LOCUS       pBS_Gabuzda_BstE       12628 bp ds-DNA     circular     30-JAN-2016
DEFINITION  pBluescript KS (-).
ACCESSION   urn.local...86-3xx88uo (modified)
VERSION     urn.local...86-3xx88uo
KEYWORDS    .
SOURCE      other sequences; artificial sequences; vectors
  ORGANISM  other sequences; artificial sequences; vectors. . . .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="pBluescript KS (-)"
                     /mol_type="other DNA"
                     /label=source:pBluescript KS (-)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1..448
                     /created_by="User"
                     /modified_by="User"
                     /label=pBS-KS
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      24..330
                     /label=f1_origin
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    443..448
                     /created_by="User"
                     /label=SspI
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    449..456
                     /created_by="User"
                     /label=PacI
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    457..1092
                     /Color="#77ffff"
                     /modified_by="User"
                     /label=5' long terminal repeat
                     /ApEinfo_fwdcolor=#ff5d6e
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    457..910
                     /created_by="User"
                     /label=5' U3
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(612..635)
                     /created_by="primer3"
                     /annotation_group="550266810326748e3caf66a-7c82-489b-b0e0-
                     6cdd7c1696a4"
                     /%GC="54.2"
                     /Tm="62.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GGCCTCTTCTACTTGCTCTGGTTC"
                     /Transferred_From="5'LTR_seq-R"
                     /Transferred_Similarity="100.00%"
                     /label=5'LTR_seq-R
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    781..790
                     /created_by="User"
                     /label=RBEIII
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    805..815
                     /created_by="User"
                     /label=NK-kBii
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    820..829
                     /created_by="User"
                     /label=NF-kBi
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    833..842
                     /created_by="User"
                     /label=Ssp1iii
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    844..853
                     /created_by="User"
                     /label=Ssp1ii
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    855..864
                     /created_by="User"
                     /label=Ssp1i
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    883..887
                     /created_by="User"
                     /label=TATA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    911..1007
                     /modified_by="User"
                     /label=5' R
                     /ApEinfo_fwdcolor=#a2ff71
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    911..968
                     /created_by="User"
                     /label=TAR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1008..1092
                     /created_by="User"
                     /label=5' U5
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1033..1040
                     /created_by="User"
                     /label=PAS Primer Activation Signal
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1093..1107
                     /Color="#ffddff"
                     /created_by="User"
                     /label=PBS
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1142..1279
                     /Color="#ffff55"
                     /created_by="User"
                     /label=Possible Psi Sequence
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1167..1172
                     /created_by="User"
                     /modified_by="User"
                     /label=DIS Dimerization Initiation Signal
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1171..1187
                     /Sequence="GCACGGCAAGAGGCGAG"
                     /Hairpin_Tm="64.0"
                     /Tm="61.2"
                     /Self_Dimer_Tm="18.2"
                     /%GC="70.6"
                     /created_by="primer3"
                     /label=NL4-3/8.9 Pre-Gag For
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1200..1201
                     /created_by="User"
                     /Color="#cc00cc"
                     /modified_by="User"
                     /label=5' Splice Donor
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            1246..2748
                     /modified_by="User"
                     /label=Gag
