LOCUS       Exported                9420 bp ds-DNA     circular SYN 16-AUG-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAVPR2_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1+T273
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9420)
  AUTHORS   Weatherall Institute
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 16, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9420
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    12..810
                     /note="CBh"
     misc_feature    817..824
                     /label=konzak
     CDS             825..905
                     /codon_start=1
                     /product="p.363: At the 5′ end, we included a cleavable 
                     signal sequence to promote membrane localization23 and a 
                     Flag epitope tag to allow monitoring of cell-surface 
                     expression by immunohistochemistry.  -- Highlighted 11 mar 
                     2016
                     flag - DYKDDDDK but where is the ""K""?"
                     /label=membrane localisation signal+ FLAG
                     /translation="MKTIIALSYIFCLVFADYKDDDDASID"
     CDS             906..2018
                     /codon_start=1
                     /label=>AVPR2
                     /translation="MLMASTTSAVPGHPSLPSLPSNSSQERPLDTRDPLLARAELALLS
                     IVFVAVALSNGLVLAALARRGRRGHWAPIHVFIGHLCLADLAVALFQVLPQLAWKATDR
                     FRGPDALCRAVKYLQMVGMYASSYMILAMTLDRHRAICRPMLAYRHGSGAHWNRPVLVA
                     WAFSLLLSLPQLFIFAQRNVEGGSGVTDCWACFAEPWGRRTYVTWIALMVFVAPTLGIA
                     ACQVLIFREIHASLVPGPSERPGGRRRGRRTGSPGEGAHVSAAVAKTVRMTLVIVVVYV
                     LCWAPFFLVQLWAAWDPEAPLEGAPFVLLMLLASLNSCTNPWIYASFSSSVSSELRSLL
                     CCARGRTPPSLGPQDESCTTASSSLAKDTSS"
     CDS             2019..2117
                     /codon_start=1
                     /label=crucial for arrestin recruitment
                     /note="added sequence from the C terminus of the V2 
                     vasopressin receptor (V2 tail) after each recep- tor 
                     sequence, to promote arrestin recruitment20,24–26.         
                                 "
                     /translation="IDTGGRTPPSLGPQDESCTTASSSLAKDTSSTG"
     CDS             2118..2138
                     /codon_start=1
                     /label=efficient TEV cleavage site
                     /translation="ENLYFQG"
     CDS             2139..2159
                     /codon_start=1
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2178..2204
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2205..2213
                     /label=GGS linker
     misc_feature    2220..2231
                     /note="(GGGGS)3 linker"
     CDS             2232..4730
                     /codon_start=1
                     /note="hSpCas9"
                     /translation="KPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEI
                     SGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTY
                     AHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIH
                     DDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPE
                     NIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYL
                     QNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEV
                     VKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQI
                     LDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVV
                     GTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL
                     ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIL
                     PKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME
                     RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALP
                     SKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLD
                     KVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATL
                     IHQSITGLYETRIDLSQLGGD"
     misc_feature    4731..4763
                     /note="NLS"
     CDS             4764..4928
                     /codon_start=1
                     /note="VP64"
