LOCUS       Exported                9042 bp ds-DNA     circular SYN 09-AUG-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 7, 2018 from SnapGene 4.1.9
            http://www.snapgene.com
COMMENT     This is pRK5 carrying the rat GluR2Q flip coding region. This has 
            been sequenced from the pMB1 ori to the SV40 polyA  (except the 45 
            bp in magenta at the C-terminus) and is correct.
            The vector sequence is from 
            http://ebv.oncology.wisc.edu/plasmids/J-S/pRK5.html
            on this site Bill Sugden at Univ. of Wisc. states ""Note: This 
            plasmid has not been sequenced in it's entirety. The sequence in 
            this file was assembled using sequences from GenBank and many not be
            completely accurate.""
            I identified the regions by comparing them to other sequences off 
            the web. See individual regions for details.
            
            M85035. Rat glutamate rec...[gi:204381]
                                                                                
            Related Sequences, Protein, PubMed, Taxonomy, LinkOut
            
            LOCUS       RATGLUR2A               3488 bp    mRNA    linear   ROD 
            27-APR-1993
            DEFINITION  Rat glutamate receptor subunit 2 (GLUR2) non-NMDA mRNA, 
            complete
                        cds.
            ACCESSION   M85035
            VERSION     M85035.1  GI:204381
            KEYWORDS    glutamate receptor subunit 2.
            SOURCE      Rattus norvegicus (strain Sprague-Dawley) (library: 
            lambda ZAP II)
                        adult forebrain cDNA to mRNA.
              ORGANISM  Rattus norvegicus
                        Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; 
            Euteleostomi;
                        Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; 
            Murinae;
                        Rattus.
            REFERENCE   1  (bases 1 to 3488)
              AUTHORS   Boulter,J., Hollmann,M., O'Shea-Greenfield,A., 
            Hartley,M.,
                        Deneris,E., Maron,C. and Heinemann,S.
              TITLE     Molecular cloning and functional expression of Glutamate
            receptor
                        subunit genes
              JOURNAL   Science 249, 1033-1037 (1990)
              MEDLINE   90371298
            COMMENT     original published sequence name: RATGLUR2.
            FEATURES             Location/Qualifiers
                 source          1..3488
                                 /organism=""Rattus norvegicus""
                                 /strain=""Sprague-Dawley""
                                 /db_xref=""taxon:10116""
                                 /tissue_type=""forebrain""
                                 /dev_stage=""adult""
                                 /tissue_lib=""lambda ZAP II""
                 misc_feature    1..14
                                 /gene=""GLUR2""
                                 /note=""EcoRI site and adaptor from Lambda 
            Zap""
                 gene            1..3488
                                 /gene=""GLUR2""
                 mRNA            15..3488
                                 /gene=""GLUR2""
                 CDS             316..2967
                                 /gene=""GLUR2""
                                 /codon_start=1
                                 /product=""glutamate receptor subunit 2""
                                 /protein_id=""AAA41240.1""
                                 /db_xref=""GI:204382""
                                 
            /translation=""MQKIMHISVLLSPVLWGLIFGVSSNSIQIGGLFPRGADQEYSAF
                                 
            RVGMVQFSTSEFRLTPHIDNLEVANSFAVTNAFCSQFSRGVYAIFGFYDKKSVNTITS
                                 
            FCGTLHVSFITPSFPTDGTHPFVIQMRPDLKGALLSLIEYYQWDKFAYLYDSDRGLST
                                 
            LQAVLDSAAEKKWQVTAINVGNINNDKKDETYRSLFQDLELKKERRVILDCERDKVND
                                 
            IVDQVITIGKHVKGYHYIIANLGFTDGDLLKIQFGGANVSGFQIVDYDDSLVSKFIER
                                 
            WSTLEEKEYPGAHTATIKYTSALTYDAVQVMTEAFRNLRKQRIEISRRGNAGDCLANP
                                 
            AVPWGQGVEIERALKQVQVEGLSGNIKFDQNGKRINYTINIMELKTNGPRKIGYWSEV
                                 
            DKMVVTLTELPSGNDTSGLENKTVVVTTILESPYVMMKKNHEMLEGNERYEGYCVDLA
                                 
            AEIAKHCGFKYKLTIVGDGKYGARDADTKIWNGMVGELVYGKADIAIAPLTITLVREE
                                 
            VIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSR
                                 
            FSPYEWHTEEFEDGRETQSSESTNEFGIFNSLWFSLGAFMRQGCDISPRSLSGRIVGG
                                 
            VWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLSKQTEIAYGTLDSGSTKEFFRR
                                 
            SKIAVFDKMWTYMRSAEPSVFVRTTAEGVARVRKSKGKYAYLLESTMNEYIEQRKPCD
                                 
            TMKVGGNLDSKGYGIATPKGSSLGNAVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGG
                                 