                     /ApEinfo_fwdcolor=#ffff80
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1246..1641
                     /created_by="User"
                     /label=MA p17
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1287..1288
                     /created_by="User"
                     /label=CG
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1642..2334
                     /created_by="User"
                     /label=CA p24
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1903..1921
                     /Sequence="GCAGGGCCTATTGCACCAG"
                     /Hairpin_Tm="48.3"
                     /Tm="61.1"
                     /Self_Dimer_Tm="18.3"
                     /%GC="63.2"
                     /created_by="primer3"
                     /label=NL4-3 Gag For 2
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1997..2021)
                     /Sequence="CCTACTGGGATAGGTGGATTATGTG"
                     /Hairpin_Tm="37.2"
                     /Tm="60.0"
                     /Self_Dimer_Tm="None"
                     /%GC="48.0"
                     /created_by="primer3"
                     /modified_by="User"
                     /note="Geneious type: primer_bind_reverse"
                     /label=Gag (CA) 751-776 Rev
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     2078..2097
                     /Sequence="GCCCTACCAGCATTCTGGAC"
                     /Hairpin_Tm="58.9"
                     /Tm="60.5"
                     /Self_Dimer_Tm="17.3"
                     /%GC="60.0"
                     /created_by="primer3"
                     /label=NL4-3 Gag 833-852
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2335..2376
                     /created_by="User"
                     /label=p2
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2377..2541
                     /created_by="User"
                     /modified_by="User"
                     /label=NC p7
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            2541..5552
                     /modified_by="User"
                     /label=Pol
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2542..2589
                     /created_by="User"
                     /label=p1
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2590..2745
                     /created_by="User"
                     /label=p6 Vpr binding
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     2600..2616
                     /Sequence="GACCAGAGCCAACAGCC"
                     /Hairpin_Tm="45.5"
                     /Tm="57.5"
                     /Self_Dimer_Tm="None"
                     /%GC="64.7"
                     /created_by="primer3"
                     /label=NL4-3 Prot For
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2709..3005
                     /created_by="User"
                     /label=PR p15
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2781..2783
                     /created_by="User"
                     /label=D25A PR mutant
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3006..4325
                     /created_by="User"
                     /label=RT p51
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3330..3346)
                     /Sequence="GCATCGCCCACATCCAG"
                     /Hairpin_Tm="None"
                     /Tm="58.1"
                     /Self_Dimer_Tm="None"
                     /%GC="64.7"
                     /created_by="primer3"
                     /note="Geneious type: primer_bind_reverse"
                     /label=NL4-3 Prot Rev
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     3330..3346
                     /Sequence="CTGGATGTGGGCGATGC"
                     /Hairpin_Tm="None"
                     /Tm="58.1"
                     /Self_Dimer_Tm="None"
                     /%GC="64.7"
                     /created_by="primer3"
                     /label=NL4-3 Pol 789-806 For
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3558..3563
                     /created_by="User"
                     /modified_by="User"
                     /label=D185K D186L RT mutant
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     3993..4009
                     /Sequence="CAGAAGCAGGGGCAAGG"
                     /Hairpin_Tm="44.6"
                     /Tm="57.5"
                     /Self_Dimer_Tm="None"
                     /%GC="64.7"
                     /created_by="primer3"
                     /modified_by="User"
                     /label=NL4-3 Pol 1452-1469
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4326..4685
                     /created_by="User"
                     /label=RNase H p15
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4686..5549
                     /created_by="User"
                     /label=IN p31
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     4947..4968