                     /translation="GRADALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDAL
                     DDFDLDMLIN"
     CDS             4950..5003
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5016..5405
                     /codon_start=1
                     /label=MCP
                     /translation="MASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKV
                     TCSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCEL
                     IVKAMQGLLKDGNPIPSAIAANSGIY"
     misc_feature    5457..5489
                     /label=NLS
     CDS             5496..6047
                     /codon_start=1
                     /label=P65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISSSGQ"
     CDS             6063..6434
                     /codon_start=1
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     misc_feature    6444..6675
                     /note="bGH polyA"
     misc_feature    6684..6824
                     /label=R-ITR
     rep_origin      6916..7222
                     /direction=RIGHT
                     /label=f1 Origin
     misc_feature    7741..8598
                     /label=AmpR
     rep_origin      8749..9416
                     /direction=RIGHT
                     /label=pUC ori
ORIGIN
        1 ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
       61 ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg tcaatgggtg
      121 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      181 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attgtgccca gtacatgacc
      241 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      301 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      361 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
      421 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      481 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      541 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgac gctgccttcg
      601 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
      661 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agctgagcaa
      721 gaggtaaggg tttaagggat ggttggttgg tggggtatta atgtttaatt acctggagca
      781 cctgcctgaa atcacttttt ttcaggttgg accggtgcgc caccatgaag acgatcatcg
      841 ccctgagcta catcttctgc ctggtattcg ccgactacaa ggacgatgat gacgccagca
      901 tcgatatgct catggcctct accacaagcg ctgtgcccgg acatccatct cttccaagtc
      961 ttccctccaa cagctcacag gagaggcctc tggatacccg ggacccactt cttgcaagag
     1021 cggagctcgc cttgctctcc atcgttttcg ttgccgtcgc gttgagcaat ggtctggtgc
     1081 tggccgccct ggcgcgacga ggccgccgag gccactgggc gcctattcat gtctttatcg
     1141 gccatctctg tcttgcagac ctggccgtgg cgctgtttca ggtgctccct caactggctt
     1201 ggaaagccac cgatagattc cgggggccag acgcactgtg tcgggccgtg aaatacctgc
     1261 agatggtggg aatgtatgcc agttcatata tgatacttgc tatgaccttg gacagacaca
     1321 gggctatctg tcgccccatg ctggcgtata ggcacggttc cggagcccat tggaacaggc
     1381 cagttctggt tgcgtgggct ttttccctgc ttctgagctt gccccagttg tttattttcg
     1441 cacagagaaa tgtggagggc ggatctggcg tgaccgattg ctgggcttgc ttcgctgagc
     1501 catggggcag aaggacctac gtgacgtgga ttgctctcat ggtgttcgtt gctcccaccc
     1561 tggggatcgc cgcatgccag gtcctgatat tcagagaaat acacgcctcc ttggttcctg
     1621 ggccaagcga gcgccctggt ggaagaagac ggggtcgaag gactggctct ccaggcgaag
     1681 gcgcccacgt atcagctgcg gtagctaaga ccgtacgcat gacactggtc atcgtcgtcg
     1741 tttacgttct ttgctgggcc ccgttttttt tggtgcagct ctgggcggcc tgggaccccg
     1801 aagcacccct ggaaggtgct ccattcgttc tgctcatgct tctcgcttca ctgaatagct
     1861 gtaccaatcc ttggatttac gcctctttct cctccagtgt tagctccgag ctgcggagtc
     1921 ttctctgttg cgcccggggc aggacccccc caagcctggg tccacaggac gaatcctgca
     1981 ctaccgcctc cagttccctg gccaaagaca cctcttctat cgataccggt ggacgcaccc
     2041 cacccagcct gggtccccaa gatgagtcct gcaccaccgc cagctcctcc ctggccaagg
     2101 acacttcatc gaccggtgag aacctgtact tccagggccc caagaagaag aggaaggtgg
     2161 ggggaggcag cactagttac ccatacgacg taccagatta cgctggaggt tctggatccg
     2221 gcggtggctc gaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc
     2281 tgttcaagac caaccggaaa gtgaccgtga agcagctgaa agaggactac ttcaagaaaa
