LOCUS       pSG86:_P38U18C35T14_v10-2b        3111 bp    DNA     circular UNA 15-AUG-2017
DEFINITION  J23151-BCD7-LasR-T14 v10-2b.
ACCESSION   urn.local.shaobinguo.1m-824ffqc
VERSION     urn.local.shaobinguo.1m-824ffqc
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    complement(<1..>12)
                     /created_by="User"
                     /label="vector-attach-promoter"
     misc_feature    <1..43
                     /Original_Bases="CCTGTAGGATCGTACAGGTTTACGCAAGAAAATGGTTTGTT
                     ATAGTCG"
                     /label="CCTGTAGGATCGTACAGGTTTACGCAAGAAAATGGTTTGTTATAGTCG"
                     /note="Geneious type: Editing History Replacement"
     ligation        1..4
                     /label="Ligation"
     misc_feature    5..43
                     /Source="referenceToDocument:
                     urn:local:shaobinguo:32-5e389ro"
                     /label="Digestion of IA_P38 - Fragment 2 extraction
                     (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     ligation        44..47
                     /label="Ligation"
     misc_feature    48..131
                     /Source="referenceToDocument:
                     urn:local:shaobinguo:4y-5e38vsn"
                     /label="Digestion of IA_U18 - Fragment 2 extraction
                     (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    118..>135
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        132..135
                     /label="Ligation"
     misc_feature    132..>859
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     CDS             133..855
                     /created_by="User"
                     /label="lasR"
     misc_feature    136..855
                     /Source="referenceToDocument:
                     urn:local:shaobinguo:1i-5e389gr"
                     /label="Digestion of IA_C35 - Fragment 2 extraction
                     (concatenated sequence 3)"
                     /note="Geneious type: Concatenated sequence"
     ligation        856..859
                     /label="Ligation"
     misc_feature    <856..861
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    860..922
                     /Source="referenceToDocument:
                     urn:local:shaobinguo:11-5bz3hbf"
                     /label="Digestion of IA_T14 by BsaI - Fragment 2
                     extraction (concatenated sequence 4)"
                     /note="Geneious type: Concatenated sequence"
     terminator      862..914
                     /created_by="User"
                     /modified_by="User"
                     /label="ECK120033736"
     misc_feature    915..>926
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        923..926
                     /label="Ligation"
     misc_feature    <923..938
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    927..3111
                     /Source="referenceToDocument:
                     urn:local:shaobinguo:6q-5e38w6o"
                     /label="Digestion of IA_v10-2b - Fragment 2 extraction
                     (concatenated sequence 5)"
                     /note="Geneious type: Concatenated sequence"
     ORF             <939..1703
                     /Genetic_code="Standard"
                     /Reading_frame=2
                     /created_by="ORF Finder"
                     /label="ORF 2 (frame 2)"
     conflict        939..955
                     /created_by="User"
                     /label="vB2 binding sitre"
     misc_feature    1044..1700
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /label="CmR"
     misc_feature    2113..2953
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /label="Ori-p15A (Chris)"
     misc_feature    2376..2921
                     /ApEinfo_fwdcolor="#0080c0"
                     /ApEinfo_revcolor="#80ff00"
                     /label="p15A ORI"
     conflict        3080..3099
                     /created_by="User"
                     /label="vB1 binding site"
     misc_feature    3100..>4
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
ORIGIN      
        1 gcattgctgt tcttgatggc tagctcagtc ctaggtacaa tgcaagcggg cccaagttca
       61 cttaaaaagg agatcaacaa tgaaagcaat tttcgtactg aaacatctta atcatgctgg
      121 ggagggtttc taatggcctt ggttgacggt tttcttgagc tggaacgctc aagtggaaaa
      181 ttggagtgga gcgccatcct ccagaagatg gcgagcgacc ttggattctc gaagatcctg
      241 ttcggcctgt tgcctaagga cagccaggac tacgagaacg ccttcatcgt cggcaactac
      301 ccggccgcct ggcgcgagca ttacgaccgg gctggctacg cgcgggtcga cccgacggtc
      361 agtcactgta cccagagcgt actgccgatt ttctgggaac cgtccatcta ccagacgcga
      421 aagcagcacg agttcttcga ggaagcctcg gccgccggcc tggtgtatgg gctgaccatg
      481 ccgctgcatg gtgctcgcgg cgaactcggc gcgctgagcc tcagcgtgga agcggaaaac
      541 cgggccgagg ccaaccgttt catagagtcg gtcctgccga ccctgtggat gctcaaggac
      601 tacgcactgc aaagcggtgc cggactggcc ttcgaacatc cggtcagcaa accggtggtt
      661 ctgaccagcc gggagaagga agtgttgcag tggtgcgcca tcggcaagac cagttgggag
      721 atatcggtta tctgcaactg ctcggaagcc aatgtgaact tccatatggg aaatattcgg
      781 cggaagttcg gtgtgacctc ccgccgcgta gcggccatta tggccgttaa tttgggtctt
      841 attactctct aataatgaac taacgcatga gaaagccccc ggaagatcac cttccggggg
      901 cttttttatt gcgcccttga gagtcgatgt ttgtcttctt gatcgggcac gtaagaggtt
      961 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
     1021 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
     1081 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
     1141 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
     1201 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
     1261 ttcgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     1321 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
     1381 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     1441 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
     1501 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     1561 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     1621 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     1681 atgagtggca gggcggggcg taatttgata tcgagctcgc ttggactcct gttgatagat
     1741 ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg
     1801 ttttttattg gtgagaatcc aagcctcggt gagaatccaa gcctcgatca acgtctcatt
     1861 ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg gacaccagga tttatttatt
     1921 ctgcgaagtg atcttccgtc acaggtattt attcggcgca aagtgcgtcg ggtgatgctg
     1981 ccaacttact gatttagtgt atgatggtgt ttttgaggtg ctccagtggc ttctgtttct
     2041 atcagctgtc cctcctgttc agctactgac ggggtggtgc gtaacggcaa aagcaccgcc
     2101 ggacatcagc ggtagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca
     2161 gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg
     2221 atacaggata tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     2281 gcgagcggaa atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact
     2341 taacagggaa gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac
     2401 aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga
     2461 taccaggcgt ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt
     2521 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc
     2581 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg
     2641 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc
     2701 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt
     2761 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt
     2821 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt
     2881 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc
     2941 agataaaata tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca
     3001 gccccatacg atataagttg taattctcat gtttgacagc ttatcatcga taagcttccg
     3061 atggcgcgcc gagaggcttt acactttatg cttccggctg aagacaaggc a
//
