LOCUS Exported 11536 bp ds-DNA circular SYN 08-FEB-2018 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11536) AUTHORS . TITLE Direct Submission JOURNAL Exported Feb 8, 2018 from SnapGene 4.1.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11536 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 17..520 /label=CEN/ARS /note="S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence" promoter 557..661 /gene="bla" /label=AmpR promoter CDS 662..1522 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1693..2281 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" terminator 2380..2610 /label=35S terminator CDS complement(2617..2987) /codon_start=1 /label=BlastR /translation="ADREGYGDD*LDSYLRGLLGGFRCSVVRRTDLHGGQRVPFYGGAL RGAGGPGDRRCCRGWEPDVHCRHREREPRDSEPLRAVPAGPARPPPWDQGDCEGFRRAA YGGRYQRTSPFGLRVGERL" promoter 2988..3724 /label=LDSP promoter terminator 3742..4408 /label=LDSP terminator CDS complement(4418..4438) /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(4454..4480) /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS complement(4481..4996) /codon_start=1 /label=Nlux /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW RLCERILAG" CDS complement(5021..9133) /codon_start=1 /label=Cas9 /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGDSRAD" CDS complement(9134..9154) /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=SV40 NLS /translation="PKKKRKV" promoter 9165..10348 /label=Ribi misc_feature 10377..10456 /label=sgRNA misc_feature 10457..10529 /label=HDV terminator 10536..11536 /label=CS terminator ORIGIN 1 accggtttct tagacggatc gcttgcctgt aacttacacg cgcctcgtat cttttaatga 61 tggaataatt tgggaattta ctctgtgttt atttattttt atgttttgta tttggatttt 121 agaaagtaaa taaagaaggt agaagagtta cggaatgaag aaaaaaaaat aaacaaaggt 181 ttaaaaaatt tcaacaaaaa gcgtacttta catatatatt tattagacaa gaaaagcaga 241 ttaaatagat atacattcga ttaacgataa gtaaaatgta aaatcacagg attttcgtgt 301 gtggtcttct acacagacaa gatgaaacaa ttcggcatta atacctgaga gcaggaagta 361 caagataaaa ggtagtattt gttggcgatc cccctagagt cttttacatc ttcggaaaac 421 aaaaactatt ttttctttaa tttctttttt tactttctat ttttaattta tatatttata 481 ttaaaaaatt taaattataa ttatttttat agcacgtgat gaaaaggacc caggtggcac 541 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 601 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 661 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc 721 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc 781 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc 841 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc 901 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt 961 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt 1021 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat 1081 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct 1141 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat 1201 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc 1261 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg 1321 