LOCUS       Exported                5785 bp ds-DNA     circular SYN 18-SEP-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    synapsin iATP anotated
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 22, 2017 from SnapGene Viewer 3.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        266..713
                     /note="hSyn promoter"
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             741..803
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /note="Ig-kappa leader"
                     /translation="METDTLLLWVLLLWVPGSTGD"
     CDS             810..1130
                     /codon_start=1
                     /note="PS3 epsilon 1-106"
                     /translation="MKTIHVSVVTPDGPVYEDDVEMVSVKAKSGELGILPGHIPLKAPL
                     EISAARLKKGGKTQYIAVSGGNLEVRPDKVTIYAQAAERAEDIDVLRAKAAKERAERRL
                     QSQ"
     CDS             1137..1865
                     /codon_start=1
                     /note="cpSFGFP"
                     /translation="SHNVYITADKQKNGIKANFKIRHNVEDGSMQLADHYQQNTPIGDG
                     PVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMSKGEE
                     LFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNF"
     CDS             1875..1946
                     /codon_start=1
                     /note="PS3 epsilon 111-133"
                     /translation="DIDFKRAELALKRAMNRLSVAEMK"
     CDS             1977..2006
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /note="Myc"
                     /translation="EQKLISEEDL"
     CDS             2010..2156
                     /codon_start=1
                     /note="PDGFR 513-561"
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     misc_feature    2172..2760
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(2643..2654)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     primer_bind     complement(2763..2779)
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2809..2930
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3240..3256)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3398..3853
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3879..3983
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             3984..4844
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5015..5603
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgcgc
       61 gctcgctcgc tcactgaggc cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc
      121 ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat cactaggggt
      181 tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg ctctaggaag
      241 atctctgcag agggccctgc gtatgagtgc aagtgggttt taggaccagg atgaggcggg
      301 gtgggggtgc ctacctgacg accgaccccg acccactgga caagcaccca acccccattc
      361 cccaaattgc gcatccccta tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt
      421 gcgcactgcc agcttcagca ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc
      481 accgccgcct cagcactgaa ggcgcgctga cgtcactcgc cggtcccccg caaactcccc
      541 ttcccggcca ccttggtcgc gtccgcgccg ccgccggccc agccggaccg caccacgcga
      601 ggcgcgagat aggggggcac gggcgcgacc atctgcgctg cggcgccggc gactcagcgc
      661 tgcctcagtc tgcggtgggc agcggaggag tcgtgtcgtg cctgagagcg cagtcgaatt
      721 caagctgcta gcaaggatcc atggagacag acacactcct gctatgggta ctgctgctct
      781 gggttccagg ttccactggt gacagatcta tgaagactat tcacgtgagt gtcgtaactc
      841 ccgacgggcc tgtatatgag gatgacgttg aaatggtgag cgtcaaagca aaaagtggcg
      901 agctcggtat tctcccaggc cacattccct tgaaagctcc cctagagatc agtgccgcac
      961 gcctgaagaa agggggcaaa acacagtata tcgctgtgtc aggcggcaac ctcgaagtgc
     1021 ggcctgacaa ggtgaccatc tatgcccagg cagccgagag ggctgaggat atcgatgtcc
     1081 tgcgcgccaa ggccgccaag gagagagccg agcgccgact ccaatcacag gtcctgagcc
     1141 acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggcgaac ttcaagatcc
     1201 gccacaacgt ggaggacggc agcatgcagc tcgccgacca ctaccagcag aacaccccca
     1261 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgtgctga
     1321 gcaaagaccc taacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     1381 ggatcactct cggcatggac gagctgtaca agggcggtac cggagggagc atgagcaagg
     1441 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     1501 gccacaagtt cagcgtgcgc ggcgagggcg agggcgatgc caccaacggc aagctgaccc
     1561 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     1621 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     1681 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcagcttc aaggacgacg
     1741 gcacctacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     1801 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     1861 actttgggtt gcacgacatc gattttaagc gcgccgagct cgcccttaag cgcgcaatga
     1921 ataggctctc agttgccgaa atgaagggag ggaccggcgg tagcctgcag gtcgacgaac
     1981 aaaaactcat ctcagaagag gatctgaatg ctgtgggcca ggacacgcag gaggtcatcg
     2041 tggtgccaca ctccttgccc tttaaggtgg tggtgatctc agccatcctg gccctggtgg
     2101 tgctcaccat catctccctt atcatcctca tcatgctttg gcagaagaag ccacgttaga
     2161 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     2221 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     2281 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     2341 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     2401 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     2461 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     2521 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     2581 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     2641 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     2701 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     2761 atcgataccg tcgacctcga cccgggcggc cgcttcgagc agacatgata agatacattg
     2821 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     2881 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     2941 attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt taaagcaagt
     3001 aaaacctcta caaatgtggt aaaatcgata aggatcttcc tagagcatgg ctacgtagat
     3061 aagtagcatg gcgggttaat cattaactac aaggaacccc tagtgatgga gttggccact
     3121 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
     3181 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gccttaatta acctaattca
     3241 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     3301 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     3361 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     3421 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     3481 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     3541 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     3601 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     3661 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     3721 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     3781 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     3841 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     3901 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     3961 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     4021 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     4081 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     4141 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     4201 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     4261 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     4321 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     4381 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     4441 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     4501 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     4561 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     4621 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     4681 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     4741 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     4801 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     4861 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     4921 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     4981 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5041 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5101 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5161 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5221 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5281 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5341 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5401 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5461 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5521 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5581 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5641 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     5701 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     5761 agtcagtgag cgaggaagcg gaaga
//
