LOCUS       Exported               11168 bp ds-DNA     circular SYN 04-AUG-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    SnapGene DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2017 from SnapGene 4.0.1
            http://www.snapgene.com
COMMENT     Homologous recombination reaction between [bRA89 pgal delta pPGK1 
            Cacas9 HPH Bpl1 site.xdna] as a target sequence and 
            [PCR(pCORE.xdna)] as an inserted sequence.
FEATURES             Location/Qualifiers
     source          1..11168)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(253..487))
                     /label=TEF TER
                     /note="color: #0000ff; direction: LEFT"
     misc_feature    complement(1298..1665))
                     /label=TEF PRO
                     /note="color: #400080; direction: LEFT"
     misc_feature    2659..6916)
                     /label=CaCas9
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    6820..6843)
                     /label=NLS
                     /note="color: #ff00ff; direction: RIGHT"
     CDS             6886..6909)
                     /label=Flag tag
                     /note="color: #008000; direction: RIGHT"
     misc_feature    6916..7184)
                     /label=CYC1 terminator
                     /note="note=""direct strand 
                     translation""translation=""SLLESCN*"""
                     /note="color: #0000ff; direction: RIGHT"
     rep_origin      complement(7235..7690))
                     /direction=LEFT
                     /label=M13 origin
                     /note="color: #4682b4"
     rep_origin      7245..7685)
                     /direction=RIGHT
                     /label=F1 ori
                     /note="color: #4682b4"
     CDS             complement(7695..7763))
                     /label=LacZ alpha
                     /note="color: #6495ed; direction: LEFT"
     primer_bind     7834..7851)
                     /label=M13-fwd
                     /note="color: #008b8b; direction: RIGHT"
     primer_bind     7858..7877)
                     /label=T7
                     /note="color: #008b8b; direction: RIGHT"
     primer_bind     complement(8392..8411))
                     /label=T3
                     /note="color: #008b8b; direction: LEFT"
     primer_bind     complement(8429..8449))
                     /label=M13-rev
                     /note="color: #008b8b; direction: LEFT"
     misc_binding    complement(8455..8477))
                     /label=LacO
                     /note="color: #6495ed; direction: LEFT"
     rep_origin      complement(8817..9445))
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="color: #4682b4"
     CDS             complement(9597..10256))
                     /label=AmpR
                     /note="color: #8fbc8f; direction: LEFT"
     promoter        complement(10496..10524))
                     /label=Amp prom
                     /note="color: #4682b4; direction: LEFT"
     rep_origin      10586..11096)
                     /label=CEN-ARS pRS
                     /note="color: #ff6fcf"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcgaattc
      241 gagctcgttt aaactggatg gcggcgttag tatcgaatcg acagcagtat agcgaccagc
      301 attcacatac gattgacgca tgatattact ttctgcgcac ttaacttcgc atctgggcag
      361 atgatgtcga ggcgaaaaaa aatataaatc acgctaacat ttgattaaaa tagaacaact
      421 acaatataaa aaaactatac aaatgacaag ttcttgaaaa caagaatctt tttattgtca
      481 gtactgatta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
      541 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
      601 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
      661 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
      721 tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag acttgttcaa
      781 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
      841 gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
      901 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
      961 caggatattc ttctaatacc tggaatgctg ttttgccggg gatcgcagtg gtgagtaacc