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc 1381 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta 1441 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc 1501 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga 1561 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 1621 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat 1681 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 1741 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 1801 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt 1861 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 1921 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 1981 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 2041 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 2101 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 2161 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 2221 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 2281 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat 2341 gttctttcct gcgttatccc ctgattctgt ggtttaaacc aggtcactgg attttggttt 2401 taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat actaagggtt 2461 tcttatatgc tcaacacatg agcgaaaccc tataagaacc ctaattccct tatctgggaa 2521 ctactcacac attattctgg agaaaaatag agagagatag atttgtagag agagactggt 2581 gatttttgcg gactccggtc ggcatctact actagttcaa gcgctctccc acacgtagcc 2641 cgaagggaga agttctctga taccgaccgc cgtaggctgc ccgtcggaat ccttcacaat 2701 cgccttgatc ccagggtgga ggtcgagcag gacctgccgg caccgcccgc aggggctcag 2761 aatcccgcgg ttctcgttcc cgatggcgac aatgcacgtc aggttcccag ccgcggcagc 2821 agcggcggtc cccaggacca ccagctccgc gcaaggcccc cccgtaaaat ggtacacgtt 2881 gacccccgtg aagatccgtc cgtcggacga cagagcagcg gaagccaccg agtaatcctc 2941 ggagatagga atcgagttaa tcgtcgccgt agccctctcg atcagcgtgc tctcctcctg 3001 cgagaggggc atgggcccct cgagcttatc ttgagataga atttgtgcta gtgtgtctac 3061 gatgtgttac agtgcgagcg ccgtgagagg gtgcgtggca tcagattcca ttcctaattc 3121 ggactgacgc cgtacacagg aaaacggcaa gcaacccaaa caaccacatc ctatcaaatg 3181 caatacacga aatggtccag catgtcaaac acgtaaaaat cgttgtgtgg actgctcgta 3241 gagaccgtag ccgtcaaaaa gacgcatatc acggtcaaat cttgtgagtt ctccaagaac 3301 aaacggccag gttggctgtc gagtcgaagc acagaaagat gctcgtcaca agccgcctcc 3361 ccatgtctga cgctaccaaa aacttggtat tccgtggtca agtccttgac ccgccccggc 3421 agaaatgcag ggcaaaattt ataccagaca aggccactct gccgcgtacg gtggtcgtta 3481 cgcacatggc ttcgtcacag ttgcccggtg ctttgtcgcc tatggcagcg aacatgacgc 3541 aagcgggtct caacggccga cgagcgtctg cgcgagaaat gagcaaatag accgcaccca 3601 cccatcacaa caacgcgcgc gggcgaataa atgcatggcc cggacgttct ggggtggggt 3661 gctgttggca ggtgggaggg actgtattgt cttgaggatg gatgttgact tgatttggta 3721 cacagcggcc gccatggcct ctagtggatc agcttgcatg cctgcaggag acgatatcac 3781 ctcttctgtt tccacgataa aaatagactg ctcatttctt cgtcgtcttc atcgtctgct 3841 ttttctgctt cgcctctgtc tggggtctga aaccactaca cacacacaca acactcgtac 3901 tcccactttc acaaaagcgt aagctcaccg gcttttctta cacgtacatt ttagtggatc 3961 ccatcacgcc actaccacgc ccgcggggga tggaacggag