LOCUS       pSG73:_b1AR-ts_plasmid        4241 bp    DNA     circular UNA 17-JUN-2016
DEFINITION  prep 0705 No.2 had some overlapping sequence with template but very
            noisy. maybe because of the low conc. of plasmid.
ACCESSION   urn.local.shaobinguo.2i-824gupe
VERSION     urn.local.shaobinguo.2i-824gupe
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    <1..759
                     /Original_Bases="TGCGTAAAGGCGAAGAGCTGTTCACTGGTGTCGTCCCTATT
                     CTGGTGGAACTGGATGGTGATGTCAACGGTCATAAGTTTTCCGTGCGTGGCGAGGGTG
                     AAGGTGACGCAACTAATGGTAAACTGACGCTGAAGTTCATCTGTACTACTGGTAAACT
                     GCCGGTACCTTGGCCGACTCTGGTAACGACGCTGACTTATGGTGTTCAGTGCTTTGCT
                     CGTTATCCGGACCATATGAAGCAGCATGACTTCTTCAAGTCCGCCATGCCGGAAGGCT
                     ATGTGCAGGAACGCACGATTTCCTTTAAGGATGACGGCACGTACAAAACGCGTGCGGA
                     AGTGAAATTTGAAGGCGATACCCTGGTAAACCGCATTGAGCTGAAAGGCATTGACTTT
                     AAAGAAGACGGCAATATCCTGGGCCATAAGCTGGAATACAATTTTAACAGCCACAATG
                     TTTACATCACCGCCGATAAACAAAAAAATGGCATTAAAGCGAATTTTAAAATTCGCCA
                     CAACGTGGAGGATGGCAGCGTGCAGCTGGCTGATCACTACCAGCAAAACACTCCAATC
                     GGTGATGGTCCTGTTCTGCTGCCAGACAATCACTATCTGAGCACGCAAAGCGTTCTGT
                     CTAAAGATCCGAACGAGAAACGCGATCATATGGTTCTGCTGGAGTTCGTAACCGCAGC
                     GGGCATCACGCATGGTATGGATGAACTGTACAAATGA"
                     /label="TGCGTAAAGGCGAAGAGCTGTTCACTGGTGTCGTCCCTATTCTGGTGGAA
                     CTGGATGGTGATGTCAACGGTCATAAGTTTTCCGTGCGTGGCGAGGGTGAAGGTGACG
                     CAACTAATGGTAAACTGACGCTGAAGTTCATCTGTACTACTGGTAAACTGCCGGTACC
                     TTGGCCGACTCTGGTAACGACGCTGACTTATGGTGTTCAGTGCTTTGCTCGTTATCCG
                     GACCATATGAAGCAGCATGACTTCTTCAAGTCCGCCATGCCGGAAGGCTATGTGCAGG
                     AACGCACGATTTCCTTTAAGGATGACGGCACGTACAAAACGCGTGCGGAAGTGAAATT
                     TGAAGGCGATACCCTGGTAAACCGCATTGAGCTGAAAGGCATTGACTTTAAAGAAGAC
                     GGCAATATCCTGGGCCATAAGCTGGAATACAATTTTAACAGCCACAATGTTTACATCA
                     CCGCCGATAAACAAAAAAATGGCATTAAAGCGAATTTTAAAATTCGCCACAACGTGGA
                     GGATGGCAGCGTGCAGCTGGCTGATCACTACCAGCAAAACACTCCAATCGGTGATGGT
                     CCTGTTCTGCTGCCAGACAATCACTATCTGAGCACGCAAAGCGTTCTGTCTAAAGATC
                     CGAACGAGAAACGCGATCATATGGTTCTGCTGGAGTTCGTAACCGCAGCGGGCATCAC
                     GCATGGTATGGATGAACTGTACAAATGA"
                     /note="Geneious type: Editing History Replacement"
     ligation        1..4
                     /label="Ligation"
     misc_difference <5..27
                     /created_by="User"
                     /label="Linker"
     misc_feature    5..759
                     /Source="referenceToDocument: urn:local:.:2z-824gutg"
                     /label="Digestion of Amgen sfGFP - Fragment 2 extraction
                     (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    <5..>27
                     /Source="referenceToDocument: urn:local:.:30-824gutg"
                     /label="deGFP (21) (concatenated sequence 3)"
                     /note="Geneious type: Concatenated sequence"
     CDS             28..>741
                     /created_by="User"
                     /label="sfGFP BBa Safe"
     polyA_site      742..759
                     /created_by="User"
                     /label="His tag"
     misc_feature    <760..>3290
                     /Original_Bases="TGTGTTAGAAGGTCGAGTCACCATTAGCCAGGTCTCAAAGC
                     GGGCCCAAGTTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAAACA
                     TCTTAATCATGC"
                     /label="TGTGTTAGAAGGTCGAGTCACCATTAGCCAGGTCTCAAAGCGGGCCCAAG
                     TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAAACATCTTAATCA
                     TGC"
                     /note="Geneious type: Editing History Replacement"
     ligation        760..763
                     /label="Ligation"
     source          <764..3252
                     /dnas_title="pBEST-OR2-OR1-Pr-UTR1-deGFP-T500"
                     /label="source"
     misc_feature    764..3286
                     /Source="referenceToDocument: urn:local:.:2x-824gutg"
                     /label="Digestion of Amgen pSG66 backbone - Fragment 2
                     extraction (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    <764..>3252
                     /label="Digestion of piece 1 ext by BsaI - Fragment 2
                     (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     PCR_product     join(3164..3252,<764..829)
                     /pair="Pair 1"
                     /set="800bp_amp"
