LOCUS       1377_stringent_K        3022 bp ds-DNA     linear       30-AUG-2017
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1001..2022
                     /organism="Cloning vector pYT923hyg"
                     /mol_type="other DNA"
                     /db_xref="taxon:661031"
                     /note="Geneious name: source Cloning vector pYT923hyg"
                     /label=Geneious name: source Cloning vector pYT923hyg
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            complement(1001..2022)
                     /created_by="User"
                     /note="Geneious name: KanR from AB513495"
                     /label=Geneious name: KanR from AB513495
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            complement(1001..2022)
                     /created_by="User"
                     /note="Geneious name: KanR cassette"
                     /label=Geneious name: KanR cassette
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(1034..1849)
                     /function="kanamaycin resistance"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BAH86059.1"
                     /db_xref="GI:254674504"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
                     /modified_by="User"
                     /note="Geneious name: KanR"
                     /label=hypothetical protein
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(2022..2022)
                     /created_by="User"
                     /modified_by="User"
                     /note="Geneious name: RpaA genomic DNA -start of gene"
                     /label=Geneious name: RpaA genomic DNA -start of gene
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1910..1936
                     /created_by="User"
                     /note="Geneious name: JM064"
                     /label=Geneious name: JM064
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1001..1025
                     /created_by="User"
                     /note="Geneious name: JM065"
                     /label=Geneious name: JM065
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1001..1025
                     /created_by="User"
                     /note="Geneious name: JM079"
                     /label=Geneious name: JM079
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1914..1936
                     /created_by="User"
                     /note="Geneious name: JM043"
                     /label=Geneious name: JM043
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(1129..1148)
                     /created_by="User"
                     /note="Geneious name: JM042"
                     /label=Geneious name: JM042
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(2004..2021)
                     /created_by="User"
                     /modified_by="User"
                     /note="Geneious name: JM080"
                     /label=Geneious name: JM080
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(2004..2020)
                     /created_by="User"
                     /modified_by="User"
                     /note="Geneious name: JM066"
                     /label=Geneious name: JM066
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1723..1728
                     /Recognition_pattern="AT^CGAT"
                     /annotation_group="ATCGAT"
                     /note="Geneious name: ClaI"
                     /note="Geneious type: restriction site"
                     /label=Geneious name: ClaI
                     /ApEinfo_fwdcolor=#e6ff19
                     /ApEinfo_revcolor=#ff1527
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1758..1763
                     /Recognition_pattern="TCG^CGA"
                     /annotation_group="TCGCGA"
                     /note="Geneious name: NruI"
                     /note="Geneious type: restriction site"
                     /label=Geneious name: NruI
                     /ApEinfo_fwdcolor=#e6ff19
                     /ApEinfo_revcolor=#ff1527
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1416..1421
                     /Recognition_pattern="CGAT^CG"
                     /annotation_group="CGATCG"
                     /note="Geneious name: PvuI"
                     /note="Geneious type: restriction site"
                     /label=Geneious name: PvuI
                     /ApEinfo_fwdcolor=#e6ff19
                     /ApEinfo_revcolor=#ff1527
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1541..1546
                     /Recognition_pattern="C^CCGGG"
                     /annotation_group="CCCGGG"
                     /note="Geneious name: XmaI"
                     /note="Geneious type: restriction site"
                     /label=Geneious name: XmaI
                     /ApEinfo_fwdcolor=#e6ff19
                     /ApEinfo_revcolor=#ff1527
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1541..1546
                     /Recognition_pattern="CCC^GGG"
                     /annotation_group="CCCGGG"
                     /note="Geneious name: SmaI"
                     /note="Geneious type: restriction site"
                     /label=Geneious name: SmaI
                     /ApEinfo_fwdcolor=#e6ff19
                     /ApEinfo_revcolor=#ff1527
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1815..1820
                     /Recognition_pattern="C^TCGAG"
                     /annotation_group="CTCGAG"