                     /Sequence="GTAATTCCAGCAGAGACAGGGC"
                     /Hairpin_Tm="None"
                     /Tm="61.0"
                     /Self_Dimer_Tm="None"
                     /%GC="54.5"
                     /created_by="primer3"
                     /modified_by="User"
                     /label=NL4-3 Pol 2406-2428 For
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5031..5033
                     /created_by="User"
                     /label=D116A integrase mutant
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5237..5254
                     /created_by="User"
                     /label=Tentative cPPT
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(5367..5368)
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label=3' Splice Acceptor
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5497..6075
                     /label=Vif
                     /ApEinfo_fwdcolor=#f71cff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     5651..5675
                     /Sequence="GTTCAGAAGTACACATCCCACTAGG"
                     /Hairpin_Tm="None"
                     /Tm="60.6"
                     /Self_Dimer_Tm="None"
                     /%GC="48.0"
                     /created_by="primer3"
                     /label=NL4-3 Vif 5195 For
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(5844..5845)
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label=3' Splice Acceptor(1)
                     /ApEinfo_label=3' Splice Acceptor
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5920..5921
                     /created_by="User"
                     /Color="#cc00cc"
                     /modified_by="User"
                     /label=5' Splice Donor(1)
                     /ApEinfo_label=5' Splice Donor
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     5976..5997
                     /Sequence="GCCACCTTTGCCTAGTGTTAGG"
                     /Hairpin_Tm="47.8"
                     /Tm="61.2"
                     /Self_Dimer_Tm="None"
                     /%GC="54.5"
                     /modified_by="User"
                     /label=NL4-3 Vif 5520 For
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6015..6198
                     /label=Vpr
                     /ApEinfo_fwdcolor=#cbcccf
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8827..8872
                     /label=Tat
                     /ApEinfo_fwdcolor=#fff51f
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    join(8827..8923,8928..9104)
                     /label=Rev
                     /ApEinfo_fwdcolor=#ec73ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8924..8927
                     /label=Rev(1)
                     /ApEinfo_label=Rev
                     /ApEinfo_fwdcolor=#ec73ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            join(6788..8923,8928..9247)
                     /modified_by="User"
                     /label=Env Polyprotein
                     /ApEinfo_fwdcolor=#ffa729
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            8924..8927
                     /modified_by="User"
                     /label=Env Polyprotein(1)
                     /ApEinfo_label=Env Polyprotein
                     /ApEinfo_fwdcolor=#ffa729
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6788..8205
                     /created_by="User"
                     /label=gp120 Surface
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6856..6857
                     /note="Geneious type: Editing History Insertion"
                     /label=Geneious type: Editing History Insertion
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7069..7146
                     /created_by="User"
                     /modified_by="User"
                     /label=V1 loop
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7150..7260
                     /created_by="User"
                     /modified_by="User"
                     /label=V2 loop
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     7312..7332
                     /Sequence="CCCATACATTATTGTGCCCCG"
                     /Hairpin_Tm="36.5"
                     /Tm="59.1"
                     /Self_Dimer_Tm="None"
                     /%GC="52.4"
                     /created_by="primer3"
                     /label=NL4-3 Env For (2)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     7428..7452
                     /created_by="User"
                     /Sequence="GCCAGTAGTATCAACTCAACTGCTG"
                     /label=Env 759-783 For
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7558..7665
                     /created_by="User"
                     /modified_by="User"
                     /label=V3 loop
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7825..7926
                     /created_by="User"