     1021 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
     1081 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
     1141 tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt
     1201 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
     1261 atcgcggcct cgaaacgtga gtcttttcct tacccatggt tgtttatgtt cggatgtgat
     1321 gtgagaactg tatcctagca agattttaaa aggaagtata tgaaagaaga acctcagtgg
     1381 caaatcctaa ccttttatat ttctctacag gggcgcggcg tggggacaat tcaacgcgtc
     1441 tgtgagggga gcgtttccct gctcgcaggt ctgcagcgag gagccgtaat ttttgcttcg
     1501 cgccgtgcgg ccatcaaaat gtatggatgc aaatgattat acatggggat gtatgggcta
     1561 aatgtacggg cgacagtcac atcatgcccc tgagctgcgc acgtcaagac tgtcaaggag
     1621 ggtattctgg gcctccatgt cgctggccgg gtgacccggc ggggaaagta ccttcaaaga
     1681 atggggtctt atcttgtttt gcaagtacca ctgagcagga taataataga aatgataata
     1741 tactatagta gagataacgt cgatgacttc ccatactgta attgctttta gttgtgtatt
     1801 tttagtgtgc aagtttctgt aaatcgatta attttttttt ctttcctctt tttattaacc
     1861 ttaattttta ttttagattc ctgacttcaa ctcaagacgc acagatatta taacatctgc
     1921 ataataggca tttgcaagaa ttactcgtga gtaaggaaag agtgaggaac tatcgcatac
     1981 ctgcatttaa agatgccgat ttgggcgcga atcctttatt ttggcttcac cctcatacta
     2041 ttatcagggc cagaaaaagg aagtgtttcc ctccttcttg aattgatgtt accctcataa
     2101 agcacgtggc ctcttatcga gaaagaaatt accgtcgctc gtgatttgtt tgcaaaaaga
     2161 acaaaactga aaaaacccag acacgctcga cttcctgtct tcctattgat tgcagcttcc
     2221 aatttcgtca cacaacaagg tcctagcgac ggctcacagg ttttgtaaca agcaatcgaa
     2281 ggttctggaa tggcgggaaa gggtttagta ccacatgcta tgatgcccac tgtgatctcc
     2341 agagcaaagt tcgttcgatc gtactgttac tctctctctt tcaaacagaa ttgtccgaat
     2401 cgtgtgacaa caacagcctg ttctcacaca ctcttttctt ctaaccaagg gggtggttta
     2461 gtttagtaga acctcgtgaa acttacattt acatatatat aaacttgcat aaattggtca
     2521 atgcaagaaa tacatatttg gtcttttcta attcgtagtt tttcaagttc ttagatgctt
     2581 tctttttctc ttttttacag atcatcaagg aagtaattat ctacttttta caacaaatat
     2641 aaaacaccgg atctcaaaat ggataaaaag tatagtattg gtttagatat tggtactaac
     2701 tctgtgggtt gggcagttat cactgacgaa tataaagttc catcaaagaa atttaaggtg
     2761 ttaggtaaca ctgacagaca ctcaataaaa aagaatctta tcggtgctct tttgttcgac
     2821 tccggtgaaa ctgccgaggc tacacgttta aaaagaacag caagaagaag atatacccgt
     2881 agaaaaaata gaatatgtta tttacaagaa atcttttcta atgaaatggc taaagttgat
     2941 gattcctttt tccatagatt ggaagagtca tttttggttg aagaagacaa aaagcatgag
     3001 agacatccaa tctttgggaa tatagttgat gaagtggctt accatgaaaa atatcctacc
     3061 atttatcatt taagaaagaa attggtagat tcaactgata aagctgacct tagattaatc
     3121 tatttagcac ttgcccatat gattaaattt agaggtcatt ttttgattga aggtgatttg
     3181 aacccagata attctgacgt ggataaatta tttattcaat tagtccaaac ctacaaccaa
     3241 ttatttgagg aaaatccaat taatgctagt ggtgtcgatg ccaaagctat attatcagcc
     3301 agattatcaa aatctagacg tttggaaaat ttgattgccc aattgccagg agaaaaaaag
     3361 aatggattat ttggaaactt gatcgcatta tcattgggtt tgacaccaaa ttttaaatct
     3421 aattttgatt tagctgaaga tgctaaatta caattatcaa aagacaccta tgacgacgat
     3481 ttggacaatt tacttgctca aattggtgat caatatgcag atttgttctt agctgctaaa
     3541 aacttatctg atgctatttt gttgtctgat attttgagag tgaacacaga aataaccaaa
     3601 gctccattat cagcatctat gatcaaacgt tatgatgaac accatcagga tttgacttta
     3661 ttgaaagctt tggtgagaca acaattgcca gagaagtata aagaaatctt tttcgatcaa
     3721 tctaaaaacg ggtatgcagg ttatattgat gggggtgcct cccaagagga attttacaaa
     3781 tttataaaac ctattttaga aaagatggat gggactgagg aacttttggt caaattgaac
     3841 agagaagatt tgttacgtaa acagagaact tttgataatg gtagtatacc tcaccaaatt
     3901 catttgggtg agttgcatgc aattttaaga agacaagaag atttttatcc atttttaaaa
     3961 gataatagag aaaaaatcga gaaaatttta acctttagaa ttccatacta tgttgggcct
     4021 ttggctagag gtaattcaag atttgcctgg atgacacgta aatcagaaga aactattacc
     4081 ccttggaatt ttgaagaggt tgttgataaa ggagcatcag cacagagttt tattgaaaga
     4141 atgaccaatt tcgataaaaa cttaccaaat gaaaaagttt taccaaaaca ttccttgtta
     4201 tacgaatatt ttactgttta caatgaactt acaaaggtta aatatgttac tgaaggtatg
