LOCUS       Exported                6957 bp ds-DNA     circular SYN 12-SEP-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 12, 2017 from SnapGene 4.0.4
            http://www.snapgene.com
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
            Cat. No.631191, 631193,631187, 631189
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        209..1466
                     /label=EF1a
     misc_feature    1473..1478
                     /label=Kozak sequence
     CDS             1479..1481
                     /codon_start=1
                     /label=ATG
                     /translation="M"
     misc_feature    1485..1505
                     /label=SV40 NLS
     CDS             1506..2333
                     /codon_start=1
                     /label=scFv GCN4
                     /translation="GPDIVMTQSPSSLSASVGDRVTITCRSSTGAVTTSNYASWVQEKP
                     GKLFKGLIGGTNNRAPGVPSRFSGSLIGDKATLTISSLQPEDFATYFCALWYSNHWVFG
                     QGTKVELKRGGGGSGGGGSGGGGSSGGGSEVKLLESGGGLVQPGGSLKLSCAVSGFSLT
                     DYGVNWVRQAPGRGLEWIGVIWGDGITDYNSALKDRFIISKDNGKNTVYLQMSKVRSDD
                     TALYYCVTGLFDYWGQGTLVTVSSYPYDVPDYAGGGGGSGGGGSGGGGSGGGGS"
     misc_feature    2361..3071
                     /label=sfGFP
     misc_feature    3096..3260
                     /label=GB1
     misc_feature    3261..3314
                     /label=Linker
     CDS             3324..4028
                     /codon_start=1
                     /label=mCherry
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     misc_feature    3324..4028
                     /label=Amino acid translation
     misc_feature    4029..4127
                     /label=3x Ty1
     misc_feature    4131..4428
                     /label=IVS
     polyA_signal    4470..4708
                     /label=SV40 polyA
     rep_origin      5187..5806
                     /label=pBR322 Orgin
     CDS             complement(5961..6821)
                     /codon_start=1
                     /label=Ampicillin
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6863..6891)
                     /label=AMP promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ttaattaatg caaagatgga taaagtttta aacagagagg
      241 aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc
      301 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
      361 aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
      421 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
      481 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc
      541 cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccactggc
      601 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
      661 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
      721 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
      781 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
      841 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
      901 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
      961 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
     1021 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
     1081 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
     1141 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
     1201 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
     1261 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     1321 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     1381 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     1441 tttttcttcc atttcaggtg tcgtgacgta cggccaccat gggtcctaag aaaaagagga
     1501 aggtgggccc cgacatcgtg atgacccaga gccccagcag cctgagcgcc agcgtgggcg
     1561 accgcgtgac catcacctgc cgcagcagca ccggcgccgt gaccaccagc aactacgcca
     1621 gctgggtgca ggagaagccc ggcaagctgt tcaagggcct gatcggcggc accaacaacc
     1681 gcgcccccgg cgtgcccagc cgcttcagcg gcagcctgat cggcgacaag gccaccctga
     1741 ccatcagcag cctgcagccc gaggacttcg ccacctactt ctgcgccctg tggtacagca
     1801 accactgggt gttcggccag ggcaccaagg tggagctgaa gcgcggcggc ggcggcagcg
     1861 gcggcggcgg cagcggcggc ggcggcagca gcggcggcgg cagcgaggtg aagctgctgg
     1921 agagcggcgg cggcctggtg cagcccggcg gcagcctgaa gctgagctgc gccgtgagcg
     1981 gcttcagcct gaccgactac ggcgtgaact gggtgcgcca ggcccccggc cgcggcctgg
     2041 agtggatcgg cgtgatctgg ggcgacggca tcaccgacta caacagcgcc ctgaaggacc
     2101 gcttcatcat cagcaaggac aacggcaaga acaccgtgta cctgcagatg agcaaggtgc
     2161 gcagcgacga caccgccctg tactactgcg tgaccggcct gttcgactac tggggccagg
     2221 gcaccctggt gaccgtgagc agctacccat acgatgttcc agattacgct ggtggaggcg
     2281 gaggttctgg gggaggaggt agtggcggtg gtggttcagg aggcggcgga agcttggatc
     2341 caggtggagg tggaagcggt agcaaaggag aagaactttt cactggagtt gtcccaattc
     2401 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtccgtgga gagggtgaag
     2461 gtgatgctac aaacggaaaa ctcaccctta aatttatttg cactactgga aaactacctg
     2521 ttccgtggcc aacacttgtc actactctga cctatggtgt tcaatgcttt tcccgttatc
     2581 cggatcacat gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg
     2641 aacgcactat atctttcaaa gatgacggga cctacaagac gcgtgctgaa gtcaagtttg
     2701 aaggtgatac ccttgttaat cgtatcgagt taaagggtat tgattttaaa gaagatggaa
     2761 acattcttgg acacaaactc gagtacaact ttaactcaca caatgtatac atcacggcag
     2821 acaaacaaaa gaatggaatc aaagctaact tcaaaattcg ccacaacgtt gaagatggtt
     2881 ccgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
     2941 taccagacaa ccattacctg tcgacacaat ctgtcctttc gaaagatccc aacgaaaagc
     3001 gtgaccacat ggtccttctt gagtttgtaa ctgctgctgg gattacacat ggcatggatg
     3061 agctctacaa aggtggaggt cggaccggtg gcggtgagta caagcttatc ctgaacggta
     3121 aaaccctgaa aggtgaaacc accaccgaag ctgttgacgc tgctaccgcg gaaaaagttt
     3181 tcaaacagta cgctaacgac aacggtgttg acggtgaatg gacctacgac gacgctacca
     3241 aaaccttcac ggtaaccgaa agcgctggag ggggtggctc cggcggagga ggctctggtg
     3301 gcggagggag tggagcggcc gctgtgagca agggcgagga ggataacatg gccatcatca
     3361 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
     3421 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     3481 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct
     3541 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
     3601 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
     3661 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact
     3721 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc
     3781 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg
     3841 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
     3901 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca
     3961 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
     4021 tgtacaagga ggtgcacacc aaccaagatc cgcttgatgc tgaagttcat acaaaccagg
     4081 atcccctcga tgccgaagtc catactaatc aggacccact ggacgcatga tggaattaat
     4141 tcgctgtctg cgagggccag ctgttggggt gagtactccc tctcaaaagc gggcatgact
     4201 tctgcgctaa gattgtcagt ttccaaaaac gaggaggatt tgatattcac ctggcccgcg
     4261 gtgatgcctt tgagggtggc cgcgtccatc tggtcagaaa agacaatctt tttgttgtca
     4321 agcttgaggt gtggcaggct tgagatctgg ccatacactt gagtgacaat gacatccact
     4381 ttgcctttct ctccacaggt gtccactccc aggtccaact gcaggtcgat ttaaatggga
     4441 attccacagc ggccgcatgt ctagataact gatcataatc agccatacca catttgtaga
     4501 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     4561 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     4621 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     4681 actcatcaat gtatcttaat gcgtcgagtg cattctagtt gtggtttgtc caaactcatc
     4741 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     4801 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     4861 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     4921 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     4981 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     5041 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     5101 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     5161 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5221 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5281 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5341 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     5401 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5461 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5521 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5581 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5641 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5701 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5761 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     5821 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     5881 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     5941 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6001 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6061 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     6121 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6181 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     6241 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     6301 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     6361 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     6421 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     6481 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     6541 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     6601 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     6661 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     6721 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     6781 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     6841 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     6901 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
