LOCUS       pSG74:_b2AR_plasmid        4553 bp    DNA     circular UNA 15-JUL-2016
DEFINITION  prep 0705 No.3 sequenced parts were 100% correct.
ACCESSION   urn.local.shaobinguo.2j-824gupe
VERSION     urn.local.shaobinguo.2j-824gupe
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    <1..759
                     /Original_Bases="TGCGTAAAGGCGAAGAGCTGTTCACTGGTGTCGTCCCTATT
                     CTGGTGGAACTGGATGGTGATGTCAACGGTCATAAGTTTTCCGTGCGTGGCGAGGGTG
                     AAGGTGACGCAACTAATGGTAAACTGACGCTGAAGTTCATCTGTACTACTGGTAAACT
                     GCCGGTACCTTGGCCGACTCTGGTAACGACGCTGACTTATGGTGTTCAGTGCTTTGCT
                     CGTTATCCGGACCATATGAAGCAGCATGACTTCTTCAAGTCCGCCATGCCGGAAGGCT
                     ATGTGCAGGAACGCACGATTTCCTTTAAGGATGACGGCACGTACAAAACGCGTGCGGA
                     AGTGAAATTTGAAGGCGATACCCTGGTAAACCGCATTGAGCTGAAAGGCATTGACTTT
                     AAAGAAGACGGCAATATCCTGGGCCATAAGCTGGAATACAATTTTAACAGCCACAATG
                     TTTACATCACCGCCGATAAACAAAAAAATGGCATTAAAGCGAATTTTAAAATTCGCCA
                     CAACGTGGAGGATGGCAGCGTGCAGCTGGCTGATCACTACCAGCAAAACACTCCAATC
                     GGTGATGGTCCTGTTCTGCTGCCAGACAATCACTATCTGAGCACGCAAAGCGTTCTGT
                     CTAAAGATCCGAACGAGAAACGCGATCATATGGTTCTGCTGGAGTTCGTAACCGCAGC
                     GGGCATCACGCATGGTATGGATGAACTGTACAAATGA"
                     /label="TGCGTAAAGGCGAAGAGCTGTTCACTGGTGTCGTCCCTATTCTGGTGGAA
                     CTGGATGGTGATGTCAACGGTCATAAGTTTTCCGTGCGTGGCGAGGGTGAAGGTGACG
                     CAACTAATGGTAAACTGACGCTGAAGTTCATCTGTACTACTGGTAAACTGCCGGTACC
                     TTGGCCGACTCTGGTAACGACGCTGACTTATGGTGTTCAGTGCTTTGCTCGTTATCCG
                     GACCATATGAAGCAGCATGACTTCTTCAAGTCCGCCATGCCGGAAGGCTATGTGCAGG
                     AACGCACGATTTCCTTTAAGGATGACGGCACGTACAAAACGCGTGCGGAAGTGAAATT
                     TGAAGGCGATACCCTGGTAAACCGCATTGAGCTGAAAGGCATTGACTTTAAAGAAGAC
                     GGCAATATCCTGGGCCATAAGCTGGAATACAATTTTAACAGCCACAATGTTTACATCA
                     CCGCCGATAAACAAAAAAATGGCATTAAAGCGAATTTTAAAATTCGCCACAACGTGGA
                     GGATGGCAGCGTGCAGCTGGCTGATCACTACCAGCAAAACACTCCAATCGGTGATGGT
                     CCTGTTCTGCTGCCAGACAATCACTATCTGAGCACGCAAAGCGTTCTGTCTAAAGATC
                     CGAACGAGAAACGCGATCATATGGTTCTGCTGGAGTTCGTAACCGCAGCGGGCATCAC
                     GCATGGTATGGATGAACTGTACAAATGA"
                     /note="Geneious type: Editing History Replacement"
     ligation        1..4
                     /label="Ligation"
     misc_feature    5..759
                     /Source="referenceToDocument: urn:local:.:32-824guws"
                     /label="Digestion of Amgen sfGFP - Fragment 2 extraction
                     (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     CDS             <5..762
                     /created_by="User"
                     /label="sfGFP BBa Safe"
     misc_feature    <5..>27
                     /Source="referenceToDocument: urn:local:.:33-824guws"
                     /label="deGFP (21) (concatenated sequence 3)"
                     /note="Geneious type: Concatenated sequence"
     misc_difference <5..27
                     /created_by="User"
                     /label="Linker"
     CDS             28..>741
                     /created_by="User"
                     /label="sfGFP BBa Safe"
     ligation        760..763
                     /label="Ligation"
     misc_feature    <760..>3290
                     /Original_Bases="TGTGTTAGAAGGTCGAGTCACCATTAGCCAGGTCTCAAAGC
                     GGGCCCAAGTTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAAACA
                     TCTTAATCATGC"
                     /label="TGTGTTAGAAGGTCGAGTCACCATTAGCCAGGTCTCAAAGCGGGCCCAAG
                     TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAAACATCTTAATCA
                     TGC"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    764..3286
                     /Source="referenceToDocument: urn:local:.:34-824guws"
                     /label="Digestion of Amgen pSG66 backbone - Fragment 2
                     extraction (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    <764..>3252
                     /label="Digestion of piece 1 ext by BsaI - Fragment 2
                     (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     source          <764..3252
                     /dnas_title="pBEST-OR2-OR1-Pr-UTR1-deGFP-T500"
                     /label="source"
     PCR_product     join(3164..3252,<764..829)
                     /pair="Pair 1"
                     /set="800bp_amp"
                     /current=1
                     /score="0.000"
                     /modified_by="User"
                     /label="868bp-linear fragment"