            GDSKEKTSALSLSNVAGVFYILVGGLGLAMLVALIEFCYKSRAEAKRMKVAKNPQNIN
                                 PSSSQNSQNFATYKEGYNVYGIESVKI""
                 sig_peptide     316..378
                                 /gene=""GLUR2""
                 mat_peptide     379..2964
                                 /gene=""GLUR2""
                                 /product=""glutamate receptor subunit 2""
                 polyA_site      3488
                                 /gene=""GLUR2""
            BASE COUNT     1015 a    696 c    869 g    908 t
FEATURES             Location/Qualifiers
     source          1..9042
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    90..615
                     /label=CMV promoter
     misc_feature    828..845
                     /label=SP6
     misc_feature    2101..2119
                     /label=Tn5 ME
                     /note="Epicentre Tn5 ME"
     misc_feature    2122..2851
                     /label=the perfect transposon (eGFP)
                     /note="Made by subcloning PCR fragment from pUniVHis-topo 
                     amplified with primers 63934 and 63935 into pCRBlunt-topo.
                     pBNJ10.13, pBNJ10.14 in Doug's Box 2 (-20 C)
                     Pg. 115.1, 116.1, 118.1  "
     misc_feature    2856..2874
                     /label=perfect transposon
                     /note="Made by subcloning PCR fragment from pUniVHis-topo 
                     amplified with primers 63934 and 63935 into pCRBlunt-topo.
                     pBNJ10.13, pBNJ10.14 in Doug's Box 2 (-20 C)
                     Pg. 115.1, 116.1, 118.1  "
     misc_feature    complement(2856..2874)
                     /label=Tn5 ME
                     /note="Epicentre Tn5 ME"
     misc_feature    2875..2883
                     /label=9bp repeat
     misc_feature    2875..2883
                     /label=pGluR2Qi
                     /note="This is pRK5 carrying the rat GluR2Q flip coding 
                     region. This has been sequenced from the pMB1 ori to the 
                     SV40 polyA  (except the 45 bp in magenta at the C-terminus)
                     and is correct.
                     The vector sequence is from 
                     http://ebv.oncology.wisc.edu/plasmids/J-S/pRK5.html
                     on this site Bill Sugden at Univ. of Wisc. states ""Note: 
                     This plasmid has not been sequenced in it's entirety. The 
                     sequence in this file was assembled using sequences from 
                     GenBank and many not be
                     completely accurate.""
                     I identified the regions by comparing them to other 
                     sequences off the web. See individual regions for details.
                     
                     M85035. Rat glutamate rec...[gi:204381]
                                                                                
                              Related Sequences, Protein, PubMed, Taxonomy, 
                     LinkOut
                     
                     LOCUS       RATGLUR2A               3488 bp    mRNA    
                     linear   ROD 27-APR-1993
                     DEFINITION  Rat glutamate receptor subunit 2 (GLUR2) 
                     non-NMDA mRNA, complete
                                 cds.
                     ACCESSION   M85035
                     VERSION     M85035.1  GI:204381
                     KEYWORDS    glutamate receptor subunit 2.
                     SOURCE      Rattus norvegicus (strain Sprague-Dawley) 
                     (library: lambda ZAP II)
                                 adult forebrain cDNA to mRNA.
                       ORGANISM  Rattus norvegicus
                                 Eukaryota; Metazoa; Chordata; Craniata; 
                     Vertebrata; Euteleostomi;
                                 Mammalia; Eutheria; Rodentia; Sciurognathi; 
                     Muridae; Murinae;
                                 Rattus.
                     REFERENCE   1  (bases 1 to 3488)
                       AUTHORS   Boulter,J., Hollmann,M., O'Shea-Greenfield,A., 
                     Hartley,M.,
                                 Deneris,E., Maron,C. and Heinemann,S.
                       TITLE     Molecular cloning and functional expression of 
                     Glutamate receptor
                                 subunit genes
                       JOURNAL   Science 249, 1033-1037 (1990)
                       MEDLINE   90371298
                     COMMENT     original published sequence name: RATGLUR2.
                     FEATURES             Location/Qualifiers
                          source          1..3488
                                          /organism=""Rattus norvegicus""
                                          /strain=""Sprague-Dawley""
                                          /db_xref=""taxon:10116""
                                          /tissue_type=""forebrain""
                                          /dev_stage=""adult""
                                          /tissue_lib=""lambda ZAP II""
                          misc_feature    1..14
                                          /gene=""GLUR2""
                                          /note=""EcoRI site and adaptor from 
                     Lambda Zap""
                          gene            1..3488
                                          /gene=""GLUR2""
                          mRNA            15..3488
                                          /gene=""GLUR2""
                          CDS             316..2967
                                          /gene=""GLUR2""
                                          /codon_start=1
                                          /product=""glutamate receptor subunit 
                     2""
                                          /protein_id=""AAA41240.1""
                                          /db_xref=""GI:204382""
                                          
