LOCUS       pSG69:_pOR21PR-UTR1-KcsAKv1.3-His-deGFP-T500        3804 bp    DNA     circular UNA 06-JUN-2016
DEFINITION  prep 0602 is 100% correct.
ACCESSION   urn.local.shaobinguo.3h-824gzxj
VERSION     urn.local.shaobinguo.3h-824gzxj
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     source          order(1..>34,<1107..3804)
                     /dnas_title="pBEST-OR2-OR1-Pr-UTR1-deGFP-T500"
                     /label="source"
     misc_feature    1..33
                     /note="UTR1"
                     /label="UTR1"
     misc_feature    35
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_difference <35..56
                     /created_by="User"
                     /label="Oligo_78f"
     ligation        35..38
                     /label="Ligation"
     CDS             36..>530
                     /vntifkey=84
                     /modified_by="User"
                     /label="KcsA-Kv1.3"
     source          <36..>38
                     /dnas_title="pBEST-OR2-OR1-Pr-UTR1-deGFP-T500"
                     /label="source"
     source          <36..>530
                     /clone_id=3884304
                     /organism="Homo sapiens"
                     /requester="rfachini"
                     /clone="pAMG21-MDG-coKcsA/KcsA(Chi IV) #-C6"
                     /vntifkey=98
                     /label="source Homo sapiens"
     misc_feature    39..548
                     /Source="referenceToDocument: urn:local:.:3l-824gzyy"
                     /label="Digestion of GGA Kv1.3 - Fragment 2 extraction
                     (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     primer_bind     164..181
                     /Sequence="CGTACTGCTTGCTGGTTC"
                     /Hairpin_Tm="None"
                     /Tm="56.1"
                     /Self_Dimer_Tm="None"
                     /%GC="55.6"
                     /modified_by="User"
                     /label="Oligo_81f"
     misc_feature    263
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    complement(333..349)
                     /Sequence="ATGCCAGCCACCATAAC"
                     /Hairpin_Tm="None"
                     /Tm="54.3"
                     /Self_Dimer_Tm="None"
                     /%GC="52.9"
                     /modified_by="User"
                     /label="Oligo_81r"
                     /note="Geneious type: primer_bind_reverse"
     misc_difference complement(512..>552)
                     /created_by="User"
                     /modified_by="User"
                     /label="Oligo_85r"
     CDS             <531..>548
                     /created_by="User"
                     /modified_by="User"
                     /label="His Tag"
     misc_feature    531..>552
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        549..552
                     /label="Ligation"
     misc_feature    <549..552
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    553..34
                     /label="Digestion of deGFP-666p-backbone - Fragment 2
                     (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    623..640
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        637..640
                     /label="Ligation"
     source          <641..>1110
                     /dnas_title="pBEST-OR2-OR1-Pr-UTR1-deGFP-T500"
                     /label="source"
     misc_feature    <641..>1110
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /label="deGFP"
     ANNOTATION      1107..1123
                     /created_by="User"
                     /label="use me"
     ligation        1107..1110
                     /label="Ligation"
     misc_feature    <1107..1315
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /label="deGFP"
     terminator      1322..1351
                     /note="T500"
                     /label="T500 terminator"
     conflict        1388..1412
                     /created_by="User"
                     /label="area for IA_v1, v2"
     conflict        1391..1405
                     /created_by="User"
                     /label="area for IB_v1, v2"
     modified_base   1440
                     /created_by="User"
                     /label="c->g"
     misc_feature    1440
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     rep_origin      complement(1645..2327)
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /label="ColE1 origin"
     misc_feature    1857^1858
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    1859
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    2104
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     CDS             complement(2422..3282)
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /modified_by="User"
                     /label="AmpR"
     modified_base   2563
                     /created_by="User"
                     /label="a->t"
     misc_feature    2563
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2813
                     /created_by="User"
                     /label="A->G unique mut. not inherited from 21p"
     misc_feature    2813
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3039
                     /created_by="User"
                     /label="nonsilenced mutation Ile -> Val"
     misc_feature    3039
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     modified_base   3482
                     /created_by="User"
                     /label="c->g"
     misc_feature    3482
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3562^3563
