LOCUS       Exported                7171 bp ds-DNA    circular SYN 04-AUG-2017
DEFINITION  pETDUET-1.
ACCESSION   .
VERSION     .
KEYWORDS    pETM6-RgTALsyn
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Bob Novy
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2017 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7171
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    5..25
                     /note="lacO"
                     /note="color: #31849b"
     RBS             58..63
                     /note="RBS-1"
                     /note="color: #a6acb3"
     CDS             72..2153
                     /note="RgTALsyn"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    complement(2189..2207)
                     /note="T7 Terminator Primer #69337-3"
                     /note="color: #a6acb3; direction: LEFT"
     terminator      2203..2250
                     /note="T7 terminator"
                     /note="color: #ffffff"
     misc_feature    2277..2296
                     /note="pETM6_MCS_REV"
                     /note="color: #a6acb3"
     rep_origin      2295..2742
                     /note="f1 ori"
                     /note="color: #ffff00"
     CDS             complement(2864..3724)
                     /note="Ap"
                     /note="B-lactamase gene"
                     /note="color: #993366; direction: LEFT"
     -10_signal      complement(3766..3772)
                     /note="-10 Ap"
                     /note="color: #a6acb3; direction: LEFT"
     -35_signal      complement(3790..3795)
                     /note="-35 Ap"
                     /note="color: #a6acb3; direction: LEFT"
     rep_origin      4485
                     /note="ColE1, pBR322 ori"
                     /note="color: #ffff00"
     CDS             complement(4916..5107)
                     /note="ROP"
                     /note="color: #993366; direction: LEFT"
     CDS             complement(5679..6761)
                     /note="lacI"
                     /note="lac repressor gene"
                     /note="color: #993366; direction: LEFT"
     misc_feature    7046..7064
                     /note="T7_Seq"
                     /note="color: #a6acb3"
     misc_feature    7104..7123
                     /note="pETM6_MCS_FOR"
                     /note="color: #a6acb3"
     misc_feature    7106..7124
                     /note="pET Upstream Primer #69214-3"
                     /note="color: #a6acb3"
     promoter        7155..7171
                     /note="T7 promoter"
                     /note="color: #ffffff"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatggcgcct cgcccgactt cgcaaagcca ggcccgcact tgcccgacga
      121 cgcaggttac ccaagttgat atcgttgaga aaatgttggc ggctcctact gatagcacgc
      181 tggagctgga cggttatagc ctgaatctgg gtgatgtcgt gagcgctgcg cgtaagggtc
      241 gtcctgtccg tgtcaaagat agcgatgaaa tccgcagcaa aatcgacaag agcgttgaat
      301 tcctgcgcag ccaactgagc atgtcggttt acggtgtgac gaccggcttt ggcggctccg
      361 cggacacgcg cacggaggac gcaattagcc tgcaaaaggc gttgctggaa caccagctgt
      421 gtggtgtgtt gccgagcagc ttcgacagct ttcgcttggg tcgtggtctg gagaatagcc
      481 tgccgttgga agtcgttcgc ggtgcaatga ccattcgtgt gaattcgctg acccgtggcc
      541 atagcgctgt tcgtctggtt gttctggaag cactgacgaa ctttctgaac cacggtatta
      601 ccccgattgt tccgctgcgc ggtacgatct ccgcgagcgg cgatctgtct ccactgtcgt
      661 acattgcagc ggcgattagc ggtcacccgg atagcaaagt tcacgtggtc catgaaggca
      721 aagagaagat cctgtacgcg cgcgaagcga tggcgctgtt taacctggag ccggtggttt
      781 tgggtccgaa ggagggcctg ggtctggtga atggtacggc agtctccgcg agcatggcaa
      841 cgctggcact gcacgacgcg cacatgttga gcctgttgag ccaatcgctg accgcgatga
      901 ccgtggaggc gatggtcggt cacgcgggca gcttccatcc attcctgcac gatgttacgc
      961 gtccgcaccc gacgcaaatc gaggtcgcgg gtaacattcg caaactgctg gagggctcgc
     1021 gcttcgcggt ccaccacgag gaagaggtta aggtcaagga tgatgaaggc attttgcgtc
     1081 aggatcgtta tccgttgcgc acgagcccgc aatggttggg tccgctggtg tccgacctga
     1141 ttcacgctca tgccgtcttg acgatcgaag cgggtcaaag caccaccgat aacccactga
     1201 tcgatgttga gaataagacc agccatcacg gtggcaactt tcaagcggca gcggttgcca
     1261 acacgatgga aaagacccgt ctgggcttgg cccaaatcgg taaactgaat ttcacccagc
     1321 tgacggagat gctgaacgcg ggcatgaatc gtggcttgcc gagctgcctg gcggctgaag
     1381 acccatccct gagctatcat tgcaaaggtc tggacattgc ggcggctgca tatacgagcg
     1441 aactgggcca cctggctaac ccggtcacca cccacgtcca accggctgaa atggcaaacc
     1501 aggcggtgaa tagcttggcg ttgattagcg cacgtcgtac cacggaatct aacgacgttc
     1561 tgtccctgct gctggcaacg cacctgtact gcgtgctgca ggcgatcgac ctgcgtgcga
     1621 ttgagttcga gttcaagaaa cagtttggtc ctgccattgt tagcctgatc gaccaacact
     1681 ttggtagcgc gatgacgggt agcaatctgc gtgatgagct ggttgaaaag gtcaataaga
     1741 ctctggccaa gcgtttggag caaaccaata gctacgatct ggttccgcgc tggcacgacg
     1801 cttttagctt cgctgcaggc actgttgtcg aggttctgtc cagcacgagc ctgagcttgg
     1861 cggccgtgaa cgcatggaag gttgcggcag ccgagagcgc gatctccttg acgcgccagg
     1921 tccgtgaaac gttttggtcc gctgcaagca cctccagccc ggcgttgtct tacttgagcc
     1981 cgcgcacgca gatcctgtac gcatttgtgc gtgaggaact gggtgtcaaa gcccgccgtg
     2041 gtgacgtctt cttgggtaaa caagaagtta ccatcggcag caacgttagc aagatttacg
     2101 aagccatcaa gagcggccgt atcaacaatg ttctgctgaa gatgctggca taaactagtc
     2161 gcagcttaat taacctaaac tgctgccacc gctgagcaat aactagcata accccttggg
     2221 gcctctaaac gggtcttgag gggttttttg ctagcgaaag gaggagtcga ctatatccgg
     2281 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     2341 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     2401 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     2461 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     2521 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     2581 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     2641 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     2701 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc tggcggcacg
     2761 atggcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     2821 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     2881 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     2941 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     3001 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     3061 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     3121 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     3181 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     3241 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     3301 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     3361 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     3421 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     3481 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     3541 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     3601 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     3661 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     3721 tcatactctt cctttttcaa tcatgattga agcatttatc agggttattg tctcatgagc
     3781 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gtcatgacca aaatccctta
     3841 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3901 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3961 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4021 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     4081 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4141 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4201 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4261 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4321 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4381 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4441 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4501 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     4561 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     4621 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     4681 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt
     4741 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     4801 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     4861 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     4921 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     4981 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     5041 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     5101 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     5161 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     5221 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     5281 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     5341 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     5401 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     5461 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     5521 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgctagtc atgccccgcg
     5581 cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg
     5641 cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg
     5701 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     5761 gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc
     5821 ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg
     5881 cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg
     5941 tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt aatggcgcgc
     6001 attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca
     6061 ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc
     6121 gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc
     6181 gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc
     6241 agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt
     6301 gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca
     6361 atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga
     6421 agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc
     6481 acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg
     6541 tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt
     6601 tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc
     6661 gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca
     6721 ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga
     6781 ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcc
     6841 gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt
     6901 gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag
     6961 tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa
     7021 gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc
     7081 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag cctaggatcg agatcgatct
     7141 cgatcccgcg aaattaatac gactcactat a
//
