LOCUS       Exported                4017 bp ds-DNA     circular SYN 18-SEP-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    HHM iATPSnFR1.1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 22, 2017 from SnapGene Viewer 3.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        20..38
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             70..92
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             103..120
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     CDS             124..150
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /translation="YPYDVPDYA"
     misc_feature    172..1308
                     /note="iATPSnFR1.1"
     CDS             499..1227
                     /codon_start=1
                     /note="cpSFGFP"
                     /translation="SHNVYITADKQKNGIKANFKIRHNVEDGSMQLADHYQQNTPIGDG
                     PVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMSKGEE
                     LFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNF"
     CDS             1321..1350
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /note="Myc"
                     /translation="EQKLISEEDL"
     terminator      1433..1480
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1577..2031
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2057..2161
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             2162..3022
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3193..3781
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
       61 agaaataatt ttgtttaact ttaagaagga gatatacata tgcatcatca tcatcatcat
      121 ggttatccct atgatgttcc agattatgct ggggcccagc cggccagatc tatgaagact
      181 attcacgtga gtgtcgtaac tcccgacggg cctgtatatg aggatgacgt tgaaatggtg
      241 agcgtcaaag caaaaagtgg cgagctcggt attctcccag gccacattcc cttgaaagct
      301 cccctagaga tcagtgccgc acgcctgaag aaagggggca aaacacagta tatcgctgtg
      361 tcaggcggca acctcgaagt gcggcctgac aaggtgacca tctatgccca ggcagccgag
      421 agggctgagg atatcgatgt cctgcgcgcc aagaaagcca aggagagagc cgagcgccga
      481 ctccaatcac aggtcctgag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc
      541 atcaaggcga acttcaagat ccgccacaac gtggaggacg gcagcatgca gctcgccgac
      601 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
      661 ctgagcaccc agtccgtgct gagcaaagac cctaacgaga agcgcgatca catggtcctg
      721 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggcggt
      781 accggaggga gcatgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      841 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgc gcggcgaggg cgagggcgat
      901 gccaccaacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      961 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     1021 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     1081 accatcagct tcaaggacga cggcacctac aagacccgcg ccgaggtgaa gttcgagggc
     1141 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1201 ctggggcaca agctggagta caactttggg ttgcacgaca tcgattttaa gcgcgccgag
     1261 ctcagcctta agcgcgcaat gaataggctc tcagttgccg aaatgaagct gcaggtcgac
     1321 gaacaaaaac tcatctcaga agaggatctg aattaagaat tcgaagcttg atccggctgc
     1381 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
     1441 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
     1501 cggatctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     1561 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     1621 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     1681 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     1741 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     1801 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     1861 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     1921 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     1981 atttaacaaa aatttaacgc gaattttaac aaaatattac gcttacaatt taggtggcac
     2041 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     2101 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     2161 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     2221 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     2281 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     2341 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     2401 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     2461 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     2521 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     2581 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     2641 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     2701 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     2761 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     2821 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     2881 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     2941 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     3001 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     3061 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     3121 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     3181 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3241 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3301 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     3361 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3421 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3481 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3541 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3601 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3661 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3721 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3781 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3841 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     3901 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     3961 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcag
//
