LOCUS       pALPS_full_3__LT        6773 bp ds-DNA     circular     23-NOV-2015
DEFINITION  -.
ACCESSION   - (modified)
KEYWORDS    -.
SOURCE      null
  ORGANISM   .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     rep_origin      236..579
                     /note="SV40 ori/prom"
                     /label=SV40 ori/prom
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        580..934
                     /note="CMVieProm"
                     /label=CMVieProm
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2244..2248
                     /note="gag"
                     /modified_by="User"
                     /label=gag
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2249..3104
                     /note="env sequence"
                     /modified_by="User"
                     /label=env sequence
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     2392..2631
                     /note="RRE"
                     /label=RRE
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     3001..3005
                     /note="3' SA"
                     /label=3' SA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     frag            3130..3307
                     /note="Charneau"
                     /label=Charneau
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     3158..3173
                     /note="cPPT"
                     /label=cPPT
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     3237..3277
                     /note="Charneau Central Termination Signal"
                     /label=Charneau Central Termination Signal
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     3283..3287
                     /note="3'SA"
                     /label=3'SA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        3316..3817
                     /note="SFFVprom"
                     /label=SFFVprom
                     /ApEinfo_fwdcolor=#008080
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     3719..3738
                     /Sequence="CGCTTCTGTTCGCGCGCTTC"
                     /Hairpin_Tm="43.0"
                     /Tm="65.4"
                     /Self_Dimer_Tm="18.6"
                     /%GC="65.0"
                     /created_by="primer3"
                     /label=pscALPS for
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     oriT            4962..5632
                     /note="colE1"
                     /label=colE1
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(5723..6623)
                     /note="Ampicillin resistance"
                     /label=Ampicillin resistance
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     marker          complement(5780..6640)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp marker"
                     /Transferred_Similarity="99.88%"
                     /label=amp marker
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4255..4955
                     /label=LTR full
                     /ApEinfo_fwdcolor=#00ff40
                     /ApEinfo_revcolor=#80ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    935..2243
                     /label=GFP at start
                     /ApEinfo_fwdcolor=#00ff40
                     /ApEinfo_revcolor=#80ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3844..4221
                     /label=Rev
                     /ApEinfo_fwdcolor=#00ff40
                     /ApEinfo_revcolor=#80ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4222..4254
                     /label=WCHVrid
                     /ApEinfo_fwdcolor=#00ff40
                     /ApEinfo_revcolor=#80ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgctgtg
      241 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
      301 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
      361 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
      421 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
      481 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
      541 aggaggcttt tttggaggcc taggcttttg caaaaagctt tgacattgat tattgactag
      601 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      661 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      721 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      781 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      841 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      901 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      961 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     1021 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     1081 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     1141 gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga
     1201 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     1261 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     1321 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     1381 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     1441 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     1501 ctagaaggag agagatggtg tcaaaaggag aagaactgtt caccggagtg gtcccaatcc
     1561 tggtggagct ggacggagat gtcaatggcc acaagttcag cgtgagcgga gagggagaag
     1621 gagacgctac atacggcaag ctgactctga aattcatttg caccacaggg aagctgcccg
     1681 tgccttggcc aaccctggtc actaccctga catacggcgt ccagtgtttt agcagatatc
     1741 cagaccacat gaagcagcat gatttcttta aatccgccat gcccgagggc tacgtgcagg
     1801 aacggacaat cttctttaag gacgatggaa actataaaac tagagctgag gtgaagttcg
     1861 aaggagacac cctggtcaac aggatcgagc tgaagggcat tgactttaaa gaagatggga
     1921 atattctggg acacaagctg gagtacaact acaactctca taacgtgtat atcatggcag
     1981 ataagcagaa aaacggcatt aaggtcaatt tcaaaatccg ccacaatatt gaagacggga
     2041 gcgtgcagct ggccgatcat taccagcaga acacacctat cggcgacgga cccgtgctgc
     2101 tgcctgataa tcactatctg tctactcaga gtgccctgtc aaaggaccca aacgagaaac
     2161 gagatcatat ggtgctgctg gagtttgtca ccgctgctgg aatcacactg ggcatggatg
     2221 agctgtacaa gtaacggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     2281 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     2341 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     2401 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     2461 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     2521 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     2581 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     2641 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     2701 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2761 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2821 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2881 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2941 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     3001 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     3061 ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca
     3121 attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     3181 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     3241 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     3301 agtttggtta attaactgca gccccgataa aataaaagat tttatttagt ctccagaaaa
     3361 aggggggaat gaaagacccc acctgtaggt ttggcaagct agctgcagta acgccatttt
     3421 gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg gcgggtacat
     3481 gaaaatagct aacgttgggc caaacaggat atctgcggtg agcagtttcg gccccggccc
     3541 ggggccaaga acagatggtc accgcagttt cggccccggc ccgaggccaa gaacagatgg
     3601 tccccagata tggcccaacc ctcagcagtt tcttaagacc catcagatgt ttccaggctc
     3661 ccccaaggac ctgaaatgac cctgcgcctt atttgaatta accaatcagc ctgcttctcg
     3721 cttctgttcg cgcgcttctg cttcccgagc tctataaaag agctcacaac ccctcactcg
     3781 gcgcgccagt cctccgacag actgagtcgc ccgggggtct agaagcgctg gatccgttta
     3841 aacgcggccg ccacgcgtgc caccatggcc ggaagatctg gcgacagtga tgaggaactg
     3901 atccgaacag tgaggctgat taagctgctg taccagagca acccccctcc aaatcccgag
     3961 ggcactaggc aggcacgaag aaacaggcgc cgacggtgga gagagaggca gcgacagatc
     4021 cacagcattt ccgaacgcat cctgtcaacc tatctgggcc ggagcgccga gccagtgcca
     4081 ctgcagctgc cccctctgga gcggctgaca ctggactgca atgaagattg tgggaccagt
     4141 ggaacacagg gcgtcgggtc ccctcagatt ctggtggaat ccccaactgt cctggagtct
     4201 ggcaccaaag aatgacccgg ggcgatcgca gacctgcagg tggcctcagg tacctttaag
     4261 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     4321 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     4381 acaaggctac ttccctgatt ggcagaacta cacaccaggg ccaggggtca gatatccact
     4441 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     4501 taaaggagag aacaccagct tgttacaccc tgtgagcctg catggaatgg atgaccctga
     4561 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     4621 gctgcatccg gagtacttca agaactgctg acatcgagct tgctacaagg gactttccgc
     4681 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatg
     4741 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     4801 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     4861 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     4921 gaccctttta gtcagtgtgg aaaatctcta gcaggcgttt catgtgagca aaaggccagc
     4981 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     5041 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     5101 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5161 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     5221 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5281 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5341 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5401 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5461 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5521 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5581 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5641 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     5701 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     5761 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     5821 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     5881 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     5941 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6001 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6061 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     6121 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6181 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6241 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6301 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6361 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6421 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6481 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6541 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6601 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6661 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     6721 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gac
//