                     /label=V4 Loop
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8050..8082
                     /created_by="User"
                     /label=V5 Loop
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     8078..8097
                     /Sequence="GACCTGGAGGAGGCGATATG"
                     /Hairpin_Tm="47.6"
                     /Tm="59.4"
                     /Self_Dimer_Tm="None"
                     /%GC="60.0"
                     /created_by="primer3"
                     /label=NL4-3 Env For (3)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     8146..8168
                     /created_by="User"
                     /Sequence="GAACCATTAGGAGTAGCACCCAC"
                     /label=Env 1480-1502 For
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8158..8509
                     /created_by="User"
                     /modified_by="User"
                     /label=RRE
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    join(8206..8923,8928..9244)
                     /created_by="User"
                     /label=gp41 Transmembrane
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8924..8927
                     /created_by="User"
                     /label=gp41 Transmembrane(1)
                     /ApEinfo_label=gp41 Transmembrane
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8647..8721
                     /created_by="User"
                     /label=MPER sequence
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8725..8784
                     /created_by="User"
                     /modified_by="User"
                     /label=TM domain
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(8825..8826)
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label=3' Splice Acceptor(2)
                     /ApEinfo_label=3' Splice Acceptor
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(8884..8907)
                     /created_by="User"
                     /Sequence="GGATCTGTCTCTGTCTCTCTCTCC"
                     /Hairpin_Tm="None"
                     /Tm="61.0"
                     /Self_Dimer_Tm="None"
                     /%GC="54.2"
                     /created_by="primer3"
                     /modified_by="User"
                     /note="Geneious type: primer_bind_reverse"
                     /label=Env 2218-2241 Rev
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     8884..8907
                     /created_by="User"
                     /Sequence="GGAGAGAGAGACAGAGACAGATCC"
                     /label=Env 2218-2241 For
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(8975..8976)
                     /created_by="User"
                     /Color="#ff9911"
                     /modified_by="User"
                     /label=3' Splice Acceptor maybe
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     localization    8977..9006
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="HIV_Rev_NES loc"
                     /Transferred_Similarity="100.00%"
                     /label=HIV_Rev_NES loc
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     9143..9160
                     /Sequence="GCTGAGGGGACAGATAGG"
                     /Hairpin_Tm="39.9"
                     /Tm="55.7"
                     /Self_Dimer_Tm="None"
                     /%GC="61.1"
                     /created_by="primer3"
                     /label=NL4-3 Nef For
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    9258..9265
                     /label=Not1
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#80ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    9267..9986
                     /label=GFP
                     /ApEinfo_fwdcolor=#00ff40
                     /ApEinfo_revcolor=#80ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            9993..10506
                     /created_by="User"
                     /label=Nef
                     /ApEinfo_fwdcolor=#dc5aff
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     LTR             10175..10808
                     /modified_by="User"
                     /label=3' long terminal repeat
                     /ApEinfo_fwdcolor=#bdbdbd
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    10175..10628
                     /created_by="User"
                     /label=5' U3(1)
                     /ApEinfo_label=5' U3
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     10404..10425
                     /created_by="primer3"
                     /annotation_group="550270890500630f909325d-e334-42e9-8008-
                     82767c572c9a"
                     /%GC="54.5"
                     /Tm="59.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GGATGACCCTGAGAGAGAAGTG"
                     /Transferred_From="Nef-3'LTR_seq-F"
                     /Transferred_Similarity="100.00%"
                     /label=Nef-3'LTR_seq-F
