LOCUS       pSG05:_pGlnA-UTR1-deGFP-T500        3541 bp ds-DNA     circular     14-AUG-2017
DEFINITION  PglnA-deGFP (based on p01).
ACCESSION   urn.local.shaobinguo.d-829pf9g
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     from pBEST-p15A-OR2-OR1-P1-UTR1-deGFP-T500, insertion of PL-tetO1
            promoter/operator between SphI-NheI.
            TCCCTATCAGTGATAGAGATTGACATCCCTATCAGTGATAGAGATACTGAGCACA
            ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     promoter        1..308
                     /created_by="User"
                     /label="glnA"
     5'UTR           309..342
                     /created_by="User"
                     /label="UTR"
     misc_feature    complement(312..348)
                     /created_by="User"
                     /modified_by="User"
                     /label="Oligo_06r"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    343..348
                     /created_by="User"
                     /label="NcoI"
                     /note="Geneious type: restriction enzyme site"
     gene            345..1022
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /modified_by="User"
                     /label="deGFP"
     misc_feature    1029..1058
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /label="T500"
     100bp           1059..1158
                     /created_by="User"
     primer          1133..1158
                     /created_by="User"
                     /label="reverse oligo_01(antiparallel)"
     misc_feature    complement(1209..2117)
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /label="p15A ori"
     CDS             complement(2220..3080)
                     /created_by="User"
                     /label="AmpR"
     misc_feature    complement(2340..2359)
                     /created_by="primer3"
                     /annotation_group="1361577095979161000cfd64310-5db6-422c-a
                     9fe-4706ae730b5c"
                     /%GC="55.0"
                     /Tm="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="6.4"
                     /Pair_Dimer_Tm="24.7"
                     /Product_Size=2312
                     /Sequence="GCGGTATCATTGCAGCACTG"
                     /label="1st reverse primer"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(2340..2359)
                     /created_by="primer3"
                     /annotation_group="1361577095979628000014c052c-e4bc-4ea4-b
                     230-70601d328db9"
                     /%GC="55.0"
                     /Tm="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="6.4"
                     /Pair_Dimer_Tm="24.9"
                     /Product_Size=2313
                     /Sequence="GCGGTATCATTGCAGCACTG"
                     /label="2nd reverse primer"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     3340..3359
                     /created_by="primer3"
                     /annotation_group="1361577095979628000014c052c-e4bc-4ea4-b
                     230-70601d328db9"
                     /%GC="55.0"
                     /Tm="60.0"
                     /Hairpin_Tm="45.7"
                     /Self_Dimer_Tm="0.7"
                     /Pair_Dimer_Tm="24.9"
                     /Product_Size=2313
                     /Sequence="GGATGCTGCAATTCAGAGCG"
                     /label="2nd forward primer"
     primer_bind     3341..3360
                     /created_by="primer3"
                     /annotation_group="1361577095979161000cfd64310-5db6-422c-a
                     9fe-4706ae730b5c"
                     /%GC="55.0"
                     /Tm="60.0"
                     /Hairpin_Tm="45.7"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="24.7"
                     /Product_Size=2312
                     /Sequence="GATGCTGCAATTCAGAGCGG"
                     /label="1st forward primer"
     100bp           complement(3442..3541)
                     /created_by="User"
                     /modified_by="User"
                     /label="100bp protection"
     primer          3442..3462
                     /created_by="User"
                     /label="forward oligo_01"
     primer          3522..3541
                     /created_by="User"
                     /modified_by="User"
                     /label="pBESTtetO (sequencing primer)"
     misc_feature    3536..3541
                     /created_by="User"
                     /label="SphI"
                     /note="Geneious type: restriction enzyme site"
     primer_bind     3536..38
                     /created_by="User"
                     /label="oligo_06f"
ORIGIN      
        1 atcatcttat taatcagata aaatatttct agagcatcct ccgcaaacaa gtattgcaga
       61 gtccctttgt gatcgctttc acggagcata aaaagggtta tccaaaggtc attgcaccaa
      121 catggtgctt aatgtttcca ttgaagcact atattggtgc aacattcaca tcgtggtgca
      181 gcccttttgc acgatggtgc gcatgataac gccttttagg ggcaatttaa aagttggcac
      241 agatttcgct ttatcttttt tacggcgaca cggccaaaat aattgcagat ttcgttacca
      301 cgacgaccta actttcaatt ctaataagga ggaagacttc aaccatggag cttttcactg
      361 gcgttgttcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      421 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      481 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
      541 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      601 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      661 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      721 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
      781 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
      841 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
      901 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
      961 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatct
     1021 aactcgagca aagcccgccg aaaggcgggc ttttctgtgt cgaccgatgc ccttgagagc
     1081 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
     1141 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcatgataag ctgtcaaaca
     1201 tgagaattac aacttatatc gtatggggct gacttcaggt gctacatttg aagagataaa
     1261 ttgcactgaa atctagaaat attttatctg attaataaga tgatcttctt gagatcgttt
     1321 tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg gcggtttttc
     1381 gaaggttctc tgagctacca actctttgaa ccgaggtaac tggcttggag gagcgcagtc
     1441 accaaaactt gtcctttcag tttagcctta accggcgcat gacttcaaga ctaactcctc
     1501 taaatcaatt accagtggct gctgccagtg gtgcttttgc atgtctttcc gggttggact
     1561 caagacgata gttaccggat aaggcgcagc ggtcggactg aacggggggt tcgtgcatac
     1621 agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga atgagacaaa
     1681 cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg
     1741 agggagccgc caggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact
     1801 gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggct
     1861 ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca ggaaatctcc
     1921 gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt
     1981 gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg gtgcagcctt
     2041 ttttctcctg ccacatgaag cacttcactg acaccctcat cagtgccaac atagtaagcc
     2101 agtatacact ccgctagggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     2161 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     2221 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     2281 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     2341 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     2401 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     2461 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     2521 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     2581 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     2641 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     2701 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     2761 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     2821 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     2881 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     2941 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     3001 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     3061 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     3121 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     3181 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     3241 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt ctggcgaatc
     3301 ctctgaccag ccagaaaacg acctttctgt ggtgaaaccg gatgctgcaa ttcagagcgg
     3361 cagcaagtgg gggacagcag aagacctgac cgccgcagag tggatgtttg acatggtgaa
     3421 gactatcgca ccatcagcca gaaaaccgaa ttttgctggg tgggctaacg atatccgcct
     3481 gatgcgtgaa cgtgacggac gtaaccaccg cgacatgtgt gtgctgttcc gctgggcatg
     3541 c
//
