LOCUS       pBS_NL4_3_dEnv_eGFP_RT_D185KD186L       12624 bp ds-DNA     circular     16-AUG-2017
DEFINITION  pBluescript KS (-).
ACCESSION   urn.local...86-3xx88uo
VERSION     urn.local...86-3xx88uo
KEYWORDS    .
SOURCE      other sequences; artificial sequences; vectors
  ORGANISM  other sequences; artificial sequences; vectors. . .
            .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    1..454
                     /created_by="User"
                     /Transferred_From="5' long terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label="5' U3"
     primer_bind     complement(156..179)
                     /created_by="primer3"
                     /annotation_group="1485035306083655f715b75c-b114-4496-9283
                     -369339e6e911"
                     /%GC="54.2"
                     /Tm="62.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GGCCTCTTCTACTTGCTCTGGTTC"
                     /Transferred_From="5LTR_seq-R"
                     /Transferred_Similarity="100.00%"
                     /label="5LTR_seq-R"
     misc_feature    325..334
                     /created_by="User"
                     /Transferred_From="3' long terminal repeat, 5' long
                     terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label="RBEIII"
     misc_feature    349..359
                     /created_by="User"
                     /Transferred_From="3' long terminal repeat, 5' long
                     terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label="NF-kBii; NK-kBii"
     misc_feature    363..373
                     /created_by="User"
                     /Transferred_From="3' long terminal repeat, 5' long
                     terminal repeat"
                     /Transferred_Similarity="90.91%"
                     /label="NF-kBii; NK-kBii"
     misc_feature    377..386
                     /created_by="User"
                     /Transferred_From="5' long terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label="Ssp1iii"
     misc_feature    388..397
                     /created_by="User"
                     /Transferred_From="3' long terminal repeat, 5' long
                     terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label="Ssp1ii"
     misc_feature    399..408
                     /created_by="User"
                     /Transferred_From="3' long terminal repeat, 5' long
                     terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label="Ssp1i"
     misc_feature    455..551
                     /ApEinfo_fwdcolor="#a2ff71"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /Transferred_From="5' long terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label="5' R"
     misc_feature    455..512
                     /created_by="User"
                     /Transferred_From="3' long terminal repeat, 5' long
                     terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label="TAR"
     misc_feature    552..636
                     /created_by="User"
                     /Transferred_From="5' long terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label="5' U5"
     misc_feature    637..651
                     /Color="#ffddff"
                     /created_by="User"
                     /label="PBS"
     primer_bind     639..656
                     /created_by="primer3"
                     /annotation_group="1485044705485454991e48db-2eec-4c82-a2d3
                     -1c4ea55dca4a"
                     /%GC="66.7"
                     /Tm="60.7"
                     /Hairpin_Tm="65.5"
                     /Self_Dimer_Tm="2.3"
                     /Sequence="CGCCCGAACAGGGACTTG"
                     /Transferred_From="NL4-3_Pre-Gag_For"
                     /Transferred_Similarity="100.00%"
                     /label="NL4-3_Pre-Gag_For"
     misc_feature    686..823
                     /Color="#ffff55"
                     /created_by="User"
                     /label="Possible Psi Sequence"
     primer_bind     688..705
                     /created_by="primer3"
                     /annotation_group="1485044300815152d3da232a-d882-4e9b-8b3f
                     -0773771bb816"
                     /%GC="66.7"
                     /Tm="60.8"
                     /Hairpin_Tm="31.9"
                     /Self_Dimer_Tm="None"
                     /Sequence="GACGCAGGACTCGGCTTG"
                     /Transferred_From="NL4-3_Gag_For"
                     /Transferred_Similarity="100.00%"
                     /label="NL4-3_Gag_For"
     primer_bind     715..731
                     /created_by="primer3"
                     /annotation_group="1485043901522230ac164009-20cb-43dc-9213
                     -447e51533778"
                     /%GC="70.6"
                     /Tm="61.2"
                     /Hairpin_Tm="64.0"
                     /Self_Dimer_Tm="18.2"
                     /Sequence="GCACGGCAAGAGGCGAG"
                     /Transferred_From="NL4-3/8.9_Pre-Gag_For"
                     /Transferred_Similarity="100.00%"
                     /label="NL4-3/8.9_Pre-Gag_For"
     misc_feature    727..728
                     /Color="#bb33ff"
                     /created_by="User"
                     /label="D1b_novel"
     misc_feature    744..745
                     /created_by="User"
                     /Color="#cc00cc"
                     /modified_by="User"
                     /label="D1"
     motif           745..789
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="HIV-1_psi_pack"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="User"
                     /Transferred_From="HIV-1_psi_pack, Psi Pack signal"
                     /Transferred_Similarity="100.00%"
                     /label="HIV-1_psi_pack"
     misc_feature    748..749
                     /Color="#bb33ff"
                     /created_by="User"
                     /label="D1c_Cryptic"
     gene            790..2292
                     /modified_by="User"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Gag"
     misc_feature    790..1185
                     /created_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="MA p17"
     primer_bind     886..913
                     /created_by="primer3"
                     /annotation_group="148504457959915263b9ea3f-ff7d-487a-bdb1
                     -e6fc9e961968"
                     /%GC="50.0"
                     /Tm="64.4"
                     /Hairpin_Tm="41.8"
                     /Self_Dimer_Tm="None"
                     /Sequence="CATATAGTATGGGCAAGCAGGGAGCTAG"
                     /Transferred_From="NL4-3_MA_For"
                     /Transferred_Similarity="100.00%"
                     /label="NL4-3_MA_For"
     primer_bind     complement(898..917)
                     /created_by="primer3"
                     /annotation_group="1485047031246904d6dbeaa1-223d-4414-9be1
                     -db02e4cffca7"
                     /%GC="60.0"
                     /Tm="61.1"
                     /Hairpin_Tm="53.3"
                     /Self_Dimer_Tm="None"
                     /Sequence="CGTTCTAGCTCCCTGCTTGC"
                     /Transferred_From="psPAX2_CAG_MA_Rev"
                     /Transferred_Similarity="100.00%"
                     /label="psPAX2_CAG_MA_Rev"
     primer_bind     1156..1175
                     /created_by="primer3"
                     /annotation_group="1485034710494704d98eb6c4-e4ed-4844-9419
                     -ad2ae9adb1b8"
                     /%GC="55.0"
                     /Tm="59.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GGAAACAACAGCCAGGTCAG"
                     /Transferred_From="1156-1175_For"
                     /Transferred_Similarity="100.00%"
                     /label="1156-1175_For"
     misc_feature    1186..1878
                     /created_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="CA p24"
     primer_bind     1447..1465
                     /created_by="primer3"
                     /annotation_group="14850443316559972288cd58-000a-4a3c-bc26
                     -25915c72313b"
                     /%GC="63.2"
                     /Tm="61.1"
                     /Hairpin_Tm="48.3"
                     /Self_Dimer_Tm="18.3"
                     /Sequence="GCAGGGCCTATTGCACCAG"
                     /Transferred_From="NL4-3_Gag_For_2"
                     /Transferred_Similarity="100.00%"
                     /label="NL4-3_Gag_For_2"
     primer_bind     complement(1541..1565)
                     /created_by="primer3"
                     /annotation_group="14850404705169977b2d5b71-8eea-486d-9ba4
                     -97649f2c38b8"
