LOCUS       Exported                8694 bp ds-DNA     circular SYN 04-AUG-2017
DEFINITION  synthetic circular DNA
ACCESSION   pBbE7c-XAacxnT
VERSION     .
KEYWORDS    pBbE7c-XAacxnT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 4, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8694
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(7..1091)
                     /codon_start=1
                     /note="lacI"
                     /note="lacI has GTG start codon, this version does not have
                     internal EcoRV site"
                     /translation="CETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGA
                     ELHSQPRGTTTGGQTVVADWRCHLQSGPARAVANCRGD*ISRRSTGCQRGGVDGRTKRR
                     RSL*SGGAQSSRATRQWADH*LSAG*PGCHCCGSCLH*CSGVIS*CL*PDTHQQYYFLP
                     *RRYATGRGASGRIGSPANRAVSGPIKFCLGASASGWLA*ISHSQSNSADSGTGRRLEC
                     HVRFSTNHANAE*GHRSHCDAGCQRSDGAGRNARHYRVRAARWCGHLGSGIRRYRRQLM
                     LYPAVNHHQTGFSPAGANQRGPLAATLSGPGGEGQSAVARLTGEKKNHPGAQYANRLSP
                     RVGRFINAAGTTGFPTGKRAV"
     misc_feature    1481..1497
                     /note="T7 promoter"
                     /note="requires T7 RNA Polymerase"
     protein_bind    1500..1524
                     /note="lac operator"
     RBS             1541..1560
     CDS             1561..3714
                     /codon_start=1
                     /note="ACXAn"
                     /translation="MNVPVGHLRNVQVLGIDAGGTMTDTFFVDQDGDFVVGKAQSTPQN
                     EALGLIASSEDGLANWGMSLHEALAQLQTGVYSGTAMLNRVVQRKGLKCGLIVNRGMED
                     FHRMGRAVQSHLGYAYEDRIHLNTHRYDPPLVPRHLTRGVVERTDMIGTQVIPLREDTA
                     RDAARDLIAADAEGIVISLLHSYKNPENERRVRDIVLEEVEKSGKKIPVFASADYYPVR
                     KETHRTNTTILEGYAAEPSRQTLSKISNAFKERGTKFDFRVMATHGGTISWKAKELART
                     IVSGPIGGVIGAKYLGEVLGYKNIACSDIGGTSFDVALITQGEMTIKNDPDMARLVLSL
                     PLVAMDSVGAGAGSFIRLDPYTRAIKLGPDSAGYRVGVCWKESGIETVTISDCHMVLGY
                     LNPDNFLGGAVKLDRQRSVDAIKAQIADPLGLSVEDAAAGVIELLDSDLRDYLRSMISG
                     KGYSPASFVCFSYGGAGPVHTYGYTEGLGFEDVIVPAWAAGFSAFGCAAADFEYRYDKS
                     LDINMPTETPDTDKEKAAATLQAAWEELTKNVLEEFKLNGYSADQVTLQPGYRMQYRGQ
                     LNDLEIESPLAQAHTAADWDQLTDAFNATYGRVYAASARSPELGYSVTGAIMRGMVPIP
                     KPKIPKEPEEGETPPESAKIGTRKFYRKKRWVDAQLYHMESLRPGNRVMGPAVIESDAT
                     TFVVPDGFETWLDGHRLFHLREV"
     CDS             3895..6225
                     /codon_start=1
                     /note="ACXBn"
                     /translation="MNVTVDQSTLAGATRGIVRGGETLKEHRDRLMAATKATGRYAGLK
                     TLELREREPILYNKLFSRLRAGVVDARETAKKIAASPIVEQEGELCFTLYNAAGDSLLT
                     STGIIIHVGTMGAAIKYMIENNWEANPGVHDKDIFCNNDSLIGNVHPCDIHTIVPIFWE
                     GELIGWVGGVTHVIDTGAVGPGSMATGQVQRFGDGYSITCRKVGANDTLFRDWLHESQR
                     MVRTTRYWMLDERTRIAGCHMIRKLVEEVVAEEGIEAYWKFAYEAVEHGRLGLQARIKA
                     MTIPGTYRQVGFVDVPYAHEDVRVPSDFAKLDTIMHAPCEMTIRRDGTWRLDFEGSSRW
                     GWHTYNAHQVSFTSGIWVMMTQTLIPSEMINDGAAYGTEFRLPKGTWMNPDDRRVAFSY
                     SWHFLVSAWTALWRGLSRSYFGRGYLEEVNAGNANTSNWLQGGGFNQYDEIHAVNSFEC
                     AANGTGATAVQDGLSHAAAIWNPEGDMGDMEIWELAEPLVYLGRQIKASSGGSGKYRGG
                     CGFESLRMVWNAKDWTMFFMGNGHISSDWGLMGGYPAASGYRFAAHKTNLKELIASGAE
                     IPLGGDTDPENPTWDAMLPDAQIKRDKQAITTEEMFSDYDLYLNYMRGGPGFGDPLDRE
                     PQAVADDINGGYVLERFAGEVYGVVVRKGADGQYGVDEAGTAAARAQIRKDRLAKSVPV
                     SEWMKGEREKILAKDAGTQVRQMFAASFKLGPRFEKDFRTFWSLPDSWTLPEEEIGVPT
                     YGSRYSMDISELPDVHTVQFVEE"
     CDS             6307..6813
