LOCUS       Exported               14820 bp ds-DNA     circular SYN 07-SEP-2017
DEFINITION  pHR-SFFV-dCas9-BFP-KRAB.
ACCESSION   urn.local...12-3uy0ryr
VERSION     .
KEYWORDS    IGI-P0220-pHR-EF1a-dCas9-HA-BFP-KRAB-NLS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 7, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14820
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    454..634
                     /label=HIV-1_5_LTR
                     /note="HIV-1_5_LTR"
     misc_feature    454..634
                     /label=truncHIV-1_3_LTR
                     /note="truncHIV-1_3_LTR"
     misc_feature    745..789
                     /label=HIV-1_psi_pack
                     /note="HIV-1_psi_pack"
     misc_feature    1303..1535
                     /label=RRE
                     /note="RRE"
     misc_feature    2051..2200
                     /label=cPPT/CTS
                     /note="cPPT/CTS"
     misc_feature    2053..2068
                     /label=cPPT
                     /note="cPPT"
     misc_feature    2228..3398
                     /label=hEF1alpha promoter
                     /note="hEF1alpha promoter"
     CDS             join(3454..8580,8620..8622)
                     /codon_start=1
                     /label=dCas9-HA-NLS-BFP-KRAB
                     /note="dCas9-HA-NLS-BFP-KRAB"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGDAYPYDVPDYAEDPKKKRKVDGIGSGSNGSSGSSE
                     LIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVEGGPLPFAFDILATSF
                     LYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKI
                     RGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLVGGSHLIANIKTTYRS
                     KKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPSKLGHKLNGGGGGM
                     DAKSLTAWSRTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGYQLTKP
                     DVILRLEKGEEP"
     misc_feature    7561..7587
                     /label=HA
                     /note="HA"
     misc_feature    7654..8349
                     /label=BFP
                     /note="BFP"
     misc_feature    join(8350..8580,8620..8622)
                     /label=KRAB
                     /note="KRAB"
     CDS             8581..8619
                     /codon_start=1
                     /label=dCas9-HA-NLS-BFP-KRAB(1)
                     /note="dCas9-HA-NLS-BFP-KRAB(1)"
                     /translation="MLGSGSPKKKRKV"
     misc_feature    8599..8619
                     /label=SV40 NLS(1)
                     /note="SV40 NLS(1)"
     misc_feature    8605..8619
                     /label=NLS(1)
                     /note="NLS(1)"
     misc_feature    8646..8653
                     /label=Sbf1 site
                     /note="Sbf1 site"
     misc_feature    8685..9272
                     /label=WPRE
                     /note="WPRE"
     misc_feature    complement(9275..9291)
                     /label=pBluescriptKS_primer
                     /note="pBluescriptKS_primer"
     misc_feature    9364..9385
                     /label=U3PPT
                     /note="U3PPT"
     misc_feature    9364..9379
                     /label=cPPT(1)
                     /note="cPPT(1)"
     misc_feature    9381..9433
                     /label=delta_U3
                     /note="delta_U3"
     misc_feature    9434..9614
                     /label=HIV-1_5_LTR(1)
                     /note="HIV-1_5_LTR(1)"
     misc_feature    9434..9614
                     /label=truncHIV-1_3_LTR(1)
                     /note="truncHIV-1_3_LTR(1)"
     misc_feature    complement(9640..9657)
                     /label=Sp6_primer
                     /note="Sp6_primer"
     gene            9895..10158
                     /gene="tet (300 - 563)"
                     /label=tet (300 - 563)
                     /note="tet (300 - 563)"
     misc_feature    complement(9947..9966)
                     /label=pBRrevBam_primer
                     /note="pBRrevBam_primer"
     misc_feature    10299..10321
                     /label=pGEX_3_primer
                     /note="pGEX_3_primer"
     promoter        10480..10508
                     /label=AmpR_promoter
                     /note="AmpR_promoter"
     gene            10550..11410
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="Ampicillin"
     rep_origin      11565..12184
                     /label=pBR322_origin
                     /note="pBR322_origin"
     misc_feature    complement(12410..12430)
                     /label=pBABE_3_primer
                     /note="pBABE_3_primer"
     misc_feature    complement(12416..12869)
                     /label=SV40_enhancer
                     /note="SV40_enhancer"
     promoter        12428..12696
                     /label=SV40_promoter
                     /note="SV40_promoter"
     rep_origin      12595..12672
                     /label=SV40_origin
                     /note="SV40_origin"
     misc_feature    12657..12676
                     /label=SV40pro_F_primer
                     /note="SV40pro_F_primer"
     misc_feature    13877..13892
                     /label=SV40_int
                     /note="SV40_int"
     misc_feature    13898..13945
                     /label=SV40_3_splice
                     /note="SV40_3_splice"
     CDS             14022..14495
                     /codon_start=1
                     /label=ORF frame 1
                     /note="ORF frame 1"
                     /translation="MPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSN
                     RTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYA
                     QKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFTCFKKPPTPPPEPET"
     misc_feature    14073..14093
                     /label=SV40 NLS
                     /note="SV40 NLS"
     misc_feature    14079..14093
                     /label=NLS
                     /note="NLS"
     terminator      14521..14652
                     /label=SV40_PA_terminator
                     /note="SV40_PA_terminator"
     misc_feature    14609..14628
                     /label=EBV_rev_primer
                     /note="EBV_rev_primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtgaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc
     2041 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2101 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2161 aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc
     2221 gaattctgtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     2281 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     2341 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     2401 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
     2461 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
