LOCUS       Exported                9363 bp ds-DNA     circular SYN 16-AUG-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCXCR4_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1+T283
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9363)
  AUTHORS   Weatherall Institute
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 16, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9363
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    12..810
                     /note="CBh"
     misc_feature    817..824
                     /label=konzak
     CDS             825..905
                     /codon_start=1
                     /product="p.363: At the 5′ end, we included a cleavable 
                     signal sequence to promote membrane localization23 and a 
                     Flag epitope tag to allow monitoring of cell-surface 
                     expression by immunohistochemistry.  -- Highlighted 11 mar 
                     2016
                     flag - DYKDDDDK but where is the ""K""?"
                     /label=membrane localisation signal+ FLAG
                     /translation="MKTIIALSYIFCLVFADYKDDDDASID"
     CDS             906..1961
                     /codon_start=1
                     /label=CXCR4
                     /translation="MEGISIYTSDNYTEEMGSGDYDSMKEPCFREENANFNKIFLPTIY
                     SIIFLTGIVGNGLVILVMGYQKKLRSMTDKYRLHLSVADLLFVITLPFWAVDAVANWYF
                     GNFLCKAVHVIYTVNLYSSVLILAFISLDRYLAIVHATNSQRPRKLLAEKVVYVGVWIP
                     ALLLTIPDFIFANVSEADDRYICDRFYPNDLWVVVFQFQHIMVGLILPGIVILSCYCII
                     ISKLSHSKGHQKRKALKTTVILILAFFACWLPYYIGISIDSFILLEIIKQGCEFENTVH
                     KWISITEALAFFHCCLNPILYAFLGAKFKTSAQHALTSVSRGSSLKILSKGKRGGHSSV
                     STESESSSFHSS"
     CDS             1962..2060
                     /codon_start=1
                     /label=crucial for arrestin recruitment
                     /note="added sequence from the C terminus of the V2 
                     vasopressin receptor (V2 tail) after each recep- tor 
                     sequence, to promote arrestin recruitment20,24–26.         
                                 "
                     /translation="IDTGGRTPPSLGPQDESCTTASSSLAKDTSSTG"
     CDS             2061..2081
                     /codon_start=1
                     /label=efficient TEV cleavage site
                     /translation="ENLYFQG"
     CDS             2082..2102
                     /codon_start=1
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2121..2147
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2148..2156
                     /label=GGS linker
     misc_feature    2163..2174
                     /note="(GGGGS)3 linker"
     CDS             2175..4673
                     /codon_start=1
                     /note="hSpCas9"
                     /translation="KPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEI
                     SGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTY
                     AHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIH
                     DDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPE
                     NIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYL
                     QNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEV
                     VKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQI
                     LDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVV
                     GTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL
                     ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIL
                     PKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME
                     RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALP
                     SKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLD
                     KVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATL
                     IHQSITGLYETRIDLSQLGGD"
     misc_feature    4674..4706
                     /note="NLS"
     CDS             4707..4871
                     /codon_start=1
                     /note="VP64"
                     /translation="GRADALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDAL
                     DDFDLDMLIN"
     CDS             4893..4946
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4959..5348
                     /codon_start=1
                     /label=MCP
                     /translation="MASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKV
                     TCSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCEL
                     IVKAMQGLLKDGNPIPSAIAANSGIY"
     misc_feature    5400..5432
                     /label=NLS
     CDS             5439..5990
                     /codon_start=1
                     /label=P65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISSSGQ"
     CDS             6006..6377
                     /codon_start=1
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     misc_feature    6387..6618
                     /note="bGH polyA"
     misc_feature    6627..6767
                     /label=R-ITR
     rep_origin      6859..7165
                     /direction=RIGHT
                     /label=f1 Origin
     misc_feature    7684..8541
                     /label=AmpR
     rep_origin      8692..9359
                     /direction=RIGHT
                     /label=pUC ori
ORIGIN
        1 ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