                     /%GC="48.0"
                     /Tm="60.0"
                     /Hairpin_Tm="37.2"
                     /Self_Dimer_Tm="None"
                     /Sequence="CCTACTGGGATAGGTGGATTATGTG"
                     /Transferred_From="Gag_(CA)_751-776_Rev"
                     /Transferred_Similarity="100.00%"
                     /label="Gag_(CA)_751-776_Rev"
     primer_bind     1622..1641
                     /created_by="primer3"
                     /annotation_group="1485044269693189dbf87551-366e-4451-967b
                     -a68d7cb452aa"
                     /%GC="60.0"
                     /Tm="60.5"
                     /Hairpin_Tm="58.9"
                     /Self_Dimer_Tm="17.3"
                     /Sequence="GCCCTACCAGCATTCTGGAC"
                     /Transferred_From="NL4-3_Gag_833-852"
                     /Transferred_Similarity="100.00%"
                     /label="NL4-3_Gag_833-852"
     misc_feature    1879..1920
                     /created_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="p2"
     misc_feature    1921..2085
                     /created_by="User"
                     /modified_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="NC p7"
     gene            2085..5096
                     /modified_by="User"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Pol"
     misc_feature    2086..2133
                     /created_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="p1"
     misc_feature    2134..2289
                     /created_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="p6 Vpr binding"
     primer_bind     2144..2160
                     /created_by="primer3"
                     /annotation_group="1485044735747554291456ea-3b10-4d12-bc6e
                     -57d3014b35a8"
                     /%GC="64.7"
                     /Tm="57.5"
                     /Hairpin_Tm="45.5"
                     /Self_Dimer_Tm="None"
                     /Sequence="GACCAGAGCCAACAGCC"
                     /Transferred_From="NL4-3_Prot_For"
                     /Transferred_Similarity="100.00%"
                     /label="NL4-3_Prot_For"
     misc_feature    2253..2549
                     /created_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="PR p15"
     misc_feature    2325..2327
                     /created_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="D25A PR mutant"
     misc_feature    2326
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2550..3869
                     /created_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="RT p51"
     primer_bind     complement(2874..2890)
                     /created_by="primer3"
                     /annotation_group="14850447657182730894c8c4-5a81-4a92-bf04
                     -78b03d3b7100"
                     /%GC="64.7"
                     /Tm="58.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GCATCGCCCACATCCAG"
                     /Transferred_From="NL4-3_Prot_Rev"
                     /Transferred_Similarity="100.00%"
                     /label="NL4-3_Prot_Rev"
     primer_bind     2874..2890
                     /created_by="primer3"
                     /annotation_group="1485044673276056f2e2d3d1-6cef-4234-b859
                     -b7b1b2f12f2c"
                     /%GC="64.7"
                     /Tm="58.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CTGGATGTGGGCGATGC"
                     /Transferred_From="NL4-3_Pol_789-806_For"
                     /Transferred_Similarity="100.00%"
                     /label="NL4-3_Pol_789-806_For"
     misc_feature    3102..3107
                     /created_by="User"
                     /modified_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="D185K D186L RT mutant"
     primer_bind     3436..3462
                     /created_by="primer3"
                     /annotation_group="14850401645975503430f3a4-2181-43c5-9c3a
                     -a9c869d8cfce"
                     /%GC="51.9"
                     /Tm="65.2"
                     /Hairpin_Tm="38.3"
                     /Self_Dimer_Tm="14.2"
                     /Sequence="CAGAAGAAGCAGAGCTAGAACTGGCAG"
                     /Transferred_From="Fragment_2_For"
                     /Transferred_Similarity="100.00%"
                     /label="Fragment_2_For"
     primer_bind     3449..3473
                     /created_by="primer3"
                     /annotation_group="148504019560795992838a4d-f5c1-42cf-b521
                     -0854af0501fd"
                     /%GC="52.0"
                     /Tm="63.5"
                     /Hairpin_Tm="39.0"
                     /Self_Dimer_Tm="11.9"
                     /Sequence="GCTAGAACTGGCAGAAAACAGGGAG"
                     /Transferred_From="Fragment_2_For_inner"
                     /Transferred_Similarity="100.00%"
                     /label="Fragment_2_For_inner"
     primer_bind     3537..3553
                     /created_by="primer3"
                     /annotation_group="1485044609709092463933a6-a1ed-41ae-b308
                     -ade107505a16"
                     /%GC="64.7"
                     /Tm="57.5"
                     /Hairpin_Tm="44.6"
                     /Self_Dimer_Tm="None"
                     /Sequence="CAGAAGCAGGGGCAAGG"
                     /Transferred_From="NL4-3_Pol_1452-1469"
                     /Transferred_Similarity="100.00%"
                     /label="NL4-3_Pol_1452-1469"
     primer_bind     complement(3739..3759)
                     /created_by="primer3"
                     /annotation_group="1485040099585730d8f05b31-59fd-4268-94a0
                     -54eb84978e6a"
                     /%GC="57.1"
                     /Tm="61.3"
                     /Hairpin_Tm="48.7"
                     /Self_Dimer_Tm="None"
                     /Sequence="CTGTCCACCATGCTTCCCATG"
                     /Transferred_From="Fragment_1_inner_Rev"
                     /Transferred_Similarity="100.00%"
                     /label="Fragment_1_inner_Rev"
     primer_bind     complement(3751..3773)
                     /created_by="primer3"
                     /annotation_group="1485040133364033b6d90f51-dfe5-4b6d-bbc2
                     -1c39da9afb1e"
                     /%GC="56.5"
                     /Tm="63.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="6.8"
                     /Sequence="GGCTTGCCAATACTCTGTCCACC"
                     /Transferred_From="Fragment_1_Rev"
                     /Transferred_Similarity="100.00%"
                     /label="Fragment_1_Rev"
     misc_feature    3870..4229
                     /created_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="RNase H p15"
     misc_feature    4230..5093
                     /created_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="IN p31"
     primer_bind     4491..4512
                     /created_by="primer3"
                     /annotation_group="1485044640607499aca9c6ff-f75f-44a7-b5fe
                     -2d4eca6c686d"
                     /%GC="54.5"
                     /Tm="61.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GTAATTCCAGCAGAGACAGGGC"
                     /Transferred_From="NL4-3_Pol_2406-2428_For"
                     /Transferred_Similarity="100.00%"
                     /label="NL4-3_Pol_2406-2428_For"
     misc_feature    complement(4541..4542)
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label="A1a_Cryptic"
     primer_bind     complement(4549..4575)
                     /created_by="primer3"
                     /annotation_group="14850440560892336618cf55-39f2-4a83-8984
                     -b34a72c6fd21"
                     /%GC="40.7"
                     /Tm="60.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CTGTATGTACTGTTTTTACTGGCCATC"
                     /Transferred_From="NL4-3_D116A_ATW_Rev"
                     /Transferred_Similarity="100.00%"
                     /label="NL4-3_D116A_ATW_Rev"
     misc_feature    4575..4577
                     /created_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="D116A integrase mutant"
     misc_feature    4722..4723
                     /Color="#bb33ff"
                     /created_by="User"
                     /label="D1a_Cryptic"
     frag            4757..4934
                     /note="Charneau"
                     /label="Charneau"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="Charneau Sequence"
                     /Transferred_Similarity="100.00%"
     misc_signal     4785..4800
                     /note="cPPT"
                     /label="cPPT"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="Charneau Sequence, cPPT"