                     /current=1
                     /score="0.000"
                     /modified_by="User"
                     /label="868bp-linear fragment"
     PCR_product     join(2940..3252,<764..1056)
                     /pair="Pair 3"
                     /set="1200bp_amp"
                     /current=0
                     /score="0.264"
                     /modified_by="User"
                     /label="1319bp-linear fragment"
     PCR_product     join(2696..3252,<764..1283)
                     /pair="Pair 1"
                     /set="1600bp_amp"
                     /current=1
                     /score="1.000"
                     /modified_by="User"
                     /label="1768bp-linear fragment"
     misc_feature    complement(766..804)
                     /NEB_Tm=91
                     /created_by="User"
                     /label="ZS3034rb"
                     /note="Geneious type: primer_bind_reverse"
     terminator      770..799
                     /note="T500"
                     /label="T500 terminator"
     misc_feature    complement(782..799)
                     /NEB_Tm=64
                     /created_by="User"
                     /label="ZS3033ra"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(787..804)
                     /NEB_Tm=65
                     /created_by="User"
                     /label="ZS3034ra"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    800..815
                     /created_by="User"
                     /label="ZS30218r"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(807..829)
                     /dnas_title="GGTTGAAGGCTCTCAAGGGCATC"
                     /primer="GGTTGAAGGCTCTCAAGGGCATC"
                     /set="800bp_amp"
                     /pair="Pair 1"
                     /current=1
                     /modified_by="User"
                     /label="ZS2011r"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(807..824)
                     /NEB_Tm=62
                     /created_by="User"
                     /label="ZS3035r"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     814..833
                     /NEB_Tm=64
                     /created_by="User"
                     /modified_by="User"
                     /label="ZS2101f"
     misc_feature    complement(824..843)
                     /NEB_Tm=64
                     /created_by="User"
                     /label="ZS21111r"
                     /note="Geneious type: primer_bind_reverse"
     conflict        836..860
                     /created_by="User"
                     /label="area for IA_v1, v2"
     conflict        839..853
                     /created_by="User"
                     /label="area for IB_v1, v2"
     primer_bind     848..867
                     /created_by="primer3"
                     /%GC="70.0"
                     /Tm="59.97"
                     /Hairpin="4.0"
                     /Primer-Dimer="2.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Sequence="GGGCGCGGGGCATGACTATC"
                     /annotation_group="PrimerPlugin
                     Groupd15da267-d8a2-4186-95fc-cf70491eedb3_urn:local:Zachar
                     y:1339793905171.9"
                     /label="4th forward primer"
     misc_feature    complement(873..899)
                     /NEB_Tm=64
                     /created_by="User"
                     /label="ZS3036r"
                     /note="Geneious type: primer_bind_reverse"
     modified_base   888
                     /created_by="User"
                     /label="c->g"
     misc_difference <928..>949
                     /created_by="User"
                     /label
     primer_bind     1003..1022
                     /created_by="User"
                     /label="30424b"
     misc_feature    complement(1032..1049)
                     /NEB_Tm=60
                     /created_by="User"
                     /label="ZS3037r"
                     /note="Geneious type: primer_bind_reverse"
     PCR_primer      complement(1033..1056)
                     /dnas_title="CACATGTTCTTTCCTGCGTTATCC"
                     /primer="CACATGTTCTTTCCTGCGTTATCC"
                     /set="1200bp_amp"
                     /pair="Pair 3"
                     /current=0
                     /label="PCR primer"
     misc_feature    complement(1074..1093)
                     /created_by="User"
                     /label="30425b"
                     /note="Geneious type: primer_bind_reverse"
     rep_origin      complement(1093..1775)
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /label="ColE1 origin"
