LOCUS       Exported                6849 bp ds-DNA    circular SYN 01-MAR-2016
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pXPA-fapO-RgTALsyn
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   Jones
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2017 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6849
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        109..209
                     /note="pGAP"
                     /note="color: #ffffff"
     misc_feature    210..215
                     /note="Xba1+Nhe1"
                     /note="color: #a6acb3"
     protein_bind    216..244
                     /note="fapO1"
                     /note="color: #31849b"
     RBS             273..278
                     /note="RBS-1"
                     /note="color: #a6acb3"
     CDS             287..2368
                     /note="RgTALsyn"
                     /note="color: #993366; direction: RIGHT"
     terminator      2418..2842
                     /note="rrnB transcription terminator"
                     /note="color: #ffffff"
     rep_origin      2941..3388
                     /note="f1 ori"
                     /note="color: #ffff00"
     CDS             complement(3510..4370)
                     /note="Ap"
                     /note="B-lactamase gene"
                     /note="color: #993366; direction: LEFT"
     -10_signal      complement(4412..4418)
                     /note="-10 Ap"
                     /note="color: #a6acb3; direction: LEFT"
     -35_signal      complement(4436..4441)
                     /note="-35 Ap"
                     /note="color: #a6acb3; direction: LEFT"
     rep_origin      5131
                     /note="ColE1, pBR322 ori"
                     /note="color: #ffff00"
     CDS             complement(5562..5753)
                     /note="ROP"
                     /note="color: #993366; direction: LEFT"
ORIGIN
        1 ccatcggacg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt
       61 tgcctaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcatgcgc gtaatgctta
      121 ggcacaggat tgatttgtcg caatgattga cacgattccg cttgacgctg cgtaaggttt
      181 ttgtaatttt acaggcaacc ttttattcat ctagcactat tagtacctag tcttaattgt
      241 ccggtctaga aataattttg tttaacttta agaaggagat atacatatgg cgcctcgccc
      301 gacttcgcaa agccaggccc gcacttgccc gacgacgcag gttacccaag ttgatatcgt
      361 tgagaaaatg ttggcggctc ctactgatag cacgctggag ctggacggtt atagcctgaa
      421 tctgggtgat gtcgtgagcg ctgcgcgtaa gggtcgtcct gtccgtgtca aagatagcga
      481 tgaaatccgc agcaaaatcg acaagagcgt tgaattcctg cgcagccaac tgagcatgtc
      541 ggtttacggt gtgacgaccg gctttggcgg ctccgcggac acgcgcacgg aggacgcaat
      601 tagcctgcaa aaggcgttgc tggaacacca gctgtgtggt gtgttgccga gcagcttcga
      661 cagctttcgc ttgggtcgtg gtctggagaa tagcctgccg ttggaagtcg ttcgcggtgc
      721 aatgaccatt cgtgtgaatt cgctgacccg tggccatagc gctgttcgtc tggttgttct
      781 ggaagcactg acgaactttc tgaaccacgg tattaccccg attgttccgc tgcgcggtac
      841 gatctccgcg agcggcgatc tgtctccact gtcgtacatt gcagcggcga ttagcggtca
      901 cccggatagc aaagttcacg tggtccatga aggcaaagag aagatcctgt acgcgcgcga
      961 agcgatggcg ctgtttaacc tggagccggt ggttttgggt ccgaaggagg gcctgggtct
     1021 ggtgaatggt acggcagtct ccgcgagcat ggcaacgctg gcactgcacg acgcgcatat
     1081 gttgagcctg ttgagccaat cgctgaccgc gatgaccgtg gaggcgatgg tcggtcacgc
     1141 gggcagcttc catccattcc tgcacgatgt tacgcgtccg cacccgacgc aaatcgaggt
     1201 cgcgggtaac attcgcaaac tgctggaggg ctcgcgcttc gcggtccacc acgaggaaga
     1261 ggttaaggtc aaggatgatg aaggcatttt gcgtcaggat cgttatccgt tgcgcacgag
     1321 cccgcaatgg ttgggtccgc tggtgtccga cctgattcac gctcatgccg tcttgacgat
     1381 cgaagcgggt caaagcacca ccgataaccc actgatcgat gttgagaata agaccagcca
     1441 tcacggtggc aactttcaag cggcagcggt tgccaacacg atggaaaaga cccgtctggg
     1501 cttggcccaa atcggtaaac tgaatttcac ccagctgacg gagatgctga acgcgggcat
     1561 gaatcgtggc ttgccgagct gcctggcggc tgaagaccca tccctgagct atcattgcaa
     1621 aggtctggac attgcggcgg ctgcatatac gagcgaactg ggccacctgg ctaacccggt
     1681 caccacccac gtccaaccgg ctgaaatggc aaaccaggcg gtgaatagct tggcgttgat
     1741 tagcgcacgt cgtaccacgg aatctaacga cgttctgtcc ctgctgctgg caacgcacct
     1801 gtactgcgtg ctgcaggcga tcgacctgcg tgcgattgag ttcgagttca agaaacagtt
     1861 tggtcctgcc attgttagcc tgatcgacca acactttggt agcgcgatga cgggtagcaa
     1921 tctgcgtgat gagctggttg aaaaggtcaa taagactctg gccaagcgtt tggagcaaac
     1981 caatagctac gatctggttc cgcgctggca cgacgctttt agcttcgctg caggcactgt
     2041 tgtcgaggtt ctgtccagca cgagcctgag cttggcggcc gtgaacgcat ggaaggttgc
     2101 ggcagccgag agcgcgatct ccttgacgcg ccaggtccgt gaaacgtttt ggtccgctgc
     2161 aagcacctcc agcccggcgt tgtcttactt gagcccgcgc acgcagatcc tgtacgcatt
     2221 tgtgcgtgag gaactgggtg tcaaagcccg ccgtggtgac gtcttcttgg gtaaacaaga
     2281 agttaccatc ggcagcaacg ttagcaagat ttacgaagcc atcaagagcg gccgtatcaa