                     /codon_start=1
                     /note="ACXCn"
                     /translation="MAYTRSKIVDLVDGKIDPDTLHQMLSTPKDPERFVTYVEILQERM
                     PWDDKIILPLGPKLFIVQQKVSKKWTVRCECGHDFCDWKDNWKLSARVHVRDTPQKMEE
                     IYPRLMAPTPSWQVIREYFCPECGTLHDVEAPTPWYPVIHDFSPDIEGFYQEWLGLPVP
                     ERADA"
     misc_feature    6838..6966
                     /note="dbl term"
     rep_origin      complement(7100..7782)
                     /direction=LEFT
                     /note="colE1 origin"
     terminator      7788..7893
                     /note="T0"
     misc_feature    complement(7909..8568)
                     /note="CmR"
ORIGIN
        1 gacgtcctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
       61 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
      121 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
      181 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
      241 ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagatg tccgcaccaa
      301 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
      361 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
      421 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
      481 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
      541 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt
      601 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
      661 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
      721 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
      781 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
      841 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
      901 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
      961 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
     1021 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta
     1081 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
     1141 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc
     1201 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
     1261 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     1321 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     1381 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
     1441 ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg
     1501 gaattgtgag cggataacaa tttcagaatt caaaagatct tttaagaagg agatatacat
     1561 atgaacgttc ccgtgggaca cctgcgcaac gtccaggtgc ttggcatcga tgccggcggc
     1621 accatgacgg acactttctt cgtggaccag gacggcgact tcgtcgtcgg caaggcccag
     1681 tccacgcccc agaacgaggc tttgggcctc atcgcctcgt ctgaagacgg gctcgccaac
     1741 tggggcatgt cgctccacga ggcgctggcc cagcttcaga ccggtgtcta ttccggcacc
     1801 gcgatgctca atcgcgtggt ccagcgcaag gggctgaaat gcggcctcat cgtcaatcgc
     1861 ggcatggagg atttccaccg catgggccgc gcggtgcaga gccacctcgg ctacgcctat
     1921 gaggaccgca tccacctcaa cacccaccgc tacgatcccc cgctcgtccc gcgccacctc
     1981 acccgcggcg tggtggagcg caccgacatg atcggcacgc aggtgatccc gctgcgcgag
     2041 gacacggcgc gggacgccgc ccgcgacctg atcgcggccg acgcggaagg catcgtcatc
     2101 tcgctgctgc attcctacaa gaacccggag aacgagcgcc gcgtgcgcga catcgtgctg
     2161 gaggaagtgg agaagagcgg caagaagatc ccggtcttcg cctccgccga ttattatccg
     2221 gtgcgcaagg aaacccaccg caccaacacc accatcctcg aaggctacgc cgccgagccc
     2281 tcgcggcaga ccctgtccaa gatttccaac gccttcaagg agcgtggcac caagttcgac
     2341 ttccgcgtga tggcgaccca tggcggcacc atctcctgga aggcgaagga gctggcgcgc
     2401 accatcgtgt ctggccccat cggcggggtg atcggcgcga aatatctcgg cgaggtgctg
     2461 ggctacaaga acatcgcctg ctcggacatc ggcggcacct cgttcgacgt ggcgctgatc
     2521 acccagggcg agatgaccat caagaacgat ccggacatgg cgcgcctcgt cctgtcgctg
