LOCUS       Exported                5912 bp ds-DNA     circular SYN 08-SEP-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pEF1a-TALE-SunTag
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 8, 2017 from SnapGene 4.0.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        201..1458
                     /label=EF1a promoter
     CDS             1474..1563
                     /codon_start=1
                     /label=3X HA
                     /translation="YPYDVPDYAAYPYDVPDYAAYPYDVPDYAA"
     sig_peptide     1570..1590
                     /label=SV40 NLS
     CDS             1615..2022
                     /codon_start=1
                     /label=TALE N-term domain
                     /translation="VDLRTLGYSQQQQEKIKPKVRSTVAQHHEALVGHGFTHAHIVALS
                     QHPAALGTVAVKYQDMIAALPEATHEAIVGVGKQWSGARALEALLTVAGELRGPPLQLD
                     TGQLLKIAKRGGVTAVEAVHAWRNALTGAPLN"
     CDS             2074..2262
                     /codon_start=1
                     /label=TALE C-term domain
                     /translation="SIVAQLSRPDPALAALTNDHLVALACLGGRPALDAVKKGLPHAPA
                     LIKRTNRRIPERTSHRVA"
     CDS             2263..2364
                     /codon_start=1
                     /label=linker
                     /translation="GGGGSGGGGSQASPKKKRKVGRAKGGGGSGGGGS"
     misc_feature    2365..2421
                     /label=SunTag
     misc_feature    2437..2493
                     /label=SunTag
     misc_feature    2509..2565
                     /label=SunTag
     misc_feature    2581..2637
                     /label=SunTag
     misc_feature    2653..2709
                     /label=SunTag
     misc_feature    2725..2781
                     /label=SunTag
     misc_feature    2797..2853
                     /label=SunTag
     misc_feature    2869..2925
                     /label=SunTag
     misc_feature    2941..2997
                     /label=SunTag
     misc_feature    3013..3069
                     /label=SunTag
     intron          3086..3383
                     /label=IVS
     intron          3123..3352
     polyA_signal    3425..3663
                     /label=SV40 polyA
     rep_origin      4142..4761
                     /label=pBR322 Origin
     CDS             complement(4916..5776)
                     /codon_start=1
                     /label=Ampicillin
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        5818..5846
                     /label=Amp promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg tgcaaagatg gataaagttt taaacagaga ggaatctttg
      241 cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg
      301 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
      361 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
      421 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
      481 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
      541 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccactg gctgcagtac
      601 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
      661 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
      721 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
      781 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
      841 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
      901 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
      961 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     1021 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     1081 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     1141 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     1201 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     1261 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     1321 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     1381 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     1441 ccatttcagg tgtcgtgacg tacggccacc atgtacccct atgacgtgcc tgattacgcc
     1501 gcttatcctt acgatgtgcc cgactatgca gcctacccat acgatgtgcc cgactacgcc
     1561 gcaatggccc caaagaagaa gcggaaggtc ggtatccacg gagtcccagc agccgtagat
     1621 ttgagaactt tgggatattc acagcagcag caggaaaaga tcaagcccaa agtgaggtcg
     1681 acagtcgcgc agcatcacga agccctggtg ggtcatgggt ttacacatgc ccacatcgta
     1741 gccttgtcgc agcaccctgc agcccttggc acggtcgccg tcaagtacca ggacatgatt
     1801 gcggcgttgc cggaagccac acatgaggcg atcgtcggtg tggggaaaca gtggagcgga
     1861 gcccgcgccc ttgaggccct gttgacggtc gcgggagagc tgagagggcc tccccttcag
     1921 ctggacacgg gccagttgct gaagatcgcg aagcggggag gagtcacggc ggtcgaggcg
     1981 gtgcacgcgt ggcgcaatgc gctcacggga gcacccctca acctgacaga gacgctggct
     2041 gatgggcgtc tcggaggcag acccgcactg gagtcaattg tggcccagct ttcgaggccg
     2101 gaccccgcgc tggccgcact cactaatgat catcttgtag cgctggcctg cctcggcgga
     2161 cgacccgcct tggatgcggt gaagaagggg ctcccgcacg cgcctgcatt gattaagcgg
     2221 accaacagaa ggattcccga gaggacatca catcgagtgg caggtggcgg agggtctggg
     2281 ggaggcggta gtcaggccag cccgaaaaag aaaagaaaag tgggacgggc gaaaggaggc
     2341 ggtggctcag gcggtggggg aagcgaagaa cttttgagca agaattatca tcttgagaac
     2401 gaagtggctc gtcttaagaa aggttctggc agtggagaag aactgctttc aaagaattac
     2461 cacctggaaa atgaggtagc tagactgaaa aaggggagcg gaagtgggga ggagttgctg
     2521 agcaaaaatt atcatttgga gaacgaagta gcacgactaa agaaagggtc cggatcgggt
     2581 gaggagttac tctcgaaaaa ttatcatctc gaaaacgaag tggctcggct aaaaaagggc
     2641 agtggttctg gagaagagct attatctaaa aactaccacc tcgaaaatga ggtggcacgc
     2701 ttaaaaaagg gaagtggcag tggtgaagag ctactatcca agaattatca tcttgagaac
     2761 gaggtagcgc gtttgaagaa gggttccggc tcaggagagg aactgctctc gaagaactat
     2821 catcttgaaa atgaggtcgc tcgattaaaa aagggatcgg gcagtggtga ggaactactt
     2881 tcaaagaatt accacctcga aaacgaagta gctcgattaa agaaaggttc agggtcgggt
     2941 gaagaattac tgagtaaaaa ttatcatctg gaaaatgagg tagcgagact aaaaaagggg
     3001 agtggttctg gcgaggaatt gctatcgaaa aattatcatc ttgagaacga agttgctagg
     3061 ctcaaaaagt gagggggagg ctaactggaa ttaattcgct gtctgcgagg gccagctgtt
     3121 ggggtgagta ctccctctca aaagcgggca tgacttctgc gctaagattg tcagtttcca
     3181 aaaacgagga ggatttgata ttcacctggc ccgcggtgat gcctttgagg gtggccgcgt
     3241 ccatctggtc agaaaagaca atctttttgt tgtcaagctt gaggtgtggc aggcttgaga
     3301 tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca caggtgtcca
     3361 ctcccaggtc caactgcagg tcgatttaaa tgggaattcc acagcggccg catgtctaga
     3421 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     3481 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     3541 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     3601 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaatgcgtc
     3661 gagtgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     3721 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     3781 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     3841 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     3901 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3961 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4021 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4081 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4141 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4201 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4261 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4321 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
     4381 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4441 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4501 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4561 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     4621 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4681 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4741 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4801 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4861 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     4921 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     4981 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     5041 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     5101 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     5161 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     5221 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     5281 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     5341 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     5401 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     5461 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     5521 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     5581 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     5641 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     5701 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     5761 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     5821 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     5881 gcacatttcc ccgaaaagtg ccacctgacg tc
//
