LOCUS pCH1/RAD51Opt 6645 bp DNA circular 19-MAR-1980 DEFINITION pCOLADuet-1/GroE/RAD51-Opt. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT LSOWNER| COMMENT VNTNAME|pCH1-RAD51Opt| COMMENT VNTAUTHORNAME|Shyamal Subramanyam| COMMENT VNTREPLTYPE|Plasmid COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pCH1-RAD51Opt" 0 6645 COMMENT (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) COMMENT (CObList) -1 "") COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList "BglII" "HindIII" "NcoI" "NdeI" "NotI" "SmaI" "XmaI") COMMENT (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 COMMENT "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 4294967295 1 6645 0 0 0 0 0 0 0 0) (CStringList) COMMENT (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList #0=(CRSite (CStringList) "BglII" "agatct" 2 0 1 3227 0 0 "") COMMENT #1=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") COMMENT #2=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 70 0 0 "") COMMENT #3=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 1241 0 0 "") COMMENT #4=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 1093 0 0 "") COMMENT #5=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 4175 0 0 "") COMMENT #6=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 4173 0 0 "")) COMMENT (CObList COMMENT #7=(CFSignal (CObList) "ColA Ori" 33 0 0 4590 5225 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "4590..5225") COMMENT #8=(CFSignal (CObList) "LacI" 4 0 1 5423 6505 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(5423..6505)") COMMENT #9=(CFSignal (CObList) "T7 Promoter" 30 0 0 1156 1172 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1156..1172") COMMENT #10=(CFSignal (CObList) "T7 Promoter" 30 0 0 6629 6645 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "6629..6645") COMMENT #11=(CFSignal (CObList) "KanR" 4 0 1 3660 4480 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(3660..4480)") COMMENT #12=(CFSignal (CObList) "GroE Operon" 103 0 0 1241 3226 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1241..3226") COMMENT #13=(CFSignal (CObList) "LacO" 31 0 0 5 25 0 (CStringList) (CStringList) COMMENT 1 1 1 1 "5..25") COMMENT #14=(CFSignal (CObList) "LacO" 31 0 0 1177 1197 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1177..1197") COMMENT #15=(CFSignal (CObList) "RBS-2" 32 0 0 1228 1233 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1228..1233") COMMENT #16=(CFSignal (CObList) "RBS-1" 32 0 0 58 63 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "58..63") COMMENT #17=(CFSignal (CObList) "RBS-3" 21 0 0 1564 1569 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1564..1569") COMMENT #18=(CFSignal (CObList) "GroES" 4 0 0 1242 1535 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1242..1535") COMMENT #19=(CFSignal (CObList) "GroEL" 4 0 0 1579 3225 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1579..3225") COMMENT #20=(CFSignal (CObList) "S.Tag" 31 0 0 3287 3331 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "3287..3331") COMMENT #21=(CFSignal (CObList) "-10 Ap" 21 0 0 4522 4528 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "4522..4528") COMMENT #22=(CFSignal (CObList) "-35 Ap" 21 0 0 4545 4550 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "4545..4550") COMMENT #23=(CFSignal (CObList) "T7 Terminator Primer" 27 0 1 3369 3387 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(3369..3387)") COMMENT #24=(CFSignal (CObList) "T7 Terminator" 43 0 0 3383 3430 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "3383..3430") COMMENT #25=(CFSignal (CObList) "GROE RP3" 28 0 1 1635 1658 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(1635..1658)") COMMENT #26=(CFSignal (CObList) "GROE RP2" 28 0 0 2180 2204 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "2180..2204") COMMENT #27=(CFSignal (CObList) "GROE RP1" 28 0 0 2822 2843 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "2822..2843") COMMENT #28=(CFSignal (CObList) "RAD51 Opt" 4 0 0 71 1090 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #29=(CFSignal (CObList) "RAD51o F1" 27 0 0 457 475 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #30=(CFSignal (CObList) "RAD51o R1" 27 0 0 600 621 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #31=(CFSignal (CObList) "COLAORF2 FP/RP" 27 0 0 1127 1151 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList) COMMENT (CObList) (CObList) (CObList) (CObList) COMMENT (CTextView 0 COMMENT #32=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #33=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pCH1-RAD51Opt" 2) 5 COMMENT "" 0 4) COMMENT #34=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA Plasmid 'pCH1-RAD51Opt'" 1) COMMENT #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "pCOLADuet-1/GroE/RAD51-Opt." 1) COMMENT #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Local object" 1) COMMENT #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Creation Date: ? 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4590..5225 /vntifkey="33" /label=ColA\Ori /note="ColA Replication Origin" CDS complement(5423..6505) /vntifkey="4" /label=LacI /note="WildType LacI Gene" promoter 1156..1172 /vntifkey="30" /label=T7\Promoter /note="T7 Promoter" promoter 6629..6645 /vntifkey="30" /label=T7\Promoter /note="T7 Promoter" CDS complement(3660..4480) /vntifkey="4" /label=KanR /note="Neomycin Phosphotransferase II [NPT II/Neo]" operon 1241..3226 /vntifkey="103" /label=GroE\Operon /note="E.Coli GroE Operon [X07850.1]" protein_bind 5..25 /vntifkey="31" /label=LacO /note="LacO" protein_bind 1177..1197 /vntifkey="31" /label=LacO /note="LacO" RBS 1228..1233 /vntifkey="32" /label=RBS-2 /note="Ribosome Binding Site" RBS 58..63 /vntifkey="32" /label=RBS-1 /note="Ribosome Binding Site" misc_feature 1564..1569 /vntifkey="21" /label=RBS-3 /note="RBS-3 similar to E. coli galT RBS" CDS 1242..1535 /vntifkey="4" /label=GroES /note="Cpn10 Chaperonin GroES, Small Subunit of GroESL [Escherichia coli str. K-12 substr. MG1655] (NP_418566)" CDS 1579..3225 /vntifkey="4" /label=GroEL /note="Chaperonin GroEL [Escherichia coli DH1] (ACX41451.1)" protein_bind 3287..3331 /vntifkey="31" /label=S.Tag /note="S.Tag" misc_feature 4522..4528 /vntifkey="21" /label=-10\Ap misc_feature 4545..4550 /vntifkey="21" /label=-35\Ap primer complement(3369..3387) /vntifkey="27" /label=T7\Terminator\Primer /note="T7 Terminator Primer" terminator 3383..3430 /vntifkey="43" /label=T7\Terminator /note="T7 Terminator" primer_bind complement(1635..1658) /vntifkey="28" /label=GROE\RP3 /note="GroE RP3 Primer Binding Site" primer_bind 2180..2204 /vntifkey="28" /label=GROE\RP2 /note="GroE RP2 Primer Binding Site" primer_bind 2822..2843 /vntifkey="28" /label=GROE\RP1 /note="GroE RP1 Primer Binding Site" CDS 71..1090 /vntifkey="4" /label=RAD51\Opt /note="Human RAD51 Codon Optimized for E.coli Expression" primer 457..475 /vntifkey="27" /label=RAD51o\F1 /note="Internal Forward Sequencing Primer" primer 600..621 /vntifkey="27" /label=RAD51o\R1 /note="Internal Reverse Sequencing Primer" primer 1127..1151 /vntifkey="27" /label=COLAORF2\FP/RP /note="Forward and Reverse Vector Primers for Sequencing " BASE COUNT 1732 a 1668 c 1715 g 1530 t ORIGIN 1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag 61 gagatatacc atggcaatgc agatgcagct ggaagcgaac gcagatacct cagtggaaga 121 agaaagtttt ggtccgcaac cgattagccg cctggaacag tgcggtatca