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    10499..10508
                     /created_by="User"
                     /label=RBEIII(1)
                     /ApEinfo_label=RBEIII
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    10523..10533
                     /created_by="User"
                     /label=NF-kBii
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    10538..10547
                     /created_by="User"
                     /label=NF-kBi(1)
                     /ApEinfo_label=NF-kBi
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    10551..10560
                     /created_by="User"
                     /label=Sp1iii
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    10562..10571
                     /created_by="User"
                     /label=Ssp1ii(1)
                     /ApEinfo_label=Ssp1ii
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    10573..10582
                     /created_by="User"
                     /label=Ssp1i(1)
                     /ApEinfo_label=Ssp1i
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    10629..10725
                     /label=3' copy
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    10629..10686
                     /created_by="User"
                     /label=TAR(1)
                     /ApEinfo_label=TAR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(10696..10725)
                     /created_by="primer3"
                     /annotation_group="550270223354725f11562b5-34c6-44f8-ad2a-
                     9144881716fd"
                     /%GC="46.7"
                     /Tm="67.7"
                     /Hairpin_Tm="33.3"
                     /Self_Dimer_Tm="28.2"
                     /Sequence="TGAAGCACTCAAGGCAAGCTTTATTGAGGC"
                     /Transferred_From="JFSG_inner_Env_antisense"
                     /Transferred_Similarity="100.00%"
                     /label=JFSG_inner_Env_antisense
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(10700..10731)
                     /created_by="primer3"
                     /annotation_group="550270280181557095ea68b-18b7-4f3c-b24f-
                     8d6318bcb094"
                     /%GC="40.6"
                     /Tm="65.9"
                     /Hairpin_Tm="57.3"
                     /Self_Dimer_Tm="20.8"
                     /Sequence="ACTACTTGAAGCACTCAAGGCAAGCTTTATTG"
                     /Transferred_From="JFSG_outer_Env_antisense"
                     /Transferred_Similarity="100.00%"
                     /label=JFSG_outer_Env_antisense
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    10701..10706
                     /label=PolyA signal
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    10726..10810
                     /created_by="User"
                     /label=5' U5(1)
                     /ApEinfo_label=5' U5
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    10751..10758
                     /created_by="User"
                     /label=PAS Primer Activation Signal(1)
                     /ApEinfo_label=PAS Primer Activation Signal
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    10809..10816
                     /created_by="User"
                     /label=FseI
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     source          10823..12628
                     /organism="pBluescript KS (-)"
                     /mol_type="other DNA"
                     /label=source:pBluescript KS (-)(1)
                     /ApEinfo_label=source:pBluescript KS (-)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    10823..12628
                     /created_by="User"
                     /modified_by="User"
                     /label=pBS-KS(1)
                     /ApEinfo_label=pBS-KS
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    10823..10828
                     /created_by="User"
                     /label=AflIII
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(10866..11548)
                     /Transferred_From="ColE1 origin , ColE1 origin  rev"
                     /Transferred_Similarity="99.71%"
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(11643..12503)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label=ORF frame 3
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            complement(11643..12503)
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(12545..12573)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label=AmpR_promoter
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6199..6787
                     /label=Rev kill
                     /ApEinfo_fwdcolor=#00ff40
                     /ApEinfo_revcolor=#80ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      421 aatttaacgc gaattttaac aaaatatttt aattaatgga agggctaatt tggtcccaaa
      481 aaagacaaga gatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