     PCR_primer      complement(1260..1283)
                     /dnas_title="AAGGGAGAAAGGCGGACAGGTATC"
                     /primer="AAGGGAGAAAGGCGGACAGGTATC"
                     /set="1600bp_amp"
                     /pair="Pair 1"
                     /current=1
                     /label="PCR primer"
     misc_feature    complement(1285..1299)
                     /NEB_tm=67
                     /created_by="User"
                     /label="ZS3038r"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     1499..1518
                     /created_by="User"
                     /label="30424c"
     misc_feature    complement(1578..1597)
                     /created_by="User"
                     /label="30425c"
                     /note="Geneious type: primer_bind_reverse"
     CDS             complement(1870..2730)
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /modified_by="User"
                     /label="AmpR"
     primer_bind     2000..2018
                     /created_by="User"
                     /label="30424d"
     modified_base   2011
                     /created_by="User"
                     /label="a->t"
     misc_feature    complement(2080..2099)
                     /created_by="User"
                     /label="30425d"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    2261
                     /created_by="User"
                     /label="A->G unique mut. not inherited from 21p"
     primer_bind     2464..2483
                     /created_by="primer3"
                     /%GC="65.0"
                     /Tm="59.97"
                     /Hairpin="5.0"
                     /Primer-Dimer="1.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Sequence="GAGTTGCTCTTGCCCGGCGT"
                     /annotation_group="PrimerPlugin
                     Group35e94697-015b-4ba9-a196-d1b75a060cac_urn:local:Zachar
                     y:1339793905171.9"
                     /label="3rd forward primer"
     primer_bind     2465..2484
                     /created_by="primer3"
                     /%GC="65.0"
                     /Tm="59.97"
                     /Hairpin="5.0"
                     /Primer-Dimer="2.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Sequence="AGTTGCTCTTGCCCGGCGTC"
                     /annotation_group="PrimerPlugin
                     Group2b969374-cce2-477b-82d4-77ad94a045e5_urn:local:Zachar
                     y:1339793905171.9"
                     /label="2nd forward primer"
     misc_feature    2487
                     /created_by="User"
                     /label="nonsilenced mutation Ile -> Val"
     primer_bind     2501..2520
                     /created_by="User"
                     /label="30424e"
     misc_feature    complement(2576..2595)
                     /created_by="User"
                     /label="30425e"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     2692..2709
                     /NEB_Tm=63
                     /created_by="User"
                     /label="ZS3038f"
     PCR_primer      2696..2718
                     /dnas_title="GGAATAAGGGCGACACGGAAATG"
                     /primer="GGAATAAGGGCGACACGGAAATG"
                     /set="1600bp_amp"
                     /pair="Pair 1"
                     /current=1
                     /label="PCR primer"
     modified_base   2930
                     /created_by="User"
                     /label="c->g"
     PCR_primer      2940..2958
                     /dnas_title="TCTGGCGAATCCTCTGACC"
                     /primer="TCTGGCGAATCCTCTGACC"
                     /set="1200bp_amp"
                     /pair="Pair 3"
                     /current=0
                     /label="PCR primer"
     primer_bind     2942..2958
                     /NEB_tm=63
                     /created_by="User"
                     /label="ZS3037f"
     primer_bind     3000..3018
                     /created_by="User"
                     /label="30424f"
     modified_base   3032
                     /created_by="User"
                     /label="a->t"
     primer_bind     3062..3081
                     /NEB_Tm=61
                     /created_by="User"
                     /modified_by="User"
                     /label="ZS21111f"
     modified_base   3070
                     /created_by="User"
                     /modified_by="User"
                     /label="a->t"
     misc_feature    complement(3082..3101)
                     /neb_tm=64
                     /designed_by_primer3
                     /created_by="User"
                     /label="ZS21110r"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3082..3101)