                     /Transferred_Similarity="100.00%"
     misc_signal     4864..4904
                     /note="Charneau Central Termination Signal"
                     /label="Charneau Central Termination Signal"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="Charneau Central Termination Signal,
                     Charneau Sequence"
                     /Transferred_Similarity="100.00%"
     primer_bind     4897..4915
                     /created_by="primer3"
                     /annotation_group="1485037745652715ba493877-d21e-472f-9305
                     -3644f0d6b403"
                     /%GC="42.1"
                     /Tm="53.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TTCGGGTTTATTACAGGGA"
                     /Transferred_From="cPPT_seq_F"
                     /Transferred_Similarity="100.00%"
                     /label="cPPT_seq_F"
     misc_feature    complement(4911..4912)
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label="A1"
     primer_bind     4913..4932
                     /created_by="primer3"
                     /annotation_group="1485046686868321450147b3-9267-4c76-a7d7
                     -e09f6cc0d595"
                     /%GC="50.0"
                     /Tm="57.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GGACAGCAGAGATCCAGTTT"
                     /Transferred_From="pol_III_seq_F1"
                     /Transferred_Similarity="100.00%"
                     /label="pol_III_seq_F1"
     misc_feature    4960..4970
                     /created_by="User"
                     /Color="#ff00ff"
                     /modified_by="User"
                     /label="U1 D2upEx"
     misc_feature    4963..4964
                     /created_by="User"
                     /Color="#aa00aa"
                     /modified_by="User"
                     /label="D2"
     misc_feature    5041..5619
                     /ApEinfo_fwdcolor="#f71cff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Vif"
     primer_bind     5195..5219
                     /created_by="primer3"
                     /annotation_group="148504486255351258b1c474-9c49-40cd-8563
                     -3ac1d1d77aad"
                     /%GC="48.0"
                     /Tm="60.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GTTCAGAAGTACACATCCCACTAGG"
                     /Transferred_From="NL4-3_Vif-5195_F"
                     /Transferred_Similarity="100.00%"
                     /label="NL4-3_Vif-5195_F"
     misc_feature    complement(5388..5389)
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label="A2"
     misc_feature    5422..5436
                     /created_by="User"
                     /Color="#00ff00"
                     /modified_by="User"
                     /label="ESSV (repress A2)"
     misc_feature    5462..5471
                     /Color="#ff00ff"
                     /created_by="User"
                     /label="U1 D3up"
     misc_feature    5464..5465
                     /created_by="User"
                     /Color="#bb33ff"
                     /modified_by="User"
                     /label="D3"
     primer_bind     5520..5541
                     /created_by="primer3"
                     /annotation_group="14850448935804313dec8804-1f31-4a5c-93b8
                     -11fb918cde8d"
                     /%GC="54.5"
                     /Tm="61.2"
                     /Hairpin_Tm="47.8"
                     /Self_Dimer_Tm="None"
                     /Sequence="GCCACCTTTGCCTAGTGTTAGG"
                     /Transferred_From="NL4-3_Vif-5520_F"
                     /Transferred_Similarity="100.00%"
                     /label="NL4-3_Vif-5520_F"
     misc_feature    5559..5849
                     /ApEinfo_fwdcolor="#cbcccf"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Vpr"
     misc_feature    complement(5775..5776)
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label="A3"
     misc_feature    5779..5785
                     /Color="#00ff00"
                     /created_by="User"
                     /label="ESS2p"
     misc_feature    5814..5824
                     /created_by="User"
                     /Color="#0000ff"
                     /modified_by="User"
                     /label="U1T5"
     misc_feature    join(5830..6044,8371..8416)
                     /ApEinfo_fwdcolor="#fff51f"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Tat"
     misc_feature    5834..5844
                     /created_by="User"
                     /Color="#0000ff"
                     /modified_by="User"
                     /label="U1T2"
     misc_feature    5838..5845
                     /Color="#00ff00"
                     /created_by="User"
                     /label="ESE2"
     primer_bind     complement(5839..5862)
                     /created_by="primer3"
                     /annotation_group="1485043957765855ec68604b-edbc-42ec-b374
                     -0903fb2b3885"
                     /%GC="54.2"
                     /Tm="60.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="0.1"
                     /Sequence="CCAGGGCTCTAGTCTAGGATCTAC"
                     /Transferred_From="NL4-3_Beg_Tat_Rev"
                     /Transferred_Similarity="100.00%"
                     /label="NL4-3_Beg_Tat_Rev"
     misc_feature    5846..5856
                     /created_by="User"
                     /Color="#0000ff"
                     /modified_by="User"
                     /label="U1T1"
     misc_feature    5846..5855
                     /created_by="User"
                     /Color="#00ff00"
                     /modified_by="User"
                     /label="ESS2 (repress tat)"
     misc_feature    5861..5871
                     /Color="#0000ff"
                     /created_by="User"
                     /label="U1T3"
     misc_feature    5876..5886
                     /Color="#0000ff"
                     /created_by="User"
                     /label="U1T4"
     misc_feature    5887..5897
                     /Color="#0000ff"
                     /created_by="User"
                     /label="U1T6"
     misc_feature    5906
                     /Color="#ff0000"
                     /created_by="User"
                     /label="BP1"
     misc_feature    5912
                     /Color="#ff0000"
                     /created_by="User"
                     /label="BP2"
     misc_feature    5927
                     /Color="#ff0000"
                     /created_by="User"
                     /label="BP3"
     misc_feature    5933
                     /Color="#ff0000"
                     /created_by="User"
                     /label="BP4"
     misc_feature    complement(5934..5935)
                     /Color="#ff9911"
                     /created_by="User"
                     /label="A4c"
     misc_feature    5944
                     /Color="#ff0000"
                     /created_by="User"
                     /label="BP5"
     misc_feature    5947
                     /created_by="User"
                     /Color="#ff0000"
                     /modified_by="User"
                     /label="BP6"
     misc_feature    complement(5952..5953)
                     /Color="#ff9911"
                     /created_by="User"
                     /label="A4a"
     misc_feature    5952
                     /Color="#ff0000"
                     /created_by="User"
                     /label="BP7"
     primer_bind     5953..5981
                     /created_by="primer3"
                     /annotation_group="1485043334665296ed69d491-8842-401c-917c
                     -20c19431f09a"
                     /%GC="51.7"
                     /Tm="67.6"
                     /Hairpin_Tm="60.5"
                     /Self_Dimer_Tm="26.7"
                     /Sequence="GCCTTAGGCATCTCCTATGGCAGGAAGAA"
                     /Transferred_From="MP_outer_Env_sense"
                     /Transferred_Similarity="100.00%"
                     /label="MP_outer_Env_sense"
     primer_bind     5954..5976
                     /created_by="primer3"
                     /annotation_group="14850403466083378dd935a6-4ec0-46ef-9dae
                     -10ccc8748d98"
                     /%GC="56.5"
                     /Tm="62.2"
                     /Hairpin_Tm="57.5"
                     /Self_Dimer_Tm="8.7"
                     /Sequence="CCTTAGGCATCTCCTATGGCAGG"
                     /Transferred_From="Fragment_3_For"
                     /Transferred_Similarity="100.00%"
                     /label="Fragment_3_For"
     misc_feature    complement(5958..5959)
