LOCUS       Exported                5716 bp ds-DNA     circular SYN 11-AUG-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV_hsyn1_NES-his-CaMPARI2-H396K-WPRE-SV40
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 11, 2017 from SnapGene 4.0.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    57..223
                     /label=ITR
     promoter        246..717
                     /label=synapsin promoter
     misc_feature    748..804
                     /note="NES"
     misc_feature    811..834
                     /note="His"
     CDS             835..2052
                     /codon_start=1
                     /note="CaMPARI"
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVVMMTAKLECEKIYVRDGVLTGD
                     IHMILLLEGNAHYRCDFRTTYKAKEKGVKLPGVHYVDHTIEILSHDKDYNKVKIYEYAV
                     AHSGLPDNARRGGTGGSMVSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMD
                     LEVKEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSMTFEDGGI
                     CYARSDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWMPSWTRSSRRKFNKTGKALR
                     AIGRLSS"
     CDS             835..1275
                     /note="CaM"
     misc_feature    1258..1260
                     /label=Q142V
     CDS             1282..1533
                     /note="mEos - Cterm"
     misc_feature    1426..1428
                     /label=F198Y
     misc_feature    1438..1440
                     /label=C202T
     misc_feature    1483..1485
                     /label=L217I
     intron          1534..1551
                     /label=linker
                     /note="linker"
     CDS             1552..1983
                     /note="mEos - Nterm"
     misc_feature    1738..1746
                     /note="Chromophore"
     misc_feature    1867..1869
                     /label=N345S
     CDS             1990..2052
                     /note="M13"
     variation       2020..2022
                     /label=H396K
     CDS             2071..2094
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2113..2139
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2158..2187
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    2209..2797
                     /label=WPRE
     misc_feature    2831..3062
                     /label=SV40 polyadenylation signal
     misc_feature    complement(3091..3257)
                     /label=ITR
     misc_feature    3916..4773
                     /label=Amp
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgcg
       61 cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg
      121 cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg
      181 ttccttgtag ttaatgatta acccgccatg ctacttatct acgtagccat gctctaggaa
      241 gatctctgca gagggccctg cgtatgagtg caagtgggtt ttaggaccag gatgaggcgg
      301 ggtgggggtg cctacctgac gaccgacccc gacccactgg acaagcaccc aacccccatt
      361 ccccaaattg cgcatcccct atcagagagg gggaggggaa acaggatgcg gcgaggcgcg
      421 tgcgcactgc cagcttcagc accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc
      481 caccgccgcc tcagcactga aggcgcgctg acgtcactcg ccggtccccc gcaaactccc
      541 cttcccggcc accttggtcg cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg
      601 aggcgcgaga taggggggca cgggcgcgac catctgcgct gcggcgccgg cgactcagcg
      661 ctgcctcagt ctgcggtggg cagcggagga gtcgtgtcgt gcctgagagc gcagtcgaat
      721 tcaagctgct agcaaggatc cgccaccatg ctgcagaacg agcttgctct taagttggct
      781 ggacttgata ttaacaagac tggaggttct catcatcacc accaccatgg atccgaccaa
      841 ctgactgaag agcagatcgc agaatttaaa gaggctttct ccctatttga caaggacggg
      901 gatgggacaa taacaaccaa ggagctgggg acggtgatgc ggtctctggg gcagaacccc
      961 acagaagcag agctgcagga catgatcaat gaagtagatg ccgacggtga cggcacaatc
     1021 gacttccctg agttcctgac aatgatggca agaaaaatga aagacacaga cagtgaagaa
     1081 gaaattagag aagcgttccg tgtgtttgat aaggatggca atggctacat cagtgcagca
     1141 gagcttcgcc acgtgatgac aaaccttgga gagaagttaa cagatgaaga ggttgatgaa
     1201 atgatcaggg aagcagacat cgatggggat ggtcaggtaa actacgaaga gtttgtagtg
     1261 atgatgacag cgaagctcga gtgcgagaaa atctatgtgc gtgatggagt gctgacgggt
     1321 gatattcata tgatcttgtt gcttgaagga aatgcccatt accgatgtga cttcagaact
     1381 acttacaaag ctaaggagaa gggtgtcaag ttaccaggcg tgcactatgt ggaccacacc
     1441 attgagattt taagccatga caaagattac aacaaggtta agatctatga gtatgctgtt
     1501 gctcattctg gattgcctga caatgccaga cgaggcggta ccggcggatc catggtgagt
     1561 gcgattaagc cagacatgaa gatcaaactg cgtatggaag gcaacgtaaa cgggcaccac
     1621 tttgtgatcg acggagatgg tacaggcaag ccttatgagg gaaaacagac catggatctt
     1681 gaagtcaaag agggcggacc tctgcctttt gcctttgata tcctgaccac tgcattccat
     1741 tacggcaaca gggtattcgt gaaatatcca gacaacatac aagactattt taagcagtcg
     1801 tttcctaagg ggtattcgtg ggaacgaagc atgactttcg aagacggggg catttgctat
     1861 gccagaagcg acataacaat ggaaggggac actttctata ataaagttcg attttatggt
     1921 accaactttc ccgccaatgg tccagttatg cagaagaaga cgctgaaatg gatgccgagc
     1981 tggacgcgtt catcacgtcg taagttcaat aagacaggta aggcactgag agctataggt
     2041 cggctgagct caggaggttc tggaggttct gattacaagg acgacgatga caagggaggt
     2101 tctggaggtt cttacccata cgacgtacca gattacgctg gaggttctgg aggttctgag
     2161 cagaagctga tctcagagga ggacctgcgt acgtaaaagc ttatcgataa tcaacctctg
     2221 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     2281 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     2341 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     2401 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     2461 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     2521 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     2581 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     2641 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     2701 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     2761 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgaccc
     2821 gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
     2881 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     2941 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     3001 gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa
     3061 atcgataagg atcttcctag agcatggcta cgtagataag tagcatggcg ggttaatcat
     3121 taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct
     3181 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt
     3241 gagcgagcga gcgcgcagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     3301 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     3361 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     3421 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     3481 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     3541 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     3601 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     3661 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     3721 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     3781 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3841 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3901 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     3961 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4021 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4081 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     4141 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     4201 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     4261 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     4321 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4381 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4441 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4501 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4561 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4621 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4681 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     4741 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     4801 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     4861 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4921 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4981 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5041 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     5101 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5161 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5221 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5281 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5341 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5401 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5461 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5521 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5581 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     5641 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     5701 agcgaggaag cggaag
//
