LOCUS       pAAV.GFA104.PI.eGFP.WPRE.bGH\(p2380-Q)        5673 bp    DNA     circular     19-JUN-2013
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|665243011|
COMMENT     VNTDBDATE|665243011|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV.GFA104.PI.eGFP.WPRE.bGH (p2380-Q)|
COMMENT     VNTAUTHORNAME|Shu-Jen Chen|
COMMENT     VNTOAUTHORNAME|Ruben Hovhannisyan|
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FEATURES             Location/Qualifiers
     misc_feature    230..910
                     /vntifkey="21"
                     /label=GFA104\promoter
     CDS             3626..4486
                     /vntifkey="4"
                     /label=Amp-R
     CDS             1150..1869
                     /vntifkey="4"
                     /label=EGFP
     LTR             1..130
                     /vntifkey="19"
                     /label=5'\ITR
     LTR             complement(2733..2862)
                     /vntifkey="19"
                     /label=3'\ITR
     misc_feature    4657..5245
                     /vntifkey="21"
                     /label=pBR322\origin
     misc_feature    complement(3039..3494)
                     /vntifkey="21"
                     /label=f1\origin
     misc_marker     1885..2424
                     /vntifkey="22"
                     /label=WPRE
     insertion_seq   2431..2645
                     /vntifkey="14"
                     /label=bGH\polyA
     misc_feature    952..952
                     /vntifkey="21"
                     /label=C->T
     intron          1000..1132
                     /vntifkey="15"
                     /label=PI
BASE COUNT     1263 a      1568 c      1525 g      1317 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagatcg gaattcgccc ttaagctagc cgagctcggt accagatcta acatatcctg 
      241 gtgtggagta ggggacgctg ctctgacaga ggctcggggg cctgagctgg ctctgtgagc 
      301 tggggaggag gcagacagcc aggccttgtc tgcaagcaga cctggcagca ttgggctggc 
      361 cgccccccag ggcctcctct tcatgcccag tgaatgactc accttggcac agacacaatg 
      421 ttcggggtgg gcacagtgcc tgcttcccgc cgcaccccag cccccctcaa atgccttccg 
      481 agaagcccat tgagcagggg gcttgcattg caccccagcc tgacagcctg gcatcttggg 
      541 ataaaagcag cacagccccc taggggctgc ccttgctgtg tggcgccacc ggcggtggag 
      601 aacaaggctc tattcagcct gtgcccagga aaggggatca ggggatgccc aggcatggac 
      661 agtgggtggc agggggggag aggagggctg tctgcttccc agaagtccaa ggacacaaat 
      721 gggtgagggg agagctctcc ccatagctgg gctgcggccc aaccccaccc cctcaggcta 
      781 tgccaggggg tgttgccagg ggcacccggg catcgccagt ctagcccact ccttcataaa 
      841 gccctcgcat cccaggagcg agcagagcca gagcaggttg gagaggagac gcatcacctc 
      901 cgctgctcgc ggggatcctc tagagtcgac ggatccgggg aattccccag tttcaggatc 
      961 caccatggct gcagaagttg gtcgtgaggc actgggcagg taagtatcaa ggttacaaga 
     1021 caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact cttgcgtttc 
     1081 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtgtccag 
     1141 gcggccgcca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 
     1201 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 
     1261 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc 
     1321 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac 
     1381 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc 
     1441 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 
     1501 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 
     1561 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag 
     1621 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg 
     1681 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc 
     1741 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat 
     1801 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg 
     1861 tacaagtaat aagcttggat ccaatcaacc tctggattac aaaatttgtg aaagattgac 
     1921 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt 
     1981 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt 
     2041 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt 
     2101 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg 
     2161 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg 
     2221 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc 
     2281 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt 
     2341 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc 
     2401 tctgcggcct cttccgcgtc ttcgagatct gcctcgactg tgccttctag ttgccagcca 
     2461 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc 
     2521 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg 
     2581 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct 
     2641 ggggactcga gttaagggcg aattcccgat aaggatcttc ctagagcatg gctacgtaga 
     2701 taagtagcat ggcgggttaa tcattaacta caaggaaccc ctagtgatgg agttggccac 
     2761 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc 
     2821 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agccttaatt aacctaattc 
     2881 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg 
     2941 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg 
     3001 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt 
     3061 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 
     3121 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 
     3181 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 
     3241 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccccgat agacggtttt 
     3301 tcgccctttg acgctggagt tcacgttcct caatagtgga ctcttgttcc aaactggaac 
     3361 aacactcaac cctatctcgg tctattcttt tgatttataa gggatttttc cgatttcggc 
     3421 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt 
     3481 aacgtttata atttcaggtg gcatctttcg gggaaatgtg cgcggaaccc ctatttgttt 
     3541 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 
     3601 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc 
     3661 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa 
     3721 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaatagtgg 
     3781 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt 
     3841 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg 
     3901 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac 
     3961 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc 
     4021 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa 
     4081 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc 
     4141 aaacgacgag cgtgacacca cgatgcctgt agtaatggta acaacgttgc gcaaactatt 
     4201 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga 
     4261 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 
     4321 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa 
     4381 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa 
     4441 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 
     4501 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 
     4561 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 
     4621 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 
     4681 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 
     4741 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 
     4801 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 
     4861 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 
     4921 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 
     4981 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 
     5041 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 
     5101 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 
     5161 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 
     5221 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 
     5281 cttttgctgc ggttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 
     5341 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 
     5401 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg 
     5461 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga 
     5521 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat 
     5581 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag 
     5641 ctatgaccat gattacgcca gatttaatta agg 
//
