LOCUS       Exported                5506 bp ds-DNA     circular SYN 18-SEP-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    syn-cytoplasmic iATPSnFR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 22, 2017 from SnapGene Viewer 3.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        266..713
                     /note="hSyn promoter"
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             741..1061
                     /codon_start=1
                     /note="PS3 1-106"
                     /translation="MKTIHVSVVTPDGPVYEDDVEMVSVKAKSGELGILPGHIPLKAPL
                     EISAARLKKGGKTQYIAVSGGNLEVRPDKVTIYAQAAERAEDIDVLRAKAAKERAERRL
                     QSQ"
     CDS             1068..1796
                     /codon_start=1
                     /note="cpSFGFP"
                     /translation="SHNVYITADKQKNGIKANFKIRHNVEDGSMQLADHYQQNTPIGDG
                     PVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMSKGEE
                     LFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNF"
     misc_feature    1806..1876
                     /note="PS3 epsilon 111-133"
     misc_feature    1893..2481
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(2364..2375)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     primer_bind     complement(2484..2500)
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2530..2651
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2961..2977)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3119..3574
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3600..3704
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             3705..4565
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4736..5324
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgcgc
       61 gctcgctcgc tcactgaggc cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc
      121 ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat cactaggggt
      181 tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg ctctaggaag
      241 atctctgcag agggccctgc gtatgagtgc aagtgggttt taggaccagg atgaggcggg
      301 gtgggggtgc ctacctgacg accgaccccg acccactgga caagcaccca acccccattc
      361 cccaaattgc gcatccccta tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt
      421 gcgcactgcc agcttcagca ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc
      481 accgccgcct cagcactgaa ggcgcgctga cgtcactcgc cggtcccccg caaactcccc
      541 ttcccggcca ccttggtcgc gtccgcgccg ccgccggccc agccggaccg caccacgcga
      601 ggcgcgagat aggggggcac gggcgcgacc atctgcgctg cggcgccggc gactcagcgc
      661 tgcctcagtc tgcggtgggc agcggaggag tcgtgtcgtg cctgagagcg cagtcgaatt
      721 caagctgcta gcaaggatcc atgaagacta ttcacgtgag tgtcgtaact cccgacgggc
      781 ctgtatatga ggatgacgtt gaaatggtga gcgtcaaagc aaaaagtggc gagctcggta
      841 ttctcccagg ccacattccc ttgaaagctc ccctagagat cagtgccgca cgcctgaaga
      901 aagggggcaa aacacagtat atcgctgtgt caggcggcaa cctcgaagtg cggcctgaca
      961 aggtgaccat ctatgcccag gcagccgaga gggctgagga tatcgatgtc ctgcgcgcca
     1021 aggccgccaa ggagagagcc gagcgccgac tccaatcaca ggtcctgagc cacaacgtct
     1081 atatcaccgc cgacaagcag aagaacggca tcaaggcgaa cttcaagatc cgccacaacg
     1141 tggaggacgg cagcatgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     1201 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgtgctg agcaaagacc
     1261 ctaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     1321 tcggcatgga cgagctgtac aagggcggta ccggagggag catgagcaag ggcgaggagc
     1381 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     1441 tcagcgtgcg cggcgagggc gagggcgatg ccaccaacgg caagctgacc ctgaagttca
     1501 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     1561 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     1621 ccatgcccga aggctacgtc caggagcgca ccatcagctt caaggacgac ggcacctaca
     1681 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     1741 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactttgggt
     1801 tgcacgacat cgattttaag cgcgccgagc tcgcccttaa gcgcgcaatg aataggctct
     1861 cagttgccga aatgaagtag aagcttatcg ataatcaacc tctggattac aaaatttgtg
     1921 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     1981 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     2041 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     2101 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     2161 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     2221 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     2281 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     2341 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     2401 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     2461 ccctttgggc cgcctccccg catcgatacc gtcgacctcg acccgggcgg ccgcttcgag
     2521 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     2581 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     2641 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt
     2701 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatcttc
     2761 ctagagcatg gctacgtaga taagtagcat ggcgggttaa tcattaacta caaggaaccc
     2821 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     2881 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     2941 agccttaatt aacctaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     3001 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     3061 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     3121 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3181 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3241 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     3301 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     3361 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     3421 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     3481 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     3541 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     3601 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3661 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3721 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     3781 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     3841 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     3901 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     3961 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     4021 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     4081 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     4141 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4201 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     4261 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4321 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4381 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     4441 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4501 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     4561 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     4621 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4681 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4741 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4801 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4861 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     4921 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4981 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5041 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5101 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     5161 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5221 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5281 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5341 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     5401 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     5461 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaaga
//
