LOCUS       Exported               10929 bp ds-DNA     circular SYN 08-SEP-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pGK-dCas9-SunTag-BFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 8, 2017 from SnapGene 4.0.4
            http://www.snapgene.com
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
            Cat. No.631191, 631193,631187, 631189
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(170..227)
                     /label=OL-585
     primer_bind     170..227
                     /label=OL-578
     promoter        209..712
                     /label=pPGK
     primer_bind     645..666
                     /label=OL-D152
     primer_bind     complement(688..743)
                     /label=OL 653
     primer_bind     688..743
                     /label=OL 652
     primer_bind     721..750
                     /label=OL-D62
     CDS             744..764
                     /codon_start=1
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    744..764
                     /label=Amino acid translation
     CDS             765..854
                     /codon_start=1
                     /label=3xHA
                     /translation="YPYDVPDYAAYPYDVPDYAAYPYDVPDYAA"
     misc_feature    855..860
                     /label=Amino acid translation
     misc_feature    861..5000
                     /label=Amino acid translation
     primer_bind     complement(866..884)
                     /label=OL-D211
     CDS             885..5000
                     /codon_start=1
                     /label=dCas9
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGDSRAD"
     variation       912..914
                     /label=D10A
     primer_bind     complement(1362..1397)
                     /label=OL 655
     variation       3402..3404
                     /label=H840A
     CDS             5001..5126
                     /codon_start=1
                     /label=Linker V3
                     /translation="GGGSGGGSPKKKRKVTGKKTSGQAGQASPKKKRKVGRAGGGS"
     misc_feature    5001..5126
                     /label=Amino acid translation
     sig_peptide     5025..5045
                     /label=SV40 NLS
     sig_peptide     5085..5105
                     /label=SV40 NLS
     misc_feature    5127..5183
                     /label=SunTag
     misc_feature    5127..5183
                     /label=SunTag
     misc_feature    5199..5255
                     /label=SunTag
     misc_feature    5271..5327
                     /label=SunTag
     misc_feature    5343..5399
                     /label=SunTag
     misc_feature    5415..5471
                     /label=SunTag
     misc_feature    5487..5543
                     /label=SunTag
     misc_feature    5559..5615
                     /label=SunTag
     misc_feature    5631..5687
                     /label=SunTag
     misc_feature    5703..5759
                     /label=SunTag
     misc_feature    5775..5831
                     /label=SunTag
     misc_feature    5856..5876
                     /label=SV40 NLS
     misc_feature    5883..6152
                     /label=HSV TK polyA
     primer_bind     5968..5989
                     /label=OL - D153
     promoter        6153..7410
                     /label=EF1a
     primer_bind     6593..6614
                     /label=OL -D154
     primer_bind     7205..7226
                     /label=OL- D155
     primer_bind     complement(7401..7425)
                     /label=OL-581
     CDS             7423..8133
                     /codon_start=1
                     /label=Tag-BFP2
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    8137..8434
                     /label=IVS
     primer_bind     8415..8474
                     /label=OL-584
     polyA_signal    8442..8680
                     /label=SV40 polyA
     rep_origin      9159..9778
                     /label=pBR322 Orgin
     CDS             complement(9933..10793)
                     /codon_start=1
                     /label=Ampicillin
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10835..10863)
                     /label=AMP promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ttaattaata ccgggtaggg gaggcgcttt tcccaaggca