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label="A4b"
     misc_feature    5958
                     /Color="#ff0000"
                     /created_by="User"
                     /label="BP8"
     primer_bind     5959..5983
                     /created_by="primer3"
                     /annotation_group="1485043223720178fc693e02-d299-46af-96b1
                     -743fa9a0f98f"
                     /%GC="56.0"
                     /Tm="65.2"
                     /Hairpin_Tm="53.3"
                     /Self_Dimer_Tm="10.8"
                     /Sequence="GGCATCTCCTATGGCAGGAAGAAGC"
                     /Transferred_From="MP_Env_sense"
                     /Transferred_Similarity="100.00%"
                     /label="MP_Env_sense"
     primer_bind     5959..5983
                     /created_by="primer3"
                     /annotation_group="14850399820718283a6ea8bf-fcb3-4106-b264
                     -7506066d2b00"
                     /%GC="56.0"
                     /Tm="65.2"
                     /Hairpin_Tm="53.3"
                     /Self_Dimer_Tm="10.8"
                     /Sequence="GGCATCTCCTATGGCAGGAAGAAGC"
                     /Transferred_From="Env_sense"
                     /Transferred_Similarity="100.00%"
                     /label="Env_sense"
     primer_bind     5966..5987
                     /created_by="primer3"
                     /annotation_group="14850403775808166e4c1dd2-5a1b-4869-afaf
                     -17955e0bee72"
                     /%GC="59.1"
                     /Tm="62.8"
                     /Hairpin_Tm="49.2"
                     /Self_Dimer_Tm="None"
                     /Sequence="CCTATGGCAGGAAGAAGCGGAG"
                     /Transferred_From="Fragment_3_For_inner"
                     /Transferred_Similarity="100.00%"
                     /label="Fragment_3_For_inner"
     misc_feature    join(5969..6044,8371..8644)
                     /ApEinfo_fwdcolor="#ec73ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Rev"
     misc_feature    complement(5974..5975)
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label="A5"
     misc_feature    5976..6001
                     /Color="#00ff00"
                     /created_by="User"
                     /label="GAR"
     misc_feature    complement(5978..5979)
                     /Color="#ff9911"
                     /created_by="User"
                     /label="A5a_Novel"
     misc_feature    complement(5981..5982)
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label="A5b_Novel"
     misc_feature    6045..6046
                     /created_by="User"
                     /Color="#cc00cc"
                     /modified_by="User"
                     /label="D4"
     misc_feature    6061..6306
                     /ApEinfo_fwdcolor="#5effab"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Vpu"
     primer_bind     6112..6133
                     /created_by="primer3"
                     /annotation_group="1485047092615349606e5b89-9860-4f71-b622
                     -b0792c438f89"
                     /%GC="45.5"
                     /Tm="57.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GCAATAGTTGTGTGGTCCATAG"
                     /Transferred_From="pSVIII_Env_For"
                     /Transferred_Similarity="100.00%"
                     /label="pSVIII_Env_For"
     primer_bind     6112..6133
                     /created_by="primer3"
                     /annotation_group="14850441736285693e3578c5-b76e-4507-8de3
                     -8641697ae054"
                     /%GC="45.5"
                     /Tm="57.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GCAATAGTTGTGTGGTCCATAG"
                     /Transferred_From="NL4-3_Env_For_(1)"
                     /Transferred_Similarity="100.00%"
                     /label="NL4-3_Env_For_(1)"
     sig_peptide     6221..6310
                     /vntifkey=94
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="SP"
     gene            6221..8787
                     /label="Env Polyprotein"
                     /ApEinfo_fwdcolor="#ffa729"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /Transferred_From="Env Polyprotein"
                     /Transferred_Similarity="99.84%"
     misc_feature    6311..7749
                     /created_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="gp120 Surface"
     primer_bind     6312..6344
                     /created_by="primer3"
                     /annotation_group="14850483658184943f0232c0-e1c5-4114-8306
                     -cc48a14a062c"
                     /%GC="45.5"
                     /Tm="67.2"
                     /Hairpin_Tm="39.3"
                     /Self_Dimer_Tm="None"
                     /Sequence="CAGAAAAATTGTGGGTCACCGTCTATTATGGGG"
                     /Transferred_From="Siliciano_add_BstEII_eGFP"
                     /Transferred_Similarity="100.00%"
                     /label="Siliciano_add_BstEII_eGFP"
     primer_bind     6322..6350
                     /created_by="primer3"
                     /annotation_group="1485047462844269aee9a55e-41a1-4f93-9313
                     -162188aa23af"
                     /%GC="55.2"
                     /Tm="67.5"
                     /Hairpin_Tm="45.8"
                     /Self_Dimer_Tm="5.6"
                     /Sequence="GTGGGTCACCGTCTATTATGGGGTACCTG"
                     /Transferred_From="Rons_BstEII_Env_For"
                     /Transferred_Similarity="100.00%"
                     /label="Rons_BstEII_Env_For"
     primer_bind     complement(6322..6347)
                     /created_by="primer3"
                     /annotation_group="148504028253848427106484-7fb1-4f55-92c8
                     -5486b8dec875"
                     /%GC="53.8"
                     /Tm="64.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GTACCCCATAATAGACGGTGACCCAC"
                     /Transferred_From="Fragment_2_Rev_inner"
                     /Transferred_Similarity="100.00%"
                     /label="Fragment_2_Rev_inner"
     misc_feature    6403..6404
                     /ApEinfo_fwdcolor="#00ff40"
                     /ApEinfo_revcolor="#80ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="NdeKill"
     primer_bind     6573..6601
                     /created_by="primer3"
                     /annotation_group="1485042565203248857869c1-c0e5-4f14-9904
                     -2fda48fa37e0"
                     /%GC="44.8"
                     /Tm="64.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GCCATGTGTAAAATTAACCCCACTCTGTG"
                     /Transferred_From="JRFL_(MfeI)_For"
                     /Transferred_Similarity="100.00%"
                     /label="JRFL_(MfeI)_For"
     misc_feature    complement(6604..6605)
                     /Color="#ff9911"
                     /created_by="User"
                     /label="A6_Cryptic"
     misc_feature    complement(6646..6647)
                     /Color="#ff9911"
                     /created_by="User"
                     /label="A6a_Novel"
     misc_feature    6727..6728
                     /Color="#bb33ff"
                     /created_by="User"
                     /label="D5_Cryptic"
     primer_bind     6856..6876
                     /created_by="primer3"
                     /annotation_group="1485044207137802fa4459d3-4e38-409a-8371
                     -09b2db325f29"
                     /%GC="52.4"
                     /Tm="59.1"
                     /Hairpin_Tm="36.5"
                     /Self_Dimer_Tm="None"
                     /Sequence="CCCATACATTATTGTGCCCCG"
                     /Transferred_From="NL4-3_Env_For_(2)"
                     /Transferred_Similarity="100.00%"
                     /label="NL4-3_Env_For_(2)"
     primer_bind     complement(6972..6996)
                     /created_by="primer3"
                     /annotation_group="1485044142907310090be42a-d15a-428f-8b4d
                     -a8c6b0836b7b"
                     /%GC="48.0"
                     /Tm="61.6"
                     /Hairpin_Tm="39.7"
                     /Self_Dimer_Tm="0.6"
                     /Sequence="CAGCAGTTGAGTTGATACTACTGGC"
                     /Transferred_From="NL4-3_Env_759-783_Rev"
                     /Transferred_Similarity="100.00%"
                     /label="NL4-3_Env_759-783_Rev"
     primer_bind     6972..6996
                     /created_by="primer3"
                     /annotation_group="14850445495070625b9e2af3-881d-40d1-bad6
                     -b98c7ad289db"
                     /%GC="48.0"
                     /Tm="61.6"
                     /Hairpin_Tm="39.5"