      541 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgcttcaagt
      601 tagtaccagt tgaaccagag caagtagaag aggccaaata aggagagaag aacagcttgt
      661 tacaccctat gagccagcat gggatggagg acccggaggg agaagtatta gtgtggaagt
      721 ttgacagcct cctagcattt cgtcacatgg cccgagagct gcatccggag tactacaaag
      781 actgctgaca tcgagctttc tacaagggac tttccgctgg ggactttcca gggaggtgtg
      841 gcctgggcgg gactggggag tggcgagccc tcagatgcta catataagca gctgcttttt
      901 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
      961 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgctcaaagt agtgtgtgcc
     1021 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     1081 atctctagca gtggcgcccg aacagggact tgaaagcgaa agtaaagcca gaggagatct
     1141 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     1201 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     1261 tcggtattaa gcgggggaga attagataaa tgggaaaaaa ttcggttaag gccaggggga
     1321 aagaaacaat ataaactaaa acatatagta tgggcaagca gggagctaga acgattcgca
     1381 gttaatcctg gccttttaga gacatcagaa ggctgtagac aaatactggg acagctacaa
     1441 ccatcccttc agacaggatc agaagaactt agatcattat ataatacaat agcagtcctc
     1501 tattgtgtgc atcaaaggat agatgtaaaa gacaccaagg aagccttaga taagatagag
     1561 gaagagcaaa acaaaagtaa gaaaaaggca cagcaagcag cagctgacac aggaaacaac
     1621 agccaggtca gccaaaatta ccctatagtg cagaacctcc aggggcaaat ggtacatcag
     1681 gccatatcac ctagaacttt aaatgcatgg gtaaaagtag tagaagagaa ggctttcagc
     1741 ccagaagtaa tacccatgtt ttcagcatta tcagaaggag ccaccccaca agatttaaat
     1801 accatgctaa acacagtggg gggacatcaa gcagccatgc aaatgttaaa agagaccatc
     1861 aatgaggaag ctgcagaatg ggatagattg catccagtgc atgcagggcc tattgcacca
     1921 ggccagatga gagaaccaag gggaagtgac atagcaggaa ctactagtac ccttcaggaa
     1981 caaataggat ggatgacaca taatccacct atcccagtag gagaaatcta taaaagatgg
     2041 ataatcctgg gattaaataa aatagtaaga atgtatagcc ctaccagcat tctggacata
     2101 agacaaggac caaaggaacc ctttagagac tatgtagacc gattctataa aactctaaga
     2161 gccgagcaag cttcacaaga ggtaaaaaat tggatgacag aaaccttgtt ggtccaaaat
     2221 gcgaacccag attgtaagac tattttaaaa gcattgggac caggagcgac actagaagaa
     2281 atgatgacag catgtcaggg agtgggggga cccggccata aagcaagagt tttggctgaa
     2341 gcaatgagcc aagtaacaaa tccagctacc ataatgatac agaaaggcaa ttttaggaac
     2401 caaagaaaga ctgttaagtg tttcaattgt ggcaaagaag ggcacatagc caaaaattgc
     2461 agggccccta ggaaaaaggg ctgttggaaa tgtggaaagg aaggacacca aatgaaagat
     2521 tgtactgaga gacaggctaa ttttttaggg aagatctggc cttcccacaa gggaaggcca
     2581 gggaattttc ttcagagcag accagagcca acagccccac cagaagagag cttcaggttt
     2641 ggggaagaga caacaactcc ctctcagaag caggagccga tagacaagga actgtatcct
     2701 ttagcttccc tcagatcact ctttggcagc gacccctcgt cacaataaag ataggggggc
     2761 aattaaagga agctctatta gatacaggag cagatgatac agtattagaa gaaatgaatt
     2821 tgccaggaag atggaaacca aaaatgatag ggggaattgg aggttttatc aaagtaggac
     2881 agtatgatca gatactcata gaaatctgcg gacataaagc tataggtaca gtattagtag
     2941 gacctacacc tgtcaacata attggaagaa atctgttgac tcagattggc tgcactttaa
     3001 attttcccat tagtcctatt gagactgtac cagtaaaatt aaagccagga atggatggcc
     3061 caaaagttaa acaatggcca ttgacagaag aaaaaataaa agcattagta gaaatttgta
     3121 cagaaatgga aaaggaagga aaaatttcaa aaattgggcc tgaaaatcca tacaatactc
     3181 cagtatttgc cataaagaaa aaagacagta ctaaatggag aaaattagta gatttcagag
     3241 aacttaataa gagaactcaa gatttctggg aagttcaatt aggaatacca catcctgcag
     3301 ggttaaaaca gaaaaaatca gtaacagtac tggatgtggg cgatgcatat ttttcagttc
     3361 ccttagataa agacttcagg aagtatactg catttaccat acctagtata aacaatgaga
     3421 caccagggat tagatatcag tacaatgtgc ttccacaggg atggaaagga tcaccagcaa
     3481 tattccagtg tagcatgaca aaaatcttag agccttttag aaaacaaaat ccagacatag
     3541 tcatctatca atacatggat gatttgtatg taggatctga cttagaaata gggcagcata
     3601 gaacaaaaat agaggaactg agacaacatc tgttgaggtg gggatttacc acaccagaca
     3661 aaaaacatca gaaagaacct ccattccttt ggatgggtta tgaactccat cctgataaat
     3721 ggacagtaca gcctatagtg ctgccagaaa aggacagctg gactgtcaat gacatacaga
     3781 aattagtggg aaaattgaat tgggcaagtc agatttatgc agggattaaa gtaaggcaat
     3841 tatgtaaact tcttagggga accaaagcac taacagaagt agtaccacta acagaagaag
     3901 cagagctaga actggcagaa aacagggaga ttctaaaaga accggtacat ggagtgtatt
     3961 atgacccatc aaaagactta atagcagaaa tacagaagca ggggcaaggc caatggacat
     4021 atcaaattta tcaagagcca tttaaaaatc tgaaaacagg aaaatatgca agaatgaagg
     4081 gtgcccacac taatgatgtg aaacaattaa cagaggcagt acaaaaaata gccacagaaa
     4141 gcatagtaat atggggaaag actcctaaat ttaaattacc catacaaaag gaaacatggg
     4201 aagcatggtg gacagagtat tggcaagcca cctggattcc tgagtgggag tttgtcaata
     4261 cccctccctt agtgaagtta tggtaccagt tagagaaaga acccataata ggagcagaaa
     4321 ctttctatgt agatggggca gccaataggg aaactaaatt aggaaaagca ggatatgtaa