     4261 cgtaagccag cctttttatc tggagaacag aaaaaggcaa tagttgattt attgtttaaa
     4321 acaaatagaa aagttactgt taaacaatta aaagaagatt actttaagaa aattgaatgt
     4381 tttgattcag ttgaaatcag tggtgttgaa gacagattta atgctagttt aggaacttac
     4441 catgatttac ttaaaattat caaagataaa gatttcttgg ataacgaaga aaatgaagac
     4501 attttagaag acattgtttt aaccttaact ttattcgaag atagagagat gattgaagaa
     4561 cgtttgaaga cttatgcaca tttgtttgac gataaagtga tgaaacagtt gaaaagaaga
     4621 cgttatactg gatggggtag attgtctcgt aaattgatca atggaattag agataaacaa
     4681 agtggtaaaa ctatcttgga ctttttgaaa tctgacggat ttgctaatag aaatttcatg
     4741 caattgatcc acgacgatag tttgacattt aaagaagaca tccaaaaggc ccaagtgagt
     4801 gggcaaggtg attcattaca tgaacatatt gcaaatttag ccggatctcc tgctattaag
     4861 aaagggatat tacaaactgt taaagttgtg gatgaattag tgaaagtaat gggaagacat
     4921 aaacctgaaa acattgtcat tgagatggca agagaaaatc aaactacaca aaaaggacag
     4981 aaaaatagta gagaacgtat gaaaagaata gaagagggta ttaaagaatt gggtagtcaa
     5041 atattgaaag aacacccagt ggaaaatacc cagttgcaaa atgaaaaatt atatctttac
     5101 taccttcaaa atggacgtga tatgtatgtt gatcaggaat tagatataaa tagactttca
     5161 gattatgatg tagatcatat agttccacaa tctttcttga aagatgattc catagacaat
     5221 aaagtattaa ctagaagtga taaaaataga ggtaaaagtg ataatgtccc aagtgaggaa
     5281 gtcgtcaaaa agatgaaaaa ttactggcgt caacttttga atgctaaatt aattactcaa
     5341 agaaaatttg ataatttgac taaagcagaa agaggtgggc tttctgaatt agataaagcc
     5401 gggttcatta aaagacaatt ggtcgaaact agacaaatta ctaaacatgt tgcccaaatt
     5461 ttagattccc gtatgaacac taagtatgac gaaaatgata agttaatacg tgaggttaaa
     5521 gtcattactt taaaatcaaa acttgtctct gatttcagaa aggatttcca attctataaa
     5581 gttagagaaa ttaataatta tcatcatgct catgatgcat atttgaatgc tgtagttgga
     5641 actgctttaa tcaagaaata ccctaaatta gaatctgaat ttgtatatgg tgattacaaa
     5701 gtctatgatg ttagaaagat gattgctaaa tcagaacaag aaattggtaa agctacagct
     5761 aaatacttct tttactctaa cattatgaat ttctttaaaa cagaaattac tttggcaaac
     5821 ggtgaaatta gaaaaagacc tcttattgaa acaaatggtg agactggaga gatagtttgg
     5881 gacaaagggc gtgatttcgc tactgttaga aaagttttat caatgccaca agttaacatt
     5941 gtaaagaaaa cagaggttca aactggtggt ttctcaaaag aaagtatttt gcctaaaaga
     6001 aatagtgata aattgattgc cagaaaaaag gattgggatc caaagaaata tggtggtttc
     6061 gactcaccaa ccgtagccta ttctgttttg gttgtggcaa aggttgaaaa gggtaaaagt
     6121 aaaaagctta aatcagtaaa agaacttttg ggtattacaa taatggaaag aagttccttt
     6181 gaaaagaacc ctattgattt tttggaagct aaaggttata aggaagtaaa gaaggactta
     6241 ataatcaaat tgcctaaata ttctttattt gaattagaaa atgggagaaa aagaatgttg
     6301 gcttctgctg gagaattgca aaagggtaat gaattagcat tgccttccaa atatgttaac
     6361 ttcttgtatt tagcttcaca ctatgaaaag ttgaaagggt caccagaaga taacgagcaa
     6421 aaacaattat ttgttgaaca acacaaacac tacttagatg agattataga acaaattagt
     6481 gaattcagta aaagagtgat attagctgat gcaaatttag ataaagtttt gtcagcctat
     6541 aacaaacata gagataagcc aattagagaa caagcagaaa acattattca cttatttacc
     6601 cttaccaatt taggagcacc tgctgctttc aagtattttg atacaacaat tgatcgtaaa
     6661 agatatacct caacaaaaga agtcttagac gccaccttaa ttcatcaatc aatcactgga
     6721 ttgtatgaga caagaattga tttgtctcaa ttgggtggtg atgaaggggc tgatcctaag
     6781 aagaaaagaa aagttgatcc aaagaaaaag cgtaaggtgg atcctaagaa aaagagaaag
     6841 gttgactaca aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac
     6901 gatgacaagt gataatctct tctcgagtca tgtaattagt tatgtcacgc ttacattcac
     6961 gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg
     7021 tccctattta tttttttata gttatgttag tattaagaac gttatttata tttcaaattt
     7081 ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg
     7141 agaaggtttt gggacgctcg aaggctttaa tttgcggccg gtactgcggt gtgaaatacc
     7201 gcacagatgc gtaaggagaa aataccgcat caggaaattg taaacgttaa tattttgtta
     7261 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     7321 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     7381 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     7441 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     7501 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     7561 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     7621 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     7681 cagggcgcgt cgcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     7741 cgggcctctt cgctattacg ccagctggcg aaggggggat gtgctgcaag gcgattaagt