     2341 tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga tcggttcaac gcctccctgg
     2401 gcacatacca cgatctgctg aaaattatca aggacaagga cttcctggac aatgaggaaa
     2461 acgaggacat tctggaagat atcgtgctga ccctgacact gtttgaggac agagagatga
     2521 tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga caaagtgatg aagcagctga
     2581 agcggcggag atacaccggc tggggcaggc tgagccggaa gctgatcaac ggcatccggg
     2641 acaagcagtc cggcaagaca atcctggatt tcctgaagtc cgacggcttc gccaacagaa
     2701 acttcatgca gctgatccac gacgacagcc tgacctttaa agaggacatc cagaaagccc
     2761 aggtgtccgg ccagggcgat agcctgcacg agcacattgc caatctggcc ggcagccccg
     2821 ccattaagaa gggcatcctg cagacagtga aggtggtgga cgagctcgtg aaagtgatgg
     2881 gccggcacaa gcccgagaac atcgtgatcg aaatggccag agagaaccag accacccaga
     2941 agggacagaa gaacagccgc gagagaatga agcggatcga agagggcatc aaagagctgg
     3001 gcagccagat cctgaaagaa caccccgtgg aaaacaccca gctgcagaac gagaagctgt
     3061 acctgtacta cctgcagaat gggcgggata tgtacgtgga ccaggaactg gacatcaacc
     3121 ggctgtccga ctacgatgtg gaccatatcg tgcctcagag ctttctgaag gacgactcca
     3181 tcgacaacaa ggtgctgacc agaagcgaca aggcccgggg caagagcgac aacgtgccct
     3241 ccgaagaggt cgtgaagaag atgaagaact actggcggca gctgctgaac gccaagctga
     3301 ttacccagag aaagttcgac aatctgacca aggccgagag aggcggcctg agcgaactgg
     3361 ataaggccgg cttcatcaag agacagctgg tggaaacccg gcagatcaca aagcacgtgg
     3421 cacagatcct ggactcccgg atgaacacta agtacgacga gaatgacaag ctgatccggg
     3481 aagtgaaagt gatcaccctg aagtccaagc tggtgtccga tttccggaag gatttccagt
     3541 tttacaaagt gcgcgagatc aacaactacc accacgccca cgacgcctac ctgaacgccg
     3601 tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg
     3661 actacaaggt gtacgacgtg cggaagatga tcgccaagag cgagcaggaa atcggcaagg
     3721 ctaccgccaa gtacttcttc tacagcaaca tcatgaactt tttcaagacc gagattaccc
     3781 tggccaacgg cgagatccgg aagcggcctc tgatcgagac aaacggcgaa accggggaga
     3841 tcgtgtggga taagggccgg gattttgcca ccgtgcggaa agtgctgagc atgccccaag
     3901 tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt cagcaaagag tctatcctgc
     3961 ccaagaggaa cagcgataag ctgatcgcca gaaagaagga ctgggaccct aagaagtacg
     4021 gcggcttcga cagccccacc gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg
     4081 gcaagtccaa gaaactgaag agtgtgaaag agctgctggg gatcaccatc atggaaagaa
     4141 gcagcttcga gaagaatccc atcgactttc tggaagccaa gggctacaaa gaagtgaaaa
     4201 aggacctgat catcaagctg cctaagtact ccctgttcga gctggaaaac ggccggaaga
     4261 gaatgctggc ctctgccggc gaactgcaga agggaaacga actggccctg ccctccaaat
     4321 atgtgaactt cctgtacctg gccagccact atgagaagct gaagggctcc cccgaggata
     4381 atgagcagaa acagctgttt gtggaacagc acaagcacta cctggacgag atcatcgagc
     4441 agatcagcga gttctccaag agagtgatcc tggccgacgc taatctggac aaagtgctgt
     4501 ccgcctacaa caagcaccgg gataagccca tcagagagca ggccgagaat atcatccacc
     4561 tgtttaccct gaccaatctg ggagcccctg ccgccttcaa gtactttgac accaccatcg
     4621 accggaagag gtacaccagc accaaagagg tgctggacgc caccctgatc caccagagca
     4681 tcaccggcct gtacgagaca cggatcgacc tgtctcagct gggaggcgac agccccaaga
     4741 agaagagaaa ggtggaggcc agcggacggg ctgacgcatt ggacgatttt gatctggata
     4801 tgctgggaag tgacgccctc gatgattttg accttgacat gcttggttcg gatgcccttg
     4861 atgactttga cctcgacatg ctcggcagtg acgcccttga tgatttcgac ctggacatgc
     4921 tgattaacgg taccgctagc ggcagtggag agggcagagg aagtctgcta acatgcggtg
     4981 acgtcgagga gaatcctggc ccagtgagca agcttatggc ttcaaacttt actcagttcg
     5041 tgctcgtgga caatggtggg acaggggatg tgacagtggc tccttctaat ttcgctaatg
     5101 gggtggcaga gtggatcagc tccaactcac ggagccaggc ctacaaggtg acatgcagcg
     5161 tcaggcagtc tagtgcccag aagagaaagt ataccatcaa ggtggaggtc cccaaagtgg
     5221 ctacccagac agtgggcgga gtcgaactgc ctgtcgccgc ttggaggtcc tacctgaaca
     5281 tggagctcac tatcccaatt ttcgctacca attctgactg tgaactcatc gtgaaggcaa
     5341 tgcaggggct cctcaaagac ggtaatccta tcccttccgc catcgccgct aactcaggta
     5401 tctacagcgc tggaggaggt ggaagcggag gaggaggaag cggaggagga ggtagcggac
     5461 ctaagaaaaa gaggaaggtg gcggccgctg gatccccttc agggcagatc agcaaccagg
     5521 ccctggctct ggcccctagc tccgctccag tgctggccca gactatggtg ccctctagtg
     5581 ctatggtgcc tctggcccag ccacctgctc cagcccctgt gctgacccca ggaccacccc