                     /translation=""MQKIMHISVLLSPVLWGLIFGVSSNSIQIGGLFPRGADQEYSAF
                                          
                     RVGMVQFSTSEFRLTPHIDNLEVANSFAVTNAFCSQFSRGVYAIFGFYDKKSVNTITS
                                          
                     FCGTLHVSFITPSFPTDGTHPFVIQMRPDLKGALLSLIEYYQWDKFAYLYDSDRGLST
                                          
                     LQAVLDSAAEKKWQVTAINVGNINNDKKDETYRSLFQDLELKKERRVILDCERDKVND
                                          
                     IVDQVITIGKHVKGYHYIIANLGFTDGDLLKIQFGGANVSGFQIVDYDDSLVSKFIER
                                          
                     WSTLEEKEYPGAHTATIKYTSALTYDAVQVMTEAFRNLRKQRIEISRRGNAGDCLANP
                                          
                     AVPWGQGVEIERALKQVQVEGLSGNIKFDQNGKRINYTINIMELKTNGPRKIGYWSEV
                                          
                     DKMVVTLTELPSGNDTSGLENKTVVVTTILESPYVMMKKNHEMLEGNERYEGYCVDLA
                                          
                     AEIAKHCGFKYKLTIVGDGKYGARDADTKIWNGMVGELVYGKADIAIAPLTITLVREE
                                          
                     VIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSR
                                          
                     FSPYEWHTEEFEDGRETQSSESTNEFGIFNSLWFSLGAFMRQGCDISPRSLSGRIVGG
                                          
                     VWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLSKQTEIAYGTLDSGSTKEFFRR
                                          
                     SKIAVFDKMWTYMRSAEPSVFVRTTAEGVARVRKSKGKYAYLLESTMNEYIEQRKPCD
                                          
                     TMKVGGNLDSKGYGIATPKGSSLGNAVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGG
                                          