     PCR_product     join(2940..3252,<764..1056)
                     /pair="Pair 3"
                     /set="1200bp_amp"
                     /current=0
                     /score="0.264"
                     /modified_by="User"
                     /label="1319bp-linear fragment"
     PCR_product     join(2696..3252,<764..1283)
                     /pair="Pair 1"
                     /set="1600bp_amp"
                     /current=1
                     /score="1.000"
                     /modified_by="User"
                     /label="1768bp-linear fragment"
     misc_feature    complement(766..804)
                     /NEB_Tm=91
                     /created_by="User"
                     /label="ZS3034rb"
                     /note="Geneious type: primer_bind_reverse"
     terminator      770..799
                     /note="T500"
                     /label="T500 terminator"
     misc_feature    complement(782..799)
                     /NEB_Tm=64
                     /created_by="User"
                     /label="ZS3033ra"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(787..804)
                     /NEB_Tm=65
                     /created_by="User"
                     /label="ZS3034ra"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    800..815
                     /created_by="User"
                     /label="ZS30218r"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(807..829)
                     /dnas_title="GGTTGAAGGCTCTCAAGGGCATC"
                     /primer="GGTTGAAGGCTCTCAAGGGCATC"
                     /set="800bp_amp"
                     /pair="Pair 1"
                     /current=1
                     /modified_by="User"
                     /label="ZS2011r"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(807..824)
                     /NEB_Tm=62
                     /created_by="User"
                     /label="ZS3035r"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     814..833
                     /NEB_Tm=64
                     /created_by="User"
                     /modified_by="User"
                     /label="ZS2101f"
     misc_feature    complement(824..843)
                     /NEB_Tm=64
                     /created_by="User"
                     /label="ZS21111r"
                     /note="Geneious type: primer_bind_reverse"
     conflict        836..860
                     /created_by="User"
                     /label="area for IA_v1, v2"
     conflict        839..853
                     /created_by="User"
                     /label="area for IB_v1, v2"
     primer_bind     848..867
                     /created_by="primer3"
                     /%GC="70.0"
                     /Tm="59.97"
                     /Hairpin="4.0"
                     /Primer-Dimer="2.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Sequence="GGGCGCGGGGCATGACTATC"
                     /annotation_group="PrimerPlugin
                     Groupd15da267-d8a2-4186-95fc-cf70491eedb3_urn:local:Zachar
                     y:1339793905171.9"
                     /label="4th forward primer"
     misc_feature    complement(873..899)
                     /NEB_Tm=64
                     /created_by="User"
                     /label="ZS3036r"
                     /note="Geneious type: primer_bind_reverse"
     modified_base   888
                     /created_by="User"
                     /label="c->g"
     misc_difference <928..>949
                     /created_by="User"
                     /label
     primer_bind     1003..1022
                     /created_by="User"
                     /label="30424b"
     misc_feature    complement(1032..1049)
                     /NEB_Tm=60
                     /created_by="User"
                     /label="ZS3037r"
                     /note="Geneious type: primer_bind_reverse"
     PCR_primer      complement(1033..1056)
                     /dnas_title="CACATGTTCTTTCCTGCGTTATCC"
                     /primer="CACATGTTCTTTCCTGCGTTATCC"
                     /set="1200bp_amp"
                     /pair="Pair 3"
                     /current=0
                     /label="PCR primer"
     misc_feature    complement(1074..1093)
                     /created_by="User"
                     /label="30425b"
                     /note="Geneious type: primer_bind_reverse"
     rep_origin      complement(1093..1775)
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /label="ColE1 origin"
     PCR_primer      complement(1260..1283)
                     /dnas_title="AAGGGAGAAAGGCGGACAGGTATC"
                     /primer="AAGGGAGAAAGGCGGACAGGTATC"
                     /set="1600bp_amp"