     7801 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa
     7861 tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc tagaactagt
     7921 ggatcccccg ggctgcagga attcgatatc aagctttctt tgaaaagata atgtatgatt
     7981 atgctttcac tcatatttat acagaaactt gatgttttct ttcgagtata tacaaggtga
     8041 ttacatgtac gtttgaagta caactctaga ttttgtagtg ccctcttggg ctagcggtaa
     8101 aggtgcgcat tttttcacac cctacaatgt tctgttcaaa agattttggt caaacgctgt
     8161 agaagtgaaa gttggtgcgc atgtttcggc gttcgaaact tctccgcagt gaaagataaa
     8221 tgatcctaga tgcgagatat cctcatgcct aggttttaga gctagaaata gcaagttaaa
     8281 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtggtg ctttttttgt
     8341 tttttatgtc taccgtcgac ctcgaggggg ggcccggtac ccagcttttg ttccctttag
     8401 tgagggttaa ttccgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     8461 tatccgctca caattccaca caacatagga gccggaagca taaagtgtaa agcctggggt
     8521 gcctaatgag tgaggtaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     8581 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     8641 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     8701 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     8761 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     8821 gcgttgctgg cgtttttcca taggctcggc ccccctgacg agcatcacaa aaatcgacgc
     8881 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt cccccctgga
     8941 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     9001 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     9061 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9121 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9181 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9241 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     9301 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9361 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9421 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9481 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9541 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     9601 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     9661 tgactgcccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     9721 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     9781 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9841 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9901 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9961 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgaaaaaa agcggttagc
    10021 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    10081 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10141 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10201 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10261 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10321 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10381 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10441 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10501 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10561 acatttcccc gaaaagtgcc acctgggtcc ttttcatcac gtgctataaa aataattata
    10621 atttaaattt tttaatataa atatataaat taaaaataga aagtaaaaaa agaaattaaa
    10681 gaaaaaatag tttttgtttt ccgaagatgt aaaagactct agggggatcg ccaacaaata
    10741 ctacctttta tcttgctctt cctgctctca ggtattaatg ccgaattgtt tcatcttgtc
    10801 tgtgtagaag accacacacg aaaatcctgt gattttacat tttacttatc gttaatcgaa
    10861 tgtatatcta tttaatctgc ttttcttgtc taataaatat atatgtaaag tacgcttttt
    10921 gttgaaattt tttaaacctt tgtttatttt tttttcttca ttccgtaact cttctacctt
    10981 ctttatttac tttctaaaat ccaaatacaa aacataaaaa taaataaaca cagagtaaat
    11041 tcccaaatta ttccatcatt aaaagatacg aggcgcgtgt aagttacagg caagcgatcc
    11101 gtcctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    11161 ctttcgtc
//