                     GDSKEKTSALSLSNVAGVFYILVGGLGLAMLVALIEFCYKSRAEAKRMKVAKNPQNIN
                                          PSSSQNSQNFATYKEGYNVYGIESVKI""
                          sig_peptide     316..378
                                          /gene=""GLUR2""
                          mat_peptide     379..2964
                                          /gene=""GLUR2""
                                          /product=""glutamate receptor subunit 
                     2""
                          polyA_site      3488
                                          /gene=""GLUR2""
                     BASE COUNT     1015 a    696 c    869 g    908 t"
     misc_feature    3812..3814
                     /label=R606Q
     misc_feature    5355..5476
                     /label=SV40 poly A
                     /note="this sequence matches the last 121bp of the SV40 
                     region as defined by
                     http://www.coloncancer.org/adeasy.htm"
     misc_feature    complement(5452..5476)
                     /label=64493
     misc_feature    complement(6164..6432)
                     /label=f1 (-)
                     /note="f1 intergenic origin region defined by sequence from
                     http://www.fermentas.com/techinfo/NucleicAcids/mapptz19ru.h
                     tm
                     blue region is between the two origins (-) points toward 0,
                     (+) points toward 4661
                     red thymidine is an adenosine in their sequence"
     misc_feature    6458..6619
                     /label=f1 (+)
                     /note="f1 intergenic origin region defined by sequence from
                     http://www.fermentas.com/techinfo/NucleicAcids/mapptz19ru.h
                     tm
                     blue region is between the two origins (-) points toward 0,
                     (+) points toward 4661
                     red thymidine is an adenosine in their sequence"
     CDS             7006..7866
                     /codon_start=1
                     /label=Amp
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    8011..8685
                     /label=pMB1 ori
                     /note="sequence match to better pGreen lantern map.
                     the single red thymidine is a cytosine in the other 
                     sequence
                     the two adjacent bases in red are inverted in the sequence 
                     from pGreen lantern"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attccccggg gatcctctag aactagtgga tcccccgggc
      961 tgcaggaatt cggcacgagg ccagtcctcg ggacttcggg agcagggaca ggacgcaagg
     1021 catcaacagc caccagctac aactgggaaa taaggattct tctgccttca cttcgtgttt
     1081 ttagcagctc cttgctaaat atcgacctca caatgcagag gatctaattt gctgaggaaa
     1141 acagtcaaag aaggaagagg aagaaaggga aacgagggga tattttgtgg atgctctact
     1201 tttcttggaa atgcaaaaga ttatgcatat ttctgtcctc ctttctcctg ttttatgggg
     1261 actgattttt ggtgtctctt ctaacagcat acagataggg gggctatttc caaggggcgc
     1321 tgatcaagaa tacagtgcat ttcgggtagg gatggttcag ttttccactt cggagttcag
     1381 actgacaccc catatcgaca atttggaggt agccaacagt ttcgcagtca ccaatgcttt
     1441 ctgctcccag ttttcaagag gagtctacgc aatttttgga ttttatgaca agaagtctgt
     1501 aaataccatc acatcattct gtgggacact ccatgtgtcc ttcatcacac ctagcttccc
     1561 aacagatggc acacatccat ttgtcatcca gatgcgacct gacctcaaag gagcactcct
     1621 tagcttgatt gagtactacc aatgggacaa gttcgcatac ctctatgaca gtgacagagg
     1681 cttatcaaca ctgcaagctg ttctggattc tgctgcagag aagaagtggc aggtgactgc
     1741 tatcaatgtg gggaacatca acaatgacaa gaaagatgag acctacagat cgctctttca
     1801 agatctggag ttaaaaaaag aacggcgtgt aatcctggac tgtgaaaggg ataaagtaaa
     1861 tgacattgtg gaccaggtta ttaccattgg aaaacatgtt aaagggtacc attatatcat
     1921 tgcaaatctg ggattcactg atggggacct gctgaaaatt cagtttggag gagcaaatgt
     1981 ctctggattt cagattgtag actacgatga ttccctggtg tctaaattta tagaaagatg
     2041 gtcaacactg gaagagaaag aataccctgg agcacacaca gcgacaatta agtatacttc
     2101 ctgtctctta tacacatctg gcgcgccgag caagggcgag gagctgttca ccggggtggt
     2161 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     2221 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     2281 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag
     2341 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     2401 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     2461 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     2521 ggacggcaac atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat
     2581 caccgccgac aagcagaaga acggcatcaa ggcccacttc aagatccgcc acaacatcga
     2641 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     2701 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     2761 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     2821 catggacgag ctgtacaagg gcggcgcgcc cgggcagatg tgtataagag acaggtatac
     2881 ttcggccctg acgtatgatg ctgtccaagt gatgactgaa gcattccgta accttcggaa
     2941 gcagaggatt gaaatatccc ggagaggaaa tgcaggggat tgtttggcca acccagctgt
     3001 gccctgggga caaggggtcg aaatagaaag ggccctcaag caggttcaag ttgaaggcct
     3061 ctctggaaat ataaagtttg accagaatgg aaaacgaata aactacacaa ttaacatcat
     3121 ggagctcaaa acaaatggac cccggaagat tgggtactgg agtgaagtgg ataaaatggt
     3181 tgtcacccta actgagctcc catcaggaaa tgacacgtct gggcttgaaa acaagactgt
     3241 ggtggtcacc acaatattgg aatctccata tgttatgatg aagaaaaatc atgaaatgct
     3301 tgaagggaat gagcgttacg agggctactg tgttgactta gctgcagaaa ttgccaaaca
     3361 ctgtgggttc aagtacaagc tgactattgt tggggatggc aagtatgggg ccagggatgc
     3421 cgacaccaaa atttggaatg gtatggttgg agagcttgtc tacgggaaag ctgacattgc
     3481 aattgctcca ttaactatca ctctcgtgag agaagaggtg attgacttct ccaagccctt
     3541 catgagtctt ggaatctcta tcatgatcaa gaagcctcag aagtccaaac caggagtgtt
     3601 ttcctttctt gatcctttag cctatgagat ctggatgtgc attgtgtttg cctacattgg
     3661 ggtcagtgta gttttattcc tggtcagcag atttagcccc tacgagtggc acactgagga