                     /pair="Pair 1"
                     /current=1
                     /label="PCR primer"
     misc_feature    complement(1285..1299)
                     /NEB_tm=67
                     /created_by="User"
                     /label="ZS3038r"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     1499..1518
                     /created_by="User"
                     /label="30424c"
     misc_feature    complement(1578..1597)
                     /created_by="User"
                     /label="30425c"
                     /note="Geneious type: primer_bind_reverse"
     CDS             complement(1870..2730)
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /modified_by="User"
                     /label="AmpR"
     primer_bind     2000..2018
                     /created_by="User"
                     /label="30424d"
     modified_base   2011
                     /created_by="User"
                     /label="a->t"
     misc_feature    complement(2080..2099)
                     /created_by="User"
                     /label="30425d"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    2261
                     /created_by="User"
                     /label="A->G unique mut. not inherited from 21p"
     primer_bind     2464..2483
                     /created_by="primer3"
                     /%GC="65.0"
                     /Tm="59.97"
                     /Hairpin="5.0"
                     /Primer-Dimer="1.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Sequence="GAGTTGCTCTTGCCCGGCGT"
                     /annotation_group="PrimerPlugin
                     Group35e94697-015b-4ba9-a196-d1b75a060cac_urn:local:Zachar
                     y:1339793905171.9"
                     /label="3rd forward primer"
     primer_bind     2465..2484
                     /created_by="primer3"
                     /%GC="65.0"
                     /Tm="59.97"
                     /Hairpin="5.0"
                     /Primer-Dimer="2.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Sequence="AGTTGCTCTTGCCCGGCGTC"
                     /annotation_group="PrimerPlugin
                     Group2b969374-cce2-477b-82d4-77ad94a045e5_urn:local:Zachar
                     y:1339793905171.9"
                     /label="2nd forward primer"
     misc_feature    2487
                     /created_by="User"
                     /label="nonsilenced mutation Ile -> Val"
     primer_bind     2501..2520
                     /created_by="User"
                     /label="30424e"
     misc_feature    complement(2576..2595)
                     /created_by="User"
                     /label="30425e"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     2692..2709
                     /NEB_Tm=63
                     /created_by="User"
                     /label="ZS3038f"
     PCR_primer      2696..2718
                     /dnas_title="GGAATAAGGGCGACACGGAAATG"
                     /primer="GGAATAAGGGCGACACGGAAATG"
                     /set="1600bp_amp"
                     /pair="Pair 1"
                     /current=1
                     /label="PCR primer"
     modified_base   2930
                     /created_by="User"
                     /label="c->g"
     PCR_primer      2940..2958
                     /dnas_title="TCTGGCGAATCCTCTGACC"
                     /primer="TCTGGCGAATCCTCTGACC"
                     /set="1200bp_amp"
                     /pair="Pair 3"
                     /current=0
                     /label="PCR primer"
     primer_bind     2942..2958
                     /NEB_tm=63
                     /created_by="User"
                     /label="ZS3037f"
     primer_bind     3000..3018
                     /created_by="User"
                     /label="30424f"
     modified_base   3032
                     /created_by="User"
                     /label="a->t"
     primer_bind     3062..3081
                     /NEB_Tm=61
                     /created_by="User"
                     /modified_by="User"
                     /label="ZS21111f"
     modified_base   3070
                     /created_by="User"
                     /modified_by="User"
                     /label="a->t"
     misc_feature    complement(3082..3101)
                     /neb_tm=64
                     /designed_by_primer3
                     /created_by="User"
                     /label="ZS21110r"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3082..3101)
                     /created_by="User"
                     /label="30425f"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     3092..3109
                     /NEb_Tm=60
                     /created_by="User"
