LOCUS       pBS_Gab_BstEII          8864 bp ds-DNA     circular     15-JUN-2017
DEFINITION  pBluescript KS (-).
ACCESSION   urn.local...86-3xx88uo
VERSION     urn.local...86-3xx88uo
KEYWORDS    .
SOURCE      other sequences; artificial sequences; vectors
  ORGANISM  other sequences; artificial sequences; vectors. . . .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     rep_origin      24..330
                     /label=f1_origin
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    443..448
                     /created_by="User"
                     /label=SspI
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    449..456
                     /created_by="User"
                     /label=PacI
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    457..910
                     /created_by="User"
                     /Transferred_From="5' long terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label=5' U3
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    781..790
                     /created_by="User"
                     /Transferred_From="3' long terminal repeat, 5' long
                     terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label=RBEIII
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    805..815
                     /created_by="User"
                     /Transferred_From="3' long terminal repeat, 5' long
                     terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label=NF-kBii; NK-kBii
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    819..829
                     /created_by="User"
                     /Transferred_From="3' long terminal repeat, 5' long
                     terminal repeat"
                     /Transferred_Similarity="90.91%"
                     /label=NF-kBii; NK-kBii(1)
                     /ApEinfo_label="NF-kBii; NK-kBii"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    833..842
                     /created_by="User"
                     /Transferred_From="5' long terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label=Ssp1iii
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    844..853
                     /created_by="User"
                     /Transferred_From="3' long terminal repeat, 5' long
                     terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label=Ssp1ii
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    855..864
                     /created_by="User"
                     /Transferred_From="3' long terminal repeat, 5' long
                     terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label=Ssp1i
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    911..1007
                     /modified_by="User"
                     /Transferred_From="5' long terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label=5' R
                     /ApEinfo_fwdcolor="#a2ff71"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1008..1092
                     /created_by="User"
                     /Transferred_From="5' long terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label=5' U5
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            1253..1792
                     /modified_by="User"
                     /label=Pol
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1253..1789
                     /created_by="User"
                     /label=IN p31
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1271..1273
                     /created_by="User"
                     /label=D116A integrase mutant
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1418..1419
                     /Color="#bb33ff"
                     /created_by="User"
                     /label=D1a_Cryptic
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_signal     1481..1496
                     /note="cPPT"
                     /Transferred_From="Charneau Sequence, cPPT"
                     /Transferred_Similarity="100.00%"
                     /label=cPPT
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_signal     1560..1600
                     /note="Charneau Central Termination Signal"
                     /Transferred_From="Charneau Central Termination Signal,
                     Charneau Sequence"
                     /Transferred_Similarity="100.00%"
                     /label=Charneau Central Termination Signal
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1607..1608)
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label=A1
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1659..1660
                     /created_by="User"
                     /Color="#aa00aa"
                     /modified_by="User"
                     /label=D2
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1737..2315
                     /label=Vif
                     /ApEinfo_fwdcolor="#f71cff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2084..2085)
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label=A2
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2160..2161
                     /created_by="User"
                     /Color="#bb33ff"
                     /modified_by="User"
                     /label=D3
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2255..2545
                     /label=Vpr
                     /ApEinfo_fwdcolor="#cbcccf"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2472..2473)
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label=A3
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(2526..2740,5067..5112)
                     /label=Tat
                     /ApEinfo_fwdcolor="#fff51f"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2630..2631)
                     /Color="#ff9911"
                     /created_by="User"
                     /label=A4c
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2648..2649)
                     /Color="#ff9911"
                     /created_by="User"
                     /label=A4a
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2654..2655)
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label=A4b
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(2665..2740,5067..5340)
                     /label=Rev
                     /ApEinfo_fwdcolor="#ec73ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2670..2671)
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label=A5
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2674..2675)
                     /Color="#ff9911"
                     /created_by="User"
                     /label=A5a_Novel