     3721 atttgaagat ggaagagaaa cacaaagtag tgaatcaact aatgaatttg ggatttttaa
     3781 tagtctctgg ttttccttgg gtgcctttat gcagcaggga tgcgatattt cgccaagatc
     3841 cctctctggg cgcattgttg gaggtgtgtg gtggttcttt accctgatca taatctcctc
     3901 ctacacggct aacttagctg ccttcctgac tgtagagagg atggtgtctc ccatcgaaag
     3961 tgctgaggat ctgtctaagc aaacagaaat tgcttatgga acattagact ctggctccac
     4021 taaagagttt ttcaggagat ctaaaatcgc agtgtttgat aaaatgtgga cttatatgag
     4081 gagtgcagag ccctctgtgt ttgtgaggac taccgcagaa ggagtagcca gagtccggaa
     4141 atccaaagga aagtatgcct acttgctgga gtccacaatg aacgagtaca tcgagcagag
     4201 gaagccttgt gacaccatga aagtgggagg aaacttggat tccaaaggct acggcatcgc
     4261 cacacctaaa ggatcctcat taggaacccc agtaaatctt gcagtattga aactcagtga
     4321 gcaaggcgtc ttagacaagc tgaaaaacaa atggtggtac gataaaggtg aatgtggagc
     4381 caaggactcg ggaagtaagg aaaagaccag tgccctcagt ctgagcaacg ttgctggagt
     4441 attctacatc cttgtcgggg gccttggttt ggcaatgctg gtggctttga ttgagttctg
     4501 ttacaagtca agggccgagg cgaaacgaat gaaggtggca aagaatccac agaatattaa
     4561 cccatcttcc tcgcagaatt cccagaattt tgcaacttat aaggaaggtt acaacgtata
     4621 tggcatcgag agtgttaaaa tttaggggat gaccttgagt gatgtcatga ggagcaaggc
     4681 aaggctgtca attacaggaa gtactggaga aaatggacgt gttatgactc cagaatttcc
     4741 caaagcagtg catgctgtcc cttacgtgag tcctggcatg ggaatgaatg tcagtgtgac
     4801 tgatctctcg tgattgatag gaaccttctg agtgccttac acaatggttt ccttgtgtgt
     4861 ttattgtcaa agtggtgaga ggcatccgat atcttgaagg cttttctttc agccaagaat
     4921 tcttaactat gtggagttca ccttgaattg taaggaaaga taaattacaa acagagcatc
     4981 attttctact ccgatatcag aggaagcgtg gtggacatgc acagctaaca tggaaatact
     5041 atcatttaac tgaagtcttt gtacagacaa caaacccgtt tccgcagcca ctattgttag
     5101 tctcttgatt cataatgact taagcacact tgacatcaac tgcatcaaga tgtgacctgt
     5161 tttataaata aaaaggaaaa aaacatttaa aataaaaaaa tatttttagg tattttcaca
     5221 gacaaactgg cttttaaata aatttgcttc cgtactggtt ggttaagaca aatacaaaaa
     5281 caaaaacaaa acaaaacaaa aaccaaaaaa aaaaaaaaaa aactcgacct gcagaagctt
     5341 ggccgccatg gcccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5401 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     5461 tcatcaatgt atcttatcat gtctggatcg ggaattaatt cggcgcagca ccatggcctg
     5521 aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag aaccagctgt
     5581 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5641 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     5701 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     5761 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     5821 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     5881 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct gttaacagct tggcactggc
     5941 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     6001 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     6061 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     6121 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     6181 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     6241 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     6301 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     6361 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     6421 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     6481 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     6541 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     6601 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     6661 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     6721 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     6781 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     6841 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     6901 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     6961 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     7021 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     7081 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     7141 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     7201 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     7261 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     7321 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     7381 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     7441 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     7501 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     7561 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     7621 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     7681 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     7741 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     7801 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     7861 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     7921 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     7981 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     8041 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     8101 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     8161 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     8221 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     8281 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     8341 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8401 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8461 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     8521 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     8581 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8641 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8701 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     8761 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     8821 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     8881 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     8941 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     9001 caatttcaca caggaaacag ctatgacatg attacgaatt aa
//