                     /label="ZS3036f"
     conflict        3136..3152
                     /created_by="User"
                     /label="area for IA_vb1, vb2"
     conflict        3138..3155
                     /created_by="User"
                     /label="area for IA_vA1, IA_vA2"
     primer_bind     3164..3184
                     /dnas_title="CATGTGTGTGCTGTTCCGCTG"
                     /primer="CATGTGTGTGCTGTTCCGCTG"
                     /set="800bp_amp"
                     /pair="Pair 1"
                     /current=1
                     /modified_by="User"
                     /label="ZS2011f"
     primer_bind     3167..3183
                     /NEB_Tm=61
                     /created_by="User"
                     /label="ZS3035f"
     misc_feature    complement(3174..3189)
                     /created_by="User"
                     /label="ZS30218f"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     3187..3204
                     /NEB_Tm_62
                     /created_by="User"
                     /label="ZS3034f"
     misc_feature    3192..3246
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /label="OR2-OR1-Pr"
     primer_bind     3192..3209
                     /created_by="User"
                     /NEB_tm=63
                     /modified_by="User"
                     /label="ZS3033f"
     promoter        3211..3216
                     /created_by="User"
                     /label="-35"
     promoter        3234..3239
                     /created_by="User"
                     /modified_by="User"
                     /label="-10"
     ANNOTATION      complement(3249..>3252)
                     /created_by="User"
                     /label="use me"
     ANNOTATION      complement(<3253..>3286)
                     /created_by="User"
                     /label="use me"
     misc_feature    <3253..3286
                     /label="Digestion of piece 1 ext by BsaI - Fragment 2
                     (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     source          3253..>3286
                     /dnas_title="pBEST-OR2-OR1-Pr-UTR1-deGFP-T500"
                     /label="source"
     misc_feature    3253..3285
                     /note="UTR1"
                     /label="UTR1"
     PCR_product     3253..>3286
                     /pair="Pair 1"
                     /set="800bp_amp"
                     /current=1
                     /score="0.000"
                     /modified_by="User"
                     /label="868bp-linear fragment"
     PCR_product     3253..>3286
                     /pair="Pair 3"
                     /set="1200bp_amp"
                     /current=0
                     /score="0.264"
                     /modified_by="User"
                     /label="1319bp-linear fragment"
     PCR_product     3253..>3286
                     /pair="Pair 1"
                     /set="1600bp_amp"
                     /current=1
                     /score="1.000"
                     /modified_by="User"
                     /label="1768bp-linear fragment"
     primer_bind     3253..3282
                     /NEB_Tm=60
                     /created_by="User"
                     /modified_by="User"
                     /label="ZS3022f"
     ligation        3287..3290
                     /label="Ligation"
     misc_feature    3287
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     CDS             3288..4526
                     /created_by="User"
                     /label="b2AR"
     misc_feature    3291..4553
                     /Source="referenceToDocument: urn:local:.:35-824guws"
                     /label="Digestion of GGA b2AR - Fragment 2 extraction
                     (concatenated sequence 3)"
                     /note="Geneious type: Concatenated sequence"
     misc_difference 4527..>4553
                     /created_by="User"
                     /label="Linker"
     misc_feature    4527..>4
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
ORIGIN      
        1 gcattgtact tccagtctgg tggtgctatg cgtaaaggag aagaactttt cactggtgtc
       61 gtccctattc tggtggaact ggatggtgat gtcaacggtc ataagttttc cgtgcgtggc
      121 gagggtgaag gtgacgcaac taatggtaaa ctgacgctga agttcatctg tactactggt
      181 aaactgccgg taccttggcc gactctggta acgacgctga cttatggtgt tcagtgcttt
      241 gctcgttatc cggaccatat gaagcagcat gacttcttca agtccgccat gccggaaggc
      301 tatgtgcagg aacgcacgat ttcctttaag gatgacggca cgtacaaaac gcgtgcggaa
      361 gtgaaatttg aaggcgatac cctggtaaac cgcattgagc tgaaaggcat tgactttaaa
      421 gaagatggca atatcctggg ccataagctg gaatacaatt ttaacagcca caatgtttac
      481 atcaccgccg ataaacaaaa aaatggcatt aaagcgaatt ttaaaattcg ccacaacgtg