gggagagaga gaagggggaa 4021 gcatggatga atgagacatt gagggaaagg aggggaggga gcagtccatc agggcgctac 4081 ctctcttgtc ccccaaaccc tgttgagccg ttcaacatgt ttcatgtttc ctccctcccc 4141 ccttccctcc ctggcctttc cgcggagcca ttcaagtgac gtctggaccg caccgtaaca 4201 aaatcgtttc tatggggggt ttgtttgaca accacgtctt cagcgttttt aaaaaaaaaa 4261 gcgggcaagc cctctcaccc tcactcatgc ccatcctcct cctctcctgc ggaacattct 4321 tacaaaaggc gtaactcgac gacaactcaa agaacgacaa acatcaatcc caaaaaaaaa 4381 atctctactc gtctctcttg gatctttcca attgtcagac cttcctcttc tttttcgggc 4441 tagccaccat ggacgcgtag tcgggcacgt cgtaggggta tccggccagg atgcgctcgc 4501 acaggcgcca gccggttacc ccgttgatgg tcacgcggaa caacagcgag ccatccgggt 4561 tgatcagccg ctcgtcaatg atcttattgc cgttccacag ggtgccggtc accgtgatct 4621 tcttgccgtc gaacacggca atgccctcgt aaggacggcc gaagtagtca atcatgttcg 4681 gcgtcacacc gtcgatgacc agcgtcccgt agtgcagtat caccttgaag tggtgatcgt 4741 ccacggggta cacaaccttg aagatcttct cgatctggcc catttggtcc ccgctcaggc 4801 cctcgtaggg aatgatgacg tggatgtcga tcttcaggcc gttctcgccg ctcaggacga 4861 tgcgctggat tggggtcacg gagacgccca ggttctggaa aagcgaggac acaccgccct 4921 gctccagcac ctggtcgagg ttatagccag ctgtctgccg ccagtcgccc acgaagtcct 4981 cgagagtaaa caccatgtta acggacccgc tgccggaccc gtcagccctg ctgtctccac 5041 cgagctgaga gaggtcgatt cttgtttcat agagccccgt aattgactga tgaatcagtg 5101 tggcgtccag gacctccttt gtagaggtgt accgctttct gtctatggtg gtgtcgaagt 5161 acttgaaggc tgcaggcgcg cccaagttgg tcagagtaaa caagtggata atgttttctg 5221 cctgctccct gatgggctta tccctgtgct tattgtaagc agaaagcacc ttatcgaggt 5281 tagcgtcggc gaggatcact cttttggaga attcgcttat ttgctcgatg atctcatcaa 5341 ggtagtgttt gtgttgttcc acgaacagct gcttctgctc attatcttcg ggagaccctt 5401 tgagcttttc atagtggctg gccagataca agaaattaac gtatttagag ggcagtgcca 5461 gctcgttacc tttctgcagc tcgcccgcac tagcgagcat tcgtttccgg ccgttttcaa 5521 gctcaaagag agagtacttg ggaagcttaa tgatgaggtc ttttttgacc tctttatatc 5581 ctttcgcctc gagaaagtcg atggggtttt tttcgaagct tgatcgctcc atgattgtga 5641 tgcccagcag ttccttgacg cttttgagtt ttttagactt ccctttctcc actttggcca 5701 caaccagtac actgtaagcg actgtaggag aatcgaatcc gccgtatttc ttggggtccc 5761 aatctttttt gcgtgcgatc agcttgtcgc tgttcctttt cgggaggata ctttccttgg 5821 agaagcctcc ggtctgtact tcggtctttt taacgatgtt cacctgcggc atggacagga 5881 ccttccggac tgtcgcgaaa tccctaccct tgtcccacac gatttctcct gtttctccgt 5941 ttgtttcgat aagtggtcgc ttccgaatct ctccattggc cagtgtaatc tcggtcttga 6001 aaaaattcat aatattgctg taaaagaagt acttagcggt ggccttgcct atttcctgct 6061 cagactttgc gatcattttc ctaacatcgt acactttata gtctccgtaa acaaattcag 6121 attcaagctt gggatatttt ttgataagtg cagtgcctac cactgcattc aggtaggcat 6181 catgcgcatg gtggtaattg ttgatctctc tcaccttata aaactgaaag tcctttctga 6241 aatctgagac cagcttagac ttcagagtaa taactttcac ctctcgaatc agtttgtcat 6301 tttcatcgta cttggtgttc atgcgtgaat cgagaatttg ggccacgtgc ttggtgatct 6361 ggcgtgtctc aacaagctgc cttttgatga agccggcttt atccaactca gacaggccac 6421 ctcgttcagc cttagtcaga ttatcgaact tccgttgtgt gatcagtttg gcgttcagca 6481 gctgccgcca ataatttttc attttcttga caacttcttc