                     /Self_Dimer_Tm="11.4"
                     /Sequence="GCCAGTAGTATCAACTCAACTGCTG"
                     /Transferred_From="NL4-3_JRCSF_JRFL_Env_759-783_For"
                     /Transferred_Similarity="100.00%"
                     /label="NL4-3_JRCSF_JRFL_Env_759-783_For"
     primer_bind     7622..7641
                     /created_by="primer3"
                     /annotation_group="14850442385743496860a004-a8e3-4bc4-91ad
                     -d70875069f6d"
                     /%GC="60.0"
                     /Tm="59.4"
                     /Hairpin_Tm="47.6"
                     /Self_Dimer_Tm="None"
                     /Sequence="GACCTGGAGGAGGCGATATG"
                     /Transferred_From="NL4-3_Env_For_(3)"
                     /Transferred_Similarity="100.00%"
                     /label="NL4-3_Env_For_(3)"
     primer_bind     7690..7712
                     /created_by="primer3"
                     /annotation_group="148504448480000988588a07-e3fe-4fea-b5c6
                     -3f3e9713f9de"
                     /%GC="52.2"
                     /Tm="60.7"
                     /Hairpin_Tm="38.7"
                     /Self_Dimer_Tm="None"
                     /Sequence="GAACCATTAGGAGTAGCACCCAC"
                     /Transferred_From="NL4-3_JRCSF_JRFL_Env_1480-1502_For"
                     /Transferred_Similarity="100.00%"
                     /label="NL4-3_JRCSF_JRFL_Env_1480-1502_For"
     primer_bind     complement(7690..7712)
                     /created_by="primer3"
                     /annotation_group="1485044087435198fb32510d-44e0-4808-8893
                     -e582eb04b948"
                     /%GC="52.2"
                     /Tm="60.7"
                     /Hairpin_Tm="34.2"
                     /Self_Dimer_Tm="None"
                     /Sequence="GTGGGTGCTACTCCTAATGGTTC"
                     /Transferred_From="NL4-3_Env_1480-1502_Rev"
                     /Transferred_Similarity="100.00%"
                     /label="NL4-3_Env_1480-1502_Rev"
     misc_feature    7702..8053
                     /created_by="User"
                     /modified_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="RRE"
     misc_feature    7750..8784
                     /created_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="gp41 Transmembrane"
     misc_feature    8191..8265
                     /created_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="MPER sequence"
     misc_feature    8269..8328
                     /created_by="User"
                     /modified_by="User"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="TM domain"
     misc_feature    complement(8335..8336)
                     /Color="#ff9911"
                     /created_by="User"
                     /label="A7a_Cryptic"
     misc_feature    complement(8341..8342)
                     /Color="#ff9911"
                     /created_by="User"
                     /label="A7b_Cryptic"
     misc_feature    complement(8345..8346)
                     /Color="#ff9911"
                     /created_by="User"
                     /label="A7c_Novel"
     misc_feature    complement(8363..8364)
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label="A7d_Novel"
     misc_feature    complement(8369..8370)
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label="A7"
     misc_feature    complement(8382..8383)
                     /Color="#ff9911"
                     /created_by="User"
                     /label="A7e_Novel"
     misc_feature    8414..8424
                     /Color="#00ff00"
                     /created_by="User"
                     /label="(GAA)3"
     misc_feature    8426..8427
                     /Color="#bb33ff"
                     /created_by="User"
                     /label="D6_Cryptic"
     primer_bind     8428..8451
                     /created_by="primer3"
                     /annotation_group="1485044515990690c6712d7b-a9f1-4b9c-a81d
                     -f8db9feede72"
                     /%GC="54.2"
                     /Tm="61.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GGAGAGAGAGACAGAGACAGATCC"
                     /Transferred_From="NL4-3_JRCSF_JRFL_Env_2218-2241_For"
                     /Transferred_Similarity="100.00%"
                     /label="NL4-3_JRCSF_JRFL_Env_2218-2241_For"
     primer_bind     complement(8428..8451)
                     /created_by="primer3"
                     /annotation_group="14850399166218213b0cf531-e957-4ed3-98a4
                     -9d733094ca8a"
                     /%GC="54.2"
                     /Tm="61.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GGATCTGTCTCTGTCTCTCTCTCC"
                     /Transferred_From="Env_2218-2241_Rev"
                     /Transferred_Similarity="100.00%"
                     /label="Env_2218-2241_Rev"
     misc_feature    8446..8461
                     /created_by="User"
                     /Color="#00ff00"
                     /modified_by="User"
                     /label="ESS3 (repress A7)"
     misc_feature    complement(8486..8487)
                     /Color="#ff9911"
                     /created_by="User"
                     /label="A7f_Novel"
     misc_feature    complement(8515..8516)
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label="Possible_Acceptor"
     localization    8517..8546
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="HIV_Rev_NES loc"
                     /Transferred_Similarity="100.00%"
                     /label="HIV_Rev_NES loc"
     misc_feature    8654
                     /created_by="User"
                     /label="A->G"
     primer_bind     8683..8700
                     /created_by="primer3"
                     /annotation_group="148503855947558619175362-dff9-4e50-b268
                     -d29b419b5a3c"
                     /%GC="61.1"
                     /Tm="55.7"
                     /Hairpin_Tm="39.9"
                     /Self_Dimer_Tm="None"
                     /Sequence="GCTGAGGGGACAGATAGG"
                     /Transferred_From="dNef_sense"
                     /Transferred_Similarity="100.00%"
                     /label="dNef_sense"
     misc_feature    8789..8806
                     /ApEinfo_fwdcolor="#00ff40"
                     /ApEinfo_revcolor="#80ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="GFP start"
     misc_feature    8807..9526
                     /ApEinfo_fwdcolor="#00ff40"
                     /ApEinfo_revcolor="#80ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="GFP"
     primer_bind     complement(8987..9006)
                     /created_by="primer3"
                     /annotation_group="1485047375834763e96014e5-b9a7-4156-9a10
                     -5145103b46d7"
                     /%GC="60.0"
                     /Tm="60.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TAGGTCAGGGTGGTCACGAG"
                     /Transferred_From="reverse_for_upstream_of_eGFP-Vpr_LEI"
                     /Transferred_Similarity="100.00%"
                     /label="reverse_for_upstream_of_eGFP-Vpr_LEI"
     primer_bind     9399..9418
                     /created_by="primer3"
                     /annotation_group="14850398545197927929919a-3b50-42be-a353
                     -cf1837385607"
                     /%GC="55.0"
                     /Tm="59.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ACAACCACTACCTGAGCACC"
                     /Transferred_From="eGFP-Vpr_FW_on_GFP"
                     /Transferred_Similarity="100.00%"
                     /label="eGFP-Vpr_FW_on_GFP"
     primer_bind     9455..9475
                     /created_by="primer3"
                     /annotation_group="1485039823686087061f9c50-feb9-41ee-afbc
                     -d04912cf5467"
                     /%GC="57.1"
                     /Tm="60.5"
                     /Hairpin_Tm="44.2"
                     /Self_Dimer_Tm="12.0"
                     /Sequence="GATCACATGGTCCTGCTGGAG"
                     /Transferred_From="eGFP-Vpr_FW"
                     /Transferred_Similarity="100.00%"
                     /label="eGFP-Vpr_FW"
     primer_bind     9460..9481
                     /created_by="primer3"
                     /annotation_group="1485039793213818fe404410-19de-44bd-ab2e
                     -42d46ced5630"
                     /%GC="59.1"
                     /Tm="63.4"
                     /Hairpin_Tm="44.2"
                     /Self_Dimer_Tm="12.2"
                     /Sequence="CATGGTCCTGCTGGAGTTCGTG"
                     /Transferred_From="eGFP-C"
                     /Transferred_Similarity="100.00%"
                     /label="eGFP-C"