      541 gaggatggca gcgtgcagct ggctgatcac taccagcaaa acactccaat cggtgatggt
      601 cctgttctgc tgccagacaa tcactatctg agcacgcaaa gcgttctgtc taaagatccg
      661 aacgagaaac gcgatcatat ggttctgctg gagttcgtaa ccgcagcggg catcacgcat
      721 ggtatggatg aactgtacaa acatcatcat catcatcatt gaactcgagc aaagcccgcc
      781 gaaaggcggg cttttctgtg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc
      841 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtgtt ctttatcatg
      901 caactcgtag gacaggtgcc ggcagctctc ttccgcttcc tcgctcactt actcgctgcg
      961 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1021 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1081 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1141 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1201 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1261 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1321 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1381 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1441 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1501 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     1561 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     1621 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1681 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     1741 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     1801 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     1861 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     1921 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     1981 atctggcccc agtgctgcaa tgataccgcg tgacccacgc tcaccggctc cagatttatc
     2041 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     2101 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     2161 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     2221 ggcttcattc agctccggtt cccaacgatc aaggcgagtt gcatgatccc ccatgttgtg
     2281 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     2341 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     2401 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     2461 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt
     2521 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     2581 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     2641 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     2701 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     2761 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     2821 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     2881 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtg ttcaagaatt
     2941 ctggcgaatc ctctgaccag ccagaaaacg acctttctgt ggtgaaaccg gatgctgcaa
     3001 ttcagagcgc cagcaagtgg gggacagcag atgacctgac cgccgcagag tggatgtttg
     3061 acatggtgat gactatcgca ccatcagcca gaaaaccgaa ttttgctggg tgggctaacg
     3121 atatccgcct gatgcgtgaa cgtgacggac gtaaccaccg cgacatgtgt gtgctgttcc
     3181 gctgggcatg ctgagctaac accgtgcgtg ttgacaattt tacctctggc ggtgataatg
     3241 gttgcagcta gcaataattt tgtttaactt taagaaggag atatacaatg ggccagcctg
     3301 ggaacggatc agccttcctt ctggcaccca acgggtcaca cgctccagat catgacgtta
     3361 cccaagagcg tgacgaggtt tgggttgtag ggatgggcat cgttatgagc ctgatcgtat
     3421 tggccattgt attcggaaat gtgctggtga ttacagccat tgccaaattc gagcgtttac
     3481 aaactgtaac taattacttc atcactagtt tagcctgtgc tgaccttgtc atggggttag
     3541 cggttgttcc tttcggggct gctcatattt tgatgaagat gtggaccttt ggaaattttt
     3601 ggtgcgaatt ctggacaagc atcgatgttt tatgcgtaac cgcctcaatt gaaacacttt
     3661 gcgtaattgc ggtagatcgt tacttcgcca ttacatcgcc cttcaagtac caatctttgc
     3721 ttacgaagaa caaggcccgc gttatcatcc ttatggtctg gatcgtaagc ggtcttacgt
     3781 cgtttttgcc catccagatg cactggtatc gtgcaacaca tcaggaggcc attaactgtt
     3841 acgccaatga gacttgctgc gatttcttca caaaccaagc ctatgccatc gcaagctcaa
     3901 ttgtctcttt ttatgtgccg ttagtcatta tggtgtttgt gtactcccgt gtatttcaag
     3961 aagctaagcg tcagctgcaa aaaatcgaca aatctgaggg acgttttcac gttcaaaacc
     4021 tgtcccaagt cgaacaagat ggacgtactg gacacggctt gcgccgcagt tctaaatttt
     4081 gtcttaagga gcataaggcg ttgaagacgt taggtattat tatgggtacg tttacattgt
     4141 gttggcttcc gttttttatc gttaacatcg tccacgttat ccaagataac ttgatccgca
     4201 aggaggtgta tattttactt aactggattg gatatgtgaa ttcgggattc aaccccctta
     4261 tttactgccg ctctccagac ttccgcatcg cgttccaaga attgctgtgt ttgcgccgtt
     4321 caagtcttaa agcttatgga aatggttatt catctaatgg aaacacaggt gaacagtcag
     4381 gatatcacgt tgagcaggag aaggagaaca agttattatg cgaagacctt ccgggcacgg
     4441 aagactttgt aggacatcaa ggcaccgtac ccagtgataa tattgattct caaggtcgta
     4501 attgcagcac caatgattcg cttctgggtg aaaacctgta cttccagtct ggt
//
