LOCUS       Exported                6766 bp ds-DNA     circular SYN 28-AUG-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pNOC-ARS-stacked-NeoR-aequorin.dna
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 8, 2017 from SnapGene Viewer 4.0.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6766)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      5..781)
                     /label=HS terminator
     CDS             complement(797..1588))
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        1601..2781)
                     /label=Ribi
     CDS             2796..3518)
                     /codon_start=1
                     /label=aequorin
                     /translation="MSGGSGSGGQSGGSGSGGQSGGSGSGGQSGGSGSGGQSGGSGSGG
                     QTSKYAVKLEPDFENPRWIGRHKHMFNFLDVNQNGKISLDEMVYKASDIVINNLGATPE
                     QAKRHKEAVEAFFGGAGMKYGVETEWPEYIKGWKKLAQTELDKFAKNQVTLIRLWGDAL
                     LDIIDKDQNGAITLDEWKKYTQSAGIIQSAEDCEETFKVCDLDDSGRLDADEMTRQHIG
                     FWYTMDPACEKLYGGAVP"
     terminator      3523..4406)
                     /label=LDSP terminator
     rep_origin      complement(4480..5068))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5239..6099))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6100..6204))
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    6241..6744)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 taatggatgg gaagtcggta agtgaaaagg gctttgtgta aggtggatgt caaattttgc
       61 caacccgtgc ctctgtgttc gcagtgtagc aaggaagccc acaccaccgc ctcatattca
      121 aatcgaaatc atcgaagaaa tcggcgagga tctcatgccc caaagctgtc atcaaatgtg
      181 tcccaccaca cgaagatgcc gagtaaatga cgtgagttgt cctatgattg gcaggatgtg
      241 gcatttaaag tccaaaaggc cctgcttggt cggtcacctt ttgctcgcct ccccctcccg
      301 tcacacccta aacacgaatg cagaagaaaa tcatgtgggg aagcaacgat ttagaaatgg
      361 gaatggaggc tcttcacttc ctgaatctga ccaagctggc ggacacaacc gtagagggat
      421 ctttgacctg catcaataca ccgacatgtt aataaatcga gtaccaaaga aaagctactg
      481 aagtcagttt cggattgtgt tgtagaatct tttgagtcgt cactggaatg ctatgctcgc
      541 ttcaatcgtc gattagcttt gtaaattatt tctggtggca aggcggggtt caagtagcaa
      601 gtgggggcat ggcagcaccc ctccgagcct cgttcgttca ggtgtttttg ggacctttcc
      661 ctcccttcct gtcttcagac gcttttattt tcctttccgt tttctactag tgacaaaaaa
      721 agggcacaca ctctttactc gctcacaccc tcgtactcgg acacgcgctg ttaccgactc
      781 tggtacctca agcgctgaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat
      841 cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt
      901 cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc
      961 cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat
     1021 cgccatgggt cacgacgaga tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca
     1081 gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg
     1141 cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg
     1201 tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg
     1261 caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt
     1321 cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca
     1381 gccacgatag ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac aggtcggtct
     1441 tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc
     1501 cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac
     1561 ctgcgtgcaa tccatcttgt tcaatcatgg gcccctcgag ggttgcgtgt gtatctgtgt
     1621 gcagtggata ttgttaccga gtttggtgag cgtgagtccg ttagtgccct ggtggtggtg
     1681 gattaggaga gtgggtgact cggtgtccat ggctttcttc gctcattata ggaggggaaa
     1741 ggaatgaggg agggtgggga gaccgcgtct gttgttgacc accgatttac ttcttgcctc
     1801 ccttcccctc cctcccctca atccgtacga cacaaatagt agccgagtgt ctgctgcaga
     1861 gcgcatgatt agtgtggtag acaacgaggg agggaaggat gtacagggca tggcacggag
     1921 aagcgatggt ggccaggaag aggagaggtc gtgagaacag gatgtgttgc gaatggataa
     1981 aaacagaaag cgatggctct gggcttcgaa agcaggggac attaggacgt gtagaccatc
     2041 tcgacggatc cctctgtatc tctgttgtgc gtgaatgttt tctgtgcacg tgtagtgtgt
     2101 gagagtagaa cccgggaact cgaacagaga aaagcatggg tggctgtggt gtggaggctt
     2161 cgttcccacc acatgccctt ctccttcgcc tcgcctctcc ctgccttctt ccacgcaccc
     2221 ttgcgcccct cgttctcaat acctggctca cttccaccat tcaaacaacc atcacgatac
     2281 aggcatttat ctatcgttga agacttcttc ctccggtaga tcttagccaa ggtaagaaga
     2341 ggggcatgca gcaaggagaa agaaatgatg catgatgagg aatagaaggg gaggagggag
     2401 ggatatgatg ggaagcgaaa gcgcatattc tggtggtctg ctgcctgatg gggagtctag
     2461 ctgtgacact gaggacggtg gctgctggtg gctgcgggcg ctgccttggt gatcaatggg
     2521 agtaaaggga gggaaggagg tagcgtgaac ggatgacgcg gagaagttta ggggtctctt
     2581 tacgtatcgc ccctgccgcc cgcctctctg cgataaatgt gcctgttacc ctgcagcctc
     2641 tattcttcac tgtgttcctg ttttccaaca gcctctattc ttccctgtct tttgttgcag
