LOCUS       Exported                6423 bp ds-DNA     circular SYN 08-SEP-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pDisplayMini iATPSnFR1.0
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 22, 2017 from SnapGene Viewer 3.1.4
            http://www.snapgene.com
COMMENT     LOCUS 8N5C_pDisplay2_Ann 7558 bp ds-DNA circular 29-AUG-2008 
            ACCESSION VERSION SOURCE ORGANISM COMMENT COMMENT 
            ApEinfo:methylated:0
FEATURES             Location/Qualifiers
     source          1..6423
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..6423
                     /note="Untitled"
                     /note="                                                    
                                "
     primer_bind     637..656
                     /note="T7"
     misc_feature    736..798
                     /note="Ig K-Chain"
     misc_feature    799..804
                     /note="MCS"
     misc_feature    805..1125
                     /note="PS3 epsilon"
     misc_feature    829..831
                     /note="T9V"
     misc_feature    1036..1038
                     /note="N78Y"
     misc_feature    1126..1131
                     /note="L1"
     misc_feature    1132..1407
                     /note="cpsfGFP"
     misc_feature    1219..1221
                     /note="V->M"
     misc_feature    1408..1425
                     /note="cpsfGFP"
     misc_feature    1426..1860
                     /note="cpSFGFP"
     misc_feature    1870..1941
                     /note="PS3 epsilon"
     misc_feature    1972..2001
                     /note="myc_epi"
     misc_feature    2005..2154
                     /note="PDGFR 513-561"
     misc_feature    2173..2190
                     /note="BGH_rev"
     rep_origin      complement(2517..3199)
                     /direction=LEFT
                     /note="ColE1 origin"
     misc_feature    2976
                     /note="G->A"
     misc_feature    4048..4053
                     /note="weird insertion"
     CDS             complement(4976..5635)
                     /note="AmpR"
     rep_origin      complement(5965..6421)
                     /direction=LEFT
                     /note="F1 ori"
ORIGIN
        1 cgcgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
       61 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      121 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      181 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      241 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      301 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      361 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      421 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      481 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      541 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagaa ctctctggct
      601 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      661 ccaagcttgg taccgaactc ggatccacta gtaacggccg ccagtgtgct ggaattcggc
      721 ttggggatat ccaccatgga gacagacaca ctcctgctat gggtactgct gctctgggtt
      781 ccaggttcca ctggtgacag atctatgaag actattcacg tgagtgtcgt aactcccgac
      841 gggcctgtat atgaggatga cgttgaaatg gtgagcgtca aagcaaaaag tggcgagctc
      901 ggtattctcc caggccacat tcccttgaaa gctcccctag agatcagtgc cgcacgcctg
      961 aagaaagggg gcaaaacaca gtatatcgct gtgtcaggcg gcaacctcga agtgcggcct
     1021 gacaaggtga ccatctatgc ccaggcagcc gagagggctg aggatatcga tgtcctgcgc
     1081 gccaaggccg ccaaggagag agccgagcgc cgactccaat cacaggtcct gagccacaac
     1141 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg cgaacttcaa gatccgccac
     1201 aacgtggagg acggcagcat gcagctcgcc gaccactacc agcagaacac ccccatcggc
     1261 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgt gctgagcaaa
     1321 gaccctaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1381 actctcggca tggacgagct gtacaagggc ggtaccggag ggagcatgag caagggcgag
     1441 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     1501 aagttcagcg tgcgcggcga gggcgagggc gatgccacca acggcaagct gaccctgaag
     1561 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     1621 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     1681 tccgccatgc ccgaaggcta cgtccaggag cgcaccatca gcttcaagga cgacggcacc
     1741 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1801 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacttt
     1861 gggttgcacg acatcgattt taagcgcgcc gagctcgccc ttaagcgcgc aatgaatagg
     1921 ctctcagttg ccgaaatgaa gggagggacc ggcggtagcc tgcaggtcga cgaacaaaaa
     1981 ctcatctcag aagaggatct gaatgctgtg ggccaggaca cgcaggaggt catcgtggtg
     2041 ccacactcct tgccctttaa ggtggtggtg atctcagcca tcctggccct ggtggtgctc
     2101 accatcatct cccttatcat cctcatcatg ctttggcaga agaagccacg ttaggcggcc
     2161 gctcgagatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     2221 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     2281 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     2341 acagcaaggg ggaggattgg gaagacaata gcaggcgtgc tggggatgcg gtgggctcta
     2401 tggcttctga ggcggaaaga accagtggcg gtaatacggt tatccacaga atcaggggat
     2461 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2521 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2581 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2641 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2701 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     2761 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     2821 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2881 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     2941 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     3001 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3061 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3121 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     3181 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     3241 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaac ctgaggctat ggcagggcct
     3301 gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg gtggggtggg
     3361 gaaaaggaag aaacgcgggc gtattggccc caatggggtc tcggtggggt atcgacagag
     3421 tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc gtgcgtttta
     3481 ttctgtcttt ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt
     3541 tcagttagcc tccccctagg gtgggcgaag aactccagca tgagatcccc gcgctggagg
     3601 atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata gaaggcggcg
     3661 gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc
     3721 agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg
     3781 gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag
     3841 caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac
     3901 agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc
     3961 catgggtcac gacgagatcc tcgccgtcgg gcttgctcgc cttgagcctg gcgaacagtt
     4021 cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt
     4081 ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag
     4141 ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag
     4201 gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc
     4261 ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc
     4321 acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg tcggtcttga
     4381 caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga
     4441 ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg
     4501 cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcgatct
     4561 ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc
     4621 gggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg
     4681 aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact
     4741 aattgagatg cttgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc
     4801 tggttgctga ctaattgaga tgcttgcttt gcatacttct gcctgctggg gagcctgggg
     4861 actttccaca ccctaactga cacacattcc acagctggtt ctttccgcct caggactctt
     4921 cctttttcaa taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4981 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     5041 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     5101 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     5161 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5221 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5281 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5341 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5401 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5461 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5521 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5581 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5641 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5701 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5761 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5821 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5881 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5941 catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6001 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6061 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6121 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     6181 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     6241 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     6301 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     6361 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     6421 acg
//
