LOCUS       Exported               11127 bp ds-DNA     circular SYN 08-SEP-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 8, 2017 from SnapGene 4.0.4
            http://www.snapgene.com
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
            Cat. No.631191, 631193,631187, 631189
FEATURES             Location/Qualifiers
     source          1..11127
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        209..712
                     /label=pPGK
     misc_feature    713..720
                     /label=Unique cut site
     CDS             744..764
                     /codon_start=1
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    744..764
                     /label=Amino acid translation
     CDS             765..854
                     /codon_start=1
                     /label=3xHA
                     /translation="YPYDVPDYAAYPYDVPDYAAYPYDVPDYAA"
     misc_feature    855..860
                     /label=Amino acid translation
     misc_feature    861..5126
                     /label=Amino acid translation
     CDS             885..5000
                     /codon_start=1
                     /label=dCas9
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGDSRAD"
     variation       912..914
                     /label=D10A
     variation       3402..3404
                     /label=H840A
     CDS             5001..5126
                     /codon_start=1
                     /label=Linker V3
                     /translation="GGGSGGGSPKKKRKVTGKKTSGQAGQASPKKKRKVGRAGGGS"
     sig_peptide     5025..5045
                     /label=SV40 NLS
     sig_peptide     5085..5105
                     /label=SV40 NLS
     CDS             5127..6059
                     /codon_start=1
                     /label=DNMT3A
                     /translation="PSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGIATG
                     LLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKHIQEWGPFDLVI
                     GGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFENVVAMGVS
                     DKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLEHGR
                     IAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSR
                     LARQRLLGRSWSVPVIRHLFAPLKEYFACV"
     misc_feature    5127..6059
                     /label=Amino acid translation
     misc_feature    6060..6158
                     /label=New 3x Ty1
     sig_peptide     6159..6179
                     /label=SV40 NLS
     misc_feature    6180..6191
                     /label=P2A
     misc_feature    6195..6464
                     /label=HSV TK polyA
     promoter        6465..7722
                     /label=EF1a
     misc_feature    7729..7737
                     /label=Kozak sequence
     CDS             7735..8331
                     /codon_start=1
                     /label=Puro
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    8335..8632
                     /label=IVS
     polyA_signal    8640..8878
                     /label=SV40 polyA
     rep_origin      9357..9976
                     /label=pBR322 Orgin
     CDS             complement(10131..10991)
                     /codon_start=1
                     /label=Ampicillin
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11033..11061)
                     /label=AMP promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ttaattaata ccgggtaggg gaggcgcttt tcccaaggca
      241 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
      301 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
      361 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
      421 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
      481 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
      541 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
      601 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
      661 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cggcggccgc
      721 acctgcagcc caagcttacc atgccaaaga agaagcggaa ggtctacccc tatgacgtgc
      781 ctgattacgc cgcttatcct tacgatgtgc ccgactatgc agcctaccca tacgatgtgc
      841 ccgactacgc cgcaatggcc ggtatccacg gagtcccagc agccatggac aagaagtact
      901 ccattgggct cgctatcggc acaaacagcg tcggctgggc cgtcattacg gacgagtaca
      961 aggtgccgag caaaaaattc aaagttctgg gcaataccga tcgccacagc ataaagaaga
     1021 acctcattgg cgccctcctg ttcgactccg gggagacggc cgaagccacg cggctcaaaa
     1081 gaacagcacg gcgcagatat acccgcagaa agaatcggat ctgctacctg caggagatct
     1141 ttagtaatga gatggctaag gtggatgact ctttcttcca taggctggag gagtcctttt
     1201 tggtggagga ggataaaaag cacgagcgcc acccaatctt tggcaatatc gtggacgagg
     1261 tggcgtacca tgaaaagtac ccaaccatat atcatctgag gaagaagctt gtagacagta
     1321 ctgataaggc tgacttgcgg ttgatctatc tcgcgctggc gcatatgatc aaatttcggg
     1381 gacacttcct catcgagggg gacctgaacc cagacaacag cgatgtcgac aaactcttta
     1441 tccaactggt tcagacttac aatcagcttt tcgaagagaa cccgatcaac gcatccggag
     1501 ttgacgccaa agcaatcctg agcgctaggc tgtccaaatc ccggcggctc gaaaacctca
     1561 tcgcacagct ccctggggag aagaagaacg gcctgtttgg taatcttatc gccctgtcac
     1621 tcgggctgac ccccaacttt aaatctaact tcgacctggc cgaagatgcc aagcttcaac
     1681 tgagcaaaga cacctacgat gatgatctcg acaatctgct ggcccagatc ggcgaccagt
     1741 acgcagacct ttttttggcg gcaaagaacc tgtcagacgc cattctgctg agtgatattc
     1801 tgcgagtgaa cacggagatc accaaagctc cgctgagcgc tagtatgatc aagcgctatg