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2677..2678)
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label=A5b_Novel
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2741..2742
                     /created_by="User"
                     /Color="#cc00cc"
                     /modified_by="User"
                     /label=D4
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2757..3002
                     /label=Vpu
                     /ApEinfo_fwdcolor="#5effab"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     sig_peptide     2917..3006
                     /vntifkey=94
                     /label=SP
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3007..4445
                     /created_by="User"
                     /label=gp120 Surface
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3099..3100
                     /label=NdeKill
                     /ApEinfo_fwdcolor="#00ff40"
                     /ApEinfo_revcolor="#80ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(3300..3301)
                     /Color="#ff9911"
                     /created_by="User"
                     /label=A6_Cryptic
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(3342..3343)
                     /Color="#ff9911"
                     /created_by="User"
                     /label=A6a_Novel
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3423..3424
                     /Color="#bb33ff"
                     /created_by="User"
                     /label=D5_Cryptic
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4398..4749
                     /created_by="User"
                     /modified_by="User"
                     /label=RRE
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4446..5480
                     /created_by="User"
                     /label=gp41 Transmembrane
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4887..4961
                     /created_by="User"
                     /label=MPER sequence
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4965..5024
                     /created_by="User"
                     /modified_by="User"
                     /label=TM domain
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5031..5032)
                     /Color="#ff9911"
                     /created_by="User"
                     /label=A7a_Cryptic
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5037..5038)
                     /Color="#ff9911"
                     /created_by="User"
                     /label=A7b_Cryptic
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5041..5042)
                     /Color="#ff9911"
                     /created_by="User"
                     /label=A7c_Novel
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5059..5060)
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label=A7d_Novel
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5065..5066)
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label=A7
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5078..5079)
                     /Color="#ff9911"
                     /created_by="User"
                     /label=A7e_Novel
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5122..5123
                     /Color="#bb33ff"
                     /created_by="User"
                     /label=D6_Cryptic
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5182..5183)
                     /Color="#ff9911"
                     /created_by="User"
                     /label=A7f_Novel
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5211..5212)
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label=Possible_Acceptor
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     localization    5213..5242
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="HIV_Rev_NES loc"
                     /Transferred_Similarity="100.00%"
                     /label=HIV_Rev_NES loc
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5350..5350
                     /Original_Bases="A"
                     /note="Geneious type: Editing History Replacement"
                     /label=A
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5350..5350
                     /created_by="User"
                     /label=A->G
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5485..5502
                     /label=GFP start
                     /ApEinfo_fwdcolor="#00ff40"
                     /ApEinfo_revcolor="#80ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5494..5501
                     /label=Not1
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#80ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5503..6222
                     /label=GFP
                     /ApEinfo_fwdcolor="#00ff40"
                     /ApEinfo_revcolor="#80ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            6229..6742
                     /created_by="User"
                     /label=Nef
                     /ApEinfo_fwdcolor="#dc5aff"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6411..6864
                     /created_by="User"
                     /Transferred_From="5' long terminal repeat"
                     /Transferred_Similarity="92.51%"
                     /label=5' U3(1)
                     /ApEinfo_label="5' U3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6459..6460)
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label=A8d_Novel
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6475..6476)
                     /Color="#ff9911"
                     /created_by="User"
                     /label=A8a_Cryptic
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6487..6488)
                     /Color="#ff9911"
                     /created_by="User"
                     /label=A8c_Novel
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6495..6496)
                     /Color="#ff9911"
                     /created_by="User"
                     /label=A8_Cryptic
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6508..6509)
                     /Color="#ff9911"
                     /created_by="User"
                     /label=A8b_Cryptic
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6514..6515)
                     /Color="#ff9911"
                     /created_by="User"
                     /label=A8e_Novel
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6552..6553)
                     /Color="#ff9911"
                     /created_by="User"
                     /label=A8f_Novel
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6735..6744
