LOCUS       Exported                3725 bp ds-DNA     circular SYN 14-AUG-2017
DEFINITION  Cloning vector pSNAP-tagT7, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    Untitled 15
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Brecht A, Muentner K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2010) Research Department, New England Biolabs, 
            240 County Road, Ipswich, MA 01938, USA
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Addgene
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 14, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Cloning vector pSNAP-tagT7"
                     /mol_type="other DNA"
     rep_origin      complement(16..461)
                     /direction=LEFT
                     /note="f1 origin of replication (+ -)"
     promoter        629..646
                     /note="T7 promoter"
     RBS             714..719
                     /note="for translation of SNAP26b"
     CDS             728..1303
                     /codon_start=1
                     /gene="SNAP26b"
                     /product="O-6-methylguanine-DNA methyltransferase"
                     /note="SNAP26b"
                     /note="optimized for bacterial expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYQQLAALAGNPAATAAVKTALSGNPVPILIPCHRVVSSSGAVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLGPAGIGAPGS"
     gene            728..1303
                     /gene="SNAP26b"
                     /note="SNAP26b"
     misc_feature    1275..1342
                     /note="multiple cloning site (PstI-XhoI)"
     terminator      1352..1474
                     /note="T7 Tphi transcription terminator"
     promoter        complement(1628..1657)
                     /note="Plac promoter (-35 signal TTTACA, -10 signal 
                     TATGTT)"
     rep_origin      complement(1981..2569)
                     /direction=LEFT
                     /note="pUC19 origin of replication (counter-clockwise) 
                     (RNAII -35 to RNA/DNA switch point)"
     CDS             complement(2740..3600)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="bla"
                     /note="ampR (confers resistance to ampicillin) (mutant 
                     V82I,A182V)"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2740..3600)
                     /gene="bla"
                     /note="bla"
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac
      661 cgggcccaga ccacaacggt ttccctctag aaataatttt gtttaacttt aagaaggaga
      721 tatacatatg gacaaagatt gcgaaatgaa acgtaccacc ctggatagcc cgctgggcaa
      781 actggaactg agcggctgcg aacagggcct gcatgaaatt aaactgctgg gtaaaggcac
      841 cagcgcggcc gatgcggttg aagttccggc cccggccgcc gtgctgggtg gtccggaacc
      901 gctgatgcag gcgaccgcgt ggctgaacgc gtattttcat cagccggaag cgattgaaga
      961 atttccggtt ccggcgctgc atcatccggt gtttcagcag gagagcttta cccgtcaggt
     1021 gctgtggaaa ctgctgaaag tggttaaatt tggcgaagtg attagctatc agcagctggc
     1081 ggccctggcg ggtaatccgg cggccaccgc cgccgttaaa accgcgctga gcggtaaccc
     1141 ggtgccgatt ctgattccgt gccatcgtgt ggttagctct agcggtgcgg ttggcggtta
     1201 tgaaggtggt ctggcggtga aagagtggct gctggcccat gaaggtcatc gtctgggtaa
     1261 accgggtctg ggacctgcag gtataggcgc gccaggatcc taagaattct gcagatatcc
     1321 atcacactgg cggccgctcg agcagatccg gctgctaaca aagcccgaaa ggaagctgag
     1381 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
     1441 ttgaggggtt ttttgctgaa aggaggaact atatccggat aatctagagg cactagttct
     1501 agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc
     1561 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     1621 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     1681 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     1741 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     1801 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1861 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1921 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1981 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2041 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2101 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2161 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2221 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     2281 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2341 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2401 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     2461 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2521 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2581 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2641 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2701 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2761 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2821 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2881 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2941 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3001 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3061 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3121 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3181 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3241 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3301 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3361 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3421 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3481 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3541 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3601 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3661 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3721 agtgc
//