     gene            9533..10046
                     /created_by="User"
                     /ApEinfo_fwdcolor="#dc5aff"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Nef"
     primer_bind     complement(9542..9565)
                     /created_by="primer3"
                     /annotation_group="14850426575508664a0910a8-db2f-4f4f-aa71
                     -cb8fd171c1c6"
                     /%GC="41.7"
                     /Tm="61.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TTGCTACTTGTGATTGCTCCATGT"
                     /Transferred_From="Keele_outer_Env_antisense"
                     /Transferred_Similarity="100.00%"
                     /label="Keele_outer_Env_antisense"
     primer_bind     complement(9546..9570)
                     /created_by="primer3"
                     /annotation_group="1485043192698614b9f4c845-d5be-4b51-8ff6
                     -d0c79cee7d5b"
                     /%GC="44.0"
                     /Tm="60.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CTGTATTGCTACTTGTGATTGCTCC"
                     /Transferred_From="MP_Env_antisense"
                     /Transferred_Similarity="100.00%"
                     /label="MP_Env_antisense"
     primer_bind     complement(9546..9570)
                     /created_by="primer3"
                     /annotation_group="148503995029883296f950b2-c65e-4bc3-a456
                     -6c8844729823"
                     /%GC="44.0"
                     /Tm="60.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CTGTATTGCTACTTGTGATTGCTCC"
                     /Transferred_From="Env_antisense"
                     /Transferred_Similarity="100.00%"
                     /label="Env_antisense"
     primer_bind     complement(9645..9670)
                     /created_by="primer3"
                     /annotation_group="1485043279534540ac5c3873-af2e-4555-ae13
                     -3a1a48203a94"
                     /%GC="34.6"
                     /Tm="57.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="3.0"
                     /Sequence="TTGTAAGTCATTGGTCTTAAAGGTAC"
                     /Transferred_From="MP_outer_Env_antisense"
                     /Transferred_Similarity="100.00%"
                     /label="MP_outer_Env_antisense"
     primer_bind     complement(9710..9729)
                     /created_by="primer3"
                     /annotation_group="14850385286383106484028c-e80d-4ca8-b38c
                     -89ceaf6ecf42"
                     /%GC="55.0"
                     /Tm="57.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GAATTAGCCCTTCCAGTCCC"
                     /Transferred_From="dNef_antisense"
                     /Transferred_Similarity="100.00%"
                     /label="dNef_antisense"
     misc_feature    9715..10168
                     /created_by="User"
                     /Transferred_From="5' long terminal repeat"
                     /Transferred_Similarity="92.51%"
                     /label="5' U3"
     misc_feature    complement(9763..9764)
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label="A8d_Novel"
     misc_feature    complement(9779..9780)
                     /Color="#ff9911"
                     /created_by="User"
                     /label="A8a_Cryptic"
     misc_feature    complement(9791..9792)
                     /Color="#ff9911"
                     /created_by="User"
                     /label="A8c_Novel"
     misc_feature    complement(9799..9800)
                     /Color="#ff9911"
                     /created_by="User"
                     /label="A8_Cryptic"
     misc_feature    complement(9812..9813)
                     /Color="#ff9911"
                     /created_by="User"
                     /label="A8b_Cryptic"
     misc_feature    complement(9818..9819)
                     /Color="#ff9911"
                     /created_by="User"
                     /label="A8e_Novel"
     misc_feature    complement(9856..9857)
                     /Color="#ff9911"
                     /created_by="User"
                     /label="A8f_Novel"
     primer_bind     9944..9965
                     /created_by="primer3"
                     /annotation_group="1485043721767079437d3273-1f19-48aa-b564
                     -d7f283f939ed"
                     /%GC="54.5"
                     /Tm="59.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GGATGACCCTGAGAGAGAAGTG"
                     /Transferred_From="Nef-3LTR_seq-F"
                     /Transferred_Similarity="100.00%"
                     /label="Nef-3LTR_seq-F"
     primer_bind     complement(9969..9988)
                     /created_by="primer3"
                     /annotation_group="148503543263080695bf5cdb-813b-444c-8b9b
                     -0da76d2af3e6"
                     /%GC="60.0"
                     /Tm="61.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CGGCTGTCAAACCTCCACTC"
                     /Transferred_From="ALPS_zgreen_3-5"
                     /Transferred_Similarity="100.00%"
                     /label="ALPS_zgreen_3-5"
     primer_bind     complement(10034..10054)
                     /created_by="primer3"
                     /annotation_group="1485046057676878edd3ec8c-6318-4d71-8580
                     -24b689da385a"
                     /%GC="52.4"
                     /Tm="59.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="1.4"
                     /Sequence="GCTCGATGTCAGCAGTTCTTG"
                     /Transferred_From="pBS-ddNef-seq-R"
                     /Transferred_Similarity="100.00%"
                     /label="pBS-ddNef-seq-R"
     misc_feature    10039..10048
                     /created_by="User"
                     /Transferred_From="3' long terminal repeat, 5' long
                     terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label="RBEIII"
     misc_feature    10063..10073
                     /created_by="User"
                     /Transferred_From="3' long terminal repeat, 5' long
                     terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label="NF-kBii; NK-kBii"
     misc_feature    10077..10087
                     /created_by="User"
                     /Transferred_From="3' long terminal repeat, 5' long
                     terminal repeat"
                     /Transferred_Similarity="90.91%"
                     /label="NF-kBii; NK-kBii"
     misc_feature    10091..10100
                     /created_by="User"
                     /Transferred_From="3' long terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label="Sp1iii"
     misc_feature    10102..10111
                     /created_by="User"
                     /Transferred_From="3' long terminal repeat, 5' long
                     terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label="Ssp1ii"
     misc_feature    10113..10122
                     /created_by="User"
                     /Transferred_From="3' long terminal repeat, 5' long
                     terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label="Ssp1i"
     3'utr           10169..10265
                     /note="3' R"
                     /label="3' R"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="3' R"
                     /Transferred_Similarity="100.00%"
     misc_feature    10169..10226
                     /created_by="User"
                     /Transferred_From="3' long terminal repeat, 5' long
                     terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label="TAR"
     primer_bind     complement(10236..10265)
                     /created_by="primer3"
                     /annotation_group="1485042332117387097e9d96-5d95-4ec6-8a73
                     -58b34a6a79fb"
                     /%GC="46.7"
                     /Tm="67.7"
                     /Hairpin_Tm="33.3"
                     /Self_Dimer_Tm="28.2"
                     /Sequence="TGAAGCACTCAAGGCAAGCTTTATTGAGGC"
                     /Transferred_From="JFSG_inner_Env_antisense"
                     /Transferred_Similarity="100.00%"
                     /label="JFSG_inner_Env_antisense"
     primer_bind     complement(10240..10271)
                     /created_by="primer3"
                     /annotation_group="1485042388470782768532a9-2f2b-499c-85a7
                     -6b72d4b2eb66"
                     /%GC="40.6"
                     /Tm="65.9"
                     /Hairpin_Tm="57.3"
                     /Self_Dimer_Tm="20.8"
                     /Sequence="ACTACTTGAAGCACTCAAGGCAAGCTTTATTG"
                     /Transferred_From="JFSG_outer_Env_antisense"
                     /Transferred_Similarity="100.00%"