                     /created_by="User"
                     /Transferred_From="3' long terminal repeat, 5' long
                     terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label=RBEIII(1)
                     /ApEinfo_label="RBEIII"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6759..6769
                     /created_by="User"
                     /Transferred_From="3' long terminal repeat, 5' long
                     terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label=NF-kBii; NK-kBii(2)
                     /ApEinfo_label="NF-kBii; NK-kBii"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6773..6783
                     /created_by="User"
                     /Transferred_From="3' long terminal repeat, 5' long
                     terminal repeat"
                     /Transferred_Similarity="90.91%"
                     /label=NF-kBii; NK-kBii(3)
                     /ApEinfo_label="NF-kBii; NK-kBii"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6787..6796
                     /created_by="User"
                     /Transferred_From="3' long terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label=Sp1iii
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6798..6807
                     /created_by="User"
                     /Transferred_From="3' long terminal repeat, 5' long
                     terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label=Ssp1ii(1)
                     /ApEinfo_label="Ssp1ii"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6809..6818
                     /created_by="User"
                     /Transferred_From="3' long terminal repeat, 5' long
                     terminal repeat"
                     /Transferred_Similarity="100.00%"
                     /label=Ssp1i(1)
                     /ApEinfo_label="Ssp1i"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'utr           6865..6961
                     /note="3' R"
                     /Transferred_From="3' R"
                     /Transferred_Similarity="100.00%"
                     /label=3' R
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6937..6942
                     /label=PolyA signal
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'utr           6962..7045
                     /note="3' U5"
                     /Transferred_From="3' U5"
                     /Transferred_Similarity="100.00%"
                     /label=3' U5
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7045..7052
                     /created_by="User"
                     /label=FseI
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7059..7064
                     /created_by="User"
                     /label=AflIII
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(7102..7784)
                     /Transferred_From="ColE1 origin , ColE1 origin  rev"
                     /Transferred_Similarity="99.71%"
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(7879..8739)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label=ORF frame 3
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            complement(7879..8739)
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(8781..8809)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label=AmpR_promoter
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      421 aatttaacgc gaattttaac aaaatatttt aattaatgga agggctaatt tggtcccaaa
      481 aaagacaaga gatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
      541 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgcttcaagt
      601 tagtaccagt tgaaccagag caagtagaag aggccaaata aggagagaag aacagcttgt
      661 tacaccctat gagccagcat gggatggagg acccggaggg agaagtatta gtgtggaagt
      721 ttgacagcct cctagcattt cgtcacatgg cccgagagct gcatccggag tactacaaag
      781 actgctgaca tcgagctttc tacaagggac tttccgctgg ggactttcca gggaggtgtg
      841 gcctgggcgg gactggggag tggcgagccc tcagatgcta catataagca gctgcttttt
      901 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
      961 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgctcaaagt agtgtgtgcc
     1021 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     1081 atctctagca gtGGCGCCCG AACAGGGACT TGAAAGCGAA AGTAAAGCCA GAGGAGATCT
     1141 CTCGACGCAG GACTCGGCTT GCTGAAGCGC GCACGGCAAG AGGCGAGGGG CGGCGACTGG
     1201 TGAGTACGCC AAAAATTTTG ACTAGCGGAG GCTAGAAGGA GAGAGATGGC CAgtaaaaac
     1261 agtacataca gacaatggca gcaatttcac cagtactaca gttaaggccg cctgttggtg
     1321 ggcggggatc aagcaggaat ttggcattcc ctacaatccc caaagtcaag gagtaataga
     1381 atctatgaat aaagaattaa agaaaattat aggacaggta agagatcagg ctgaacatct
     1441 taagacagca gtacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     1501 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     1561 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     1621 tccagtttgg aaaggaccag caaagctcct ctggaaaggt gaaggggcag tagtaataca
     1681 agataatagt gacataaaag tagtgccaag aagaaaagca aagatcatca gggattatgg
     1741 aaaacagatg gcaggtgatg attgtgtggc aagtagacag gatgaggatt aacacatgga
     1801 aaagattagt aaaacaccat atgtatattt caaggaaagc taaggactgg ttttatagac
     1861 atcactatga aagtactaat ccaaaaataa gttcagaagt acacatccca ctaggggatg
     1921 ctaaattagt aataacaaca tattggggtc tgcatacagg agaaagagac tggcatttgg
     1981 gtcagggagt ctccatagaa tggaggaaaa agagatatag cacacaagta gaccctgacc
     2041 tagcagacca actaattcat ctgcactatt ttgattgttt ttcagaatct gctataagaa
     2101 ataccatatt aggacgtata gttagtccta ggtgtgaata tcaagcagga cataacaagg
     2161 taggatctct acagtacttg gcactagcag cattaataaa accaaaacag ataaagccac
     2221 ctttgcctag tgttaggaaa ctgacagagg acagatggaa caagccccag aagaccaagg
     2281 gccacagagg gagccataca atgaatggac actagagctt ttagaggaac ttaagagtga
     2341 agctgttaga cattttccta ggatatggct ccataactta ggacaacata tctatgaaac
     2401 ttacggggat acttgggcag gagtggaagc cataataaga attctgcaac aactgctgtt
     2461 tatccatttc agaattgggt gtcgacatag cagaataggc gttactcgac agaggagagc
     2521 aagaaatgga gccagtagat cctagactag agccctggaa gcatccagga agtcagccta
     2581 aaactgcttg taccaattgc tattgtaaaa agtgttgctt tcattgccaa gtttgtttca
     2641 tgacaaaagc cttaggcatc tcctatggca ggaagaagcg gagacagcga cgaagagctc
     2701 atcagaacag tcagactcat caagcttctc tatcaaagca gtaagtagta catgtaatgc
     2761 aacctataat agtagcaata gtagcattag tagtagcaat aataatagca atagttgtgt
     2821 ggtccatagt aatcatagaa tataggaaaa tattaagaca aagaaaaata gacaggttaa
     2881 ttgatagact aatagaaaga gcagaagaca gtggcaatga gagtgaagga gaagtatcag
     2941 cacttgtgga gatgggggtg gaaatggggc accatgctcc ttgggatatt gatgatctgt