       61 ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg tcaatgggtg
      121 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      181 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attgtgccca gtacatgacc
      241 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      301 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      361 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
      421 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      481 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      541 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgac gctgccttcg
      601 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
      661 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agctgagcaa
      721 gaggtaaggg tttaagggat ggttggttgg tggggtatta atgtttaatt acctggagca
      781 cctgcctgaa atcacttttt ttcaggttgg accggtgcgc caccatgaag acgatcatcg
      841 ccctgagcta catcttctgc ctggtattcg ccgactacaa ggacgatgat gacgccagca
      901 tcgatatgga ggggatatca atctacacat cagataatta cacggaagag atgggttccg
      961 gcgattacga ctctatgaaa gagccgtgtt ttagagagga aaacgcgaac tttaacaaga
     1021 tctttctccc caccatctac agcatcatct ttctcacagg catcgtaggg aacggcctgg
     1081 tcatcctggt gatgggatac caaaagaagc tgaggtcaat gaccgacaag tataggctcc
     1141 atctgtccgt ggccgacctc ctgtttgtga ttaccctgcc tttttgggca gttgacgctg
     1201 tcgctaattg gtacttcggc aacttcctct gtaaggcagt gcacgttatc tacactgtga
     1261 atctttatag ttccgtcttg atcttggcct ttatcagtct cgacaggtat ttggcgattg
     1321 tgcacgctac caactcacaa cgacctagaa aactcctggc tgagaaggtg gtttacgttg
     1381 gtgtgtggat tccagctctc ctgttgacaa taccagactt cattttcgct aatgtgagcg
     1441 aggccgatga cagatacatt tgtgaccgat tttacccaaa cgatctgtgg gtagtggtat
     1501 ttcagttcca acacattatg gtcgggctga tcttgcccgg cattgtcata ctgtcttgct
     1561 actgcatcat tatttctaag ctgtcacact caaaaggcca ccaaaagagg aaggctctga
     1621 aaacaacggt gatcctgata ctggccttct tcgcatgttg gctgccctac tatatcggca
     1681 tcagcattga ctcatttata ctcctggaaa ttatcaagca gggctgcgag ttcgagaaca
     1741 ccgttcataa gtggatttct ataaccgagg ccctcgcctt ctttcactgt tgtttgaatc
     1801 cgattctcta cgcgtttctt ggcgccaaat ttaaaacaag cgcccaacat gcactgacat
     1861 cagtgtctag ggggagctct ctgaaaatcc tctccaaggg aaaacgaggc ggacatagca
     1921 gtgtcagcac tgagtccgaa tccagctcat ttcatagctc tatcgatacc ggtggacgca
     1981 ccccacccag cctgggtccc caagatgagt cctgcaccac cgccagctcc tccctggcca
     2041 aggacacttc atcgaccggt gagaacctgt acttccaggg ccccaagaag aagaggaagg
     2101 tggggggagg cagcactagt tacccatacg acgtaccaga ttacgctgga ggttctggat
     2161 ccggcggtgg ctcgaagccc gccttcctga gcggcgagca gaaaaaggcc atcgtggacc
     2221 tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac tacttcaaga
     2281 aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc
     2341 tgggcacata ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg
     2401 aaaacgagga cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga
     2461 tgatcgagga acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc
     2521 tgaagcggcg gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc
     2581 gggacaagca gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca
     2641 gaaacttcat gcagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag
     2701 cccaggtgtc cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc
     2761 ccgccattaa gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga
     2821 tgggccggca caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc
     2881 agaagggaca gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc
     2941 tgggcagcca gatcctgaaa gaacaccccg tggaaaacac ccagctgcag aacgagaagc
     3001 tgtacctgta ctacctgcag aatgggcggg atatgtacgt ggaccaggaa ctggacatca
     3061 accggctgtc cgactacgat gtggaccata tcgtgcctca gagctttctg aaggacgact
     3121 ccatcgacaa caaggtgctg accagaagcg acaaggcccg gggcaagagc gacaacgtgc
     3181 cctccgaaga ggtcgtgaag aagatgaaga actactggcg gcagctgctg aacgccaagc
     3241 tgattaccca gagaaagttc gacaatctga ccaaggccga gagaggcggc ctgagcgaac
     3301 tggataaggc cggcttcatc aagagacagc tggtggaaac ccggcagatc acaaagcacg
     3361 tggcacagat cctggactcc cggatgaaca ctaagtacga cgagaatgac aagctgatcc
     3421 gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc cgatttccgg aaggatttcc
     3481 agttttacaa agtgcgcgag atcaacaact accaccacgc ccacgacgcc tacctgaacg
     3541 ccgtcgtggg aaccgccctg atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg
     3601 gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca
     3661 aggctaccgc caagtacttc ttctacagca acatcatgaa ctttttcaag accgagatta
     3721 ccctggccaa cggcgagatc cggaagcggc ctctgatcga gacaaacggc gaaaccgggg
     3781 agatcgtgtg ggataagggc cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc
     3841 aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa gagtctatcc
     3901 tgcccaagag gaacagcgat aagctgatcg ccagaaagaa ggactgggac cctaagaagt
     3961 acggcggctt cgacagcccc accgtggcct attctgtgct ggtggtggcc aaagtggaaa
     4021 agggcaagtc caagaaactg aagagtgtga aagagctgct ggggatcacc atcatggaaa
     4081 gaagcagctt cgagaagaat cccatcgact ttctggaagc caagggctac aaagaagtga
     4141 aaaaggacct gatcatcaag ctgcctaagt actccctgtt cgagctggaa aacggccgga
     4201 agagaatgct ggcctctgcc ggcgaactgc agaagggaaa cgaactggcc ctgccctcca
     4261 aatatgtgaa cttcctgtac ctggccagcc actatgagaa gctgaagggc tcccccgagg
     4321 ataatgagca gaaacagctg tttgtggaac agcacaagca ctacctggac gagatcatcg
     4381 agcagatcag cgagttctcc aagagagtga tcctggccga cgctaatctg gacaaagtgc
     4441 tgtccgccta caacaagcac cgggataagc ccatcagaga gcaggccgag aatatcatcc