     2581 ccgctggtgg ccatggattc ggtgggcgcc ggcgccggct ccttcattcg gctcgatccc
     2641 tatacccggg ccatcaagct ggggccggat tcggccggct accgggtggg cgtgtgctgg
     2701 aaggagagcg gcatcgagac ggtgaccatc tccgactgtc acatggtgct cggctatctc
     2761 aaccccgaca acttcctcgg cggggcggtg aagctcgatc gccagcgctc ggtggatgcc
     2821 atcaaggcgc agattgccga tccgctcggc ctgtcggtcg aggatgccgc cgccggcgtt
     2881 atcgaactgc tcgacagcga cctgcgcgac tacctgcgct ccatgatctc cggcaagggc
     2941 tattcgccgg cgagcttcgt gtgcttctcc tatggcggcg ccggcccggt gcacacctat
     3001 ggctacaccg agggccttgg cttcgaggat gtgatcgttc cggcctgggc ggcgggcttc
     3061 tccgccttcg gctgcgcggc ggcggatttc gagtatcgct acgacaagtc cctcgacatc
     3121 aacatgccga cggagacccc tgacaccgac aaggagaagg ccgccgccac cctccaggcg
     3181 gcatgggagg aactcaccaa gaacgtgctc gaagagttca agctgaacgg ctattcggcc
     3241 gaccaggtga cgctccagcc cggctaccgc atgcagtatc gcgggcagct caacgacctg
     3301 gagatagaga gcccgctggc gcaggcccac accgcggctg actgggacca gctgacggac
     3361 gccttcaacg ccacctatgg ccgggtctat gccgcctccg cccgctcgcc ggagctgggc
     3421 tattcggtta ccggcgccat catgcgcggc atggtgccga tccccaagcc caagatcccc
     3481 aaggagccgg aggagggcga gaccccgccg gagagcgcca agatcggcac ccgcaagttc
     3541 tatcgcaaga agcggtgggt ggatgcgcag ctctatcaca tggagtcgct gcgccccggc
     3601 aaccgggtga tgggccctgc cgtgatcgaa tccgacgcca ccaccttcgt ggtgcccgac
     3661 ggcttcgaga cctggctcga cggccaccgc ctgttccacc tgcgcgaggt gtgagggcag
     3721 gtcaggacgg gccgtcatgc ccgggcttgt cccgggcatc cacaccgtgc cgctcgcgca
     3781 acgctctgaa tgctgctccc agccgcgagg cgtggatggc cgggacaagc ccggccatga
     3841 cgacgcgaag aagcacgctc aatccgagtc aacgaactca aaggaaacga ccccatgaac
     3901 gtgactgttg accagagcac ccttgcgggt gcgacgcgcg gcatcgtgcg cggcggcgag
     3961 acgctgaagg agcatcgcga ccgcctcatg gccgccacca aggccaccgg ccgctatgcc
     4021 ggcctgaaga cgctggagct gcgcgagcgc gagcccatcc tctacaacaa gctgttctcg
     4081 cgcctgcggg ccggcgtggt ggatgcccgc gagaccgcca agaagatcgc cgcctccccc
     4141 atcgtggagc aggagggcga gctgtgcttc accctctaca acgcggcggg cgacagcctt
     4201 ctcacctcca ccggcatcat catccatgtg ggaaccatgg gtgccgcgat caaatacatg
     4261 atcgagaaca attgggaggc caatcccggc gtccacgaca aggacatctt ctgcaacaac
     4321 gacagcctga tcggaaacgt ccatccgtgc gacatccaca ccatcgtgcc catcttctgg
     4381 gaaggtgagc tgatcggctg ggtgggcggc gtcacccacg tcatcgacac cggcgcggtg
     4441 gggccgggct ccatggccac cgggcaggtg cagcgcttcg gcgacggcta ttccatcacc
     4501 tgccgcaagg tgggcgccaa cgacaccctg ttccgcgact ggctgcacga aagccagcgc
     4561 atggtgcgca ccacccgcta ctggatgctg gacgagcgca cccgcatcgc cggctgccac
     4621 atgatccgca agctggtgga agaggtggtc gcggaagagg gcatcgaggc ctactggaag
     4681 ttcgcctacg aggcggtgga gcacggccgg ctcgggctcc aggcccgcat caaggccatg
     4741 accatccccg gcacctaccg gcaggtgggc ttcgtggatg tgccctacgc ccatgaggac
     4801 gtgcgggtgc cctccgattt cgccaagctc gacaccatca tgcacgcccc gtgcgagatg
     4861 acgatccgcc gggacggcac ctggcgcctc gacttcgagg gatcgagccg ctggggctgg
     4921 cacacctaca acgcccacca ggtgagcttc acctcgggca tctgggtgat gatgacccag
     4981 accctcatcc cctccgagat gatcaatgac ggcgccgcct acggcaccga gttccggctg
     5041 cccaagggca cctggatgaa cccggacgac cgccgcgtcg ccttctccta cagctggcac
     5101 ttcctggtct cggcctggac ggcgctgtgg cggggcctgt cgcgctccta cttcgggcgc
     5161 ggctacctgg aggaggtcaa cgccggcaac gccaacacct ccaactggct gcagggcggc
     5221 ggcttcaacc agtatgacga gatccacgcc gtgaactcgt tcgagtgcgc cgccaacggc
     5281 accggcgcca ccgcggtgca ggacggcctg tcccacgccg ccgccatctg gaacccggaa
     5341 ggcgacatgg gtgacatgga gatctgggaa ctggccgagc cgctggtcta tctcggccgg
     5401 cagatcaagg cgtcgtcggg cggctccggc aagtatcgcg gcggctgcgg cttcgagagc