                     /created_by="User"
                     /label="30425f"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     3092..3109
                     /NEb_Tm=60
                     /created_by="User"
                     /label="ZS3036f"
     conflict        3136..3152
                     /created_by="User"
                     /label="area for IA_vb1, vb2"
     conflict        3138..3155
                     /created_by="User"
                     /label="area for IA_vA1, IA_vA2"
     primer_bind     3164..3184
                     /dnas_title="CATGTGTGTGCTGTTCCGCTG"
                     /primer="CATGTGTGTGCTGTTCCGCTG"
                     /set="800bp_amp"
                     /pair="Pair 1"
                     /current=1
                     /modified_by="User"
                     /label="ZS2011f"
     primer_bind     3167..3183
                     /NEB_Tm=61
                     /created_by="User"
                     /label="ZS3035f"
     misc_feature    complement(3174..3189)
                     /created_by="User"
                     /label="ZS30218f"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     3187..3204
                     /NEB_Tm_62
                     /created_by="User"
                     /label="ZS3034f"
     promoter        3192..3246
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /modified_by="User"
                     /label="OR2-OR1-Pr"
     primer_bind     3192..3209
                     /created_by="User"
                     /NEB_tm=63
                     /modified_by="User"
                     /label="ZS3033f"
     promoter        3211..3216
                     /created_by="User"
                     /label="-35"
     promoter        3234..3239
                     /created_by="User"
                     /modified_by="User"
                     /label="-10"
     ANNOTATION      complement(3249..>3252)
                     /created_by="User"
                     /label="use me"
     ANNOTATION      complement(<3253..>3286)
                     /created_by="User"
                     /label="use me"
     source          3253..>3286
                     /dnas_title="pBEST-OR2-OR1-Pr-UTR1-deGFP-T500"
                     /label="source"
     polyA_site      3253..3285
                     /note="UTR1"
                     /modified_by="User"
                     /label="UTR1"
     misc_feature    <3253..3286
                     /label="Digestion of piece 1 ext by BsaI - Fragment 2
                     (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     PCR_product     3253..>3286
                     /pair="Pair 1"
                     /set="800bp_amp"
                     /current=1
                     /score="0.000"
                     /modified_by="User"
                     /label="868bp-linear fragment"
     PCR_product     3253..>3286
                     /pair="Pair 3"
                     /set="1200bp_amp"
                     /current=0
                     /score="0.264"
                     /modified_by="User"
                     /label="1319bp-linear fragment"
     PCR_product     3253..>3286
                     /pair="Pair 1"
                     /set="1600bp_amp"
                     /current=1
                     /score="1.000"
                     /modified_by="User"
                     /label="1768bp-linear fragment"
     primer_bind     3253..3282
                     /NEB_Tm=60
                     /created_by="User"
                     /modified_by="User"
                     /label="ZS3022f"
     misc_feature    3287
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        3287..3290
                     /label="Ligation"
     CDS             3288..4214
                     /created_by="User"
                     /label="b1AR-ts"
     misc_feature    3291..4241
                     /Source="referenceToDocument: urn:local:.:2y-824gutg"
                     /label="Digestion of GGA b1AR-ts - Fragment 2 extraction
                     (concatenated sequence 3)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    4215..>4
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_difference 4215..>4241
                     /created_by="User"
                     /label="Linker"
ORIGIN      
        1 gcattgtact tccagtctgg tggtgctatg cgtaaaggag aagaactttt cactggtgtc
       61 gtccctattc tggtggaact ggatggtgat gtcaacggtc ataagttttc cgtgcgtggc
      121 gagggtgaag gtgacgcaac taatggtaaa ctgacgctga agttcatctg tactactggt
      181 aaactgccgg taccttggcc gactctggta acgacgctga cttatggtgt tcagtgcttt