tgaggggacg ttatcactct 6541 tccctctatt tttatcggat cttgtcaaca ctttattatc aatagaatca tctttgagaa 6601 aagactgggg cacgatatga tccacgtcgt agtcggagag ccgattgatg tccagttcct 6661 gatccacgta catgtccctg ccgttctgca ggtagtacag gtagagcttc tcattctgaa 6721 gctgggtgtt ttcaactggg tgttccttaa ggatttggga ccccagttct tttataccct 6781 cttcaatcct cttcatcctt tccctactgt tcttctgtcc cttctgggta gtttggttct 6841 ctcgggccat ctcgataacg atattctcgg gcttatgcct tcccattact ttgacgagtt 6901 catccacgac cttaacggtc tgcagtattc cctttttgat agctgggcta cctgcaagat 6961 tagcgatgtg ctcgtgaaga ctgtccccct ggccagaaac ttgtgctttc tggatgtcct 7021 ccttaaaggt gagagagtca tcatggatca actgcatgaa gttccggttg gcaaatccat 7081 cggacttaag aaaatccagg attgtctttc cactctgctt gtctcggatc ccattgatca 7141 gttttcttga cagccgcccc catcctgtat atcggcgcct cttgagctgt ttcatgactt 7201 tgtcgtcgaa gagatgagcg taagttttca agcgttcttc aatcatctcc ctatcttcaa 7261 acaacgtaag ggtgaggaca atgtcctcaa gaatgtcctc gttctcctca ttgtccagga 7321 agtccttgtc tttaatgatt ttcaggagat cgtgatacgt tcccagggat gcgttgaagc 7381 gatcctccac tccgctgatt tcaacagagt cgaaacattc aatctttttg aaatagtctt 7441 ctttgagctg tttcacggta actttccggt tcgtcttgaa gaggaggtcc acgatagctt 7501 tcttctgctc tccagacagg aatgctggct ttctcatccc ttctgtgacg tatttgacct 7561 tggtgagctc gttataaact gtgaagtact cgtacagcag agagtgttta ggaagcacct 7621 tttcgttagg cagattttta tcaaagttag tcatcctttc gatgaaggac tgggcagagg 7681 cccccttatc cacgacttcc tcgaagttcc agggagtgat ggtctcttct gatttgcgag 7741 tcatccacgc gaatctggaa tttccccggg cgagggggcc tacatagtag ggtatccgaa 7801 atgtgaggat tttctcaatc ttttccctgt tatctttcaa aaaggggtag aaatcctctt 7861 gccgcctgag gatagcgtgc agttcgccca ggtgaatctg gtgggggatg cttccattgt 7921 cgaaagtgcg ctgtttgcgc aacagatctt ctctgttaag ctttaccagc agctcctcgg 7981 tgccgtccat tttttccaag atgggcttaa taaatttgta aaattcctcc tggcttgctc 8041 cgccgtcaat gtatccggcg tagccatttt tagactgatc gaagaaaatt tccttgtact 8101 tctcaggcag ttgctgtctg acaagggcct tcagcaaagt caagtcttgg tggtgctcat 8161 catagcgctt gatcatacta gcgctcagcg gagctttggt gatctccgtg ttcactcgca 8221 gaatatcact cagcagaatg gcgtctgaca ggttctttgc cgccaaaaaa aggtctgcgt 8281 actggtcgcc gatctgggcc agcagattgt cgagatcatc atcgtaggtg tctttgctca 8341 gttgaagctt ggcatcttcg gccaggtcga agttagattt aaagttgggg gtcagcccga 8401 gtgacagggc gataagatta ccaaacaggc cgttcttctt ctccccaggg agctgtgcga 8461 tgaggttttc gagccgccgg gatttggaca gcctagcgct caggattgct ttggcgtcaa 8521 ctccggatgc gttgatcggg ttctcttcga aaagctgatt gtaagtctga accagttgga 8581 taaagagttt gtcgacatcg ctgttgtctg ggttcaggtc cccctcgatg aggaagtgtc 8641 cccgaaattt gatcatatgc gccagcgcga gatagatcaa ccgcaagtca gccttatcag 8701 tactgtctac aagcttcttc ctcagatgat atatggttgg gtacttttca tggtacgcca 8761 cctcgtccac gatattgcca aagattgggt ggcgctcgtg ctttttatcc tcctccacca 8821 aaaaggactc ctccagccta tggaagaaag agtcatccac cttagccatc tcattactaa 8881 agatctcctg caggtagcag atccgattct ttctgcgggt atatctgcgc cgtgctgttc 8941 ttttgagccg cgtggcttcg gccgtctccc cggagtcgaa caggagggcg ccaatgaggt 9001 tcttctttat gctgtggcga tcggtattgc ccagaacttt