LOCUS       Exported                9858 bp ds-DNA     circular SYN 15-AUG-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pVEGFR2_TEV(N)_NES_TCS(Q'L)_FKBP_HA-dCas9(N)_P2A_Puro-WPRE+T274
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9858)
  AUTHORS   Weatherall Institute
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Aug 15, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9858
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    12..810
                     /note="CBh"
     CDS             823..3177
                     /codon_start=1
                     /label=KDR
                     /translation="MQSKVLLAVALWLCVETRAASVGLPSVSLDLPRLSIQKDILTIKA
                     NTTLQITCRGQRDLDWLWPNNQSGSEQRVEVTECSDGLFCKTLTIPKVIGNDTGAYKCF
                     YRETDLASVIYVYVQDYRSPFIASVSDQHGVVYITENKNKTVVIPCLGSISNLNVSLCA
                     RYPEKRFVPDGNRISWDSKKGFTIPSYMISYAGMVFCEAKINDESYQSIMYIVVVVGYR
                     IYDVVLSPSHGIELSVGEKLVLNCTARTELNVGIDFNWEYPSSKHQHKKLVNRDLKTQS
                     GSEMKKFLSTLTIDGITRSDQGLYTCAASSGLMTKKNSTFVRVHEKPFVAFGSGMESLV
                     EATVGERVRIPAKYLGYPPPEIKWYKNGIPLESNHTIKAGHVLTIMEVSERDTGNYTVI
                     LTNPISKEKQSHVVSLVVYVPPQIGEKSLISPVDSYQYGTTQTLTCTVYAIPPPHHIHW
                     YWQLEEECANEPSQAVSVTNPYPCEEWRSVEDFQGGNKIEVNKNQFALIEGKNKTVSTL
                     VIQAANVSALYKCEAVNKVGRGERVISFHVTRGPEITLQPDMQPTEQESVSLWCTADRS
                     TFENLTWYKLGPQPLPIHVGELPTPVCKNLDTLWKLNATMFSNSTNDILIMELKNASLQ
                     DQGDYVCLAQDRKTKKRHCVVRQLTVLERVAPTITGNLENQTTSIGESIEVSCTASGNP
                     PPQIMWFKDNETLVEDSGIVLKDGNRNLTIRRVRKEDEGLYTCQACSVLGCAKVEAFFI
                     IEGAQEKTNLEIIILVGTAVIAMFFWLLLVII"
     CDS             880..3114
                     /product="http://www.uniprot.org/blast/?about=P35968[20-764
                     ]&key=Topological%20domain"
                     /label=ExtracellularSequence
     misc_feature    1711
                     /label=A mutation from Val to Ile (from original vector)
     CDS             3115..3177
                     /product="http://www.uniprot.org/blast/?about=P35968[765-78
                     5]&key=Transmembrane"
                     /label=Transmembrane
     CDS             3202..3555
                     /codon_start=1
                     /label=TEV protease
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQT"
     CDS             3568..3591
                     /codon_start=1
                     /label=NES ptk2
                     /translation="LDLASLIL"
     CDS             3604..3624
                     /codon_start=1
                     /label=TCS (Q'L)
                     /translation="ENLYFQL"
     CDS             3643..3963
                     /codon_start=1
                     /note="FKBP12"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             3970..3996
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4012..5613
                     /codon_start=1
                     /note="hSpCas9"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMR"
     misc_feature    5614..5631
                     /note="(GGGGS)3 linker"
     CDS             5634
                     /codon_start=1
                     /label=rtTA
                     /protein_id="rtTA-M2"
                     /translation=""
     CDS             5641..5700
                     /codon_start=1
                     /label=P2A
                     /translation="GATNFSLLKQAGDVEENPGP"
     CDS             5707..6300
                     /codon_start=1
                     /label=Puro
                     /protein_id="Puro"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKG"
     protein_bind    6337..6864
                     /label=protein bind
     misc_feature    6882..7113
                     /note="bGH polyA"
     misc_feature    7122..7262
                     /label=R-ITR
     rep_origin      7354..7660
                     /direction=RIGHT
                     /label=f1 Origin
     misc_feature    8179..9036
                     /label=AmpR
     rep_origin      9187..9854
                     /direction=RIGHT
                     /label=pUC ori
ORIGIN
        1 ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
       61 ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg tcaatgggtg
      121 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      181 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attgtgccca gtacatgacc
      241 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      301 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      361 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
      421 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      481 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      541 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgac gctgccttcg
      601 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
      661 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agctgagcaa
      721 gaggtaaggg tttaagggat ggttggttgg tggggtatta atgtttaatt acctggagca
      781 cctgcctgaa atcacttttt ttcaggttgg accggtgcca ccatgcagag caaggtgctg
