LOCUS Exported 7873 bp ds-DNA circular SYN 18-SEP-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7873) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 18, 2017 from SnapGene 4.0.5 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7873 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 135..441 /label=F1 rep_Origin /note="F1 rep_Origin" misc_feature 460..528 /label=LacZ-alpha CDS /note="LacZ-alpha CDS" misc_feature 599..616 /label=M13 Fwd /note="M13 Fwd" misc_feature 615..642 /label=T7 /note="T7" misc_feature 656..695 /label=5' micro ITR /note="5' micro ITR" misc_feature 1052..2654 /label=CAG promoter /note="CAG promoter" misc_feature 2721..3464 /label=rtTA-M2 /note="rtTA-M2" misc_feature 3502..4065 /label=IRES /note="IRES" misc_feature 4100..5134 /label=Hygro resistance /note="Hygro resistance" misc_feature 5370..5424 /label=Sv40 PolyA /note="Sv40 PolyA" misc_feature 5599..5665 /label=3' micro ITR PB /note="3' micro ITR PB" misc_feature 5683..5702 /label=T3 /note="T3" misc_feature 5720..5740 /label=M13 Rev /note="M13 Rev" misc_feature 5746..5768 /label=LacO /note="LacO" misc_feature 6108..6790 /label=ColE1 origin /note="ColE1 origin" misc_feature 6888..7547 /label=Amp resistance /note="Amp resistance" ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac 661 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt 721 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc 781 cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg 841 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat 901 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta 961 tagatatcgt cgacattgat tattgactag ctagttatta atagtaatca attacggggt 1021 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc 1081 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag 1141 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc 1201 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg 1261 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc 1321 agtacatcta cgtattagtc atcgctatta ccatgatggt cgaggtgagc cccacgttct 1381 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt 1441 aattattttg tgcagcgatg ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg 1501 ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg 1561 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc 1621 gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg 1681 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc 1741 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt 1801 ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg 1861 gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc 1921 ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg 1981 aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag 2041 gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc 2101 tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg 2161 ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg 2221 gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg 2281 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt 2341 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg 2401 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga 2461 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc 2521 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt 2581 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct 2641 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca 2701 aagaattcct cgaggccacc atgtctagac tggacaagag caaagtcata aacggcgctc 2761 tggaattact caatggagtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc 2821 tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg 2881 ccctgccaat cgagatgctg gacaggcatc atacccactt ctgccccctg gaaggcgagt 2941 catggcaaga ctttctgcgg aacaacgcca agtcattccg ctgtgctctc ctctcacatc 3001 gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg 3061 aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc 3121 tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag 3181 caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg agacaagcaa 3241 ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca 3301 tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccggccgac gcccttgacg 3361 attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt gatatgctgc 3421 ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgggtaagct cgagactagt 3481 tctagagcgg ccgcggatcc cgcccctctc cctccccccc ccctaacgtt actggccgaa 3541 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt 3601 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg 3661 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc 3721 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc 3781 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa 3841 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc 3901 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg 3961 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac 4021 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt 4081 gccacaaccc gtaccaaaga tggatagatc cggaaagcct gaactcaccg cgacgtctgt 4141 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg 4201 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa 4261 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc 4321 gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat 4381 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt 4441 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag 4501 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat 4561 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag 4621 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt 4681 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat 4741 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa 4801 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg 4861 gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct 4921 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg 4981 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg 5041 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg 5101 acgccccagc actcgtccga gggcaaagga atagccatgg cgatgcctgc ttgccgaata 5161 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 5221 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 5281 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 5341 tctatcgcct tcttgacgag ttcttctgag gggatcggca ataaaaagac agaataaaac 5401 gcacgggtgt tgggtcgttt gttcggatcc tttgttactt tatagaagaa attttgagtt 5461 tttgtttttt tttaataaat aaataaacat aaataaattg tttgttgaat ttattattag 5521 tatgtaagtg taaatataat aaaacttaat atctattcaa attaataaat aaacctcgat 5581 atacagaccg ataaaacaca tgcgtcaatt ttacgcatga ttatctttaa cgtacgtcac 5641 aatatgatta tctttctagg gttaaagagc tccagctttt gttcccttta gtgagggtta 5701 atttcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 5761 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 5821 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 5881 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 5941 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 6001 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 6061 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 6121 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 6181 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 6241 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 6301 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 6361 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 6421 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 6481 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 6541 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 6601 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 6661 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 6721 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 6781 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 6841 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 6901 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 6961 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 7021 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 7081 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 7141 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 7201 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 7261 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 7321 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 7381 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 7441 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 7501 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 7561 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 7621 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 7681 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 7741 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 7801 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 7861 cgaaaagtgc cac //