     2341 caatgttctg ctgaagatgc tggcataaac tagtcgcagc ttaattaacc taaactgctg
     2401 ccaccgctga gcaataactg ttttggcgga tgagagaaga ttttcagcct gatacagatt
     2461 aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg
     2521 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg
     2581 gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc
     2641 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
     2701 aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg
     2761 acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct
     2821 ttttgcgttt ctacaaactc ttttgtttat ttttctagct agcgaaaaat acattcaaat
     2881 atgtatccgc tcatgagaca ataacggagg agtcgactat atccggattg gcgaatggga
     2941 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     3001 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     3061 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     3121 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     3181 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     3241 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     3301 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     3361 cgcgaatttt aacaaaatat taacgtttac aatttctggc ggcacgatgg catgagatta
     3421 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3481 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3541 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3601 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3661 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3721 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3781 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3841 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3901 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3961 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     4021 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     4081 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     4141 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4201 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     4261 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4321 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     4381 tttcaatcat gattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     4441 atgtatttag aaaaataaac aaataggtca tgaccaaaat cccttaacgt gagttttcgt
     4501 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4561 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4621 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4681 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4741 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4801 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4861 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4921 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4981 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5041 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5101 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5161 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     5221 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     5281 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
     5341 cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga
     5401 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     5461 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     5521 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     5581 tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca
     5641 cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc
     5701 tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc
     5761 tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg
     5821 ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa
     5881 caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc
     5941 ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc
     6001 cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa
     6061 ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac
     6121 gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc
     6181 cgggtcctca acgacaggag cacgatcatg ctagtcatgc cccgcgccca ccggaaggag
     6241 ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc
     6301 taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6361 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag
     6421 ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc
     6481 ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt
     6541 gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac
     6601 cgagatgtcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc
     6661 catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat
     6721 ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat
     6781 ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact
     6841 taatgggcc
//