     1861 atgagcacca ccaagacttg actttgctga aggcccttgt cagacagcaa ctgcctgaga
     1921 agtacaagga aattttcttc gatcagtcta aaaatggcta cgccggatac attgacggcg
     1981 gagcaagcca ggaggaattt tacaaattta ttaagcccat cttggaaaaa atggacggca
     2041 ccgaggagct gctggtaaag cttaacagag aagatctgtt gcgcaaacag cgcactttcg
     2101 acaatggaag catcccccac cagattcacc tgggcgaact gcacgctatc ctcaggcggc
     2161 aagaggattt ctaccccttt ttgaaagata acagggaaaa gattgagaaa atcctcacat
     2221 ttcggatacc ctactatgtg ggccccctcg cccggggaaa ttccagattc gcgtggatga
     2281 ctcgcaaatc agaagagacc atcactccct ggaacttcga ggaagtcgtg gataaggggg
     2341 cctctgccca gtccttcatc gaaaggatga ctaactttga taaaaatctg cctaacgaaa
     2401 aggtgcttcc taaacactct ctgctgtacg agtacttcac agtttataac gagctcacca
     2461 aggtcaaata cgtcacagaa gggatgagaa agccagcatt cctgtctgga gagcagaaga
     2521 aagctatcgt ggacctcctc ttcaagacga accggaaagt taccgtgaaa cagctcaaag
     2581 aagactattt caaaaagatt gaatgtttcg actctgttga aatcagcgga gtggaggatc
     2641 gcttcaacgc atccctggga acgtatcacg atctcctgaa aatcattaaa gacaaggact
     2701 tcctggacaa tgaggagaac gaggacattc ttgaggacat tgtcctcacc cttacgttgt
     2761 ttgaagatag ggagatgatt gaagaacgct tgaaaactta cgctcatctc ttcgacgaca
     2821 aagtcatgaa acagctcaag aggcgccgat atacaggatg ggggcggctg tcaagaaaac
     2881 tgatcaatgg gatccgagac aagcagagtg gaaagacaat cctggatttt cttaagtccg
     2941 atggatttgc caacaggaac ttcatgcagt tgatccatga tgactctctc acctttaagg
     3001 aggacatcca gaaagcacaa gtttctggcc agggggacag tcttcacgag cacatcgcta
     3061 atcttgcagg tagcccagct atcaaaaagg gaatactgca gaccgttaag gtcgtggatg
     3121 aactcgtcaa agtaatggga aggcataagc ccgagaatat cgttatcgag atggcccgag
     3181 agaaccaaac tacccagaag ggacagaaga acagtaggga aaggatgaag aggattgaag
     3241 agggtataaa agaactgggg tcccaaatcc ttaaggaaca cccagttgaa aacacccagc
     3301 ttcagaatga gaagctctac ctgtactacc tgcagaacgg cagggacatg tacgtggatc
     3361 aggaactgga catcaatcgg ctctccgact acgacgtgga tgcaatcgtg ccccagtctt
     3421 ttctcaaaga tgattctatt gataataaag tgttgacaag atccgataaa aatagaggga
     3481 agagtgataa cgtcccctca gaagaagttg tcaagaaaat gaaaaattat tggcggcagc
     3541 tgctgaacgc caaactgatc acacaacgga agttcgataa tctgactaag gctgaacgag
     3601 gtggcctgtc tgagttggat aaagccggct tcatcaaaag gcagcttgtt gagacacgcc
     3661 agatcaccaa gcacgtggcc caaattctcg attcacgcat gaacaccaag tacgatgaaa
     3721 atgacaaact gattcgagag gtgaaagtta ttactctgaa gtctaagctg gtctcagatt
     3781 tcagaaagga ctttcagttt tataaggtga gagagatcaa caattaccac catgcgcatg
     3841 atgcctacct gaatgcagtg gtaggcactg cacttatcaa aaaatatccc aagcttgaat
     3901 ctgaatttgt ttacggagac tataaagtgt acgatgttag gaaaatgatc gcaaagtctg
     3961 agcaggaaat aggcaaggcc accgctaagt acttctttta cagcaatatt atgaattttt
     4021 tcaagaccga gattacactg gccaatggag agattcggaa gcgaccactt atcgaaacaa
     4081 acggagaaac aggagaaatc gtgtgggaca agggtaggga tttcgcgaca gtccggaagg
     4141 tcctgtccat gccgcaggtg aacatcgtta aaaagaccga agtacagacc ggaggcttct
     4201 ccaaggaaag tatcctcccg aaaaggaaca gcgacaagct gatcgcacgc aaaaaagatt
     4261 gggaccccaa gaaatacggc ggattcgatt ctcctacagt cgcttacagt gtactggttg
     4321 tggccaaagt ggagaaaggg aagtctaaaa aactcaaaag cgtcaaggaa ctgctgggca
     4381 tcacaatcat ggagcgatca agcttcgaaa aaaaccccat cgactttctc gaggcgaaag
     4441 gatataaaga ggtcaaaaaa gacctcatca ttaagcttcc caagtactct ctctttgagc
     4501 ttgaaaacgg ccggaaacga atgctcgcta gtgcgggcga gctgcagaaa ggtaacgagc
     4561 tggcactgcc ctctaaatac gttaatttct tgtatctggc cagccactat gaaaagctca
     4621 aagggtctcc cgaagataat gagcagaagc agctgttcgt ggaacaacac aaacactacc
     4681 ttgatgagat catcgagcaa ataagcgaat tctccaaaag agtgatcctc gccgacgcta
     4741 acctcgataa ggtgctttct gcttacaata agcacaggga taagcccatc agggagcagg
     4801 cagaaaacat tatccacttg tttactctga ccaacttggg cgcgcctgca gccttcaagt
     4861 acttcgacac caccatagac agaaagcggt acacctctac aaaggaggtc ctggacgcca
     4921 cactgattca tcagtcaatt acggggctct atgaaacaag aatcgacctc tctcagctcg
     4981 gtggagacag cagggctgac gggggaggat cagggggagg gtcccccaaa aagaaacgca
     5041 aagtgaccgg caagaaaacc agcggccagg ccggacaggc ctctcctaag aaaaaaagaa
     5101 aagtgggacg cgctggcggc ggaagcccct cccggctcca gatgttcttc gctaataacc
     5161 acgaccagga atttgaccct ccaaaggttt acccacctgt cccagctgag aagaggaagc
     5221 ccatccgggt gctgtctctc tttgatggaa tcgctacagg gctcctggtg ctgaaggact
     5281 tgggcattca ggtggaccgc tacattgcct cggaggtgtg tgaggactcc atcacggtgg
     5341 gcatggtgcg gcaccagggg aagatcatgt acgtcgggga cgtccgcagc gtcacacaga
     5401 agcatatcca ggagtggggc ccattcgatc tggtgattgg gggcagtccc tgcaatgacc
     5461 tctccatcgt caaccctgct cgcaagggcc tctacgaggg cactggccgg ctcttctttg
     5521 agttctaccg cctcctgcat gatgcgcggc ccaaggaggg agatgatcgc cccttcttct
     5581 ggctctttga gaatgtggtg gccatgggcg ttagtgacaa gagggacatc tcgcgatttc
     5641 tcgagtccaa ccctgtgatg attgatgcca aagaagtgtc agctgcacac agggcccgct
     5701 acttctgggg taaccttccc ggtatgaaca ggccgttggc atccactgtg aatgataagc
     5761 tggagctgca ggagtgtctg gagcatggca ggatagccaa gttcagcaaa gtgaggacca
     5821 ttactacgag gtcaaactcc ataaagcagg gcaaagacca gcattttcct gtcttcatga
     5881 atgagaaaga ggacatctta tggtgcactg aaatggaaag ggtatttggt ttcccagtcc
     5941 actatactga cgtgtccaac atgagccgct tggcgaggca gagactgctg ggccggtcat
     6001 ggagcgtgcc agtcatccgc cacctcttcg ctccgctgaa ggagtatttt gcgtgtgtgg
     6061 aggtgcacac caaccaagat ccgcttgatg ctgaagttca tacaaaccag gatcccctcg
