LOCUS       pBABE_kappa_HA_d        7881 bp ds-DNA     circular     20-FEB-2018
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     rep_origin      complement(6167..6849)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3978..4046
                     /label=LacZ alpha
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(6954..7613)
                     /label=AmpR
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1573..2292
                     /label=dl5-NP138
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1468..1530
                     /label=Igk leader sequence
                     /ApEinfo_fwdcolor="#ffc90c"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1531..1557
                     /label=HA epitope
                     /ApEinfo_fwdcolor="#ff34af"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2329..2358
                     /label=c-myc epitope
                     /ApEinfo_fwdcolor="#c777ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2365..3606
                     /label=ADRB2-sequence
                     /ApEinfo_fwdcolor="#2b42ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4522..5121
                     /label=Puro-Resistance
                     /ApEinfo_fwdcolor="#ff2f10"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     LTR             76..537
                     /label=MoMuLv LTR
                     /ApEinfo_fwdcolor="#12ff29"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     LTR             5352..5813
                     /label=MoMuLv LTR(1)
                     /ApEinfo_label="MoMuLv LTR"
                     /ApEinfo_fwdcolor="#12ff29"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1573..1902
                     /label=L5(138)monomer-N-side
                     /ApEinfo_fwdcolor="#b9fff6"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1963..2292
                     /label=L5(138)monomer-C-side
                     /ApEinfo_fwdcolor="#b4fcff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1903..1962
                     /label=(G4S)4 dimer linker
                     /ApEinfo_fwdcolor="#a6f3ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
       61 gcagcctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
      121 gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
      181 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt
      241 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
      301 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
      361 tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc
      421 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
      481 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg
      541 gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggta
      601 agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac tgattttatg
      661 cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact
      721 gacgagttct gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt
      781 ttttgtggcc cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg
      841 gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg
      901 gtttggaacc gaagccgcgc gtcttgtctg ctgcagcatc gttctgtgtt gtctctgtct
      961 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
     1021 gaccttagat cactggaaag atgtcgagcg gctcgctcac aaccagtcgg tagatgtcaa
     1081 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
     1141 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
     1201 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
     1261 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcttcc
     1321 atccgcgccg tctctccccc ttgaacctcc tctttcgacc ccgcctcaat cctcccttta
     1381 tccagccctc actccttctc taggcgccgg ccggatccac tagtaacggc cgccagtgtg
     1441 ctggaattcg gcttggggat atccaccATG GAGACAGACA CACTCCTGCT ATGGGTACTG
     1501 CTGCTCTGGG TTCCAGGTTC CACTGGTGAC TATCCATATG ATGTTCCAGA TTATGCTGGG
     1561 GCCCAGCCGG CCCAGGCCGT CGTTACCCAA GAACCTAGTG TTACCGTTAG CCCAGGTGGT
     1621 ACTGTTATAC TTACTTGTGG AAGTGGTACG GGTGCCGTCA CATCTGGTCA TTATGCAAAT
     1681 TGGTTTCAAC AAAAACCAGG ACAAGCTCCA AGAGCTTTGA TTTTTGATAC TGATAAGAAG
     1741 TATTCTTGGA CCCCAGGTAG ATTTTCTGGA TCTTTGCTGG GAGCAAAGGC AGCTTTGACA
     1801 ATATCAGATG CTCAGCCTGA GGACGAAGCC GAGTATTACT GTTCTCTTAG CGACGTGGAT
     1861 GGCTACTTGT TTGGCGGTGG AACACAACTG ACGGTTCTGT CCGGTGGTGG CGGCTCTGGT
     1921 GGCGGTGGCA GCGGCGGTGG TGGTTCCGGA GGCGGCGGTT CTCAGGCTGT GGTGACTCAG
     1981 GAGCCGTCAG TGACTGTGTC CCCAGGAGGG ACAGTCATTC TCACTTGTGG CTCCGGCACT
     2041 GGAGCTGTCA CCAGTGGTCA TTATGCCAAC TGGTTCCAGC AGAAGCCTGG CCAAGCCCCC
     2101 AGGGCACTTA TATTTGACAC CGACAAGAAG TATTCCTGGA CCCCTGGCCG ATTCTCAGGC
     2161 TCCCTCCTTG GGGCCAAGGC TGCCCTGACC ATCTCGGATG CGCAGCCTGA AGATGAGGCT
     2221 GAGTATTACT GTTCGCTCTC CGACGTTGAC GGTTATCTGT TCGGAGGAGG CACCCAGCTG
     2281 ACCGTCCTCT CCGGCCGCAG GGGCCGGGAT CCGCGGCTGC AGGTCGACGA ACAAAAACTC
     2341 ATCTCAGAAG AGGATCTGAA TGCTATGGGG CAACCCGGGA ACGGCAGCGC CTTCTTGCTG
     2401 GCACCCAATG GAAGCCATGC GCCGGACCAC GACGTCACGC AGCAAAGGGA CGAGGTGTGG
     2461 GTGGTGGGCA TGGGCATCGT CATGTCTCTC ATCGTCCTGG CCATCGTGTT TGGCAATGTG
     2521 CTGGTCATCA CAGCCATTGC CAAGTTCGAG CGTCTGCAGA CGGTCACCAA CTACTTCATC
     2581 ACTTCACTGG CCTGTGCTGA TCTGGTCATG GGCCTAGCAG TGGTGCCCTT TGGGGCCGCC
     2641 CATATTCTTA TGAAAATGTG GACTTTTGGC AACTTCTGGT GCGAGTTTTG GACTTCCATT
     2701 GATGTGCTGT GCGTCACGGC CAGCATTGAG ACCCTGTGCG TGATCGCAGT GGATCGCTAC
     2761 TTTGCCATTA CTTCACCTTT CAAGTACCAG AGCCTGCTGA CCAAGAATAA GGCCCGGGTG
     2821 ATCATTCTGA TGGTGTGGAT TGTGTCAGGC CTTACCTCCT TCTTGCCCAT TCAGATGCAC