acgcaaatga 181 tgtgaaaaag ctggaagaag ctggctttca taccgttgaa gcagtcgctt atgcgccgaa 241 aaaggaactg atcaacatca agggtatcag cgaagccaaa gcagacaaga tcctggccga 301 agcggccaaa ctggttccga tgggctttac cacggcaacc gaatttcatc agcgtcgcag 361 cgaaattatc caaattacca cgggttctaa agaactggat aagctgctgc agggcggtat 421 tgaaaccggc tctatcacgg aaatgtttgg tgaatttcgc accggcaaaa cgcagatttg 481 ccacaccctg gcggtgacgt gtcaactgcc gatcgatcgt ggcggtggcg agggtaaagc 541 catgtacatt gacaccgaag gcacgtttcg tccggaacgc ctgctggctg ttgcggaacg 601 ctatggtctg agcggctctg atgtgctgga caacgttgcc tacgcacgtg ctttcaatac 661 cgatcatcag acgcaactgc tgtatcaggc gagtgccatg atggtcgaat cccgctacgc 721 gctgctgatt gtggatagcg caaccgctct gtatcgtacg gactactcag gtcgcggcga 781 actgtcggca cgtcaaatgc acctggctcg ttttctgcgc atgctgctgc gtctggcgga 841 cgaatttggt gttgccgtgg ttatcaccaa ccaggtcgtg gcgcaagtcg atggcgcagc 901 tatgttcgcg gccgacccga aaaagccgat tggtggcaat attatcgcgc acgccagtac 961 cacgcgcctg tatctgcgta aaggtcgcgg cgaaacccgt atttgcaaaa tctatgattc 1021 cccgtgtctg ccggaagccg aagccatgtt tgccatcaat gccgacggtg ttggtgacgc 1081 taaggattaa gcggccgcat aatgcttaag tcgaacagaa agtaatcgta ttgtacacgg 1141 ccgcataatc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc 1201 catcttagta tattagttaa gtataagaag gagatataca tatgaatatt cgtccattgc 1261 atgatcgcgt gatcgtcaag cgtaaagaag ttgaaactaa atctgctggc ggcatcgttc 1321 tgaccggctc tgcagcggct aaatccaccc gcggcgaagt gctggctgtc ggcaatggcc 1381 gtatccttga aaatggcgaa gtgaagccgc tggatgtgaa agttggcgac atcgttattt 1441 tcaacgatgg ctacggtgtg aaatctgaga agatcgacaa tgaagaagtg ttgatcatgt 1501 ccgaaagcga cattctggca attgttgaag cgtaatccgc gcacgacact gaacatacga 1561 atttaaggaa taaagataat ggcagctaaa gacgtaaaat tcggtaacga cgctcgtgtg 1621 aaaatgctgc gcggcgtaaa cgtactggca gatgcagtga aagttaccct cggtccaaaa 1681 ggccgtaacg tagttctgga taaatctttc ggtgcaccga ccatcaccaa agatggtgtt 1741 tccgttgctc gtgaaatcga actggaagac aagttcgaaa atatgggtgc gcagatggtg 1801 aaagaagttg cctctaaagc aaacgacgct gcaggcgacg gtaccaccac tgcaaccgta 1861 ctggctcagg ctatcatcac tgaaggtctg aaagctgttg ctgcgggcat gaacccgatg 1921 gacctgaaac gtggtatcga caaagcggtt accgttgcag ttgaagaact gaaagcgctg 1981 tccgtaccat gctctgactc taaagcgatt gctcaggttg gtaccatctc cgctaactcc 2041 gacgaaaccg taggtaaact gatcgctgaa gcgatggaca aagtcggtaa agaaggcgtt 2101 atcaccgttg aagacggtac cggtctgcag gacgaactgg acgtggttga aggtatgcag 2161 ttcgaccgtg gctacctgtc tccttacttc atcaacaagc cggaaactgg cgcagtagaa 2221 ctggaaagcc cgttcatcct gctggctgac aagaaaatct ccaacatccg cgaaatgctg 2281 ccggttctgg aagctgttgc caaagcaggc aaaccgctgc tgatcatcgc tgaagatgta 2341 gaaggcgaag cgctggcaac tctggttgtt aacaccatgc gtggcatcgt gaaagtcgct 2401 gcggttaaag caccgggctt cggcgatcgt cgtaaagcta tgctgcagga tatcgcaacc 2461 ctgactggcg gtaccgtgat ctctgaagag atcggtatgg agctggaaaa agcaaccctg 2521 gaagacctgg gtcaggctaa acgtgttgtg atcaacaaag acaccaccac tatcatcgat 2581 ggcgtgggtg aagaagctgc aatccagggc cgtgttgctc agatccgtca gcagattgaa 2641 gaagcaactt ctgactacga ccgtgaaaaa ctgcaggaac gcgtagcgaa actggcaggc 2701 ggcgttgcag ttatcaaagt gggtgctgct accgaagttg aaatgaaaga gaaaaaagca 2761 cgcgttgaag atgccctgca cgcgacccgt gctgcggtag aagaaggcgt ggttgctggt 2821 ggtggtgttg cgctgatccg cgtagcgtct aaactggctg acctgcgtgg tcagaacgaa 2881 gaccagaacg tgggtatcaa agttgcactg cgtgcaatgg aagctccgct gcgtcagatc 2941 gtattgaact gcggcgaaga accgtctgtt gttgctaaca ccgttaaagg cggcgacggc 3001 aactacggtt acaacgcagc aaccgaagaa tacggcaaca tgatcgacat gggtatcctg 3061 gatccaacca aagtaactcg ttctgctctg cagtacgcag cttctgtggc tggcctgatg 3121 atcaccaccg aatgcatggt taccgacctg ccgaaaaacg atgcagctga cttaggcgct 3181 gctggcggta tgggcggcat gggtggcatg ggcggcatga tgtaaagatc tcaattggat 3241 atcggccggc cacgcgatcg ctgacgtcgg taccctcgag tctggtaaag aaaccgctgc 