     3001 agtgctacag aaaaattgtg ggtcaccgtc tattatgggg tacctgtgtg gaaggaagca
     3061 accaccactc tattttgtgc atcagatgct aaagcatata tgatacagag gtacataatg
     3121 tttgggccac acatgcctgt gtacccacag accccaaccc acaagaagta gtattggtaa
     3181 atgtgacaga aaattttaac atgtggaaaa atgacatggt agaacagatg catgaggata
     3241 taatcagttt atgggatcaa agcctaaagc catgtgtaaa attaacccca ctctgtgtta
     3301 gtttaaagtg cactgatttg aagaatgata ctaataccaa tagtagtagc gggagaatga
     3361 taatggagaa aggagagata aaaaactgct ctttcaatat cagcacaagc ataagagata
     3421 aggtgcagaa agaatatgca ttcttttata aacttgatat agtaccaata gataatacca
     3481 gctataggtt gataagttgt aacacctcag tcattacaca ggcctgtcca aaggtatcct
     3541 ttgagccaat tcccatacat tattgtgccc cggctggttt tgcgattcta aaatgtaata
     3601 ataagacgtt caatggaaca ggaccatgta caaatgtcag cacagtacaa tgtacacatg
     3661 gaatcaggcc agtagtatca actcaactgc tgttaaatgg cagtctagca gaagaagatg
     3721 tagtaattag atctgccaat ttcacagaca atgctaaaac cataatagta cagctgaaca
     3781 catctgtaga aattaattgt acaagaccca acaacaatac aagaaaaagt atccgtatcc
     3841 agaggggacc agggagagca tttgttacaa taggaaaaat aggaaatatg agacaagcac
     3901 attgtaacat tagtagagca aaatggaatg ccactttaaa acagatagct agcaaattaa
     3961 gagaacaatt tggaaataat aaaacaataa tctttaagca atcctcagga ggggacccag
     4021 aaattgtaac gcacagtttt aattgtggag gggaattttt ctactgtaat tcaacacaac
     4081 tgtttaatag tacttggttt aatagtactt ggagtactga agggtcaaat aacactgaag
     4141 gaagtgacac aatcacactc ccatgcagaa taaaacaatt tataaacatg tggcaggaag
     4201 taggaaaagc aatgtatgcc cctcccatca gtggacaaat tagatgttca tcaaatatta
     4261 ctgggctgct attaacaaga gatggtggta ataacaacaa tgggtccgag atcttcagac
     4321 ctggaggagg cgatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     4381 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     4441 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     4501 tgggcgcacg gtcaatgacg ctgacggtac aggccagaca attattgtct gatatagtgc
     4561 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     4621 tctggggcat caaacagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     4681 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     4741 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaataacatg acctggatgg
     4801 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     4861 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     4921 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     4981 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     5041 agggatattc accattatcg tttcagaccc acctcccaat cccgagggga cccgacaggc
     5101 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     5161 acggatcctt agcacttatc tgggacgatc tgcggagcct gtgcctcttc agctaccacc
     5221 gcttgagaga cttactcttg attgtaacga ggattgtgga acttctggga cgcagggggt
     5281 gggaagccct caaatattgg tggaatctcc tacagtattg gagtcaggaa ctaaagaata
     5341 gtgctgttag cttgctcaat gccacagcca tagcagtagc tgaggggaca gatagggtta
     5401 tagaagtatt acaagcagct tatagagcta ttcgccacat acctagaaga ataagacagg
     5461 gcttggaaag gattttgcta taagatgggt ggcgcggccg ctctggtgag caagggcgag
     5521 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     5581 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     5641 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     5701 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     5761 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     5821 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     5881 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     5941 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     6001 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     6061 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     6121 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     6181 gccgccggga tcactctcgg catggacgag ctgtacaagt aactcgagcc tagaaaaaca
     6241 tggagcaatc acaagtagca atacagcagc taacaatgct gcttgtgcct ggctagaagc
     6301 acaagaggag gaagaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     6361 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6421 aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     6481 cttccctgat tggcagaact acacaccagg gccaggggtc agatatccac tgacctttgg
     6541 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga
     6601 gaacaccagc ttgttacacc ctgtgagcct gcatggaatg gatgaccctg agagagaagt
     6661 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc
     6721 ggagtacttc aagaactgct gacatcgagc ttgctacaag ggactttccg ctggggactt
     6781 tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat gctgcatata
     6841 agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     6901 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6961 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7021 agtcagtgtg gaaaatctct agcaggccgg cccaattgac atgtgagcaa aaggccagca
     7081 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7141 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7201 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7261 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7321 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7381 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7441 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7501 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7561 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7621 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7681 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7741 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7801 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     7861 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7921 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7981 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     8041 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8101 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     8161 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8221 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8281 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8341 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8401 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8461 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8521 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8581 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     8641 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     8701 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     8761 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8821 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgc 
//