                     /Original_Bases="ACCGGATGCTGCAATTCAGAGCG"
                     /label="ACCGGATGCTGCAATTCAGAGCG"
                     /note="Geneious type: Editing History Deletion"
     modified_base   3584
                     /created_by="User"
                     /label="a->t"
     misc_feature    3584
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     modified_base   3622
                     /created_by="User"
                     /modified_by="User"
                     /label="a->t"
     misc_feature    3622
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     conflict        3688..3704
                     /created_by="User"
                     /label="area for IA_vb1, vb2"
     conflict        3690..3707
                     /created_by="User"
                     /label="area for IA_vA1, IA_vA2"
     misc_feature    3744..3798
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /label="OR2-OR1-Pr"
     promoter        3763..3768
                     /created_by="User"
                     /label="-35"
     promoter        3786..3791
                     /created_by="User"
                     /modified_by="User"
                     /label="-10"
     ANNOTATION      complement(3801..>34)
                     /created_by="User"
                     /label="use me"
ORIGIN      
        1 aataattttg tttaacttta agaaggagat atacaatgga cgaagttgat ggtccgccaa
       61 tgttgtccgg tctgcttgct cgtctggtta agttgcttct gggccgtcac ggttctgcgc
      121 tgcactggcg tgctgcaggc gctgccaccg tccttctggt gatcgtactg cttgctggtt
      181 cctacttggc ggttctggcg gaagcggatg atccgaccag cggctttagc agcattccgg
      241 acgccttgtg gtggtctgtc gataccgcta ctaccgtggg ttacggcgac ttgtatccgg
      301 tgaccctttg gggtcgtctg gtagcggttg tcgttatggt ggctggcatc acctccttcg
      361 gtctggtaac tgctgccctt gctacttggt ttgtaggccg cgaacaagag cgtcgtggtc
      421 atttcgttcg tcactctgaa aaagcggctg aggaggcgta cacccgtact acccgtgctc
      481 tgcacgagcg ctttgaccgt ctggaacgta tgcttgacga taaccgtcgt catcatcatc
      541 atcatcattg aagggcccaa gttcacttaa aaaggagatc aacaatgaaa gcaattttcg
      601 tactgaaaca tcttaatcat gcaatggagg ctttctaatg gagcttttca ctggcgttgt
      661 tcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
      721 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      781 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
      841 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
      901 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
      961 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1021 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1081 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     1141 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1201 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     1261 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tctaactcga
     1321 gcaaagcccg ccgaaaggcg ggcttttctg tgtcgaccga tgcccttgag agccttcaac
     1381 ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtg
     1441 ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac
     1501 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1561 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1621 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1681 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1741 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1801 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1861 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1921 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1981 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2041 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2101 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2161 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2221 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2281 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2341 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     2401 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     2461 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     2521 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgtgacccac gctcaccggc
     2581 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     2641 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     2701 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     2761 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttgcatgatc
     2821 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     2881 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     2941 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3001 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
     3061 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     3121 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     3181 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     3241 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     3301 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     3361 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     3421 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     3481 tgttcaagaa ttctggcgaa tcctctgacc agccagaaaa cgacctttct gtggtgaaac
     3541 cggatgctgc aattcagagc gccagcaagt gggggacagc agatgacctg accgccgcag
     3601 agtggatgtt tgacatggtg atgactatcg caccatcagc cagaaaaccg aattttgctg
     3661 ggtgggctaa cgatatccgc ctgatgcgtg aacgtgacgg acgtaaccac cgcgacatgt
     3721 gtgtgctgtt ccgctgggca tgctgagcta acaccgtgcg tgttgacaat tttacctctg
     3781 gcggtgataa tggttgcagc tagc
//