      241 gctcgttatc cggaccatat gaagcagcat gacttcttca agtccgccat gccggaaggc
      301 tatgtgcagg aacgcacgat ttcctttaag gatgacggca cgtacaaaac gcgtgcggaa
      361 gtgaaatttg aaggcgatac cctggtaaac cgcattgagc tgaaaggcat tgactttaaa
      421 gaagatggca atatcctggg ccataagctg gaatacaatt ttaacagcca caatgtttac
      481 atcaccgccg ataaacaaaa aaatggcatt aaagcgaatt ttaaaattcg ccacaacgtg
      541 gaggatggca gcgtgcagct ggctgatcac taccagcaaa acactccaat cggtgatggt
      601 cctgttctgc tgccagacaa tcactatctg agcacgcaaa gcgttctgtc taaagatccg
      661 aacgagaaac gcgatcatat ggttctgctg gagttcgtaa ccgcagcggg catcacgcat
      721 ggtatggatg aactgtacaa acatcatcat catcatcatt gaactcgagc aaagcccgcc
      781 gaaaggcggg cttttctgtg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc
      841 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtgtt ctttatcatg
      901 caactcgtag gacaggtgcc ggcagctctc ttccgcttcc tcgctcactt actcgctgcg
      961 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1021 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1081 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1141 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1201 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1261 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1321 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1381 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1441 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1501 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     1561 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     1621 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1681 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     1741 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     1801 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     1861 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     1921 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     1981 atctggcccc agtgctgcaa tgataccgcg tgacccacgc tcaccggctc cagatttatc
     2041 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     2101 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     2161 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     2221 ggcttcattc agctccggtt cccaacgatc aaggcgagtt gcatgatccc ccatgttgtg
     2281 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     2341 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     2401 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     2461 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt
     2521 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     2581 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     2641 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     2701 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     2761 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     2821 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     2881 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtg ttcaagaatt
     2941 ctggcgaatc ctctgaccag ccagaaaacg acctttctgt ggtgaaaccg gatgctgcaa
     3001 ttcagagcgc cagcaagtgg gggacagcag atgacctgac cgccgcagag tggatgtttg
     3061 acatggtgat gactatcgca ccatcagcca gaaaaccgaa ttttgctggg tgggctaacg
     3121 atatccgcct gatgcgtgaa cgtgacggac gtaaccaccg cgacatgtgt gtgctgttcc
     3181 gctgggcatg ctgagctaac accgtgcgtg ttgacaattt tacctctggc ggtgataatg
     3241 gttgcagcta gcaataattt tgtttaactt taagaaggag atatacaatg ggtgcagagc
     3301 ttttatccca gcaatgggag gctggtatgt ccttacttat ggctcttgtc gtgttgctga
     3361 ttgtggccgg gaacgtactt gtgatcgctg cgattgggag cacccaacgc cttcagacac
     3421 tgacaaacct gttcattaca tcgttggctt gcgctgactt agtcgtaggg ctgctggtag
     3481 tcccatttgg agcaacatta gtagtacgcg gaacatggtt gtggggctcc tttctttgtg
     3541 aattgtggac atcattagat gttttatgcg taacggcatc gattgaaaca ttatgtgtaa
     3601 ttgctattga ccgctacctt gcgattacgt ccccttttcg ctaccagtca ctgatgaccc
     3661 gtgctcgcgc aaaagttatt atctgtactg tttgggctat ctctgcgctg gtcagtttct
     3721 tgccgatcat gatgcactgg tggcgcgatg aagacccgca ggccctgaag tgttaccaag
     3781 atccgggttg ttgcgatttt gtgacaaatc gtgcatacgc aatcgccagc tcgattatct
     3841 cgttttatat tccattgtta atcatgatct ttgttgcgtt gcgcgtttac cgcgaggcga
     3901 aagaacagat ccgcaaaatt gaccgtgcga gcaaacgtaa gacatcacgt gtaatgttaa
     3961 tgcgtgagca taaagcctta aagacgttag gaatcattat gggtgtgttc actttatgct
     4021 ggttgccctt cttcctggta aacatcgtaa acgtatttaa tcgtgatttg gttccggact
     4081 ggctgtttgt tgcctttaat tggttaggat acgcgaattc cgcaatgaac cccatcattt
     4141 actgccgcag tcccgacttc cgtaaagcgt tcaaacgctt gttagccttt cctcgcaaag
     4201 ccgatcgtcg cctgggtgaa aacctgtact tccagtctgg t
//