     5641 agtcactgag cgctccagtg cccaagtcta cacaggccgg cgaggggact ctgagtgaag
     5701 ctctgctgca cctgcagttc gacgctgatg aggacctggg agctctgctg gggaacagca
     5761 ccgatcccgg agtgttcaca gatctggcct ccgtggacaa ctctgagttt cagcagctgc
     5821 tgaatcaggg cgtgtccatg tctcatagta cagccgaacc aatgctgatg gagtaccccg
     5881 aagccattac ccggctggtg accggcagcc agcggccccc cgaccccgct ccaactcccc
     5941 tgggaaccag cggcctgcct aatgggctgt ccggagatga agacttctca agcatcgctg
     6001 atatggactt tagtgccctg ctgtcacaga tttcctctag tgggcaggga ggaggtggaa
     6061 gcggcttcag cgtggacacc agtgccctgc tggacctgtt cagcccctcg gtgaccgtgc
     6121 ccgacatgag cctgcctgac cttgacagca gcctggccag tatccaagag ctcctgtctc
     6181 cccaggagcc ccccaggcct cccgaggcag agaacagcag cccggattca gggaagcagc
     6241 tggtgcacta cacagcgcag ccgctgttcc tgctggaccc cggctccgtg gacaccggga
     6301 gcaacgacct gccggtgctg tttgagctgg gagagggctc ctacttctcc gaaggggacg
     6361 gcttcgccga ggaccccacc atctccctgc tgacaggctc ggagcctccc aaagccaagg
     6421 accccactgt ctcctaagaa ttcctagagc tcgctgatca gcctcgactg tgccttctag
     6481 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     6541 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     6601 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagagaatag
     6661 caggcatgct ggggagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
     6721 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     6781 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
     6841 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     6901 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     6961 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     7021 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     7081 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     7141 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     7201 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa
     7261 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     7321 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     7381 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     7441 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     7501 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     7561 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     7621 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     7681 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     7741 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     7801 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     7861 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     7921 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     7981 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     8041 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     8101 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     8161 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     8221 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     8281 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     8341 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     8401 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggaagc
     8461 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     8521 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     8581 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     8641 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     8701 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     8761 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     8821 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     8881 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     8941 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     9001 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     9061 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     9121 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     9181 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     9241 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     9301 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     9361 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
//
