LOCUS       pHS2_(V5-hANGPTL4)      6249 bp ds-DNA     circular     20-SEP-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        complement(6114..6212)
                     /label="bla promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      3922..4052
                     /label="SV40 polyA"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    923..1003
                     /label="Signal peptide"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    912..922
                     /label="pMD2"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             2141..2182
                     /label="V5 epitope"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    923..911
                     /label="pCDNA6/V5-His B"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        3298..3365
                     /label="EM7 promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        2914..3283
                     /label="SV40 early promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     rep_origin      complement(4435..5108)
                     /label="pUC origin"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     rep_origin      2481..2909
                     /label="f1 origin"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      2211..2435
                     /label="BGH polyA"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             3366..3764
                     /label="Bsd (R)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             923..2140
                     /label="hANGPTL4"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(5253..6113)
                     /label="Amp (R)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     promoter        232..819
                     /label="CMV promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     modified_base   2089..2089
                     /label=""
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        863..879
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccATGAGCGG TGCTCCGACG GCCGGGGCAG CCCTGATGCT
      961 CTGCGCCGCC ACCGCCGTGC TACTGAGCGC TCAGGGCGGA CCCGTGCAGT CCAAGTCGCC
     1021 GCGCTTTGCG TCCTGGGACG AGATGAATGT CCTGGCGCAC GGACTCCTGC AGCTCGGCCA
     1081 GGGGCTGCGC GAACACGCGG AGCGCACCCG CAGTCAGCTG AGCGCGCTGG AGCGGCGCCT
     1141 GAGCGCGTGC GGGTCCGCCT GTCAGGGAAC CGAGGGGTCC ACCGACCTCC CGTTAGCCCC
     1201 TGAGAGCCGG GTGGACCCTG AGGTCCTTCA CAGCCTGCAG ACACAACTCA AGGCTCAGAA
     1261 CAGCAGGATC CAGCAACTCT TCCACAAGGT GGCCCAGCAG CAGCGGCACC TGGAGAAGCA
     1321 GCACCTGCGA ATTCAGCATC TGCAAAGCCA GTTTGGCCTC CTGGACCACA AGCACCTAGA
     1381 CCATGAGGTG GCCAAGCCTG CCCGAAGAAA GAGGCTGCCC GAGATGGCCC AGCCAGTTGA
     1441 CCCGGCTCAC AATGTCAGCC GCCTGCACCG GCTGCCCAGG GATTGCCAGG AGCTGTTCCA
     1501 GGTTGGGGAG AGGCAGAGTG GACTATTTGA AATCCAGCCT CAGGGGTCTC CGCCATTTTT
     1561 GGTGAACTGC AAGATGACCT CAGATGGAGG CTGGACAGTA ATTCAGAGGC GCCACGATGG
     1621 CTCAGTGGAC TTCAACCGGC CCTGGGAAGC CTACAAGGCG GGGTTTGGGG ATCCCCACGG
     1681 CGAGTTCTGG CTGGGTCTGG AGAAGGTGCA TAGCATCACG GGGGACCGCA ACAGCCGCCT
     1741 GGCCGTGCAG CTGCGGGACT GGGATGGCAA CGCCGAGTTG CTGCAGTTCT CCGTGCACCT
     1801 GGGTGGCGAG GACACGGCCT ATAGCCTGCA GCTCACTGCA CCCGTGGCCG GCCAGCTGGG
     1861 CGCCACCACC GTCCCACCCA GCGGCCTCTC CGTACCCTTC TCCACTTGGG ACCAGGATCA
     1921 CGACCTCCGC AGGGACAAGA ACTGCGCCAA GAGCCTCTCT GGAGGCTGGT GGTTTGGCAC
     1981 CTGCAGCCAT TCCAACCTCA ACGGCCAGTA CTTCCGCTCC ATCCCACAGC AGCGGCAGAA
     2041 GCTTAAGAAG GGAATCTTCT GGAAGACCTG GCGGGGCCGC TACTACCCAC TGCAGGCCAC
     2101 CACCATGTTG ATCCAGCCCA TGGCAGCAGA GGCAGCCTCC ggtaagccta tccctaaccc
     2161 tctcctcggt ctcgattcta cgtgagttta aacccgctga tcagcctcga ctgtgccttc
     2221 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     2281 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     2341 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     2401 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     2461 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     2521 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     2581 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     2641 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     2701 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     2761 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     2821 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     2881 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     2941 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3001 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3061 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     3121 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     3181 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     3241 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
     3301 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
     3361 aaaccatggc caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta
     3421 caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg
     3481 acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac
     3541 tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga
     3601 tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg
     3661 atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg
     3721 ggattcgtga attgctgccc tctggttatg tgtgggaggg ctaagcactt cgtggccgag
     3781 gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     3841 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     3901 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     3961 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     4021 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     4081 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     4141 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     4201 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     4261 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     4321 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     4381 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     4441 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4501 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4561 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4621 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4681 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4741 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4801 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     4861 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     4921 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     4981 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     5041 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     5101 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     5161 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     5221 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     5281 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5341 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5401 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5461 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5521 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     5581 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     5641 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     5701 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     5761 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     5821 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     5881 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     5941 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     6001 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     6061 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     6121 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     6181 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     6241 cctgacgtc
//