                     /label="JFSG_outer_Env_antisense"
     misc_feature    10241..10246
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="PolyA signal"
     3'utr           10266..10349
                     /note="3' U5"
                     /label="3' U5"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="3' U5"
                     /Transferred_Similarity="100.00%"
     rep_origin      complement(10406..11088)
                     /label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="ColE1 origin , ColE1 origin  rev"
                     /Transferred_Similarity="99.71%"
     primer_bind     complement(10411..10432)
                     /created_by="primer3"
                     /annotation_group="14850377143870693246930f-b8f9-4dea-b06b
                     -b1f356af1129"
                     /%GC="50.0"
                     /Tm="61.0"
                     /Hairpin_Tm="39.1"
                     /Self_Dimer_Tm="None"
                     /Sequence="CTATGGAAAAACGCCAGCAACG"
                     /Transferred_From="ColEI_Ori_Rev_pUC_mini"
                     /Transferred_Similarity="100.00%"
                     /label="ColEI_Ori_Rev_pUC_mini"
     primer_bind     complement(10464..10484)
                     /created_by="primer3"
                     /annotation_group="1485046087631088fba045de-724d-489d-9bbc
                     -5d383f9cf769"
                     /%GC="61.9"
                     /Tm="63.0"
                     /Hairpin_Tm="36.7"
                     /Self_Dimer_Tm="None"
                     /Sequence="CCACCTCTGACTTGAGCGTCG"
                     /Transferred_From="pBS-KS-R"
                     /Transferred_Similarity="100.00%"
                     /label="pBS-KS-R"
     gene            complement(11183..12043)
                     /gene="Ampicillin"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Ampicillin"
     promoter        complement(12085..12113)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AmpR_promoter"
     primer_bind     12132..12149
                     /created_by="primer3"
                     /annotation_group="14850471548177776777fd32-a2b2-4a72-9c0a
                     -c2a618284ee3"
                     /%GC="61.1"
                     /Tm="58.9"
                     /Hairpin_Tm="38.3"
                     /Self_Dimer_Tm="0.4"
                     /Sequence="CAAATAGGGGTTCCGCGC"
                     /Transferred_From="pUC_mini_For"
                     /Transferred_Similarity="100.00%"
                     /label="pUC_mini_For"
     rep_origin      12192..12498
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="f1_origin"
ORIGIN      
        1 tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
      121 tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca
      181 aataaggaga gaagaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg
      241 agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag
      301 agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg
      361 ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcggta ttaagcgggg gagaattaga taaatgggaa
      841 aaaattcggt taaggccagg gggaaagaaa caatataaac taaaacatat agtatgggca
      901 agcagggagc tagaacgatt cgcagttaat cctggccttt tagagacatc agaaggctgt
      961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1021 ttatataata caatagcagt cctctattgt gtgcatcaaa ggatagatgt aaaagacacc
     1081 aaggaagcct tagataagat agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa
     1141 gcagcagctg acacaggaaa caacagccag gtcagccaaa attaccctat agtgcagaac
     1201 ctccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaatgc atgggtaaaa
     1261 gtagtagaag agaaggcttt cagcccagaa gtaataccca tgttttcagc attatcagaa
     1321 ggagccaccc cacaagattt aaataccatg ctaaacacag tggggggaca tcaagcagcc
     1381 atgcaaatgt taaaagagac catcaatgag gaagctgcag aatgggatag attgcatcca
     1441 gtgcatgcag ggcctattgc accaggccag atgagagaac caaggggaag tgacatagca
     1501 ggaactacta gtacccttca ggaacaaata ggatggatga cacataatcc acctatccca
     1561 gtaggagaaa tctataaaag atggataatc ctgggattaa ataaaatagt aagaatgtat
     1621 agccctacca gcattctgga cataagacaa ggaccaaagg aaccctttag agactatgta
     1681 gaccgattct ataaaactct aagagccgag caagcttcac aagaggtaaa aaattggatg
     1741 acagaaacct tgttggtcca aaatgcgaac ccagattgta agactatttt aaaagcattg
     1801 ggaccaggag cgacactaga agaaatgatg acagcatgtc agggagtggg gggacccggc
     1861 cataaagcaa gagttttggc tgaagcaatg agccaagtaa caaatccagc taccataatg
     1921 atacagaaag gcaattttag gaaccaaaga aagactgtta agtgtttcaa ttgtggcaaa
     1981 gaagggcaca tagccaaaaa ttgcagggcc cctaggaaaa agggctgttg gaaatgtgga
     2041 aaggaaggac accaaatgaa agattgtact gagagacagg ctaatttttt agggaagatc
     2101 tggccttccc acaagggaag gccagggaat tttcttcaga gcagaccaga gccaacagcc
     2161 ccaccagaag agagcttcag gtttggggaa gagacaacaa ctccctctca gaagcaggag
     2221 ccgatagaca aggaactgta tcctttagct tccctcagat cactctttgg cagcgacccc
     2281 tcgtcacaat aaagataggg gggcaattaa aggaagctct attagctaca ggagcagatg
     2341 atacagtatt agaagaaatg aatttgccag gaagatggaa accaaaaatg atagggggaa
     2401 ttggaggttt tatcaaagta ggacagtatg atcagatact catagaaatc tgcggacata
     2461 aagctatagg tacagtatta gtaggaccta cacctgtcaa cataattgga agaaatctgt
     2521 tgactcagat tggctgcact ttaaattttc ccattagtcc tattgagact gtaccagtaa
     2581 aattaaagcc aggaatggat ggcccaaaag ttaaacaatg gccattgaca gaagaaaaaa
     2641 taaaagcatt agtagaaatt tgtacagaaa tggaaaagga aggaaaaatt tcaaaaattg
     2701 ggcctgaaaa tccatacaat actccagtat ttgccataaa gaaaaaagac agtactaaat
     2761 ggagaaaatt agtagatttc agagaactta ataagagaac tcaagatttc tgggaagttc
     2821 aattaggaat accacatcct gcagggttaa aacagaaaaa atcagtaaca gtactggatg
     2881 tgggcgatgc atatttttca gttcccttag ataaagactt caggaagtat actgcattta
     2941 ccatacctag tataaacaat gagacaccag ggattagata tcagtacaat gtgcttccac
     3001 agggatggaa aggatcacca gcaatattcc agtgtagcat gacaaaaatc ttagagcctt
     3061 ttagaaaaca aaatccagac atagtcatct atcaatacat ggatgatttg tatgtaggat
     3121 ctgacttaga aatagggcag catagaacaa aaatagagga actgagacaa catctgttga
     3181 ggtggggatt taccacacca gacaaaaaac atcagaaaga acctccattc ctttggatgg
     3241 gttatgaact ccatcctgat aaatggacag tacagcctat agtgctgcca gaaaaggaca
     3301 gctggactgt caatgacata cagaaattag tgggaaaatt gaattgggca agtcagattt
     3361 atgcagggat taaagtaagg caattatgta aacttcttag gggaaccaaa gcactaacag
     3421 aagtagtacc actaacagaa gaagcagagc tagaactggc agaaaacagg gagattctaa
     3481 aagaaccggt acatggagtg tattatgacc catcaaaaga cttaatagca gaaatacaga
     3541 agcaggggca aggccaatgg acatatcaaa tttatcaaga gccatttaaa aatctgaaaa
     3601 caggaaaata tgcaagaatg aagggtgccc acactaatga tgtgaaacaa ttaacagagg
     3661 cagtacaaaa aatagccaca gaaagcatag taatatgggg aaagactcct aaatttaaat
     3721 tacccataca aaaggaaaca tgggaagcat ggtggacaga gtattggcaa gccacctgga
     3781 ttcctgagtg ggagtttgtc aatacccctc ccttagtgaa gttatggtac cagttagaga
     3841 aagaacccat aataggagca gaaactttct atgtagatgg ggcagccaat agggaaacta
     3901 aattaggaaa agcaggatat gtaactgaca gaggaagaca aaaagttgtc cccctaacgg
     3961 acacaacaaa tcagaagact gagttacaag caattcatct agctttgcag gattcgggat
     4021 tagaagtaaa catagtgaca gactcacaat atgcattggg aatcattcaa gcacaaccag
     4081 ataagagtga atcagagtta gtcagtcaaa taatagagca gttaataaaa aaggaaaaag
     4141 tctacctggc atgggtacca gcacacaaag gaattggagg aaatgaacaa gtagatgggt
     4201 tggtcagtgc tggaatcagg aaagtactat ttttagatgg aatagataag gcccaagaag
     4261 aacatgagaa atatcacagt aattggagag caatggctag tgattttaac ctaccacctg
     4321 tagtagcaaa agaaatagta gccagctgtg ataaatgtca gctaaaaggg gaagccatgc
     4381 atggacaagt agactgtagc ccaggaatat ggcagctaga ttgtacacat ttagaaggaa
     4441 aagttatctt ggtagcagtt catgtagcca gtggatatat agaagcagaa gtaattccag
     4501 cagagacagg gcaagaaaca gcatacttcc tcttaaaatt agcaggaaga tggccagtaa
     4561 aaacagtaca tacagacaat ggcagcaatt tcaccagtac tacagttaag gccgcctgtt
     4621 ggtgggcggg gatcaagcag gaatttggca ttccctacaa tccccaaagt caaggagtaa
     4681 tagaatctat gaataaagaa ttaaagaaaa ttataggaca ggtaagagat caggctgaac
     4741 atcttaagac agcagtacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     4801 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     4861 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     4921 gagatccagt ttggaaagga ccagcaaagc tcctctggaa aggtgaaggg gcagtagtaa
     4981 tacaagataa tagtgacata aaagtagtgc caagaagaaa agcaaagatc atcagggatt
     5041 atggaaaaca gatggcaggt gatgattgtg tggcaagtag acaggatgag gattaacaca
     5101 tggaaaagat tagtaaaaca ccatatgtat atttcaagga aagctaagga ctggttttat
     5161 agacatcact atgaaagtac taatccaaaa ataagttcag aagtacacat cccactaggg
