LOCUS Exported 5506 bp ds-DNA circular SYN 18-SEP-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS syn-cytoplasmic iATPSnFR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5506) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Sep 22, 2017 from SnapGene Viewer 3.1.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5506 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 266..713 /note="hSyn promoter" /note="human synapsin I promoter; confers neuron-specific expression (Kugler et al., 2003)" CDS 741..1061 /codon_start=1 /note="PS3 1-106" /translation="MKTIHVSVVTPDGPVYEDDVEMVSVKAKSGELGILPGHIPLKAPL EISAARLKKGGKTQYIAVSGGNLEVRPDKVTIYAQAAERAEDIDVLRAKAAKERAERRL QSQ" CDS 1068..1796 /codon_start=1 /note="cpSFGFP" /translation="SHNVYITADKQKNGIKANFKIRHNVEDGSMQLADHYQQNTPIGDG PVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMSKGEE LFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLT YGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIE LKGIDFKEDGNILGHKLEYNF" misc_feature 1806..1876 /note="PS3 epsilon 111-133" misc_feature 1893..2481 /note="WPRE" /note="woodchuck hepatitis virus posttranscriptional regulatory element" CDS complement(2364..2375) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /translation="IEGR" primer_bind complement(2484..2500) /note="KS primer" /note="common sequencing primer, one of multiple similar variants" polyA_signal 2530..2651 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" primer_bind complement(2961..2977) /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" rep_origin 3119..3574 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3600..3704 /gene="bla" /note="AmpR promoter" CDS 3705..4565 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 4736..5324 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgcgc 61 gctcgctcgc tcactgaggc cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc 121 ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat cactaggggt 181 tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg ctctaggaag 241 atctctgcag agggccctgc gtatgagtgc aagtgggttt taggaccagg atgaggcggg 301 gtgggggtgc ctacctgacg accgaccccg acccactgga caagcaccca acccccattc 361 cccaaattgc gcatccccta tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt 421 gcgcactgcc agcttcagca ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc 481 accgccgcct cagcactgaa ggcgcgctga cgtcactcgc cggtcccccg caaactcccc 541 ttcccggcca ccttggtcgc gtccgcgccg ccgccggccc agccggaccg caccacgcga 601 ggcgcgagat aggggggcac gggcgcgacc atctgcgctg cggcgccggc gactcagcgc 661 tgcctcagtc tgcggtgggc agcggaggag tcgtgtcgtg cctgagagcg cagtcgaatt 721 caagctgcta gcaaggatcc atgaagacta ttcacgtgag tgtcgtaact cccgacgggc 781 ctgtatatga ggatgacgtt gaaatggtga gcgtcaaagc aaaaagtggc gagctcggta 841 ttctcccagg ccacattccc ttgaaagctc ccctagagat cagtgccgca cgcctgaaga 901 aagggggcaa aacacagtat atcgctgtgt caggcggcaa cctcgaagtg cggcctgaca 961 aggtgaccat ctatgcccag gcagccgaga gggctgagga tatcgatgtc ctgcgcgcca 1021 aggccgccaa ggagagagcc gagcgccgac tccaatcaca ggtcctgagc cacaacgtct 1081 atatcaccgc cgacaagcag aagaacggca tcaaggcgaa cttcaagatc cgccacaacg 1141 tggaggacgg cagcatgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 1201 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgtgctg agcaaagacc 1261 ctaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 1321 tcggcatgga cgagctgtac aagggcggta ccggagggag catgagcaag ggcgaggagc 1381 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt 1441 tcagcgtgcg cggcgagggc gagggcgatg ccaccaacgg caagctgacc ctgaagttca 1501 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg 1561 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg 1621 ccatgcccga aggctacgtc caggagcgca ccatcagctt caaggacgac ggcacctaca 1681 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg 1741 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactttgggt 1801 tgcacgacat cgattttaag cgcgccgagc tcgcccttaa gcgcgcaatg aataggctct 1861 cagttgccga aatgaagtag aagcttatcg ataatcaacc tctggattac aaaatttgtg 1921 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 1981 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 2041 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 2101 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 2161 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 2221 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 2281 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg 2341 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 2401 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 2461 ccctttgggc cgcctccccg catcgatacc gtcgacctcg acccgggcgg ccgcttcgag 2521 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa 2581 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca 2641 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt 2701 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatcttc 2761 ctagagcatg gctacgtaga taagtagcat ggcgggttaa tcattaacta caaggaaccc 2821 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga 2881 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc 2941 agccttaatt aacctaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct 3001 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc 3061 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac 3121 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 3181 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 3241 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 3301 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 3361 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 3421 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 3481 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 3541 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc 3601 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac 3661 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt 3721 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag 3781 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg 3841 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa 3901 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc 3961 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag 4021 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa 4081 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc 4141 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg 4201 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa 4261 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa 4321 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg 4381 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag 4441 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg 4501 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt 4561 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt 4621 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 4681 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 4741 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 4801 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 4861 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga 4921 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 4981 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 5041 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 5101 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 5161 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 5221 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 5281 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 5341 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 5401 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca 5461 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaaga //