LOCUS       Exported                9681 bp ds-DNA    circular SYN 04-AUG-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pETM6-RgTALsyn-HpaB-HpaC
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Jones
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2017 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     protein_bind    5..25
                     /note="lacO"
                     /note="color: #31849b"
     RBS             58..63
                     /note="RBS-1"
                     /note="color: #a6acb3"
     CDS             72..2153
                     /note="RgTALsyn"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    complement(2189..2207)
                     /note="T7 Terminator Primer #69337-3"
                     /note="color: #a6acb3; direction: LEFT"
     terminator      2203..2250
                     /note="T7 terminator"
                     /note="color: #ffffff"
     misc_feature    2251..2253
                     /note="pET Upstream Primer #69214-3"
                     /note="color: #a6acb3"
     misc_feature    2251..2252
                     /note="pETM6_MCS_FOR"
                     /note="color: #a6acb3"
     promoter        2284..2300
                     /note="T7 promoter"
                     /note="color: #ffffff"
     protein_bind    2305..2325
                     /note="lacO"
                     /note="color: #31849b"
     RBS             2358..2363
                     /note="RBS-1"
                     /note="color: #a6acb3"
     misc_feature    2372..3934
                     /note="HpaB"
                     /note="color: #a6acb3"
     misc_feature    complement(3969..3987)
                     /note="T7 Terminator Primer #69337-3"
                     /note="color: #a6acb3; direction: LEFT"
     terminator      3983..4030
                     /note="T7 terminator"
                     /note="color: #ffffff"
     misc_feature    4031..4033
                     /note="pET Upstream Primer #69214-3"
                     /note="color: #a6acb3"
     misc_feature    4031..4032
                     /note="pETM6_MCS_FOR"
                     /note="color: #a6acb3"
     promoter        4064..4080
                     /note="T7 promoter"
                     /note="color: #ffffff"
     protein_bind    4085..4105
                     /note="lacO"
                     /note="color: #31849b"
     RBS             4138..4143
                     /note="RBS-1"
                     /note="color: #a6acb3"
     misc_feature    4152..4664
                     /note="HpaC"
                     /note="color: #a6acb3"
     misc_feature    complement(4699..4717)
                     /note="T7 Terminator Primer #69337-3"
                     /note="color: #a6acb3; direction: LEFT"
     terminator      4713..4760
                     /note="T7 terminator"
                     /note="color: #ffffff"
     misc_feature    4787..4806
                     /note="pETM6_MCS_REV"
                     /note="color: #a6acb3"
     rep_origin      4805..5252
                     /note="f1 ori"
                     /note="color: #ffff00"
     CDS             complement(5374..6234)
                     /note="Ap"
                     /note="B-lactamase gene"
                     /note="color: #993366; direction: LEFT"
     -10_signal      complement(6276..6282)
                     /note="-10 Ap"
                     /note="color: #a6acb3; direction: LEFT"
     -35_signal      complement(6300..6305)
                     /note="-35 Ap"
                     /note="color: #a6acb3; direction: LEFT"
     rep_origin      6995
                     /note="ColE1, pBR322 ori"
                     /note="color: #ffff00"
     CDS             complement(7426..7617)
                     /note="ROP"
                     /note="color: #993366; direction: LEFT"
     CDS             complement(8189..9271)
                     /note="lacI"
                     /note="lac repressor gene"
                     /note="color: #993366; direction: LEFT"
     misc_feature    9556..9574
                     /note="T7_Seq"
                     /note="color: #a6acb3"
     misc_feature    9614..9633
                     /note="pETM6_MCS_FOR"
                     /note="color: #a6acb3"
     misc_feature    9616..9634
                     /note="pET Upstream Primer #69214-3"
                     /note="color: #a6acb3"
     promoter        9665..9681
                     /note="T7 promoter"
                     /note="color: #ffffff"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatggcgcct cgcccgactt cgcaaagcca ggcccgcact tgcccgacga
      121 cgcaggttac ccaagttgat atcgttgaga aaatgttggc ggctcctact gatagcacgc
      181 tggagctgga cggttatagc ctgaatctgg gtgatgtcgt gagcgctgcg cgtaagggtc
      241 gtcctgtccg tgtcaaagat agcgatgaaa tccgcagcaa aatcgacaag agcgttgaat
      301 tcctgcgcag ccaactgagc atgtcggttt acggtgtgac gaccggcttt ggcggctccg
      361 cggacacgcg cacggaggac gcaattagcc tgcaaaaggc gttgctggaa caccagctgt
      421 gtggtgtgtt gccgagcagc ttcgacagct ttcgcttggg tcgtggtctg gagaatagcc
      481 tgccgttgga agtcgttcgc ggtgcaatga ccattcgtgt gaattcgctg acccgtggcc
      541 atagcgctgt tcgtctggtt gttctggaag cactgacgaa ctttctgaac cacggtatta
      601 ccccgattgt tccgctgcgc ggtacgatct ccgcgagcgg cgatctgtct ccactgtcgt
      661 acattgcagc ggcgattagc ggtcacccgg atagcaaagt tcacgtggtc catgaaggca
      721 aagagaagat cctgtacgcg cgcgaagcga tggcgctgtt taacctggag ccggtggttt
      781 tgggtccgaa ggagggcctg ggtctggtga atggtacggc agtctccgcg agcatggcaa
      841 cgctggcact gcacgacgcg cacatgttga gcctgttgag ccaatcgctg accgcgatga
      901 ccgtggaggc gatggtcggt cacgcgggca gcttccatcc attcctgcac gatgttacgc
      961 gtccgcaccc gacgcaaatc gaggtcgcgg gtaacattcg caaactgctg gagggctcgc
     1021 gcttcgcggt ccaccacgag gaagaggtta aggtcaagga tgatgaaggc attttgcgtc
     1081 aggatcgtta tccgttgcgc acgagcccgc aatggttggg tccgctggtg tccgacctga
     1141 ttcacgctca tgccgtcttg acgatcgaag cgggtcaaag caccaccgat aacccactga
     1201 tcgatgttga gaataagacc agccatcacg gtggcaactt tcaagcggca gcggttgcca
     1261 acacgatgga aaagacccgt ctgggcttgg cccaaatcgg taaactgaat ttcacccagc
     1321 tgacggagat gctgaacgcg ggcatgaatc gtggcttgcc gagctgcctg gcggctgaag
     1381 acccatccct gagctatcat tgcaaaggtc tggacattgc ggcggctgca tatacgagcg
     1441 aactgggcca cctggctaac ccggtcacca cccacgtcca accggctgaa atggcaaacc
     1501 aggcggtgaa tagcttggcg ttgattagcg cacgtcgtac cacggaatct aacgacgttc
     1561 tgtccctgct gctggcaacg cacctgtact gcgtgctgca ggcgatcgac ctgcgtgcga
     1621 ttgagttcga gttcaagaaa cagtttggtc ctgccattgt tagcctgatc gaccaacact
     1681 ttggtagcgc gatgacgggt agcaatctgc gtgatgagct ggttgaaaag gtcaataaga
     1741 ctctggccaa gcgtttggag caaaccaata gctacgatct ggttccgcgc tggcacgacg
     1801 cttttagctt cgctgcaggc actgttgtcg aggttctgtc cagcacgagc ctgagcttgg
     1861 cggccgtgaa cgcatggaag gttgcggcag ccgagagcgc gatctccttg acgcgccagg
     1921 tccgtgaaac gttttggtcc gctgcaagca cctccagccc ggcgttgtct tacttgagcc
     1981 cgcgcacgca gatcctgtac gcatttgtgc gtgaggaact gggtgtcaaa gcccgccgtg
     2041 gtgacgtctt cttgggtaaa caagaagtta ccatcggcag caacgttagc aagatttacg
     2101 aagccatcaa gagcggccgt atcaacaatg ttctgctgaa gatgctggca taaactagtc
     2161 gcagcttaat taacctaaac tgctgccacc gctgagcaat aactagcata accccttggg
     2221 gcctctaaac gggtcttgag gggttttttg ctaggatcga gatcgatctc gatcccgcga
     2281 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
     2341 ttttgtttaa ctttaagaag gagatataca tatgaaacca gaagatttcc gcgccagtac
     2401 ccaacgtcct ttcaccgggg aagagtatct gaaaagcctg caggatggtc gcgagatcta
     2461 tatctatggc gagcgagtga aagacgtcac cactcatccg gcatttcgta atgcggcagc
     2521 gtctgttgcc cagctgtacg acgcactgca caaaccggag atgcaggact ctctgtgttg
     2581 gaacaccgac accggcagcg gcggctatac ccataaattc ttccgcgtgg cgaaaagtgc
     2641 cgacgacctg cgccagcaac gcgacgccat cgctgagtgg tcacgcctga gctatggctg
     2701 gatgggccgt accccagact acaaagccgc tttcggttgc gcactgggcg cgaatccggg
     2761 cttttacggt cagttcgagc agaacgcccg taactggtac acccgtattc aggaaactgg
     2821 cctctacttt aaccacgcga ttgttaaccc accgatcgat cgtcatttgc cgaccgataa
     2881 agtgaaagac gtttacatca agctggaaaa agagactgac gccgggatta tcgtcagcgg
     2941 tgcgaaagtg gttgccacca actcggcgct gactcactac aacatgattg gcttcggctc
     3001 ggcacaagtg atgggcgaaa acccggactt cgcactgatg ttcgttgcgc caatggatgc
     3061 cgatggcgtg aaattaatct cccgcgcctc ttatgagatg gtcgcgggtg ctaccggctc
     3121 gccatacgac tacccgctct ccagccgctt cgatgagaac gatgcgattc tggtgatgga
     3181 taacgtgctg attccatggg aaaacgtgct gatctaccgc gattttgatc gctgccgtcg
     3241 ctggacgatg gaaggcggtt ttgcccgtat gtatccgctg caagcctgtg tgcgcctggc
     3301 agtgaaatta gacttcatta cggcactgct gaaaaaatca ctcgaatgta ccggcaccct
     3361 ggagttccgt ggtgtgcagg ccgatctcgg tgaagtggta gcgtggcgca acaccttctg
     3421 ggcattgagt gactcgatgt gttcagaagc aacgccgtgg gtcaacgggg cttatttacc
     3481 ggatcatgcc gcactgcaaa cctatcgcgt actggcacca atggcctacg cgaagatcaa
     3541 aaacattatc gaacgcaacg ttaccagtgg cctgatctat ctcccttcca gtgcccgtga
     3601 cctgaataat ccgcagatcg accagtatct ggcgaagtat gtgcgcggtt cgaacggtat
     3661 ggatcacgtc cagcgcatca agatcctcaa actgatgtgg gatgctattg gcagcgaatt
     3721 tggtggtcgt cacgaactgt atgaaatcaa ctactccggt agccaggatg agattcgcct
     3781 gcagtgtctg cgccaggcac aaaactccgg caatatggac aagatgatgg cgatggttga
     3841 tcgctgcctg tcggaatacg accaggacgg ctggactgtg ccgcacctgc acaacaacga
     3901 cgatatcaac atgctggata agctgctgaa ataactagtc gcagcttaat taacctaaac
     3961 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
     4021 gggttttttg ctaggatcga gatcgatctc gatcccgcga aattaatacg actcactata
     4081 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
     4141 gagatataca tatgcaatta gatgaacaac gcctgcgctt tcgtgacgcg atggccagcc
     4201 tgtcggcagc ggtaaatatt atcaccaccg agggcgacgc cggacaatgc gggattacgg
     4261 caacggccgt ctgctcggtc acggatacac caccgtcgct gatggtgtgc attaacgcca
     4321 acagtgcgat gaacccggtt tttcagggca acggcaagtt gtgcgtcaac gtcctcaacc
     4381 atgagcagga actgatggca cgccacttcg cgggcatgac aggcatggcg atggaagagc
     4441 gttttagcct ctcatgctgg caaaaaggtc cgctggcgca gccggtgcta aaaggttcgc
     4501 tggccagtct tgaaggtgag atccgcgatg tgcaggcaat tggcacacat ctggtgtatc
     4561 tggtggagat taaaaacatc atcctcagtg cagaaggtca tggacttatc tactttaaac
     4621 gccgtttcca tccggtgatg ctggaaatgg aagctgcgat ttaactagtc gcagcttaat
     4681 taacctaaac tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
     4741 gggtcttgag gggttttttg ctagcgaaag gaggagtcga ctatatccgg attggcgaat