      841 ctggccgtcg ccctgtggct ctgcgtggag acccgggccg cctctgtggg tttgcctagt
      901 gtttctcttg atctgcccag gctcagcata caaaaagaca tacttacaat taaggctaat
      961 acaactcttc aaattacttg caggggacag agggacttgg actggctttg gcccaataat
     1021 cagagtggca gtgagcaaag ggtggaggtg actgagtgca gcgatggcct cttctgtaag
     1081 acactcacaa ttccaaaagt gatcggaaat gacactggag cctacaagtg cttctaccgg
     1141 gaaactgact tggcctcggt catttatgtc tatgttcaag attacagatc tccatttatt
     1201 gcttctgtta gtgaccaaca tggagtcgtg tacattactg agaacaaaaa caaaactgtg
     1261 gtgattccat gtctcgggtc catttcaaat ctcaacgtgt cactttgtgc aagataccca
     1321 gaaaagagat ttgttcctga tggtaacaga atttcctggg acagcaagaa gggctttact
     1381 attcccagct acatgatcag ctatgctggc atggtcttct gtgaagcaaa aattaatgat
     1441 gaaagttacc agtctattat gtacatagtt gtcgttgtag ggtataggat ttatgatgtg
     1501 gttctgagtc cgtctcatgg aattgaacta tctgttggag aaaagcttgt cttaaattgt
     1561 acagcaagaa ctgaactaaa tgtggggatt gacttcaact gggaataccc ttcttcgaag
     1621 catcagcata agaaacttgt aaaccgagac ctaaaaaccc agtctgggag tgagatgaag
     1681 aaatttttga gcaccttaac tatagatggt ataacccgga gtgaccaagg attgtacacc
     1741 tgtgcagcat ccagtgggct gatgaccaag aagaacagca catttgtcag ggtccatgaa
     1801 aaaccttttg ttgcttttgg aagtggcatg gaatctctgg tggaagccac ggtgggggag
     1861 cgtgtcagaa tccctgcgaa gtaccttggt tacccacccc cagaaataaa atggtataaa
     1921 aatggaatac cccttgagtc caatcacaca attaaagcgg ggcatgtact gacgattatg
     1981 gaagtgagtg aaagagacac aggaaattac actgtcatcc ttaccaatcc catttcaaag
     2041 gagaagcaga gccatgtggt ctctctggtt gtgtatgtcc caccccagat tggtgagaaa
     2101 tctctaatct ctcctgtgga ttcctaccag tacggcacca ctcaaacgct gacatgtacg
     2161 gtctatgcca ttcctccccc gcatcacatc cactggtatt ggcagttgga ggaagagtgc
     2221 gccaacgagc ccagccaagc tgtctcagtg acaaacccat acccttgtga agaatggaga
     2281 agtgtggagg acttccaggg aggaaataaa attgaagtta ataaaaatca atttgctcta
     2341 attgaaggaa aaaacaaaac tgtaagtacc cttgttatcc aagcggcaaa tgtgtcagct
     2401 ttgtacaaat gtgaagcggt caacaaagtc gggagaggag agagggtgat ctccttccac
     2461 gtgaccaggg gtcctgaaat tactttgcaa cctgacatgc agcccactga gcaggagagc
     2521 gtgtctttgt ggtgcactgc agacagatct acgtttgaga acctcacatg gtacaagctt
     2581 ggcccacagc ctctgccaat ccatgtggga gagttgccca cacctgtttg caagaacttg
     2641 gatactcttt ggaaattgaa tgccaccatg ttctctaata gcacaaatga cattttgatc
     2701 atggagctta agaatgcatc cttgcaggac caaggagact atgtctgcct tgctcaagac
     2761 aggaagacca agaaaagaca ttgcgtggtc aggcagctca cagtcctaga gcgtgtggca
     2821 cccacgatca caggaaacct ggagaatcag acgacaagta ttggggaaag catcgaagtc
     2881 tcatgcacgg catctgggaa tccccctcca cagatcatgt ggtttaaaga taatgagacc
     2941 cttgtagaag actcaggcat tgtattgaag gatgggaacc ggaacctcac tatccgcaga
     3001 gtgaggaagg aggacgaagg cctctacacc tgccaggcat gcagtgttct tggctgtgca
     3061 aaagtggagg catttttcat aatagaaggt gcccaggaaa agacgaactt ggaaatcatt
     3121 attctagtag gcacggcggt gattgccatg ttcttctggc tacttcttgt catcatcggc
     3181 ggtggctcgg gtggtggctc gggagaaagc ttgtttaagg ggccgcgtga ttacaacccg
     3241 atatcgagca ccatttgtca tttgacgaat gaatctgatg ggcacacaac atcgttgtat
     3301 ggtattggat ttggtccctt catcattaca aacaagcact tgtttagaag aaataatgga
     3361 acactgttgg tccaatcact acatggtgta ttcaaggtca agaacaccac gactttgcaa
     3421 caacacctca ttgatgggag ggacatgata attattcgca tgcctaagga tttcccacca
     3481 tttcctcaaa agctgaaatt tagagagcca caaagggaag agcgcatatg tcttgtgaca
     3541 accaacttcc aaactggcgg tggctcgcta gatttagcta gcttaatcct cggcaagctt
     3601 ggcgaaaacc tgtattttca gctaggcgga ggcagcacta gtggcgtgca ggtggagacc
     3661 atcagccccg gcgacggcag aaccttcccc aagagaggcc agacctgcgt ggtgcactac
     3721 accggcatgc tggaggacgg caagaagttc gacagcagca gagacagaaa caagcccttc
     3781 aagttcatgc tgggcaagca ggaggtgatc agaggctggg aggagggcgt ggcccagatg
     3841 agcgtgggcc agagagccaa gctgaccatc agccccgact acgcctacgg cgccaccggc
     3901 caccccggca tcatccctcc tcacgccacc ctggtgttcg acgtggagct gctgaagctg
     3961 gagactagtt acccatacga cgtaccagat tacgctggag gttctggatc cgacaagaag
     4021 tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag
     4081 tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag
     4141 aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg
     4201 aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag
     4261 atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc
     4321 ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac
     4381 gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac
     4441 agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc
     4501 cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg
     4561 ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc
     4621 ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat
     4681 ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg
     4741 agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg
     4801 cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac
     4861 cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac
     4921 atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga
     4981 tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct
     5041 gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac
     5101 ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac
     5161 ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc
     5221 ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg
     5281 cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg
     5341 accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg
     5401 atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag
     5461 ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac
     5521 gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg
     5581 accaaagtga aatacgtgac cgagggaatg agaggtggcg gtggctcggg taccggatct
     5641 ggagccacca atttttctct gctgaagcag gctggtgatg ttgaagaaaa ccctggacct
     5701 gaattcatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     5761 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     5821 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     5881 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     5941 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     6001 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     6061 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     6121 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     6181 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     6241 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caaggggtaa
     6301 ctaagtaagg atcgatccaa gatatcgtat tcttaactat gttgctcctt ttacgctatg
     6361 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     6421 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     6481 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     6541 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     6601 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     6661 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     6721 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     6781 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     6841 gacgagtcgg atctcccttt gggccgcctc cccgccaatt cctagagctc gctgatcagc
     6901 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     6961 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     7021 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     7081 ggattgggaa gagaatagca ggcatgctgg ggagcggccg caggaacccc tagtgatgga
     7141 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc
     7201 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca
     7261 ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     7321 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     7381 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     7441 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     7501 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
     7561 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     7621 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc
     7681 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     7741 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
     7801 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     7861 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     7921 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     7981 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     8041 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     8101 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     8161 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     8221 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     8281 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     8341 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     8401 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     8461 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     8521 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     8581 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     8641 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     8701 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     8761 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     8821 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     8881 gccggtgagc gtggaagccg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     8941 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     9001 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     9061 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     9121 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     9181 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     9241 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     9301 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     9361 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     9421 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     9481 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     9541 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     9601 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     9661 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     9721 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     9781 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     9841 tggccttttg ctcacatg
//