     2521 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2581 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2641 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2701 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2761 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2821 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     2881 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     2941 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3001 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3061 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3121 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3181 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3241 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3301 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3361 gtggttcaaa gtttttttct tccatttcag gtgtcgtgga attctcacgc gtctgcagga
     3421 tatcaagctt gcggtaccgc gggcccggcc accatggaca agaagtacag catcggcctg
     3481 gccatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc
     3541 aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcggc
     3601 gccctgctgt tcgacagcgg agaaacagcc gaggccaccc ggctgaagag aaccgccaga
     3661 agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag
     3721 atggccaagg tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag
     3781 gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac
     3841 gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc
     3901 gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg
     3961 atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat ccagctggtg
     4021 cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag
     4081 gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat cgcccagctg
     4141 cccggcgaga agaagaatgg cctgttcggc aacctgattg ccctgagcct gggcctgacc
     4201 cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct gagcaaggac
     4261 acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta cgccgacctg
     4321 tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct gagagtgaac
     4381 accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga cgagcaccac
     4441 caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa gtacaaagag
     4501 attttcttcg accagagcaa gaacggctac gccggctaca tcgatggcgg agccagccag
     4561 gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac cgaggaactg
     4621 ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga caacggcagc
     4681 atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca ggaagatttt
     4741 tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc
     4801 tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac cagaaagagc
     4861 gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc cagcgcccag
     4921 agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa ggtgctgccc
     4981 aagcacagcc tgctgtacga gtacttcacc gtgtacaacg agctgaccaa agtgaaatac
     5041 gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa agccatcgtg
     5101 gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga ggactacttc
     5161 aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg gttcaacgcc
     5221 tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt cctggacaat
     5281 gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt tgaggacaga
     5341 gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa agtgatgaag
     5401 cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct gatcaacggc
     5461 atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga cggcttcgcc
     5521 aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga ggacatccag
     5581 aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa tctggccggc
     5641 agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga gctcgtgaaa
     5701 gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga gaaccagacc
     5761 acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa
     5821 gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct gcagaacgag
     5881 aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca ggaactggac
     5941 atcaaccggc tgtccgacta cgatgtggac gctatcgtgc ctcagagctt tctgaaggac
     6001 gactccatcg ataacaaagt gctgactcgg agcgacaaga accggggcaa gagcgacaac
     6061 gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcgccagct gctgaatgcc
     6121 aagctgatta cccagaggaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc
     6181 gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag
     6241 cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa cgacaaactg
     6301 atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat
     6361 ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg
     6421 aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg
     6481 tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc
     6541 ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag
     6601 attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaaca
     6661 ggcgagatcg tgtgggataa gggccgggac tttgccaccg tgcggaaagt gctgtctatg
     6721 ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct
     6781 atcctgccca agaggaacag cgacaagctg atcgccagaa agaaggactg ggaccctaag
     6841 aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt ggccaaagtg
     6901 gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat caccatcatg
     6961 gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg ctacaaagaa
     7021 gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct ggaaaacggc
     7081 cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc
     7141 tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa gggctccccc
     7201 gaggataatg agcagaaaca gctgtttgtg gaacagcaca aacactacct ggacgagatc
     7261 atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa tctggacaag
     7321 gtgctgagcg cctacaacaa gcacagagac aagcctatca gagagcaggc cgagaatatc
     7381 atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta ctttgacacc
     7441 accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac cctgatccac
     7501 cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggcgacgcc
     7561 tatccctatg acgtgcccga ttatgccgaa gatcctaaga aaaagcggaa agtggacggc
     7621 attggtagtg ggagcaacgg cagcagcgga tccagcgagc tgattaagga gaacatgcac
     7681 atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac atccgagggc
     7741 gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct
     7801 ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa gaccttcatc
     7861 aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg cttcacatgg
     7921 gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc
     7981 caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac atccaacggc
     8041 cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct gtaccccgct
     8101 gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg gagccatctg
     8161 atcgcaaaca tcaagaccac atatagatcc aagaaacccg ctaagaacct caagatgcct
     8221 ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacaa cgagacctac
     8281 gtcgagcagc acgaggtggc agtggccaga tactgcgacc tccctagcaa actggggcac
     8341 aagcttaatg gcggtggcgg agggatggat gctaagtcac taactgcctg gtcccggaca