3301 tgcgaaattt gaacgccagc acatggactc gtctactagc gcagcttaat taacctaggc 3361 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag 3421 gggttttttg ctgaaacctc aggcatttga gaagcacacg gtcacactgc ttccggtagt 3481 caataaaccg gtaaaccagc aatagacata agcggctatt taacgaccct gccctgaacc 3541 gacgacaagc tgacgaccgg gtctccgcaa gtggcacttt tcggggaaat gtgcgcggaa 3601 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct 3661 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata 3721 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat 3781 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct 3841 attaagccgc tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga 3901 cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag 3961 gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg 4021 attgcgcctg agcgagacga aatacgcggt cgctgttaaa aggacaatta caaacaggaa 4081 tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag 4141 gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg 4201 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc 4261 agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca 4321 gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc 4381 cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc 4441 gcggcctaga gcaagacgtt tcccgttgaa tatggctcat actcttcctt tttcaatatt 4501 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 4561 aaaataaaca aataggcatg ctagcgcaga aacgtcctag aagatgccag gaggatactt 4621 agcagagaga caataaggcc ggagcgaagc cgtttttcca taggctccgc ccccctgacg 4681 aacatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat 4741 accaggcgtt tccccctgat ggctccctct tgcgctctcc tgttcccgtc ctgcggcgtc 4801 cgtgttgtgg tggaggcttt acccaaatca ccacgtcccg ttccgtgtag acagttcgct 4861 ccaagctggg ctgtgtgcaa gaaccccccg ttcagcccga ctgctgcgcc ttatccggta 4921 actatcatct tgagtccaac ccggaaagac acgacaaaac gccactggca gcagccattg 4981 gtaactgaga attagtggat ttagatatcg agagtcttga agtggtggcc taacagaggc 5041 tacactgaaa ggacagtatt tggtatctgc gctccactaa agccagttac caggttaagc 5101 agttccccaa ctgacttaac cttcgatcaa accgcctccc caggcggttt tttcgtttac 5161 agagcaggag attacgacga tcgtaaaagg atctcaagaa gatcctttac ggattcccga 5221 caccatcact ctagatttca gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc 5281 ccatacgata taagttgtaa ttctcatgtt agtcatgccc cgcgcccacc ggaaggagct 5341 gactgggttg aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta 5401 acttacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 5461 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg 5521 tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct 5581 gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga 5641 tggtggttaa cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg 5701 agatgtccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca 5761 tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg 5821 tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt 5881 gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta 5941 atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca 6001 gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat 6061 caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat 6121 ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg 6181 ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt 6241 gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg 6301 aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg 6361 gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt 6421 ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga 6481 catcgtataa cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct 6541 atcatgccat accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct 6601 cccttatgcg actcctgcat taggaaatta atacgactca ctata //