LOCUS Exported 5716 bp ds-DNA circular SYN 11-AUG-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pAAV_hsyn1_NES-his-CaMPARI2-F391W-WPRE-SV40 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5716) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 11, 2017 from SnapGene 4.0.2 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5716 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 57..223 /label=ITR promoter 246..717 /label=synapsin promoter misc_feature 748..804 /note="NES" misc_feature 811..834 /note="His" CDS 835..2052 /codon_start=1 /note="CaMPARI" /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVVMMTAKLECEKIYVRDGVLTGD IHMILLLEGNAHYRCDFRTTYKAKEKGVKLPGVHYVDHTIEILSHDKDYNKVKIYEYAV AHSGLPDNARRGGTGGSMVSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMD LEVKEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSMTFEDGGI CYARSDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWMPSWTRSSRRKWNKTGHALR AIGRLSS" CDS 835..1275 /note="CaM" misc_feature 1258..1260 /label=Q142V CDS 1282..1533 /note="mEos - Cterm" misc_feature 1426..1428 /label=F198Y misc_feature 1438..1440 /label=C202T misc_feature 1483..1485 /label=L217I intron 1534..1551 /label=linker /note="linker" CDS 1552..1983 /note="mEos - Nterm" misc_feature 1738..1746 /note="Chromophore" misc_feature 1867..1869 /label=N345S CDS 1990..2052 /note="M13" variation 2005..2007 /label=F391W CDS 2071..2094 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 2113..2139 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 2158..2187 /codon_start=1 /product="Myc (human c-Myc oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" misc_feature 2209..2797 /label=WPRE misc_feature 2831..3062 /label=SV40 polyadenylation signal misc_feature complement(3091..3257) /label=ITR misc_feature 3916..4773 /label=Amp ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgcg 61 cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg 121 cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg 181 ttccttgtag ttaatgatta acccgccatg ctacttatct acgtagccat gctctaggaa 241 gatctctgca gagggccctg cgtatgagtg caagtgggtt ttaggaccag gatgaggcgg 301 ggtgggggtg cctacctgac gaccgacccc gacccactgg acaagcaccc aacccccatt 361 ccccaaattg cgcatcccct atcagagagg gggaggggaa acaggatgcg gcgaggcgcg 421 tgcgcactgc cagcttcagc accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc 481 caccgccgcc tcagcactga aggcgcgctg acgtcactcg ccggtccccc gcaaactccc 541 cttcccggcc accttggtcg cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg 601 aggcgcgaga taggggggca cgggcgcgac catctgcgct gcggcgccgg cgactcagcg 661 ctgcctcagt ctgcggtggg cagcggagga gtcgtgtcgt gcctgagagc gcagtcgaat 721 tcaagctgct agcaaggatc cgccaccatg ctgcagaacg agcttgctct taagttggct 781 ggacttgata ttaacaagac tggaggttct catcatcacc accaccatgg atccgaccaa 841 ctgactgaag agcagatcgc agaatttaaa gaggctttct ccctatttga caaggacggg 901 gatgggacaa taacaaccaa ggagctgggg acggtgatgc ggtctctggg gcagaacccc 961 acagaagcag agctgcagga catgatcaat gaagtagatg ccgacggtga cggcacaatc 1021 gacttccctg agttcctgac aatgatggca agaaaaatga aagacacaga cagtgaagaa 1081 gaaattagag aagcgttccg tgtgtttgat aaggatggca atggctacat cagtgcagca 1141 gagcttcgcc acgtgatgac aaaccttgga gagaagttaa cagatgaaga ggttgatgaa 1201 atgatcaggg aagcagacat cgatggggat ggtcaggtaa actacgaaga gtttgtagtg 1261 atgatgacag cgaagctcga gtgcgagaaa atctatgtgc gtgatggagt gctgacgggt 1321 gatattcata tgatcttgtt gcttgaagga aatgcccatt accgatgtga cttcagaact 1381 acttacaaag ctaaggagaa gggtgtcaag ttaccaggcg tgcactatgt ggaccacacc 1441 attgagattt taagccatga caaagattac aacaaggtta agatctatga gtatgctgtt 1501 gctcattctg gattgcctga caatgccaga cgaggcggta ccggcggatc catggtgagt 1561 gcgattaagc cagacatgaa gatcaaactg cgtatggaag gcaacgtaaa cgggcaccac 1621 tttgtgatcg acggagatgg tacaggcaag ccttatgagg gaaaacagac catggatctt 1681 gaagtcaaag agggcggacc tctgcctttt gcctttgata tcctgaccac tgcattccat 1741 tacggcaaca gggtattcgt gaaatatcca gacaacatac aagactattt taagcagtcg 1801 tttcctaagg ggtattcgtg ggaacgaagc atgactttcg aagacggggg catttgctat 1861 gccagaagcg acataacaat ggaaggggac actttctata ataaagttcg attttatggt 1921 accaactttc ccgccaatgg tccagttatg cagaagaaga cgctgaaatg gatgccgagc 1981 tggacgcgtt catcacgtcg taagtggaat aagacaggtc acgcactgag agctataggt 2041 cggctgagct caggaggttc tggaggttct gattacaagg acgacgatga caagggaggt 2101 tctggaggtt cttacccata cgacgtacca gattacgctg gaggttctgg aggttctgag 2161 cagaagctga tctcagagga ggacctgcgt acgtaaaagc ttatcgataa tcaacctctg 2221 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 2281 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 2341 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 2401 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 2461 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 2521 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 2581 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc 2641 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 2701 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct 2761 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgaccc 2821 gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact 2881 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta 2941 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag 3001 gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa 3061 atcgataagg atcttcctag agcatggcta cgtagataag tagcatggcg ggttaatcat 3121 taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct 3181 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt 3241 gagcgagcga gcgcgcagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 3301 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc 3361 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 3421 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 3481 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 3541 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 3601 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 3661 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 3721 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac 3781 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 3841 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 3901 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 3961 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 4021 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 4081 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 4141 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 4201 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 4261 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 4321 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 4381 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 4441 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 4501 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 4561 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 4621 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 4681 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 4741 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 4801 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 4861 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 4921 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 4981 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 5041 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 5101 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 5161 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 5221 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 5281 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 5341 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 5401 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 5461 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 5521 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 5581 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 5641 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 5701 agcgaggaag cggaag //