     4381 ctgacagagg aagacaaaaa gttgtccccc taacggacac aacaaatcag aagactgagt
     4441 tacaagcaat tcatctagct ttgcaggatt cgggattaga agtaaacata gtgacagact
     4501 cacaatatgc attgggaatc attcaagcac aaccagataa gagtgaatca gagttagtca
     4561 gtcaaataat agagcagtta ataaaaaagg aaaaagtcta cctggcatgg gtaccagcac
     4621 acaaaggaat tggaggaaat gaacaagtag atgggttggt cagtgctgga atcaggaaag
     4681 tactattttt agatggaata gataaggccc aagaagaaca tgagaaatat cacagtaatt
     4741 ggagagcaat ggctagtgat tttaacctac cacctgtagt agcaaaagaa atagtagcca
     4801 gctgtgataa atgtcagcta aaaggggaag ccatgcatgg acaagtagac tgtagcccag
     4861 gaatatggca gctagattgt acacatttag aaggaaaagt tatcttggta gcagttcatg
     4921 tagccagtgg atatatagaa gcagaagtaa ttccagcaga gacagggcaa gaaacagcat
     4981 acttcctctt aaaattagca ggaagatggc cagtaaaaac agtacataca gacaatggca
     5041 gcaatttcac cagtactaca gttaaggccg cctgttggtg ggcggggatc aagcaggaat
     5101 ttggcattcc ctacaatccc caaagtcaag gagtaataga atctatgaat aaagaattaa
     5161 agaaaattat aggacaggta agagatcagg ctgaacatct taagacagca gtacaaatgg
     5221 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     5281 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     5341 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg aaaggaccag
     5401 caaagctcct ctggaaaggt gaaggggcag tagtaataca agataatagt gacataaaag
     5461 tagtgccaag aagaaaagca aagatcatca gggattatgg aaaacagatg gcaggtgatg
     5521 attgtgtggc aagtagacag gatgaggatt aacacatgga aaagattagt aaaacaccat
     5581 atgtatattt caaggaaagc taaggactgg ttttatagac atcactatga aagtactaat
     5641 ccaaaaataa gttcagaagt acacatccca ctaggggatg ctaaattagt aataacaaca
     5701 tattggggtc tgcatacagg agaaagagac tggcatttgg gtcagggagt ctccatagaa
     5761 tggaggaaaa agagatatag cacacaagta gaccctgacc tagcagacca actaattcat
     5821 ctgcactatt ttgattgttt ttcagaatct gctataagaa ataccatatt aggacgtata
     5881 gttagtccta ggtgtgaata tcaagcagga cataacaagg taggatctct acagtacttg
     5941 gcactagcag cattaataaa accaaaacag ataaagccac ctttgcctag tgttaggaaa
     6001 ctgacagagg acagatggaa caagccccag aagaccaagg gccacagagg gagccataca
     6061 atgaatggac actagagctt ttagaggaac ttaagagtga agctgttaga cattttccta
     6121 ggatatggct ccataactta ggacaacata tctatgaaac ttacggggat acttgggcag
     6181 gagtggaagc cataataaGA ATTCTGCAAC AACTGCTGTT TATCCATTTC AGAATTGGGT
     6241 GTCGACATAG CAGAATAGGC GTTACTCGAC AGAGGAGAGC AAGAAATGGA GCCAGTAGAT
     6301 CCTAGACTAG AGCCCTGGAA GCATCCAGGA AGTCAGCCTA AAACTGCTTG TACCAATTGC
     6361 TATTGTAAAA AGTGTTGCTT TCATTGCCAA GTTTGTTTCA TGACAAAAGC CTTAGGCATC
     6421 TCCTACGGCA GGAAGAAGCG GAGACAGCGA CGAAGAGCTC ATCAGAACAG TCAGACTCAT
     6481 CAGGCTTCTC TAAGCAAGCA GTAAGTAGTA CATGTAATGC AACCTATAAT AGTAGCAATA
     6541 GTAGCATTAG TAGTAGCAAT AATAATAGCA ATAGTTGTGT GGTCCATAGT AATCATAGAA
     6601 TATAGGAAAA TATTAAGACA AAGAAAAATA GACAGGTTAA TTGATAGACT AATAGAAAGA
     6661 GCAGAAGACA GTGGCAATGA GAGTGAAGGA GAAGTATCAG CACTTGTGGA GATGGGGGTG
     6721 GAAATGGGGC ACCATGCTCC TTGGGATATT GATGATCTGT AGTGCTACAG AAAAATTGTG
     6781 GGTCACCgtc tattatgggg tacctgtgtg gaaggaagca accaccactc tattttgtgc
     6841 atcagatgct aaagcatata tgatacagag gtacataatg tttgggccac acatgcctgt
     6901 gtacccacag accccaaccc acaagaagta gtattggtaa atgtgacaga aaattttaac
     6961 atgtggaaaa atgacatggt agaacagatg catgaggata taatcagttt atgggatcaa
     7021 agcctaaagc catgtgtaaa attaacccca ctctgtgtta gtttaaagtg cactgatttg
     7081 aagaatgata ctaataccaa tagtagtagc gggagaatga taatggagaa aggagagata
     7141 aaaaactgct ctttcaatat cagcacaagc ataagagata aggtgcagaa agaatatgca
     7201 ttcttttata aacttgatat agtaccaata gataatacca gctataggtt gataagttgt
     7261 aacacctcag tcattacaca ggcctgtcca aaggtatcct ttgagccaat tcccatacat
     7321 tattgtgccc cggctggttt tgcgattcta aaatgtaata ataagacgtt caatggaaca
     7381 ggaccatgta caaatgtcag cacagtacaa tgtacacatg gaatcaggcc agtagtatca
     7441 actcaactgc tgttaaatgg cagtctagca gaagaagatg tagtaattag atctgccaat
     7501 ttcacagaca atgctaaaac cataatagta cagctgaaca catctgtaga aattaattgt
     7561 acaagaccca acaacaatac aagaaaaagt atccgtatcc agaggggacc agggagagca
     7621 tttgttacaa taggaaaaat aggaaatatg agacaagcac attgtaacat tagtagagca
     7681 aaatggaatg ccactttaaa acagatagct agcaaattaa gagaacaatt tggaaataat
     7741 aaaacaataa tctttaagca atcctcagga ggggacccag aaattgtaac gcacagtttt
     7801 aattgtggag gggaattttt ctactgtaat tcaacacaac tgtttaatag tacttggttt
     7861 aatagtactt ggagtactga agggtcaaat aacactgaag gaagtgacac aatcacactc
     7921 ccatgcagaa taaaacaatt tataaacatg tggcaggaag taggaaaagc aatgtatgcc
     7981 cctcccatca gtggacaaat tagatgttca tcaaatatta ctgggctgct attaacaaga
     8041 gatggtggta ataacaacaa tgggtccgag atcttcagac ctggaggagg cgatatgagg
     8101 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     8161 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     8221 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcacg gtcaatgacg
