LOCUS       Exported                5802 bp ds-DNA     circular SYN 14-AUG-2017
DEFINITION  Cloning vector pSNAP-tagm, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    Untitled 13
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Brecht A, Muentner K.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2010) Research Department, New England Biolabs, 
            240 County Road, Ipswich, MA 01938, USA
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Addgene
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 14, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Cloning vector pSNAP-tagm"
                     /mol_type="other DNA"
     promoter        251..818
                     /note="CMV immediate early promoter region"
     promoter        863..880
                     /note="T7 promoter"
     misc_feature    909..923
                     /note="multiple cloning site MCS1 (ClaI-EcoRI)"
     CDS             927..1508
                     /codon_start=1
                     /gene="SNAP26m"
                     /product="O-6-methylguanine-DNA methyltransferase"
                     /note="SNAP26m"
                     /note="SNAP26m sequence 927..1472, vector-encoded sequence 
                     1473..1508 (encoding C-terminal residues PAGIGAPGSLE); 
                     optimized for mammalian expression; possible alternative 
                     start 774"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYQQLAALAGNPAATAAVKTALSGNPVPILIPCHRVVSSSGAVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLGPAGIGAPGSLE"
     gene            927..1508
                     /gene="SNAP26m"
                     /note="SNAP26m"
     misc_feature    1473..1517
                     /note="multiple cloning site MCS2 (SbfI-NotI)"
     misc_RNA        1886..2468
                     /note="internal ribosome entry site (IRES) from 
                     encephalomyocarditis virus (ECMV); allows polycistronic 
                     expression of SNAP26m and neoR, and required for expression
                     of neoR"
     CDS             2489..3292
                     /codon_start=1
                     /gene="aph(3')-II"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="aph(3')-II"
                     /note="neoR, kanR, nptII (confers resistance to neomycin 
                     and kanamycin) (mutant R177S); possible alternative start 
                     at 2363"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            2489..3292
                     /gene="aph(3')-II"
                     /note="aph(3')-II"
     promoter        complement(3694..3723)
                     /note="Plac promoter (-35 signal TTTACA, -10 signal 
                     TATGTT)"
     rep_origin      complement(4047..4635)
                     /direction=LEFT
                     /note="pUC19 origin of replication (counter-clockwise) 
                     (RNAII -35 to RNA/DNA switch point)"
     CDS             complement(4806..5666)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="bla"
                     /note="ampR (confers resistance to ampicillin) (mutant 
                     V82I,A182V)"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(4806..5666)
                     /gene="bla"
                     /note="bla"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat cgatatcgaa ttcaccatgg acaaagactg cgaaatgaag cgcaccaccc
      961 tggatagccc tctgggcaag ctggaactgt ctgggtgcga acagggcctg cacgagatca
     1021 agctgctggg caaaggaaca tctgccgccg acgccgtgga agtgcctgcc ccagccgccg
     1081 tgctgggcgg accagagcca ctgatgcagg ccaccgcctg gctcaacgcc tactttcacc
     1141 agcctgaggc catcgaggag ttccctgtgc cagccctgca ccacccagtg ttccagcagg
     1201 agagctttac ccgccaggtg ctgtggaaac tgctgaaagt ggtgaagttc ggagaggtca
     1261 tcagctacca gcagctggcc gccctggccg gcaatcccgc cgccaccgcc gccgtgaaaa
     1321 ccgccctgag cggaaatccc gtgcccattc tgatcccctg ccaccgggtg gtgtctagct
     1381 ctggcgccgt ggggggctac gagggcgggc tcgccgtgaa agagtggctg ctggcccacg
     1441 agggccacag actgggcaag cctgggctgg gtcctgcagg tataggcgcg ccaggatccc
     1501 tcgagtgagg cggccgcata gataactgat ccagtgtgct ggaattaatt cgctgtctgc
     1561 gagggccagc tgttggggtg agtactccct ctcaaaagcg ggcatgactt ctgcgctaag
     1621 attgtcagtt tccaaaaacg aggaggattt gatattcacc tggcccgcgg tgatgccttt
     1681 gagggtggcc gcgtccatct ggtcagaaaa gacaatcttt ttgttgtcaa gcttgaggtg
     1741 tggcaggctt gagatctggc catacacttg agtgacaatg acatccactt tgcctttctc
     1801 tccacaggtg tccactccca ggtccaactg caggtcgagc atgcatctag ggcggccaat
     1861 tccgcccctc tccccccccc ccttttccct cccccccccc taacgttact ggccgaagcc
     1921 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     1981 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     2041 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     2101 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     2161 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     2221 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     2281 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     2341 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     2401 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc
     2461 acaacccggg ataattcctg cagccaatat gggatcggcc attgaacaag atggattgca
     2521 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     2581 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     2641 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     2701 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     2761 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     2821 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     2881 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     2941 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     3001 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca
     3061 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     3121 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     3181 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     3241 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaggggatca
     3301 attctctaga taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt
     3361 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     3421 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     3481 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     3541 ttaacgcgtc gagtgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     3601 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     3661 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     3721 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     3781 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     3841 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     3901 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3961 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4021 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4081 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4141 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4201 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     4261 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4321 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4381 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4441 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     4501 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4561 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4621 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4681 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4741 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4801 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4861 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     4921 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     4981 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     5041 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     5101 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     5161 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     5221 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     5281 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     5341 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5401 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     5461 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     5521 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     5581 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     5641 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     5701 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     5761 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