     8401 ctggtgacct tcaaggatgt atttgtggac ttcaccaggg aggagtggaa gctgctggac
     8461 actgctcagc agatcgtgta cagaaatgtg atgctggaga actataagaa cctggtttcc
     8521 ttgggttatc agcttactaa gccagatgtg atcctccggt tggagaaggg agaagagccc
     8581 agcctgggca gcggctcccc caagaaaaaa cgcaaggtgt agtaaggccg cgactctaga
     8641 gtcgacctgc aggcatgcaa gcttgatatc aagcttatcg ataatcaacc tctggattac
     8701 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     8761 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     8821 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     8881 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     8941 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     9001 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     9061 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     9121 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     9181 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     9241 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg agggaattaa
     9301 ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     9361 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     9421 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     9481 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     9541 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     9601 gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt
     9661 atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac
     9721 aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact
     9781 gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac
     9841 ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga
     9901 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
     9961 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
    10021 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
    10081 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
    10141 gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc
    10201 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
    10261 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
    10321 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
    10381 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
    10441 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
    10501 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
    10561 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
    10621 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
    10681 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
    10741 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
    10801 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
    10861 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
    10921 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
    10981 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
    11041 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
    11101 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
    11161 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
    11221 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
    11281 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
    11341 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
    11401 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
    11461 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
    11521 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
    11581 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
    11641 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
    11701 cagcagagcg cagataccaa atactgtctt tctagtgtag ccgtagttag gccaccactt
    11761 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
    11821 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
    11881 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
    11941 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
    12001 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
    12061 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
    12121 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
    12181 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
    12241 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
    12301 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
    12361 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt
    12421 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
    12481 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
    12541 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
    12601 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
    12661 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
    12721 tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt
    12781 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
    12841 tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc
    12901 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
    12961 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
    13021 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg
    13081 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
    13141 aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt
    13201 attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
    13261 gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt
    13321 gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc
    13381 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt
    13441 aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca
    13501 ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct
    13561 agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac
    13621 catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc
    13681 acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga
    13741 tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc
    13801 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
    13861 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
    13921 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
    13981 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
    14041 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
    14101 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
    14161 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
    14221 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac
    14281 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat
    14341 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg
    14401 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
    14461 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
    14521 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    14581 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    14641 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
    14701 taccctatta ccactgccaa ttacctagtg gtttcattta ctctaaacct gtgattcctc
    14761 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