     8281 ctgacggtac aggccagaca attattgtct gatatagtgc agcagcagaa caatttgctg
     8341 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caaacagctc
     8401 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     8461 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     8521 aaatctctgg aacagatttg gaataacatg acctggatgg agtgggacag agaaattaac
     8581 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     8641 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     8701 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     8761 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     8821 tttcagaccc acctcccaat cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     8881 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcgat ccttagcact
     8941 tatctgggac gatctgcgga gcctgtgcct cttcagctac caccgcttga gagacttact
     9001 cttgattgta acgaggattg tggaacttct gggacgcagg gggtgggaag ccctcaaata
     9061 ttggtggaat ctcctacagt attggagtca ggaactaaag aatagtgctg ttaacttgct
     9121 caatgccaca gccatagcag tagctgaggg gacagatagg gttatagaag tattacaagc
     9181 agcttataga gctattcgcc acatacctag aagaataaga cagggcttgg aaaggatttt
     9241 gctataagat gggtggcgcg gccgctctgg tgagcaaggg cgaggagctg ttcaccgggg
     9301 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     9361 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     9421 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     9481 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     9541 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     9601 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     9661 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     9721 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     9781 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     9841 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     9901 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     9961 tcggcatgga cgagctgtac aagtaactcg agcctagaaa aacatggagc aatcacaagt
    10021 agcaatacag cagctaacaa tgctgcttgt gcctggctag aagcacaaga ggaggaagag
    10081 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
    10141 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaaaga
    10201 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag
    10261 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
    10321 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta
    10381 caccctgtga gcctgcatgg aatggatgac cctgagagag aagtgttaga gtggaggttt
    10441 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac
    10501 tgctgacatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc
    10561 ctgggcggga ctggggagtg gcgagccctc agatgctgca tataagcagc tgctttttgc
    10621 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
    10681 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
    10741 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
    10801 ctctagcagg ccggcccaat tgacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    10861 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    10921 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    10981 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    11041 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    11101 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    11161 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    11221 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    11281 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
    11341 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    11401 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    11461 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    11521 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    11581 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    11641 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    11701 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    11761 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    11821 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    11881 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    11941 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    12001 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    12061 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    12121 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    12181 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    12241 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    12301 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    12361 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    12421 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    12481 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    12541 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    12601 gttccgcgca catttccccg aaaagtgc   
//