     5221 gatgctaaat tagtaataac aacatattgg ggtctgcata caggagaaag agactggcat
     5281 ttgggtcagg gagtctccat agaatggagg aaaaagagat atagcacaca agtagaccct
     5341 gacctagcag accaactaat tcatctgcac tattttgatt gtttttcaga atctgctata
     5401 agaaatacca tattaggacg tatagttagt cctaggtgtg aatatcaagc aggacataac
     5461 aaggtaggat ctctacagta cttggcacta gcagcattaa taaaaccaaa acagataaag
     5521 ccacctttgc ctagtgttag gaaactgaca gaggacagat ggaacaagcc ccagaagacc
     5581 aagggccaca gagggagcca tacaatgaat ggacactaga gcttttagag gaacttaaga
     5641 gtgaagctgt tagacatttt cctaggatat ggctccataa cttaggacaa catatctatg
     5701 aaacttacgg ggatacttgg gcaggagtgg aagccataat aagaattctg caacaactgc
     5761 tgtttatcca tttcagaatt gggtgtcgac atagcagaat aggcgttact cgacagagga
     5821 gagcaagaaa tggagccagt agatcctaga ctagagccct ggaagcatcc aggaagtcag
     5881 cctaaaactg cttgtaccaa ttgctattgt aaaaagtgtt gctttcattg ccaagtttgt
     5941 ttcatgacaa aagccttagg catctcctat ggcaggaaga agcggagaca gcgacgaaga
     6001 gctcatcaga acagtcagac tcatcaagct tctctatcaa agcagtaagt agtacatgta
     6061 atgcaaccta taatagtagc aatagtagca ttagtagtag caataataat agcaatagtt
     6121 gtgtggtcca tagtaatcat agaatatagg aaaatattaa gacaaagaaa aatagacagg
     6181 ttaattgata gactaataga aagagcagaa gacagtggca atgagagtga aggagaagta
     6241 tcagcacttg tggagatggg ggtggaaatg gggcaccatg ctccttggga tattgatgat
     6301 ctgtagtgct acagaaaaat tgtgggtcac cgtctattat ggggtacctg tgtggaagga
     6361 agcaaccacc actctatttt gtgcatcaga tgctaaagca tatatgatac agaggtacat
     6421 aatgtttggg ccacacatgc ctgtgtaccc acagacccca acccacaaga agtagtattg
     6481 gtaaatgtga cagaaaattt taacatgtgg aaaaatgaca tggtagaaca gatgcatgag
     6541 gatataatca gtttatggga tcaaagccta aagccatgtg taaaattaac cccactctgt
     6601 gttagtttaa agtgcactga tttgaagaat gatactaata ccaatagtag tagcgggaga
     6661 atgataatgg agaaaggaga gataaaaaac tgctctttca atatcagcac aagcataaga
     6721 gataaggtgc agaaagaata tgcattcttt tataaacttg atatagtacc aatagataat
     6781 accagctata ggttgataag ttgtaacacc tcagtcatta cacaggcctg tccaaaggta
     6841 tcctttgagc caattcccat acattattgt gccccggctg gttttgcgat tctaaaatgt
     6901 aataataaga cgttcaatgg aacaggacca tgtacaaatg tcagcacagt acaatgtaca
     6961 catggaatca ggccagtagt atcaactcaa ctgctgttaa atggcagtct agcagaagaa
     7021 gatgtagtaa ttagatctgc caatttcaca gacaatgcta aaaccataat agtacagctg
     7081 aacacatctg tagaaattaa ttgtacaaga cccaacaaca atacaagaaa aagtatccgt
     7141 atccagaggg gaccagggag agcatttgtt acaataggaa aaataggaaa tatgagacaa
     7201 gcacattgta acattagtag agcaaaatgg aatgccactt taaaacagat agctagcaaa
     7261 ttaagagaac aatttggaaa taataaaaca ataatcttta agcaatcctc aggaggggac
     7321 ccagaaattg taacgcacag ttttaattgt ggaggggaat ttttctactg taattcaaca
     7381 caactgttta atagtacttg gtttaatagt acttggagta ctgaagggtc aaataacact
     7441 gaaggaagtg acacaatcac actcccatgc agaataaaac aatttataaa catgtggcag
     7501 gaagtaggaa aagcaatgta tgcccctccc atcagtggac aaattagatg ttcatcaaat
     7561 attactgggc tgctattaac aagagatggt ggtaataaca acaatgggtc cgagatcttc
     7621 agacctggag gaggcgatat gagggacaat tggagaagtg aattatataa atataaagta
     7681 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     7741 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     7801 actatgggcg cacggtcaat gacgctgacg gtacaggcca gacaattatt gtctgatata
     7861 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     7921 acagtctggg gcatcaaaca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     7981 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     8041 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaataa catgacctgg
     8101 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     8161 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     8221 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     8281 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     8341 aggcagggat attcaccatt atcgtttcag acccacctcc caatcccgag gggacccgac
     8401 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     8461 gtgaacggat ccttagcact tatctgggac gatctgcgga gcctgtgcct cttcagctac
     8521 caccgcttga gagacttact cttgattgta acgaggattg tggaacttct gggacgcagg
     8581 gggtgggaag ccctcaaata ttggtggaat ctcctacagt attggagtca ggaactaaag
     8641 aatagtgctg ttagcttgct caatgccaca gccatagcag tagctgaggg gacagatagg
     8701 gttatagaag tattacaagc agcttataga gctattcgcc acatacctag aagaataaga
     8761 cagggcttgg aaaggatttt gctataagat gggtggcgcg gccgctctgg tgagcaaggg
     8821 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     8881 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     8941 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     9001 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     9061 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     9121 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     9181 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     9241 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     9301 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     9361 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     9421 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     9481 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaactcg agcctagaaa
     9541 aacatggagc aatcacaagt agcaatacag cagctaacaa tgctgcttgt gcctggctag
     9601 aagcacaaga ggaggaagag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     9661 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     9721 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
     9781 gctacttccc tgattggcag aactacacac cagggccagg ggtcagatat ccactgacct
     9841 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     9901 gagagaacac cagcttgtta caccctgtga gcctgcatgg aatggatgac cctgagagag
     9961 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
    10021 atccggagta cttcaagaac tgctgacatc gagcttgcta caagggactt tccgctgggg
    10081 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatgctgca
    10141 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
    10201 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
    10261 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
    10321 ttttagtcag tgtggaaaat ctctagcagg ccggcccaat tgacatgtga gcaaaaggcc
    10381 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    10441 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    10501 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    10561 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    10621 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    10681 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    10741 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    10801 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    10861 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    10921 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    10981 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    11041 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    11101 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    11161 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    11221 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    11281 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    11341 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    11401 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    11461 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    11521 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    11581 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    11641 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    11701 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    11761 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    11821 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    11881 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    11941 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    12001 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    12061 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    12121 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc
    12181 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
    12241 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
    12301 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
    12361 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
    12421 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
    12481 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
    12541 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
    12601 attttaacaa aatattttaa ttaa
//