      241 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
      301 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
      361 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
      421 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
      481 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
      541 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
      601 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
      661 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cggcggccgc
      721 acctgcagcc caagcttacc atgccaaaga agaagcggaa ggtctacccc tatgacgtgc
      781 ctgattacgc cgcttatcct tacgatgtgc ccgactatgc agcctaccca tacgatgtgc
      841 ccgactacgc cgcaatggcc ggtatccacg gagtcccagc agccatggac aagaagtact
      901 ccattgggct cgctatcggc acaaacagcg tcggctgggc cgtcattacg gacgagtaca
      961 aggtgccgag caaaaaattc aaagttctgg gcaataccga tcgccacagc ataaagaaga
     1021 acctcattgg cgccctcctg ttcgactccg gggagacggc cgaagccacg cggctcaaaa
     1081 gaacagcacg gcgcagatat acccgcagaa agaatcggat ctgctacctg caggagatct
     1141 ttagtaatga gatggctaag gtggatgact ctttcttcca taggctggag gagtcctttt
     1201 tggtggagga ggataaaaag cacgagcgcc acccaatctt tggcaatatc gtggacgagg
     1261 tggcgtacca tgaaaagtac ccaaccatat atcatctgag gaagaagctt gtagacagta
     1321 ctgataaggc tgacttgcgg ttgatctatc tcgcgctggc gcatatgatc aaatttcggg
     1381 gacacttcct catcgagggg gacctgaacc cagacaacag cgatgtcgac aaactcttta
     1441 tccaactggt tcagacttac aatcagcttt tcgaagagaa cccgatcaac gcatccggag
     1501 ttgacgccaa agcaatcctg agcgctaggc tgtccaaatc ccggcggctc gaaaacctca
     1561 tcgcacagct ccctggggag aagaagaacg gcctgtttgg taatcttatc gccctgtcac
     1621 tcgggctgac ccccaacttt aaatctaact tcgacctggc cgaagatgcc aagcttcaac
     1681 tgagcaaaga cacctacgat gatgatctcg acaatctgct ggcccagatc ggcgaccagt
     1741 acgcagacct ttttttggcg gcaaagaacc tgtcagacgc cattctgctg agtgatattc
     1801 tgcgagtgaa cacggagatc accaaagctc cgctgagcgc tagtatgatc aagcgctatg
     1861 atgagcacca ccaagacttg actttgctga aggcccttgt cagacagcaa ctgcctgaga
     1921 agtacaagga aattttcttc gatcagtcta aaaatggcta cgccggatac attgacggcg
     1981 gagcaagcca ggaggaattt tacaaattta ttaagcccat cttggaaaaa atggacggca
     2041 ccgaggagct gctggtaaag cttaacagag aagatctgtt gcgcaaacag cgcactttcg
     2101 acaatggaag catcccccac cagattcacc tgggcgaact gcacgctatc ctcaggcggc
     2161 aagaggattt ctaccccttt ttgaaagata acagggaaaa gattgagaaa atcctcacat
     2221 ttcggatacc ctactatgta ggccccctcg cccggggaaa ttccagattc gcgtggatga
     2281 ctcgcaaatc agaagagacc atcactccct ggaacttcga ggaagtcgtg gataaggggg
     2341 cctctgccca gtccttcatc gaaaggatga ctaactttga taaaaatctg cctaacgaaa
     2401 aggtgcttcc taaacactct ctgctgtacg agtacttcac agtttataac gagctcacca
     2461 aggtcaaata cgtcacagaa gggatgagaa agccagcatt cctgtctgga gagcagaaga
     2521 aagctatcgt ggacctcctc ttcaagacga accggaaagt taccgtgaaa cagctcaaag
     2581 aagactattt caaaaagatt gaatgtttcg actctgttga aatcagcgga gtggaggatc
     2641 gcttcaacgc atccctggga acgtatcacg atctcctgaa aatcattaaa gacaaggact
     2701 tcctggacaa tgaggagaac gaggacattc ttgaggacat tgtcctcacc cttacgttgt
     2761 ttgaagatag ggagatgatt gaagaacgct tgaaaactta cgctcatctc ttcgacgaca
     2821 aagtcatgaa acagctcaag aggcgccgat atacaggatg ggggcggctg tcaagaaaac
     2881 tgatcaatgg gatccgagac aagcagagtg gaaagacaat cctggatttt cttaagtccg
     2941 atggatttgc caaccggaac ttcatgcagt tgatccatga tgactctctc acctttaagg
     3001 aggacatcca gaaagcacaa gtttctggcc agggggacag tcttcacgag cacatcgcta
     3061 atcttgcagg tagcccagct atcaaaaagg gaatactgca gaccgttaag gtcgtggatg
     3121 aactcgtcaa agtaatggga aggcataagc ccgagaatat cgttatcgag atggcccgag
     3181 agaaccaaac tacccagaag ggacagaaga acagtaggga aaggatgaag aggattgaag
     3241 agggtataaa agaactgggg tcccaaatcc ttaaggaaca cccagttgaa aacacccagc
     3301 ttcagaatga gaagctctac ctgtactacc tgcagaacgg cagggacatg tacgtggatc
     3361 aggaactgga catcaatcgg ctctccgact acgacgtgga tgcaatcgtg ccccagtctt
     3421 ttctcaaaga tgattctatt gataataaag tgttgacaag atccgataaa aatagaggga
     3481 agagtgataa cgtcccctca gaagaagttg tcaagaaaat gaaaaattat tggcggcagc
     3541 tgctgaacgc caaactgatc acacaacgga agttcgataa tctgactaag gctgaacgag