     6121 atgccgaagt ccatactaat caggacccac tggacgcacc caagaaaaag cgcaaagtcg
     6181 gcagcggcgc ctgaggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     6241 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
     6301 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     6361 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     6421 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagtgcaaa gatggataaa
     6481 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
     6541 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     6601 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     6661 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     6721 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     6781 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     6841 aattacttcc actggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
     6901 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
     6961 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
     7021 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
     7081 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
     7141 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
     7201 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
     7261 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
     7321 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
     7381 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
     7441 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
     7501 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     7561 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     7621 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     7681 acagtggttc aaagtttttt tcttccattt caggtgtcgt gacgtacggc caccatgacc
     7741 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc
     7801 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac
     7861 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     7921 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     7981 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     8041 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     8101 tggttcctgg ccaccgtcgg cgtgtcgccc gaccaccagg gcaagggtct gggcagcgcc
     8161 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     8221 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     8281 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgatggaat
     8341 taattcgctg tctgcgaggg ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     8401 gacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     8461 cgcggtgatg cctttgaggg tggccgcgtc catctggtca gaaaagacaa tctttttgtt
     8521 gtcaagcttg aggtgtggca ggcttgagat ctggccatac acttgagtga caatgacatc
     8581 cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcaggt cgagataact
     8641 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     8701 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     8761 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8821 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaat gcgtcgagtg
     8881 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     8941 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     9001 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     9061 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     9121 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     9181 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     9241 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     9301 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     9361 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     9421 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     9481 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     9541 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     9601 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9661 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9721 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9781 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     9841 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9901 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9961 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    10021 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    10081 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    10141 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    10201 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    10261 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    10321 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    10381 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    10441 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    10501 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    10561 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    10621 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    10681 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    10741 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    10801 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    10861 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10921 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10981 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    11041 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    11101 tttccccgaa aagtgccacc tgacgtc
//