     4801 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4861 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4921 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4981 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     5041 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5101 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     5161 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5221 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc tggcggcacg atggcatgag
     5281 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5341 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     5401 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     5461 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     5521 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     5581 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     5641 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     5701 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     5761 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     5821 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     5881 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     5941 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6001 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6061 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6121 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6181 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6241 cctttttcaa tcatgattga agcatttatc agggttattg tctcatgagc ggatacatat
     6301 ttgaatgtat ttagaaaaat aaacaaatag gtcatgacca aaatccctta acgtgagttt
     6361 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6421 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6481 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6541 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6601 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6661 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6721 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6781 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6841 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6901 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6961 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7021 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7081 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     7141 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc
     7201 cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct
     7261 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
     7321 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     7381 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     7441 gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga
     7501 ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa
     7561 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     7621 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag
     7681 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg
     7741 taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca
     7801 gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca
     7861 gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg
     7921 gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct
     7981 tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc
     8041 tagccgggtc ctcaacgaca ggagcacgat catgctagtc atgccccgcg cccaccggaa
     8101 ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt
     8161 gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8221 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8281 ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct
     8341 ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct
     8401 gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca
     8461 ctaccgagat gtccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca
     8521 gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt
     8581 gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct
     8641 gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag
     8701 aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca
     8761 cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag
     8821 agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct
     8881 ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca
     8941 ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac
     9001 ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca
     9061 gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc
     9121 ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag
     9181 aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact
     9241 ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg
     9301 ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcc gggatctcga
     9361 cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg
     9421 agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc
     9481 acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc
     9541 cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg
     9601 gtgatgccgg ccacgatgcg tccggcgtag cctaggatcg agatcgatct cgatcccgcg
     9661 aaattaatac gactcactat a
//
