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     1981 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     2041 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     2101 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     2161 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     2221 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     2281 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     2341 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     2401 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     2461 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     2521 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     2581 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     2641 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     2701 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     2761 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     2821 gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt
     2881 ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     2941 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     3001 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     3061 ttggttaatt aaggtaccga gggcctattt cccatgattc cttcatattt gcatatacga
     3121 tacaaggctg ttagagagat aattagaatt aatttgactg taaacacaaa gatattagta
     3181 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
     3241 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
     3301 tatatcttgt ggaaaggacg aaacaccgga gacgtatgtc gacaactcgt ctctgtttaa
     3361 gagctatgct ggaaacagca tagcaagttt aaataaggct agtccgttat caacttgaaa
     3421 aagtggcacc gagtcggtgc ttttttgaat tctcctctag aactcgagtg gctccggtgc
     3481 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     3541 caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     3601 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     3661 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg
     3721 ccaccatgga tagcactgag aacgtcatca agcccttcat gcgcttcaag gtgcacatgg
     3781 agggctccgt gaacggccac gagttcgaga tcgagggcgt gggcgagggc aagccctacg
     3841 agggcaccca gaccgccaag ctgcaagtga ccaagggcgg ccccctgccc ttcgcctggg
     3901 acatcctgtc cccccagttc ttctacggct ccaaggcgta catcaagcac cccgccgaca
     3961 tccccgacta cctcaagcag tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     4021 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcaccctca
     4081 tctaccacgt gaagttcatc ggcgtgaact tcccctccga cggccccgta atgcagaaga
     4141 agactctggg ctgggagccc tccactgagc gcaactaccc ccgcgacggc gtgctgaagg
     4201 gcgagaacca catggcgctg aagctgaagg gcggcggcca ctacctgtgt gagttcaagt
     4261 ccatctacat ggccaagaag cccgtgaagc tgcccggcta ccactacgtg gactacaagc
     4321 tcgacatcac ctcccacaac gaggactaca ccgtggtgga gcagtacgag cgcgccgagg
     4381 cccgccacca cctgttccag tagacgcgtt aagtcgacaa tcaacctctg gattacaaaa
     4441 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     4501 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     4561 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     4621 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     4681 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     4741 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     4801 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     4861 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     4921 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     4981 ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc
     5041 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     5101 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     5161 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     5221 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     5281 acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat
     5341 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     5401 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5461 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     5521 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5581 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     5641 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     5701 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     5761 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     5821 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     5881 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     5941 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     6001 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     6061 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     6121 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     6181 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     6241 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     6301 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     6361 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     6421 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     6481 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     6541 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     6601 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6661 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6721 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6781 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6841 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6901 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6961 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     7021 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7081 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     7141 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     7201 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7261 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     7321 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     7381 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7441 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7501 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7561 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7621 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     7681 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7741 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7801 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7861 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7921 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7981 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8041 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8101 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg c
//