     4501 acctgtttac cctgaccaat ctgggagccc ctgccgcctt caagtacttt gacaccacca
     4561 tcgaccggaa gaggtacacc agcaccaaag aggtgctgga cgccaccctg atccaccaga
     4621 gcatcaccgg cctgtacgag acacggatcg acctgtctca gctgggaggc gacagcccca
     4681 agaagaagag aaaggtggag gccagcggac gggctgacgc attggacgat tttgatctgg
     4741 atatgctggg aagtgacgcc ctcgatgatt ttgaccttga catgcttggt tcggatgccc
     4801 ttgatgactt tgacctcgac atgctcggca gtgacgccct tgatgatttc gacctggaca
     4861 tgctgattaa cggtaccgct agcggcagtg gagagggcag aggaagtctg ctaacatgcg
     4921 gtgacgtcga ggagaatcct ggcccagtga gcaagcttat ggcttcaaac tttactcagt
     4981 tcgtgctcgt ggacaatggt gggacagggg atgtgacagt ggctccttct aatttcgcta
     5041 atggggtggc agagtggatc agctccaact cacggagcca ggcctacaag gtgacatgca
     5101 gcgtcaggca gtctagtgcc cagaagagaa agtataccat caaggtggag gtccccaaag
     5161 tggctaccca gacagtgggc ggagtcgaac tgcctgtcgc cgcttggagg tcctacctga
     5221 acatggagct cactatccca attttcgcta ccaattctga ctgtgaactc atcgtgaagg
     5281 caatgcaggg gctcctcaaa gacggtaatc ctatcccttc cgccatcgcc gctaactcag
     5341 gtatctacag cgctggagga ggtggaagcg gaggaggagg aagcggagga ggaggtagcg
     5401 gacctaagaa aaagaggaag gtggcggccg ctggatcccc ttcagggcag atcagcaacc
     5461 aggccctggc tctggcccct agctccgctc cagtgctggc ccagactatg gtgccctcta
     5521 gtgctatggt gcctctggcc cagccacctg ctccagcccc tgtgctgacc ccaggaccac
     5581 cccagtcact gagcgctcca gtgcccaagt ctacacaggc cggcgagggg actctgagtg
     5641 aagctctgct gcacctgcag ttcgacgctg atgaggacct gggagctctg ctggggaaca
     5701 gcaccgatcc cggagtgttc acagatctgg cctccgtgga caactctgag tttcagcagc
     5761 tgctgaatca gggcgtgtcc atgtctcata gtacagccga accaatgctg atggagtacc
     5821 ccgaagccat tacccggctg gtgaccggca gccagcggcc ccccgacccc gctccaactc
     5881 ccctgggaac cagcggcctg cctaatgggc tgtccggaga tgaagacttc tcaagcatcg
     5941 ctgatatgga ctttagtgcc ctgctgtcac agatttcctc tagtgggcag ggaggaggtg
     6001 gaagcggctt cagcgtggac accagtgccc tgctggacct gttcagcccc tcggtgaccg
     6061 tgcccgacat gagcctgcct gaccttgaca gcagcctggc cagtatccaa gagctcctgt
     6121 ctccccagga gccccccagg cctcccgagg cagagaacag cagcccggat tcagggaagc
     6181 agctggtgca ctacacagcg cagccgctgt tcctgctgga ccccggctcc gtggacaccg
     6241 ggagcaacga cctgccggtg ctgtttgagc tgggagaggg ctcctacttc tccgaagggg
     6301 acggcttcgc cgaggacccc accatctccc tgctgacagg ctcggagcct cccaaagcca
     6361 aggaccccac tgtctcctaa gaattcctag agctcgctga tcagcctcga ctgtgccttc
     6421 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     6481 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     6541 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagagaa
     6601 tagcaggcat gctggggagc ggccgcagga acccctagtg atggagttgg ccactccctc
     6661 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt
     6721 tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg
     6781 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag
     6841 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     6901 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6961 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     7021 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
     7081 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     7141 ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta
     7201 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     7261 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc
     7321 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     7381 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     7441 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     7501 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     7561 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7621 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7681 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7741 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7801 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7861 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     7921 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     7981 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     8041 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     8101 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     8161 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     8221 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     8281 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     8341 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtgga
     8401 agccgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     8461 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8521 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8581 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     8641 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8701 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8761 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8821 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     8881 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     8941 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     9001 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     9061 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     9121 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     9181 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     9241 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     9301 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     9361 atg
//