                     /note="Geneious name: XhoI"
                     /note="Geneious type: restriction site"
                     /label=Geneious name: XhoI
                     /ApEinfo_fwdcolor=#e6ff19
                     /ApEinfo_revcolor=#ff1527
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1295..1300
                     /Recognition_pattern="A^AGCTT"
                     /annotation_group="AAGCTT"
                     /note="Geneious name: HindIII"
                     /note="Geneious type: restriction site"
                     /label=Geneious name: HindIII
                     /ApEinfo_fwdcolor=#e6ff19
                     /ApEinfo_revcolor=#ff1527
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1581..1584
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /note="Geneious name: RsaI"
                     /note="Geneious type: restriction site"
                     /label=Geneious name: RsaI
                     /ApEinfo_fwdcolor=#e6ff19
                     /ApEinfo_revcolor=#ff1527
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1296..1299
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /note="Geneious name: AluI"
                     /note="Geneious type: restriction site"
                     /label=Geneious name: AluI
                     /ApEinfo_fwdcolor=#e6ff19
                     /ApEinfo_revcolor=#ff1527
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1001..1959
                     /label=New Feature
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1001..2022
                     /label=New Feature(1)
                     /ApEinfo_label=New Feature
                     /ApEinfo_fwdcolor=#fff240
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 TGGATGGCGG CATAACTGGC GCTAGACATC GGGGCTAATC GACAGTGTCA AACCAAAACG
       61 GTTAGCCAGC CCTGAACAGC GATCGCTTCC AGCCAGACAG GGCAGCCGTT TCCACTTCTA
      121 GGCAGGTCAA CGTCCAGACT GCCGTTGATT CGAGCTTAAC GTTCCCTTAC CAAGCACTGG
      181 TCAGCAAGAC TTCCAGCACT TGATTGGGTT GAAGACTCGT CGCCGGCAAG CCACTCCCAA
      241 AGGCTGATGC AGCTCCGACA GCAGCCCCCC CGACTAACCC CACGGGTCCT CCAACCACTA
      301 GTCCTGCCGC TGCGCCTACA CCTGCATTGT TGGCAACACG ACCGCCATCA GCCGTAGGCG
      361 GGCCGGTGAT GACCTCTGAA ATGGCATAGA TCGGTACTGA GACTCCATTG AGGACAAGCG
      421 CTTGTGCAAC AAACCGACTG CCCTGCTGAT TAGTTTCAAA GCGGCCGAGC ACTGGCGTCC
      481 CGATCGGTAG GATGACCTGG CCCCTGCTAT CCGTGACATT TTGGTCAACA GTCATGACCT
      541 CTTGAATTGG TAGAGCGCTT TGCAAGCTCA GACCAGGACC TGTGTAGCGG AGCCGCACCA
      601 CAAACCGATC GCTCAAGGGT TTCAAGGGGG TTGTGCTGAG TGACGAGCCA CCGCCAGCCG
      661 GCACGGTCGG CAGTGGAATA CTGGACTGGG CCCAAGCGGT CGAAGCTGAC AGTGCGGCTA
      721 ACACGCCAGC GATCGCGACT GCAGATTTGC GCATCATTTC CATCCATCGC CTTTCGCACA
      781 GCTAGCTTGC CATTTTCCCA CCAGGAATCA AGACCTAGGT TTGCCGTTTG CACCAAGAAT
      841 GACTGGATTC AGACCCAGAA TCTTGCGATT CAGTCCACTA GCATTCCTGT CACCCCCCTG
      901 ACTCACAGGA AATTAACGGT TAAGATCGGT GCACAACTGC CGAGCTTCCC AATGAACTGC
      961 TGCAGTTGCA CCAGCCAAAA GGGGAGCGAT CGCACGTTCG ctgccagtgt tacaaccaat
     1021 taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
     1081 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
     1141 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     1201 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     1261 catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt
     1321 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     1381 tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac
     1441 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
     1501 ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga
     1561 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     1621 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     1681 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     1741 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     1801 aatttaatcg cggcctcgag caagacgttt cccgttgaat atggctcata acaccccttg
     1861 tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg
     1921 caatgtaaca tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg
     1981 ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc agTCACTCAG CTATCCAACT
     2041 GATGCTGATA AATACCGCCT ACAGGCACTT ATTGCGCTGC AGGAGTTAGC AATTGAGCCG
     2101 CGATCGCCGC TAGTTTTTGG GCCGCACCGG CCTCACCGCG AACCGCTCTG AGTTCCGCTT
     2161 GCATGGCCGA CAGTTTCTCT GGGTTCTGGA GATAGTCGAG AGCGATCGCG GCAATATCCT
     2221 CGGGATAGTA TTCGCCGATC AACTCGGGCA CGATCGCCCG CTTCGCCCAA ATATTCGGCC
     2281 AAGCCAGCAG ACCCACTTTG CGCAGCGCCA AGGTATTAAT CAGGCTAGCG ATCGCATTGC
     2341 CCAACAGTGG CACCTTGACC AGCAAGCCCG GCAAGCCGTC CCAGGCTTTC ATCGCATCCC
     2401 GTTTTTGGGT TGGCAGCAGC ACCAACATCG GCACCGCCAG CGCGCCTAAT TCTGCTGTAT
     2461 TCGCGCCGAT CGTGGTCAGG CAGATCTGGC ACTGGGCCAA CTCGACATAG GCTGGAAATT
     2521 CCGTGATCAA CCTTACGGTT AGCCCTTTTT CGGTCGTCAG CACCCAACCT GTGGGTTGCT
     2581 CTTCTAAACG GGCGGAACTA CCCCGAAACA TTGCAATGAC GGGATTTTGA TCCGCATCCG
     2641 CATAGCGAGC GATCTGCTGC GGTTGCACGG TCGGTGCGAG TGGCAGAATA AACTCCATCT
     2701 CTGGTTGCTG AGCCGCGATC GCTTCAGCCG CCGCCAGCAT GAAGGGCAAG CCGATCTGCA
     2761 ATTTAGCTGC TTTGGATCCG GGCAGAAGAC CCACCCGCGA TCGTGTGGAT TCAGATCCAG
     2821 CCTGCGCTGA GACCTCCGCC ATTAAATCGC CAACCAACTG AAACTTGTGA CGGTCTTTCT
     2881 GCGGTGCTTT CTCAATCACC TGGGGCGTCA TTACCGCAAA GGCATCAGCC CAGCCAGTCC
     2941 AGCGGGCTTC CCATTCCGCG TAGATCAAGC AGCGGTAGCC CAAGCGTCGG GCAATCCAGA
     3001 GGGCAAAAAA CTGATCGCCG CC   
//