     5461 ctgcgcatgg tctggaacgc caaggactgg accatgttct tcatgggcaa cggccacatc
     5521 tcctccgact gggggctgat gggcggttat ccggcggcct ccggctatcg cttcgccgcc
     5581 cacaagacca acctgaagga actgatcgcg tccggcgcgg agatcccgct cggcggcgac
     5641 accgatccgg agaacccgac ctgggacgcc atgctgcccg acgcccagat caagcgcgac
     5701 aagcaggcca tcaccaccga ggagatgttc tccgactacg acctctacct caactacatg
     5761 cgcggcggcc ccggcttcgg tgatccgctc gaccgtgagc cgcaggcggt ggccgacgac
     5821 atcaatggcg gctacgtgct ggagcgcttc gccggcgagg tctatggcgt cgtggtgcgg
     5881 aagggcgcgg acggccagta tggcgtggac gaggccggca ccgccgccgc ccgcgcgcag
     5941 atccgcaagg atcgcctcgc caagtcagtg ccggtctccg aatggatgaa gggcgagcgc
     6001 gagaagatcc tcgccaagga tgcgggcacg caggtgcggc agatgtttgc cgcctccttc
     6061 aagctcggcc cgcgcttcga gaaggacttc cgcaccttct ggagcctgcc cgacagctgg
     6121 accctgccgg aggaagaaat cggcgtgccc acctacggct cgcgctattc catggacatc
     6181 tccgaactgc ccgacgtgca caccgtgcag ttcgtggagg aatgatccct ccactgggcg
     6241 gcggcgacag ccgtcgctgc cgcccatttt ttccttggaa aacaaacatc aagtggagga
     6301 aacaccatgg cctatacccg ctcgaagatc gtcgatctcg tggacggcaa gatcgatccc
     6361 gatacgctgc accagatgct ctccaccccc aaggacccgg agcgcttcgt gacctatgtg
     6421 gagatcctgc aggagcgcat gccgtgggat gacaagatca tcctgccgct cggccccaag
     6481 ctcttcatcg tgcagcagaa agtctccaag aaatggaccg tgcgctgcga atgcggccac
     6541 gatttctgcg actggaagga caattggaag ctcagcgccc gcgtccatgt tcgcgacacg
     6601 ccgcagaaga tggaggagat ctatccccgc ctcatggcgc ccaccccgtc ctggcaggtg
     6661 atccgggagt atttctgccc cgagtgcggc acccttcacg atgtggaggc gcccaccccc
     6721 tggtatccgg tgatccacga tttctcgccg gatatcgagg gcttctacca ggagtggctc
     6781 ggcctgccgg tgcccgagcg ggccgacgcc tgaggatcca aactcgagta aggatctcca
     6841 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     6901 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     6961 tttataccta gggcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7021 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7081 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7141 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7201 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7261 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7321 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7381 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7441 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7501 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     7561 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7621 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7681 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7741 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gactagtgct tggattctca
     7801 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
     7861 gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc
     7921 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
     7981 acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
     8041 atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
     8101 aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc
     8161 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
     8221 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc
     8281 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
     8341 tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
     8401 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
     8461 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
     8521 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
     8581 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
     8641 gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaga tatc
//