     2701 tggcgtcatc ctctctttgc cccagtcgtc gttctctcga ctcactcact ccccccctcc
     2761 ttccctccct ccatccacag aatcgattgg cgcgtatgtc gggcggtagc ggaagtgggg
     2821 gccagagcgg ggggagtggc tccggaggac agtccggcgg gagcgggtca ggcggtcaat
     2881 ccggcggtag cgggtcgggg gggcaatcgg gaggctccgg ttctgggggc cagacgagca
     2941 agtacgccgt gaaattggag cccgatttcg agaaccctcg atggatcggg cgtcataagc
     3001 acatgtttaa cttcttggac gtgaatcaaa atggtaagat tagcttagac gagatggttt
     3061 acaaggcaag tgacattgtt attaacaatc taggcgcaac cccggagcaa gcgaagcgtc
     3121 acaaggaagc ggtggaggcg ttcttcggcg gagctggcat gaagtacggg gtggagaccg
     3181 agtggcccga gtacatcaag gggtggaaga agttggccca aactgaactg gacaagttcg
     3241 ccaaaaacca ggtcacactc atacggctct ggggggacgc gctgcttgat attattgaca
     3301 aggatcagaa tggcgctatt accctggacg agtggaagaa gtacactcaa tcggctggga
     3361 ttatccagag cgccgaggat tgcgaggaga cctttaaggt ctgcgatctt gacgattccg
     3421 ggcgtttgga tgcagacgaa atgacccgac agcatatcgg tttttggtac acgatggacc
     3481 ccgcctgtga gaagctctac ggcggcgcgg tcccttgagc tcgaaagatc caagagagac
     3541 gagtagagat tttttttttg ggattgatgt ttgtcgttct ttgagttgtc gtcgagttac
     3601 gccttttgta agaatgttcc gcaggagagg aggaggatgg gcatgagtga gggtgagagg
     3661 gcttgcccgc tttttttttt aaaaacgctg ctcttcgtgg ttgtcaaaca aaccccccat
     3721 agaaacgatt ttgttacggt gcggtccaga cgtcacttga atggctccgc ggaaaggcca
     3781 gggagggaag gggggaggga ggaaacatga aacatgttga acggctcaac agggtttggg
     3841 ggacaagaga ggtagcgccc tgatggactg ctccctcccc tcctttccct caatgtctca
     3901 ttcatccatg cttccccctt ctctctctcc cctccgttcc atcccccgcg ggcgtggtag
     3961 tggcgtgatg ggatccacta aaatgtacgt gtaagaaaag ccggtgagct tacgcttttg
     4021 tgaaagtggg agtacgagtg ttgtgtgtgt gtgtagtggt ttcagacccc agacagaggc
     4081 gaagcagaaa aagcagacga tgaagacgac gaagaaatga gcagtctatt tttatcgtgg
     4141 aaacagaaga ggtgatatcg tctcgttctt tgttatcacc taccccgcgt gcatgtacat
     4201 gcagcctttt tattttgtaa tctttcccga aaaatcaacc gccacctccc ccccgccttc
     4261 tctcacccat catcttctcc tgtttatctt ctactttaca ctagatcgca tggcacatct
     4321 ccctcgcaat ccatcggtgc aaccatcatc gatcccactc ctccctccct ccctccctcc
     4381 ctcctcgcat ctcttctaag aaatccgcta gtgtttaaac atgtgagcaa aaggccagca
     4441 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4501 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4561 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4621 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4681 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4741 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4801 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4861 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4921 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4981 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     5041 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     5101 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5161 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5221 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5281 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5341 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5401 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5461 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5521 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     5581 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5641 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5701 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5761 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     5821 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     5881 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     5941 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6001 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6061 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     6121 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     6181 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggtccttttc
     6241 atcacgtgct ataaaaataa ttataattta aattttttaa tataaatata taaattaaaa
     6301 atagaaagta aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa gatgtaaaag
     6361 actctagggg gatcgccaac aaatactacc ttttatcttg tacttcctgc tctcaggtat
     6421 taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac acacgaaaat cctgtgattt
     6481 tacattttac ttatcgttaa tcgaatgtat atctatttaa tctgcttttc ttgtctaata
     6541 aatatatatg taaagtacgc tttttgttga aattttttaa acctttgttt attttttttt
     6601 cttcattccg taactcttct accttcttta tttactttct aaaatccaaa tacaaaacat
     6661 aaaaataaat aaacacagag taaattccca aattattcca tcattaaaag atacgaggcg
     6721 cgtgtaagtt acaggcaagc gatccgtcta agaaaccggt taaggc
//