     3601 gtggcctgtc tgagttggat aaagccggct tcatcaaaag gcagcttgtt gagacacgcc
     3661 agatcaccaa gcacgtggcc caaattctcg attcacgcat gaacaccaag tacgatgaaa
     3721 atgacaaact gattcgagag gtgaaagtta ttactctgaa gtctaagctg gtctcagatt
     3781 tcagaaagga ctttcagttt tataaggtga gagagatcaa caattaccac catgcgcatg
     3841 atgcctacct gaatgcagtg gtaggcactg cacttatcaa aaaatatccc aagcttgaat
     3901 ctgaatttgt ttacggagac tataaagtgt acgatgttag gaaaatgatc gcaaagtctg
     3961 agcaggaaat aggcaaggcc accgctaagt acttctttta cagcaatatt atgaattttt
     4021 tcaagaccga gattacactg gccaatggag agattcggaa gcgaccactt atcgaaacaa
     4081 acggagaaac aggagaaatc gtgtgggaca agggtaggga tttcgcgaca gtccggaagg
     4141 tcctgtccat gccgcaggtg aacatcgtta aaaagaccga agtacagacc ggaggcttct
     4201 ccaaggaaag tatcctcccg aaaaggaaca gcgacaagct gatcgcacgc aaaaaagatt
     4261 gggaccccaa gaaatacggc ggattcgatt ctcctacagt cgcttacagt gtactggttg
     4321 tggccaaagt ggagaaaggg aagtctaaaa aactcaaaag cgtcaaggaa ctgctgggca
     4381 tcacaatcat ggagcgatca agcttcgaaa aaaaccccat cgactttctc gaggcgaaag
     4441 gatataaaga ggtcaaaaaa gacctcatca ttaagcttcc caagtactct ctctttgagc
     4501 ttgaaaacgg ccggaaacga atgctcgcta gtgcgggcga gctgcagaaa ggtaacgagc
     4561 tggcactgcc ctctaaatac gttaatttct tgtatctggc cagccactat gaaaagctca
     4621 aagggtctcc cgaagataat gagcagaagc agctgttcgt ggaacaacac aaacactacc
     4681 ttgatgagat catcgagcaa ataagcgaat tctccaaaag agtgatcctc gccgacgcta
     4741 acctcgataa ggtgctttct gcttacaata agcacaggga taagcccatc agggagcagg
     4801 cagaaaacat tatccacttg tttactctga ccaacttggg cgcgcctgca gccttcaagt
     4861 acttcgacac caccatagac agaaagcggt acacctctac aaaggaggtc ctggacgcca
     4921 cactgattca tcagtcaatt acggggctct atgaaacaag aatcgacctc tctcagctcg
     4981 gtggagacag cagggctgac gggggaggat cagggggagg gtcccccaaa aagaaacgca
     5041 aagtgaccgg caagaaaacc agcggccagg ccggacaggc ctctcctaag aaaaaaagaa
     5101 aagtgggacg cgctggcggc ggaagcgaag aacttttgag caagaattat catcttgaga
     5161 acgaagtggc tcgtcttaag aaaggttctg gcagtggaga agaactgctt tcaaagaatt
     5221 accacctgga aaatgaggta gctagactga aaaaggggag cggaagtggg gaggagttgc
     5281 tgagcaaaaa ttatcatttg gagaacgaag tagcacgact aaagaaaggg tccggatcgg
     5341 gtgaggagtt actctcgaaa aattatcatc tcgaaaacga agtggctcgg ctaaaaaagg
     5401 gcagtggttc tggagaagag ctattatcta aaaactacca cctcgaaaat gaggtggcac
     5461 gcttaaaaaa gggaagtggc agtggtgaag agctactatc caagaattat catcttgaga
     5521 acgaggtagc gcgtttgaag aagggttccg gctcaggaga ggaactgctc tcgaagaact
     5581 atcatcttga aaatgaggtc gctcgattaa aaaagggatc gggcagtggt gaggaactac
     5641 tttcaaagaa ttaccacctc gaaaacgaag tagctcgatt aaagaaaggt tcagggtcgg
     5701 gtgaagaatt actgagtaaa aattatcatc tggaaaatga ggtagcgaga ctaaaaaagg
     5761 ggagtggttc tggcgaggaa ttgctatcga aaaattatca tcttgagaac gaagttgcta
     5821 ggctcaaaaa gggcggtggt tccggaggag gtagtccgaa gaagaaacgt aaggtcggat
     5881 gagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
     5941 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt
     6001 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
     6061 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
     6121 agccaacgtc ggggcggcag gccctgccat agtgcaaaga tggataaagt tttaaacaga
     6181 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
     6241 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     6301 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     6361 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     6421 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
     6481 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
     6541 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg
     6601 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc
     6661 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag
     6721 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt
     6781 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc
     6841 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca
     6901 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg
     6961 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga
     7021 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc
     7081 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc
     7141 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg
     7201 agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc
     7261 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg
     7321 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa
     7381 agtttttttc ttccatttca ggtgtcgtga cgtacggcca ccatggtgtc taagggcgaa
     7441 gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat
     7501 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga
     7561 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc
     7621 ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag
     7681 tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg
     7741 accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga
     7801 ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc
     7861 ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg
     7921 aagctcgtgg gcgggagcca tctgatcgca aacgccaaga ccacatatag atccaagaaa
     7981 cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc
     8041 aaggaggcca acaacgagac ctacgtcgag cagcacgagg tggcagtggc cagatactgc
     8101 gacctcccta gcaaactggg gcacaagctt aattgatgga attaattcgc tgtctgcgag
     8161 ggccagctgt tggggtgagt actccctctc aaaagcgggc atgacttctg cgctaagatt
     8221 gtcagtttcc aaaaacgagg aggatttgat attcacctgg cccgcggtga tgcctttgag
     8281 ggtggccgcg tccatctggt cagaaaagac aatctttttg ttgtcaagct tgaggtgtgg
     8341 caggcttgag atctggccat acacttgagt gacaatgaca tccactttgc ctttctctcc
     8401 acaggtgtcc actcccaggt ccaactgcag gtcgagataa ctgatcataa tcagccatac
     8461 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     8521 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     8581 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     8641 tggtttgtcc aaactcatca atgtatctta atgcgtcgag tgcattctag ttgtggtttg
     8701 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     8761 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     8821 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     8881 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     8941 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     9001 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     9061 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9121 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9181 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9241 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     9301 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     9361 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     9421 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     9481 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9541 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9601 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9661 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9721 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9781 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9841 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9901 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9961 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    10021 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    10081 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    10141 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    10201 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    10261 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    10321 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    10381 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    10441 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    10501 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    10561 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    10621 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    10681 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    10741 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    10801 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10861 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10921 cctgacgtc
//