gaattttttg ctcggcacct 9061 tgtactcgtc cgtaatgacg gcccagccga cgctgtttgt gccgatatcg agcccaatgg 9121 agtacttctt gtccaccttc cgctttttct tgggcatgct cgagggttgc gtgtgtatct 9181 gtgtgcagtg gatattgtta ccgagtttgg tgagcgtgag tccgttagtg ccctggtggt 9241 ggtggattag gagagtgggt gactcggtgt ccatggcttt cttcgctcat tataggaggg 9301 gaaaggaatg agggagggtg gggagaccgc gtctgttgtt gaccaccgat ttacttcttg 9361 cctcccttcc cctccctccc ctcaatccgt acgacacaaa tagtagccga gtgtctgctg 9421 cagagcgcat gattagtgtg gtagacaacg agggagggaa ggatgtacag ggcatggcac 9481 ggagaagcga tggtggccag gaagaggaga ggtcgcgaga acaggatgtg ttgcgaatgg 9541 ataaaaacag aaagcgatgg ctctgggctt cgaaagcagg ggacattagg acgtgtagac 9601 catctcgacg gatccctctg tatctctgtt gtgcgtgaat gttttctgtg cacgtgtagt 9661 gtgtgagagt agaacccggg aactcgaaca gagaaaagca tgggtggctg tggtgtggag 9721 gcttcgttcc caccacatgc ccttctcctt cgcctcgcct ctccctgcct tcttccacgc 9781 acccttgcgc ccctcgttct caatacctgg ctcacttcca ccattcaaac aaccatcacg 9841 atacaggcat ttatctatcg ttgaagactt cttcctccgg tagatcttag ccaaggtaag 9901 aagaggggca tgcagcaagg agaaagaaat gatgcatgat gaggaataga aggggaggag 9961 ggagggatat gatgggaagc gaaagcgcat attctggtgg tctgctgcct gatggggacg 10021 cgtctagctg tgacactgag gacggtggct gctggtggct gcgggcgctg ccttggtgat 10081 caatgggagt aaagggaggg aaggaggtag cgtgaacgga tgacgcggag aagtttaggg 10141 gtctctttac gtatcgcccc tgccgcccgc ctctctgcga taaatgtgcc tgttaccctg 10201 cagcctctat tcttcactgt gttcctgttt tccaacagcc tctattcttc cctgtctttt 10261 gttgcagtgg cgtcatcctc tctttgcccc agtcgtcgtt ctctcgactc actcactccc 10321 cccctccttc cctccctcca tccacagaat cgatgaagag caggccttgg ctcttcgttt 10381 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca 10441 ccgagtcggt gcttttggcc ggcatggtcc cagcctcctc gctggcgccg gctgggcaac 10501 atgcttcggc atggcgaatg ggactcctgg gtaccatggg aaagaaagga tgagaaagga 10561 gaaaggacat ctagataacc ggcatatcac ggtggtgtat tagtgtagaa tagtgaagag 10621 aagacttggg aaaatgtgta ggaaaggttg tttctgtgta tgtgggttgg gatgggtggc 10681 tgtttgagaa ggaacagcgg gcagggcgat gtagtgctga acgggcacgg aaccactgag 10741 actgaaggaa gtagggagag agaggggcag gggacgtgca ctttaatctt tgcctcggta 10801 gagtataccc atgcaagagt atgtggccac ctgtggtggc ttttggccag gtctggtgca 10861 gtgccaatca tctcccatca ataatacaac ttcagaacaa cggcgcattg atggggagag 10921 agaaagtaaa tttaagtaag gggtacgtag tagaggattc aactgaaata ttttcgagga 10981 gcggttggga agttgaccga ttgaaaggag aagggagggg agcaggtgtg atagtcatgt 11041 gtaaagtaat tcttttttgc cgtcgtcaca caatccacat caatgataaa atatgtttaa 11101 ggatcaatca cacggagtcg gtcataaggc aaccgcaaac gcaatgcaaa ctagcaagca 11161 agcaggacca caacaacaac cattaccatc acaggcgaca gcagcagcag ctgcagcagc 11221 agcaaggcaa gcaaaggcca tctgcgcgtg gacttttcca ggcaccggct ttaagcgtaa 11281 ttttcaatga ttgttgtccg tgtatcctct caccctttca ccgcttgcgc gcacgtgagc 11341 agcacaactg gtaatgcccg aggacaatag acgaggaaaa agaagaagaa aaatgaagag 11401 ggagtgatga agaagaagag aagaataaaa gaagacagaa aagaagataa aaaagtcaaa 11461 agacgacaaa gacggaaaaa aaagaaacgc gcaattttaa caccagcaac gacgacgaaa 11521 agggcgtcag ctttgc //