LOCUS       pTR47m4-GFP             4328 bp ds-DNA     circular     30-MAY-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator      complement(3202..3243)
                     /label="termination of bla transcript is slightly weakerthan opposite direction"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3153..3160
                     /label="Keep unique in all constructs, can be used toexchange antibiotic resistance marker or selectioncomponents"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1850..1854
                     /label="-10"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1786..1816
                     /label="spacer"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     RBS             1882..1896
                     /label="SD8 RBS"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             1897..2613
                     /label="sGFP"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(2680..2718)
                     /label="AB016"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      complement(955..1700)
                     /label="pSC101"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(1..951)
                     /label="pSC101 origin of replication protein"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     terminator      2628..2661
                     /label="BBa_B1002"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     terminator      complement(2663..2701)
                     /label="BBa_B1006"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1783..1818
                     /label="Fluff Region for Initial AP"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1761..1767
                     /label="nicking endonuclease site that generates overhang"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(3257..4117)
                     /label="from pUC19"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(3010..3026)
                     /label="USER linker"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1825..1830
                     /label="-35"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1775..1781
                     /label="Keep unique in all constructs, can be used toexchange f1 origin or selection components"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     promoter        complement(4118..4312)
                     /label="from pUC19"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    complement(3059..3077)
                     /label="tetR binding site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        complement(3077..3112)
                     /label="tet-regulated promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     modified_base   4313..4328
                     /label="USER junction"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     RBS             complement(2995..3008)
                     /label="Ringquist and Gold"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(2719..2994)
                     /label="frmR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1867..1896
                     /label="AB0596"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     RBS             1883..1896
                     /label="Ringquist and Gold"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3151..3167
                     /label="USER linker"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1867..1896
                     /label="TR066"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     terminator      1716..1760
                     /label="rrnB1 transcriptional terminator"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     modified_base   3244..3256
                     /label="USER junction"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2702..2718
                     /label="USER linker"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     protein_bind    complement(3113..3131)
                     /label="tetR binding site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1875..1879
                     /label="Keep unique in all constructs to swap out RBS"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1868..1875
                     /label="Keep unique in all constructs to switch outpromoter or RBS"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1867..1881
                     /label="USER linker"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    999..1221
                     /label="pSC101 origin"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3010..3062
                     /label="AB052"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3168..3201
                     /label="BBa_B1002"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1855..1866
                     /label="Fluff Region added to initial AP"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     terminator      complement(3168..3201)
                     /label="BBa_B1002"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    3244..3246
                     /label="add new stuff here?"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    1819..1854
                     /label="frm Promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     modified_base   1768..1782
                     /label="USER junction"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3168..3168
                     /label="insert other genes here"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    4314..4326
                     /label="Keep unique in all constructs, can be used toexchange antibiotic resistance markers or plasmid origins"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(3260..3919)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     modified_base   1701..1715
                     /label="USER junction"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(3089..3107)
                     /label="tetO1"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
        1 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag
       61 ccatgagaac gaaccattga gatcatgctt actttgcatg tcactcaaaa attttgcctc
      121 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt
      181 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg
      241 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa
      301 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa
      361 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt
      421 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt
      481 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc
      541 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg
      601 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt
      661 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat
      721 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat
      781 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat
      841 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc
      901 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa
      961 ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattact
     1021 agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact
     1081 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta
     1141 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga
     1201 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc
     1261 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac
     1321 cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga
     1381 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg
     1441 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag
     1501 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct
     1561 gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta
     1621 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca
     1681 ggcttacccg tcttactgtc aagaggacat ccggtcaaat aaaacgaaag gctcagtcga
     1741 aagactgggc ctttcgtttt gctgaggaga cttagggacc ctAAATTTTA TTCTCCAGTG
     1801 TTATATACTA TAGGGGGGTA TGCATTGACA TATAGCATAC aCtCCTATAG TATATGCGTG
     1861 CAGATAacct gcaggtgcag taaggaggaa aaaaaaATGA GCAAGGGCGA AGAGCTTTTT
     1921 ACCGGTGTTG TGCCGATTTT AGTAGAACTG GACGGAGACG TGAACGGTCA TAAGTTCTCT
     1981 GTTCGTGGCG AAGGAGAGGG AGATGCCACC AATGGTAAGC TGACCCTGAA GTTCATCTGT
     2041 ACCACCGGTA AGCTGCCCGT GCCTTGGCCG ACGCTGGTCA CAACGTTGAC GTATGGCGTC
     2101 CAATGCTTTT CACGCTATCC AGATCACATG AAACGCCACG ACTTTTTTAA AAGCGCAATG
     2161 CCTGAAGGTT ATGTGCAGGA ACGGACTATT AGCTTCAAAG ACGATGGGAC GTATAAGACC
     2221 CGCGCGGAAG TGAAATTTGA AGGCGATACC TTAGTTAACC GCATTGAATT AAAAGGTATC
     2281 GATTTCAAAG AGGATGGGAA TATCCTGGGG CACAAATTGG AATACAACTT TAATTCGCAC
     2341 AACGTATACA TTACAGCGGA TAAACAGAAA AATGGCATCA AAGCCAACTT TAAAATCCGT
     2401 CATAACGTAG AGGACGGTTC CGTGCAGCTG GCTGATCATT ACCAGCAGAA TACTCCGATT
     2461 GGCGATGGCC CCGTTCTGCT CCCGGATAAT CATTACCTGT CTACACAAAG CGTTCTTAGT
     2521 AAAGACCCAA ACGAGAAGCG TGACCATATG GTCCTGTTGG AATTCGTCAC GGCAGCGGGG
     2581 ATTACTCATG GCATGGATGA ACTCTATAAG TAAacggagc caatgtacgc aaaaaacccc
     2641 gcttcagcgg ggttttttcg ccaaaaaaaa ccccgccctg tcaggggcgg ggtttttttt
     2701 tagggcccta ctgactgtCT ATTTAAGATA GGCTCGAACC AGTTCAATAG TGTCGTCAAC
     2761 GGATTGGCTG ACTTCGCGGC TGTAGCAGTC ATTTCGGTCA AACGTTTCCC GGATATGGCT
     2821 TTCAAGCACT TCTGCCATCA GCCCATTAGC CGCGCCCCGA ACGGCAGCGA TCTGTTGGAG
     2881 TATGGCACGG CATTCGGCAT CACCCTCCAG CGACCGTTCC AGAGCATCAA TCTGCCCTCG
     2941 AATACGACGA ACTCGAGTAA GGACCTTTTT CTTCTCTTCC GGAGTACTGG GCATtttttt
     3001 ttcctcctta taagggggta gggggtctag atttttgtcg aactattcat ttcacttttc
     3061 tctatcactg atagggagtg gtaaaataac tctatcaatg atagagtgtc aatctctatc
     3121 actgataggg atcatctggc cattcgatgg acttaattaa cggcactgcg aaaaaacccc
     3181 gccgaagcgg ggttttttgc gaaatataat gaccctcttg ataacccaag agggcatttt
     3241 ttaatgccca tggcgtttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     3301 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     3361 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3421 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3481 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3541 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3601 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3661 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3721 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     3781 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     3841 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     3901 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     3961 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     4021 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     4081 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     4141 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     4201 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     4261 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc ttaggccaaa
     4321 taggccgt
//