     2881 TGGTACAGGG CCACCCACCA GGAAGCCATC AACTGCTATG CCAATGAGAC CTGCTGTGAC
     2941 TTCTTCACGA ACCAAGCCTA TGTCATTGCC TCTTCCATCG TGTCCTTCTA CGTTCCCCTG
     3001 GTGATCATGG TCTTCGTCTA CTCCAGGGTC TTTCAGGAGG CCAAAAGGCA GCTCCAGAAG
     3061 ATTGACAAAT CTGAGGGCCG CTTCCATGTC CAGAACCTTA GCCAGGTGGA GCAGGATGGG
     3121 CGGACGGGGC ATGGACTCCG CAGATCTTCC AAGTTCTGCT TGAAGGAGCA CAAAGCCCTC
     3181 AAGACGTTAG GCATCATCAT GGGCACTTTC ACCCTCTGCT GGCTGCCCTT CTTCATCGTT
     3241 AACATTGTGC ATGTGATCCA GGATAACCTC ATCCGTAAGG AAGTTTACAT CCTCCTAAAT
     3301 TGGATAGGCT ATGTCAATTC TGGTTTCAAT CCCCTTATCT ACTGCCGGAG CCCAGATTTC
     3361 AGGATTGCCT TCCAGGAGCT TCTGTGCCTG CGCAGGTCTT CTTTGAAGGC CTATGGGAAT
     3421 GGCTACTCCA GCAACGGCAA CACAGGGGAG CAGAGTGGAT ATCACGTGGA ACAGGAGAAA
     3481 GAAAATAAAC TGCTGTGTGA AGACCTCCCA GGCACGGAAG ACTTTGTGGG CCATCAAGGT
     3541 ACTGTGCCTA GCGATAACAT TGATTCACAA GGGAGGAATT GTAGTACAAA TGACTCACTG
     3601 CTGTAGaatg ctgtgggcca ggacacgcag gaggtcatcg tggtgccaca ctccttgccc
     3661 tttaaggtgg tggtgatctc agccatcctg gccctggtgg tgctcaccat catctccctt
     3721 atcatcctca tcatgctttg gcagaagaag ccacgtccac agcctgggtt agctcactca
     3781 ttaggcaccc caggctttac acttTATGCT TCCGGCTCGT ATGTTGTGTG AAATTGTGAG
     3841 CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGGATTCA CTGGCCGTCG
     3901 TTTTACAACG TCGTGactgg gaaaaccctg gcgttaccca anttaatcgc cttgcagcac
     3961 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     4021 agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc
     4081 cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact
     4141 ggcgttagcc agcaggtggt aggcggccgc tcgaccctgt ggaatgtgtg tcagttaggg
     4201 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4261 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4321 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     4381 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     4441 gccgaggccg cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     4501 ggcttttgca aaaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     4561 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     4621 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     4681 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     4741 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     4801 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     4861 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     4921 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     4981 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     5041 ctcggcttca ccgtcaccgc cgacgtcgag tgcccgaagg accgcgcgac ctggtgcatg
     5101 acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc
     5161 gcacgacccc atgcatcgat aaaataaaag attttattta gtctccagaa aaagggggga
     5221 atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca
     5281 tggaaaaata cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca
     5341 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     5401 agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     5461 cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta
     5521 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt
     5581 gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa
     5641 taaaagagcc cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg
     5701 gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc
     5761 ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttccgact
     5821 tgtggtctcg ctgccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt
     5881 cttcacatgc agcatgtatc aaaattaatt tggttttttt tcttaagtat ttacattaaa
     5941 tggccatagt tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggc
     6001 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6061 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6121 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6181 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6241 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6301 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6361 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     6421 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6481 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6541 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6601 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     6661 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6721 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6781 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6841 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     6901 tgcggccgca aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6961 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7021 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7081 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7141 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7201 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7261 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7321 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7381 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     7441 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     7501 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     7561 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     7621 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     7681 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     7741 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     7801 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     7861 atgagcggat acatatttga a   
//
